West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Prevalence of EGFR Mutations and Clinico-Pathological Characteristics of Chilean Lung Cancer Patients148166710.31557/APJCP.2019.20.1.1ENRogerGejmanDepartment of Pathology, School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, Chile.SergioGonzalezDepartment of Pathology, School of Medicine, Pontificia Universidad Catolica de Chile, Santiago, Chile.MatiasMunoz MedelDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.0000-0002-1262-8052BrunoNerviDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.CesarSanchezDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.CarolinaIbáñezDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.JosePeñaDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.JorgeMadridDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.JuanBrionesDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.PatriciaPerezDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.MarceloGarridoDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.HectorGalindoDepartment of Hematology and Oncology, School of Medicine, Pontificia
Universidad Catolica de Chile, Santiago, Chile.0000-0001-5741-2266Journal Article20180802Background: Lung cancer (LC) is the second leading cause of cancer death in Chile, causing >3,000 deaths every<br />year. Epidemiological LC data in Chile is scarce and scattered. Here, we aimed to quantify the prevalence of Epidermal<br />Growth Factor Receptor (EGFR) gene mutations in a Chilean cancer center. These data may identify individuals that<br />could benefit from targeted therapies such as Tyrosine Kinase Inhibitors (TKIs). Methods: A total of 1,405 Biopsies<br />from 1,381 LC patients were retrospectively analyzed retrieving clinical data from EGFR mutants including age,<br />gender, histological type, smoking habits and type of EGFR mutation. We also analyzed overall survival (OS) rates.<br />Results: From all patients 21.7% had clinically relevant EGFR mutations, and a median age at diagnosis of 65 years.<br />Most were female (64%), classified as adenocarcinomas (94.5%), and non-smokers/light smokers (93.1%). The most<br />prevalent mutation was exon-19 deletions (50.6%) followed by Leucine-to Arginine 858; OS was 15 months. Clinical<br />follow-up information was available for 83 patients. The use of TKIs in these patients significantly improved OS.<br />Conclusion: The prevalence of EGFR mutations in the studied population was 21.7%, comparable to other countries<br />in Latin America. The most frequent EGFR mutation was exon-19 deletion, OS in this group was 15 months, and TKIs<br />significantly improved OS.https://journal.waocp.org/article_81667_d22ea969b8216a060dbddfb1e16fa495.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101A Review of Gastric Cancer Research in Malaysia5118180010.31557/APJCP.2019.20.1.5ENKean GheeLimDepartment of Surgery, Clinical Campus, International Medical University, Jalan Rasah, Seremban, Negeri Sembilan, Malaysia.0000-0001-6511-1826KandasamiPalayanDepartment of Surgery, Clinical Campus, International Medical University, Jalan Rasah, Seremban, Negeri Sembilan, Malaysia.Journal Article20171219Incidence rates of gastric cancer in Malaysia has declined by 48% among males and 31% among females in the latest<br />reporting period of 13 years. Malays used to have age-standardized-rates only a fifth of those in Chinese and Indians,<br />but the incidence among them is slightly rising even as the rates drop in the other races. Besides ethnicity, a low level<br />of education, high intake of salted fish and vegetables, H pylori infection and smoking are risk factors. Consumption<br />of fresh fruit and vegetable is protective. Variation in the strains of H pylori infection affect gastric cancer risk, with<br />hspEAsia isolates among Chinese appearing linked to a high incidence than with hpAsia2 or hpEurope strains among<br />Indians and Malays. It was reported in the 1980s that only about 3% of patients presented with early gastric cancer, but<br />more encouraging rates reaching 27% with Stage 1 and 2 disease have been reported in the twenty-first century from<br />leading centres. More tumours occur in the distal stomach except in Kelantan, where the incidence is low and main site<br />is the cardia. Prompt endoscopy is advocated and open access, with direct referrals, to such services using a weighted<br />scoring system should be more utilized. In view of the high rate of late disease laparoscopic staging unnecessary<br />laparotomy needs to be avoided. Late presentation of gastric cancer however, is still predominant and the mortality to<br />incidence ratio is relatively high. Besides seeking to reduce risk factors and achieve early detection, implementation<br />of improved care for patients with late disease must be promoted in Malaysia.https://journal.waocp.org/article_81800_71e59be97f649ee9b9c0d3669211cdc9.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Survival Rate of Colorectal Cancer in Iran: A Systematic Review and Meta-Analysis13218166510.31557/APJCP.2019.20.1.13ENKhadijeMaajaniDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences Tehran, Iran.MahmoudKhodadostGerash University of Medical Sciences, Gerash, Iran.Department of
Epidemiology, School of Public Health, Iran
University of Medical Sciences, Tehran, Iran.ArashFattahiDepartment of Neurosurgery, Iran
University of Medical Sciences, Tehran, Iran.EhsanShahrestanakiDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences Tehran, Iran.AliyarPirouziGerash University of Medical Sciences, Gerash, Iran.FatemeKhaliliVice-Chancellery for health, Isfahan
University of Medical Sciences, Isfahan, Iran.HamedFattahiHealth Management and Economics Research Center, Iran
University of Medical Sciences, Tehran, Iran.Journal Article20180110Background: Different studies have been conducted to estimate the survival rate of colorectal cancer in Iran but<br />there is no overall estimate of the survival rate. The aim of this study was to calculate the pooled 1, 3, and 5-year survival<br />rate of the patients with colorectal cancer in Iran. Methods: To retrieve relevant studies, we conducted a systematic<br />search in Iranian databases, including Iran Medex, Magiran, SID, and international databases such as Medlin/PubMed,<br />Scopus, and Google scholar using “Colorectal Neoplasms” and “Survival Rate” as keywords up to December 1st, 2017.<br />We used random effect model to estimate pooled 1, 3, and 5-year survival rates of the patients with colorectal cancer<br />in Iran. To assess the heterogeneity, we used Chi-squared test at the 5 % significance level (p <0.05) and I2 Index. We<br />used meta-regression and subgroup analysis to find a potential source of heterogeneity. Results: After a systematic<br />search, 196 articles were found, of the 38 studies met the eligibility criteria and are included in our meta-analysis. The<br />pooled 1, 3, and 5-year survival rates in patient with colorectal cancer were 0.84 (95% CI: 0.81-0.87), 0.64 (95%CI:<br />0.59-0.70), and 0.54 (95%CI: 0.49-0.58) respectively. The 5-year survival rate in the subgroup of women was 0.5<br />(0.44-0.56) and in male subgroup was 0.44 (0.40-0.48). In a subgroup of the tumor site, the 5-year survival rate in colon<br />cancer was 0.6 (0.49-0.75) and rectum cancer was 0.54 (0.36-0.69). In multivariable models, there was a significant<br />association between years of study and 5-year survival rate as a source of heterogeneity (β = 18.9, P=0.01). Conclusion:<br />According to the results of this study, women had a better survival rate than men, and according to the tumor site, the<br />5-year survival rate in colon cancer was better than the rectum cancer.https://journal.waocp.org/article_81665_7169b22b93325ab4b3466631dbef364a.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Protein Kinase CK2, a Potential Therapeutic Target in Carcinoma Management23328097210.31557/APJCP.2019.20.1.23ENHaiweiLianDepartment of Human Anatomy, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei, P.R, China.MinSuDepartment of Human Anatomy, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei, P.R, China.YijieZhuDepartment of Human Anatomy, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei, P.R, China.YunZhouDepartment of Gynaecology, Renmin Hospital,
Wuhan University, Wuhan, Hubei, P.R. China.Shahid HussainSoomroDepartment of Human Anatomy, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei, P.R, China.HuiFuDepartment of Human Anatomy, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei, P.R, China.Shenzhen Institute of Wuhan University, Shenzhen, China.Journal Article20180523The Protein kinase CK2 (formerly known as casein kinase 2) is a highly conserved serine/ threonine kinase<br />overexpressed in various human carcinomas and its high expression often correlates with poor prognosis. CK2 protein<br />is localized in the nucleus of many tumor cells and correlates with clinical features in many cases. Increased expression<br />of CK2 in mice results in the development of various types of carcinomas (both solids and blood related tumors, such<br />as (breast carcinoma, lymphoma, etc), which reveals its carcinogenic properties. CK2 plays essential roles in many key<br />biological processes related to carcinoma, including cell apoptosis, DNA damage responses and cell cycle regulation.<br />CK2 has become a potential anti-carcinoma target. Various CK2 inhibitors have been developed with anti-neoplastic<br />properties against a variety of carcinomas. Some CK2 inhibitors have showed good results in in vitro and pre-clinical<br />models, and have even entered in clinical trials. This article will review effects of CK2 and its inhibitors on common<br />carcinomas in in vitro and pre-clinical studies.https://journal.waocp.org/article_80972_7978cc99e1b590738ad7179e64cde176.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Factors Related to Employment Situation among Korean Cancer Survivors: Results from a Population-Based Study33408106210.31557/APJCP.2019.20.1.33ENAriLeeCollege of Nursing, The Catholic University of Korea, Seoul, Republic of Korea.Jong-EunLeeCollege of Nursing, The Catholic University of Korea, Seoul, Republic of Korea.Journal Article20170704Background: Due to the increased incidence and survival rate of cancer, the number of cancer survivors has been<br />increasing. In addition, many problems have been reported regarding the employment of these survivors. Therefore,<br />this study aims to investigate the employment situation of cancer survivors and associated factors. Methods: We<br />analyzed data from the Korean National Health and Nutrition Examination Survey (2010-2014), which is a nationwide<br />cross-sectional health survey, and included a total of 1,060 participants who were diagnosed with cancer. Multivariate<br />logistic regression analysis was conducted to investigate the factors associated with the participants’ employment<br />situation. Results: The employment rate among cancer survivors was 42.0%. The variables found to be associated with<br />being employed were male gender, younger age, higher household income level, educational level at middle school<br />level or under, and not having activity limitations. Conclusion: These findings suggest that, in addition to cancerrelated<br />factors, general characteristics such as gender, age, household income, and education level are important factors<br />associated with the employment status of Korean cancer survivors.https://journal.waocp.org/article_81062_40de5175bcca75888c1e495634d005d1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Polycythaemia Vera among Sudanese Patients with Special Emphasis on JAK2 Mutations41448168010.31557/APJCP.2019.20.1.41ENIbrahim KhidirIbrahimDepartment of Haematology, Faculty of Medical Laboratory Sciences, Al-Neelain University, Khartoum, Sudan.Department of Haematology, School of Medical Sciences, University Sains Malaysia, Health Campus, 16150
Kubang Kerian, Kelantan, Malaysia.0000-0002-8223-5369RoslineHassanDepartment of Haematology, School of Medical Sciences, University Sains Malaysia, Health Campus, 16150
Kubang Kerian, Kelantan, Malaysia.Elshazli WidaaAliDepartment of Haematology, Faculty of Medical Laboratory Sciences, Al-Neelain University, Khartoum, Sudan.AwadOmerRoyal Care International Hospital,
Khartoum, Sudan.Journal Article20170905Background: In recent years, a somatic point mutation in the Janus Kinase 2 (JAK2) gene (1849 G→T, V617F)<br />has been reported to occur in over 90% of patients with polycythemia vera (PV). Another JAK2 mutation in exon 12<br />had been described and shown capable of activating erythropoietin signaling pathways. Objective: In this study, we<br />aimed to determine the frequency of Jak2 mutations (JAK2V617F and JAK2 exon 12) as well as their relationships<br />with hematological parameters in Sudanese patients with myeloproliferative disorders (MPD). A comparison with<br />findings of published studies from other geographic regions was included. Materials and Methods: From each of<br />a total of 83 polycythaemia patients, six milliliters (ml) of venous blood were collected and processed for molecular<br />analysis and measurement of serum erythropoietin level by enzyme-linked immunoassay (ELISA). The JAK2 V617F<br />mutation was determined using an allele-specific competitive blocker (ACB) -PCR assay and High Resolution Melting<br />(HRM) analysis was applied for the JAK2 exon 12 mutation. Results: According to patients’ history and the results<br />for EPO levels, nine (10.7 %) out of 83 patients were found to have secondary polycythaemia and 74 (89.3%) PV. The<br />overall frequency of the 2 JAK2 mutations was 94.6% in our Sudanese PV patients, JAK2V617F being found in 91%<br />and JAK2 exon 12 mutations in 8.1%.Conclusion: In summary JAK2 V617F and JAK2 exon 12 mutations are very<br />common in Sudanese PC cases.https://journal.waocp.org/article_81680_f4355a17ade7ce5ed30da3f952ec8ae7.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Incidence and Risk Factors for Bone Metastasis in Non-Small Cell Lung Cancer45518109110.31557/APJCP.2019.20.1.45ENGustavo TellesDa SilvaNational Cancer Institute and Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.AnkeBergmannNational Cancer Institute and Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.0000-0002-1972-8777Luiz Claudio SantosThulerNational Cancer Institute and Federal University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.Journal Article20171021Background: Bone is a frequent site of metastasis from various primary cancers including with Non-Small Cell<br />Lung Cancer. The aim of this study was to investigate the incidence and risk factors for Bone Metastasis in patients with<br />Non-Small Cell Lung Cancer. Methodology: A cohort study was carried out in patients diagnosed with Non-Small Cell<br />Lung Cancer between 2006 and 2014 in a single cancer centre. A descriptive analysis of the population was compiled<br />based on mean ± standard deviation (SD) for continuous variables and percentage (%) for categorical variables.<br />Univariate and multivariate Cox proportional hazards regression analyses were carried out to quantify the relationship<br />between independent variables and the outcome variable (Bone Metastasis). Results: During the study period 1025<br />patients were diagnosed with Non-Small Cell Lung Cancer. Bone Metastasis was detected in 136 patients (13.2%)<br />with a mean interval between Non-Small Cell Lung Cancer diagnosis and Bone Metastasis of 8.07 months (95% CI:<br />7.68 8.45). Multivariate analysis revealed that younger age (HR 0.97; 95%CI, 0.95–0.99; p=0.002), adenocarcinoma<br />(HR 1.51; 95%CI, 1.06–2.15; p=0.021) and treatment with chemotherapy, radiotherapy or chemotherapy combined<br />with radiotherapy (HR 3.73; 95%CI, 2.30–6.05; p<0.001) were associated with the occurrence of Bone Metastasis.<br />Conclusion: The incidence of Bone Metastasis was 13.2%. Younger age, histological subtype adenocarcinoma and<br />treatment with chemotherapy, radiotherapy or chemotherapy combined with radiotherapy are independent risk factors<br />for Bone Metastasis.https://journal.waocp.org/article_81091_2b178312b8628a029f1d0cbf2d87d092.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101The Correlation of Blood Parameters with Size in Cases of Neoplastic Tumor53578107410.31557/APJCP.2019.20.1.53ENEdytaWolny-RokickaRadiotherapy Development Department in Provincial Multidisciplinary Hospital in Gorzów Wielkopolski, ul Dekerta 1, 66-400 Gorzow Wielkopolski LLC, Poland.University of Zielona Góra, Faculty of Medicine and Health Sciences, ul. Zyty 28, 65-046 Zielona Góra, Poland.JerzyWydmańskiDepartment of Radiotherapy, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Branch in Gliwice, ul Wybrzeża Armii Krajowej 15,44-101 Gliwice, Poland.AndrzejTukiendorfSocial Medicine Department, Medical University in Wrocław, ul. Bujwida 44, 50-345 Wrocław, Poland.PiotrMrózUniversity of Zielona Góra, Faculty of Computer Science, Electrical Engineering and Automation, Institute of Metrology, Electronics and Computer Science, ul. Prof. Z. Szafrana 2, 65 – 516 Zielona Góra, Poland.KatarzynaGramackaDepartment of Radiotherapy, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Branch in Gliwice, ul Wybrzeża Armii Krajowej 15,44-101 Gliwice, Poland.AgnieszkaNamysł-KaletkaDepartment of Radiotherapy, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Branch in Gliwice, ul Wybrzeża Armii Krajowej 15,44-101 Gliwice, Poland.JakubLipińskiLaboratory, Regional Clinical Hospital in Zielona Góra ul. Zyty 26, 65-001 Zielona Góra, Poland.AgniszkaZembroń-ŁacnyUniversity of Zielona Góra, Faculty of Medicine and Health Sciences, ul. Zyty 28, 65-046 Zielona Góra, Poland.Journal Article20171023Purpose: This study aimed to evaluate the impact of tumor volume on platelet counts (PLT) and mean platelet<br />volume (MPV) and involve these parameters on overall survival. Methods: It is a retrospective study of 99 patients with<br />lung cancer (confirmed histologically or cytologically). Sixty-six patients underwent radical operating treatment and 33<br />patients had only biopsies – due to the inoperable status of tumor According to the histopathology profile: non-small cell<br />carcinoma – 23%, adenocarcinoma - 23 %, squamous - 36%, small cell carcinoma -11%, carcinoid – 6%. The overall<br />survival was measured from the time of surgery to last observation or death. The tumor’s size was established based<br />on information from histopathology protocol by using model for the ellipsoid (V=4/3 π r abc). Results: KM median<br />survival time after surgery was 20 months (95% C.I. = 16–42). The survival time depends significantly on: Tumor<br />feature, MPV (p=0.03, p=0.04). Patients with normal PLT levels have longer survival time (median: 11 months) than<br />thrombocytosis group (9.5) (p=0.6). Following both the PLT and MPV, a change-point that is equal to approximately<br />18.5 cm3 (approx. 3.3 cm in diameter) stands for a segmented relationship between tumor volume and analyzed blood<br />indicators. Conclusions: After an overstepping of the change-point of tumor volume inflammatory processes start and<br />they are associated with poor prognosis. MPV may be a valuable biomarker for the diagnosis and follow up of various<br />types of carcinoma.https://journal.waocp.org/article_81074_0bda0ae3a19e58354f6e6f93c272e7ac.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Nottingham Prognostic Index is an Applicable Prognostic Tool in Non-Metastatic Triple-Negative Breast Cancer59638168110.31557/APJCP.2019.20.1.59ENOAl JarroudiService of Medical Oncology, University Hospital Mohammed VI-Oujda, Morocco.AZaimiService of Medical Oncology, University Hospital Mohammed VI-Oujda, Morocco.S ABrahmiService of Medical Oncology, University Hospital Mohammed VI-Oujda, Morocco.SAfqirService of Medical Oncology, University Hospital Mohammed VI-Oujda, Morocco.Journal Article20171130Introduction: Triple-negative breast cancer (TNBC) is characterized by a poor prognosis due to high mortality<br />and early relapse, requiring the study of its prognostic factors. Tumor size, histological grade and lymph node status<br />represent important parameters that are widely studied in breast cancer, and are retained as prognostic factors by several<br />international guidelines. The Nottingham team combined these parameters into a prognostic score called the Nottingham<br />prognostic index (NPI). In this study, we investigated the influence of NPI on outcomes in non metastatic TNBC.<br />Methodology: This retrospective cohort study included all female patients with non metastatic TNBC who received<br />treatment at the Regional Oncology Center Hassan II Oujda - Morocco, between January 2009 and December 2011.<br />The prognostic impact of the NPI on the survival curves at 5 years was studied using multivariate Cox proportional<br />hazards models. Results: The analysis of the data involved 98 patients, 39 patients (39.8%) were classed in the poor<br />prognosis group with a NPI > 5.4. The Overall survival (OS) and Disease free survival (DFS) rates at 5 years, in this<br />group, were 70 and 55.6 % respectively. After adjusting for clinic-pathological variables, a NPI > 5.4 was associated with<br />mortality (HR: 2.598, 95% CI: 1.423 – 4.744, p = 0.002) and disease progression (HR: 2.512, CI to 95%: 1.496 – 4.219,<br />p <0.001) in patients with non-metastatic TNBC. Conclusion: This retrospective cohort analysis showed that NPI was<br />an independent prognostic factor for OS and DFS at 5 years in women with non metastatic TNBC. Once validated, the<br />impact of this score on survival outcomes could be considered in the clinical management of TNBC.https://journal.waocp.org/article_81681_4b84314a45dc5acb2e75d4775861bee6.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Antimetastatic Potential of Garcinone E in Human Oral Cancer Cells65728171910.31557/APJCP.2019.20.1.65ENSheejaKLaboratory of Molecular Medicine, Division of Cancer Research, Regional Cancer Centre, Medical College, Thiruvanananthapuram,
Kerala, India.LakshmiSLaboratory of Molecular Medicine, Division of Cancer Research, Regional Cancer Centre, Medical College, Thiruvanananthapuram,
Kerala, India.Journal Article20171212Objective: Oral cancer presents as a devastating type of malignancy. It is predominant in populations with high<br />use of alcohol and various forms of tobacco as well as poor diets with low intake of fruits and vegetables. The present<br />study focused on the potential of Garcinone E to inhibit HSC-4 oral cancer cell proliferation, migration and invasion.<br />Methods: MTT and colony forming assays were performed to study antiproliferative effects of Garcinone E. Hoechst<br />staining was used to determine levels of apoptosis, with cell invasion and scratch assays conducted for migration and<br />invasion characteristics. The levels of MMPs and cytokines were quantified in Garcinone E treated cells by ELISA.<br />Results: Garcinone E inhibited the proliferation and colony forming potential of HSC-4 cells. It also suppressed<br />migration and invasion with inhibition of MMP-2 and MMP-9 expression. Moreover, it elevated IL-2 and reduced<br />IL-6 expression in HSC-4 cells. Conclusion: Our results demonstrate for the first time that Garcinone E might inhibit<br />metastasis of an oral cancer cell line by blocking invasion, migration and MMP production.https://journal.waocp.org/article_81719_0dc0fadc21812936226938a77e5f5f1f.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Immunohistochemical Evaluation of Ki-67 and Comparison with Clinicopathologic Factors in Breast Carcinomas73798096310.31557/APJCP.2019.20.1.73ENNguiessan AlphonseAmanDepartment of Pathology, School of Medicine, Alassane Ouattara University, BP V 18 Bouake, Ivory Coast.BrahimaDoukoureDepartment of Pathology, School
of Medicine, Felix H Boigny University, 01 BP V 34 Abidjan 01, Ivory Coast.Kouadio DonatienKoffiDepartment of Pathology, School of Medicine, Alassane Ouattara University, BP V 18 Bouake, Ivory Coast.Baumaney SylvanusKouiDepartment of Pathology, School
of Medicine, Felix H Boigny University, 01 BP V 34 Abidjan 01, Ivory Coast.Zie CheickTraoreDepartment of Pathology, School of Medicine, Alassane Ouattara University, BP V 18 Bouake, Ivory Coast.MohamedKouyateDepartment of Pathology, School
of Medicine, Felix H Boigny University, 01 BP V 34 Abidjan 01, Ivory Coast.IbrahimanToureDepartment of Pathology, School of Medicine, Alassane Ouattara University, BP V 18 Bouake, Ivory Coast.Ahoua BenjaminEffiDepartment of Pathology, School of Medicine, Alassane Ouattara University, BP V 18 Bouake, Ivory Coast.Journal Article20180209Background: Patients primarily received tamoxifen based on their menopausal status due to the lack of<br />immunohistochemistry. A recent study has shown that hormonal receptors were not correlated with menopausal status,<br />and thus, indicating that they present limited therapeutic and prognostic significance in breast cancer management.<br />This study aimed to evaluate Ki-67 value and analyze its association with clinicopathologic parameters in breast cancer<br />patients. Methods: The formalin-fixed paraffin-embedded breast tissue blocks of 125 patients with primary breast<br />carcinomas were subjected to immunohistochemical analysis using Ventana Benchmark® GX automated immunostainer.<br />Analysis of variance and Chi-2 test were used to examine the relationship between Ki-67 and clinicopathologic<br />variables. Results: The mean age of 125 patients included in the study was 47.7 years. The average score of Ki-67<br />was 56.0%. 84.8% of patients showed Ki-67 ≥ 14%. Mean scores of Ki-67 were correlated with grade (p = 0.006),<br />PR (p = 0.026), histological type, ER, combined ER/RP, and molecular subtype (p < 0.001). Ki-67 was independent<br />of HER2 (p = 0.402) and menopausal status (p = 0.471). The frequency of Ki-67 according to St Gallen 2011 was<br />associated with histological type (p = 0.005), grade (p = 0.005), ER (p < 0.001), combined ER/PR (p = 0.004), and<br />molecular subtype (p = 0.004). There was no significant relationship between the distribution of Ki-67 and the age of<br />the patients (p = 0.859), menopausal status (p = 0.979), PR (p = 0.149), and HER2 (p = 0.597). Conclusion: Ki-67 is<br />useful for treatment decisions in primary breast cancer patients. The high value of Ki-67 was associated with adverse<br />clinicopathologic factors. The increased Ki-67 value should be carefully investigated in triple negative patients.https://journal.waocp.org/article_80963_dd2d5308691a664add6ff9b64a81adf0.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101The Impact of Triage for Atypical Squamous Cells of Undetermined Significance with Human Papillomavirus Testing in Cervical Cancer Screening in Japan81858162210.31557/APJCP.2019.20.1.81ENHiroyukiFujiwaraDepartment of Obstetrics and Gynecology, Jichi Medical University, Yakushiji, Shimotsuke, Tochigi, Japan.MitsuakiSuzukiGynecological Cancer
Center, Shinyurigaoka, General Hospital, 255 Furusawa, Asao-ku, Kawasaki, Kanagawa, Japan.HiroyukiMorisawaDepartment of Obstetrics and Gynecology, Jichi Medical University, Yakushiji, Shimotsuke, Tochigi, Japan.MasaakiSayamaItsuki Ladies Clinic, 5-18-15 Hitotonoya,Oyama, Tochigi, Japan.KouzoKimuraKimura Ladies Clinic, 1-7-7 Gion, Shimotsuke, Oyama, Tochigi, Japan.Journal Article20180318Background: One of the features of cervical cancer screening using the combination of cytology and human<br />papillomavirus (HPV) testing is the triage for atypical squamous cells of undetermined significance (ASC-US). The<br />effectiveness of the triage has been recognized widely. However, there are few reports evaluating this triage process in<br />Japan. Material and Methods: We retrospectively examined the results of cytology and HPV co-testing for cervical<br />cancer screening in the Oyama area of Tochigi Prefecture between 2012 and 2014. Women who were ASC-US/HPV<br />positive and had cytologic abnormalities [low-grade squamous intraepithelial lesions (LSIL) or worse] were examined<br />by colposcopy. The results of the colposcopy testing were evaluated. In addition, we also examined the results of those<br />who underwent co-testing a year after a ASC-US/HPV-negative result. Results: A total of 21,342 women received<br />their first screening test during the study period, with 542 (2.5%) found to have ASC-US. Of the ASC-US-positive<br />women, 289 (53.3%) were also HPV positive. The prevalence of CIN+ (cervical intraepithelial neoplasia or higher)<br />in the ASC-US/HPV-positive group was 63.2%, with 81.8%, 16.4% and 4.8%. showing CIN 1, CIN 2 and CIN 3+,<br />respectively. The prevalence of CIN+ in the LSIL group was 66.8%, with the majority having a low risk CIN 1 (76.6%)<br />compared to CIN 2 (18.6%), and CIN 3+ (4.8%). No significant difference was observed between the LSIL and ASC-US/<br />HPV-positive groups. The prevalence of women diagnosed with CIN in the ASC-US/HPV-negative group, following<br />co-testing a year after colposcopy was low (3%). Conclusions: The ASC-US/HPV-positive group was comparable to<br />the LSIL group in terms of prevalence of CIN+ lesions. Furthermore, low CIN prevalence after one year in the ASCUS/<br />HPV-negative group provides confirmation that the screening interval could be extended. The application of HPV<br />triage (which is routine in other countries) to identify these groups would be of benefit in Japan.https://journal.waocp.org/article_81622_8b831622e26299ffa9fb8c26d31b34cd.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Estimating Premature Mortality Cost of Cancers Attributable to Obesity in Indonesia87908106410.31557/APJCP.2019.20.1.87ENBagus DwikyRiantoroDepartment of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.Susi AriKristinaDepartment of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.0000-0003-4248-6830DwiEndartiDepartment of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.Journal Article20180323Obesity is one of the risk factor of non-communicable diseases, one of them is cancer. It may lead to the escalation<br />of mortality. Indonesia is the top 10 country with the highest prevalence of cancer burden due to obesity. The chance<br />of cancer caused by obesity is estimated epidemiologic descriptive and prevalence-based estimation with obesity<br />attributable cancer mortality and Premature Mortality Cost as indicators. Obesity-related cancer mortality is calculated<br />by multiplying Obesity Attributable Fractions (OAFs) rate by cancer death rate, which OAFs rate obtained by calculating<br />obesity prevalence data and relative risk of each disease. Premature Mortality Cost was calculated by multiplying<br />obesity-related cancer mortality, life expectancy, and Indonesian average income. The highest total mortality’s rate<br />for obesity-related cancer and overweight-related cancer are colon cancer (929 deaths), ovarian cancer (599 deaths),<br />and pancreatic cancer (190 deaths). The highest value of Premature Mortality Cost are IDR 61,118 million for ovarian<br />cancer, IDR 56,651 million for colon cancer, and IDR 15,472 million for pancreatic cancer. The estimation of cancer<br />burden due to obesity shall be done to help the government determines the health programs and decrease the number<br />of cancer burden due to obesity in Indonesia.https://journal.waocp.org/article_81064_88e71786da24e0424a0d2b9640a17c48.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Effect of Induction Chemotherapy on Swallowing in Head and Neck Cancer Patients91968108510.31557/APJCP.2019.20.1.91ENJulianaPortasSLP Head and Neck Department, Barretos Cancer Hospital, Barretos-SP, Brazil.DanielaCorrêaCenter for Researcher Support, Barretos Cancer Hospital, Barretos-SP, Brazil.DéboraQueijaSLP University of Medicine, São Paulo University, São Paulo-SP, Brazil.Lidia Maria Rebolho BatistaArantesHead and Neck Oncology Departament, Barretos Cancer Hospital, Barretos-SP, Brazil0000-0001-8230-1218Luciano De SouzaVianaHead and Neck Oncology Departament, Barretos Cancer Hospital, Barretos-SP, BrazilAndré LopesCarvalhoHead and Neck Oncology Departament, Barretos Cancer Hospital, Barretos-SP, BrazilJournal Article20180411Objective: To evaluate the effect of induction chemotherapy on swallowing and swallowing-related quality of life<br />of patients with oropharyngeal, laryngeal and hypopharyngeal cancer. Study design: Prospective study of 33 patients<br />with locally advanced tumors who were eligible for treatment with neoadjuvant chemotherapy followed by radiotherapy<br />and concurrent chemotherapy. A multidimensional assessment of swallowing was performed using the following<br />tools: (1) Clinical analysis, numerical scale for general pain and painful swallowing, American Speech and Hearing<br />Association (ASHA) Functional Communication Measures (FCM) swallowing assessment scales, (2) assessment<br />of Swallowing-Related Quality of Life (The MD Anderson Dysphagia Inventory - MDADI), and (3) swallowing<br />videofluoroscopy. Results: There was a reduction in general pain scale (p=0.021), and quality of life (emotional,<br />functional and physical) improved from average limitation (score of 61-80) to minimal limitation (81-100) after<br />induction chemotherapy. According to the swallowing videofluoroscopy results, 26 (78.7%) of the patients had some<br />degree of dysphagia pre-treatment, which decreased to 21 (63.6%) after induction. Conclusion: Our results suggest<br />that induction chemotherapy improves numerous aspects of swallowing and has a positive impact on the quality of<br />life of pre-chemoradiotherapy patients.https://journal.waocp.org/article_81085_a06230e9a043be86e8cfb8aaa5bd9f7c.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Setting Priorities in Childhood Cancer in Low Income Countries Using Nominal Group Technique: Experience from an International Childhood Cancer Forum Exercise in Bangladesh971038183210.31557/APJCP.2019.20.1.97ENSyed AzizurRahmanDepartment of Health Services Administration, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.Prime University, Bangladesh.0000-002-25836037Michael EkubuOtimDepartment of Health Services Administration, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.Virtual University of Uganda, Kampala, Uganda.AminaAlmarzouqiDepartment of Health Services Administration, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.ShristeeRahmanFaculty of Land and Food System, University of British Columbia, Canada.Journal Article20180416Background and Objectives: Cancer is one of the major causes of mortality and morbidity worldwide. The<br />incidence of paediatric cancer in particular, in Bangladesh is alarming and most of these patients die without correct<br />diagnosis and adequate medical treatment (MOHFW, 2008). There is a clear disparity in access to care between rural<br />and urban areas (WHO, 2015; Rahman, 2001). There are no established formal childhood cancer registry systems<br />to help inform planning decisions across the country. Most importantly, there are no explicit priorities or methods<br />for identifying such priorities in Low and Middle Income Countries (LMICs). We used a Nominal Group Technique<br />(NGT) method during the International Childhood Cancer Forum (ICCF) for setting priorities. The following two key<br />objectives were addressed: Trialling the NGT in Bangladesh as a priority setting tool; and identify childhood cancer<br />priorities in Bangladesh. Methods: The Nominal Group Techniques (NGT) method was used to elicit information from<br />the participants of ICCF to identify priorities for research and interventions for childhood cancer care in Bangladesh.<br />Participants were divided into four groups. Each group discussed one question each. Two questions focused on cancer<br />research, and the other two focused on interventions. Results: In regards to outcomes, NGT successfully identified<br />the scale of childhood cancer care and identified priorities/action areas to address in Bangladesh. Six priorities were<br />identified and a successful collaboration for implementation has been established with several international organisations.<br />Conclusion: Nominal group technique was found to be an effective tool to identify research and intervention priorities<br />to address childhood cancer in a developing country. For resource limited countries in similar situations, they could<br />benefit from adopting this approach in healthcare settings.https://journal.waocp.org/article_81832_747f14f75bb5a96afda89d06a8ecc002.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Overweight as an Additional Risk Factor for Colorectal Neoplasia in Lean Population1051118109010.31557/APJCP.2019.20.1.105ENSureepornJangsirikulDivision of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.WasineePromratpanDivision of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.SatimaiAniwanDivision of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.NatanongKongtubDivision of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.NaruemonWisedopasDepartment of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.PinitKullavanijayaDivision of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.RungsunRerknimitrDivision of Gastroenterology, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross, Bangkok, Thailand.Journal Article20180418Background: Overweight in Thailand is not as common as in Western countries. We sought to evaluate overweight<br />as the additional risk factor that can increase the prediction of colorectal neoplasia (CRN) detection in Thais apart from<br />the Asia-Pacific Colorectal Screening (APCS) score. Methods: We prospectively enrolled asymptomatic 338 subjects<br />who underwent screening colonoscopy between November 2016 and September 2017. All risk factors according to<br />APCS, BMI and the presence of metabolic syndrome were collected. Overweight was defined as BMI ≥23 kg/m2. By<br />APCS score, subjects were categorized into 1) high-risk and 2) average-risk. Using the combination of APCS score<br />and overweight, subjects were stratified into 4 groups; high-risk with overweight (G1), average-risk with overweight<br />(G2), high-risk with normal weight (G3) average-risk and with normal weight (G4). Logistic regression analysis was<br />used to estimate the risk of detecting CRN. Results: The prevalence of CRN in the high-risk subjects was higher<br />than that of in the average-risk subjects (49%vs.32%; OR, 2.00; 95%CI, 1.17-3.41). After adjustment for APCS risk<br />factors and metabolic syndrome, overweight significantly increased the risk of detecting CRN (OR, 2.52; 95%CI,<br />1.57-4.05). Among the 4 groups, the detection rates of CRN were significantly different (G1=64%, G2=40%, G3=32%<br />and G4=21%, p<0.01). The relative risk of detecting CRN increased when G1 (OR 6.49; 95%CI, 2.87-14.67), and G2<br />(2.42; 1.39-4.21) were compared with G4. Conclusions: In addition to the APCS score, overweight is an independent<br />risk factor for detecting CRN. In Thai population, combining overweight and APCS score may be useful to improve<br />the prediction for CRN.https://journal.waocp.org/article_81090_9b795c9de97732b2ba8c74b8659e50b4.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Integrating a 92-Gene Expression Analysis for the Management of Neuroendocrine Tumors of Unknown Primary1131168195910.31557/APJCP.2019.20.1.113ENAmanChauhanDepartment of Internal Medicine, Division of Medical Oncology, University of Kentucky, Lexington, KY, United States.ZainabFarooquiUniversity of Kentucky, College of Osteopathic Medicine, Pikeville,
KY, United States.Scott RSilvaDepartment of Radiation Oncology, Stritch School of Medicine Loyola University Chicago, Maywood, IL, United States.Le AundraMurrayUniversity of Kentucky, Lexington, KY, United States.Kurt BHodgesDepartment of Pathology and Laboratory Medicine, University of Kentucky, Lexington, KY, United States.QianYuCollege of Medicine, University of Kentucky, Lexington, KY, United States.Zin WMyintDepartment of Internal Medicine, Division of Medical Oncology, University of Kentucky, Lexington, KY, United States.Arumugam KRaajesekarDepartment of Internal Medicine, Division of Medical Oncology, University of Kentucky, Lexington, KY, United States.HeidiWeissDepartment of Biostatistics, College of Public Health, University of Kentucky, Lexington, KY, United States.Department of Surgery, University of Kentucky, Lexington, KY, United States.Markey Cancer Center, University of Kentucky, Lexington, KY, United States.SusanneArnoldDepartment of Internal Medicine, Division of Medical Oncology, University of Kentucky, Lexington, KY, United States.Markey Cancer Center, University of Kentucky, Lexington, KY, United States.B MarkEversDepartment of Surgery, University of Kentucky, Lexington, KY, United States.LowellAnthonyDepartment of Internal Medicine, Division of Medical Oncology, University of Kentucky, Lexington, KY, United States.Markey Cancer Center, University of Kentucky, Lexington, KY, United States.Journal Article20180418Background: Neuroendocrine tumors (NETs) are rare tumors that can originate from any part of the body. Often,<br />imaging or exploratory surgery can assist in the identification of the tumor primary site, which is critical to the<br />management of the disease. Neuroendocrine tumors (NETs) of unknown primary constitute approximately 10-15%<br />of all NETs. Determining the original site of the tumor is critical to providing appropriate and effective treatment.<br />Methods: We performed a retrospective review of neuroendocrine tumors at our institution between 2012 and 2016<br />using a 92-gene cancer ID analysis. Results: 56 patients with NETs of unknown primary were identified. Samples<br />for 38 of the 56 underwent the 92-gene cancer ID analysis. The primary site of the tumor was identified with >95%<br />certainty in 35 of the 38 patients. Conclusion: The 92-gene cancer ID analysis identified a primary site in 92% of our<br />NETs study cohort that previously had been unknown. The results have direct implications on management of patients<br />with regard to FDA-approved treatment options.https://journal.waocp.org/article_81959_27aa51ddd6f3b548f03a0321474b1704.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Comparison of Real Cost Versus the Indonesian Case Base Groups (INZ-CBGs) Tariff Rates Among Patients of High- Incidence Cancers Under the National Health Insurance Scheme1171228186610.31557/APJCP.2019.20.1.117ENSatibiSatibiFaculty of Pharmacy, University Gadjah Mada, Indonesia.Tri MurtiAndayaniFaculty of Pharmacy, University Gadjah Mada, Indonesia.DwiEndartiFaculty of Pharmacy, University Gadjah Mada, Indonesia.I Putu TangkasSuwantaraFaculty of Pharmacy, Setyabudi University, Indonesia.Ni PutuWintarianiFaculty of Pharmacy, Setyabudi University, Indonesia.Ni Putu DewiAgustiniFaculty of Pharmacy, Setyabudi University, Indonesia.Journal Article20180424Background: The national health insurance of Indonesia has implemented the Indonesian Case Base Groups<br />(INA-CBGs) tariff rates for healthcare payment. However, there is still problem of difference between the real cost of<br />healthcare and the INA-CBGs tariff rates. This study aimed to evaluate the real cost of healthcare in comparison with<br />the INA-CBG’s tariff rates and to analyze factors associating with the real cost. The study focus on healthcare cost of<br />non-chemotherapy expenditure among patients of high-incidence cancers having chemotherapy covered by the national<br />health insurance. Methods: The study was conducted from the perspective of healthcare provider. Costs data was<br />obtained from hospital billing of Sanglah hospital, a referral hospital in Bali Provincein the period of January – July<br />2014. The data involved 383, 161, and 152 of in-patient breast cancer cases, cervical cancer cases, and nasopharyngeal<br />cancer cases, respectively. Descriptive statistic was used to analyze patients characteristics, one sample t-test was used<br />to analyze the mean difference of healthcare cost based on real cost and INA-CBG’s tariff rates, and finally, bivariate<br />analysis was used to examine relationship between patients’ characteristics and the real cost. Results: The study shows<br />there were significant differences of non-chemotherapy expenditures based on the real cost and INA-CBG’s tariff rates,<br />in which the costs were lower for the real cost. Factors which significantly associated with the real cost were number of<br />procedure, type of hospitalized room, and length of stay. Conclusions: The study supports the necessary of evaluation<br />of the INA-CBG’s tariff rates to adjust to the real healthcare expenditure. On the other hand, the hospital needs to<br />evaluate the service quality of patient treatment by optimizing budget allocated by the health insurance.https://journal.waocp.org/article_81866_aeb441752b309230cfeda7162d0d2228.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)1231308097010.31557/APJCP.2019.20.1.123ENFarzaneh SadatHosseiniMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.MojganNoroozi KarimabadMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Mohammad RezaHajizadehMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.AlirezaKhoshdelDepartment of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Soudeh KhanamaniFalahati-PourPistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Mohammad RezaMirzaeiMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.0000-0002-6283-5332Seyed MehdiMirmohamadiMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.MehdiMahmoodiMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.Department of Clinical Biochemistry, Afzalipoor Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.0000-0002-8463-8364Journal Article20180507Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the<br />cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of<br />apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS<br />and L929 cells were cultured and treated with different concentrations (0–10 mg/ml) of Cornus mass L. extract for 48<br />and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified<br />at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and<br />double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by<br />ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation<br />among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in<br />comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately<br />several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC50 value in AGS cells (evaluated<br />after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry<br />indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each<br />of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation<br />in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.https://journal.waocp.org/article_80970_fdca3e6e18781661ee347c3290bdc953.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Frequency of Polypharmacy in Advanced Cancer Patients Consulted with the Palliative Service of Imam Khomeini Hospital (Tehran), Iran, 20171311348109210.31557/APJCP.2019.20.1.131ENAmin RezaKhalediDepartment of Palliative Medicine, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.MaryamKazemiResearch Expert,
Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.MamakTahmasebiThe cancer Institute, Tehran University of Medical Sciences, Tehran, IranJournal Article20180512Background: Polypharmacy is defined as the concurrent use of more than four or five medications by an individual.<br />The prevalence of this condition has increased due to the ageing population and the related illnesses. Use of multiple<br />medications would increase the risk of side-effects, drug interactions, and medical costs. The present study aimed to<br />determine the frequency of polypharmacy in the advanced cancer patients. Methods: In this cross-sectional study, 92<br />patients with advanced cancer were selected through convenience sampling from the inpatients and outpatients who<br />referred to the Palliative Care Unit of Imam Khomeini Hospital (Tehran) in 2017. An examining physician completed a<br />researcher-made checklist for all the subjects based on the patients’ biography and medical records. Statistical analysis<br />was performed by using SPSS software (version 19.0) through descriptive and analytical tests at the significance level<br />of pin 81.5% of the patients (n=75), the most prevalent of which were psychiatric disorders. Eighty-eight percent of the<br />patients (n=81) were on at least 5 or more medications. Opioids and antacids were the most common medications used<br />by these patients. Conclusion: The frequency of polypharmacy and average number of consumed medications were<br />high in patients with advanced cancer. Studying the effectiveness of these medications can highly help the physicians<br />stop or continue prescribing such medications, and guide the focus of attention towards the drugs that can improve the<br />patients’ quality of life in the final days.https://journal.waocp.org/article_81092_1376f209b02b1b935780574c7051e6a4.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Behavior and Outcomes of Pregnancy Associated Breast Cancer1351388107110.31557/APJCP.2019.20.1.135ENKausarSulemanSection Medical Oncology,
Oncology Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.Asif HusainOsmaniSection Medical Oncology,
Oncology Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.HashemAl HashemSection Medical Oncology,
Oncology Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.TaherAl TwegieriSection Medical Oncology,
Oncology Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.DahishAjarimSection Medical Oncology,
Oncology Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.NohaJastaniyahDepartment Radiation Oncology, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.WafaAl KhayalDepartment of Breast Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.OsamaAl MalikDepartment of Breast Surgery, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.AdharAl SayedSection Medical Oncology,
Oncology Centre, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.Journal Article20180609Introduction: Pregnancy Associated Breast cancer (PABC) is associated with poor prognosis and a decreased overall<br />survival. A retrospective review was conducted to review the experience and outcome in a tertiary care hospital, and to<br />compare those seen in a matched group for year of diagnosis. Materials and Methods: This is a retrospective review<br />of a prospectively collected breast cancer registry. The study was conducted in a tertiary care hospital in Riyadh, Saudi<br />Arabia from January to Decamber 2014 . Female patients with PABC were identified and matched with similar cohort<br />of non-pregnant breast cancer patients that were diagnosed between 2001-2010. Clinical data including age, tumor<br />biology, clinical stage, follow up and outcomes (disease free survival, DFS) were analyzed and compared between the<br />two groups using SAS 9.3 and R-2.14.1 Results: A total of 110 patients in Group 1 and 114 patients in Group II were<br />analyzed. In both groups, the patient age ranged was between 20 to 45 years; the median follow up was 34 months in<br />PABC and 54 months in non-pregnant cohort. PABC were statistically more likely to be triple negative (p value-0.05) and<br />diagnosed at advanced stage (stage 3 and 4) (p value-0.02). There was no difference in the occurrence of Her-2 positive<br />disease. In pregnant patients there was a 5-year survival rate of 65% compared to non-pregnant cohort of 82% with p<br />value of 0.002 and DFS was also 47.5% versus 65.4% with a p value .002 which is statistically significant. Conclusion:<br />Pregnancy associated breast cancer (PABC) is diagnosed at a more advanced stage and tends to be triple negative and<br />they are associated with a worse DFS and overall survival. Early detection during pregnancy may improve outcome.https://journal.waocp.org/article_81071_36f5ed2170cd03da2d9fb4db9a80c1ee.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Single Nucleotide Polymorphism in Adiponectin Gene and Risk of Pancreatic Adenocarcinoma1391438167810.31557/APJCP.2019.20.1.139ENAmal AhmedMohamedDepartment of Biochemistry and Molecular Biology, National Hepatology and Tropical Medicine Research Institute, Cairo University, Cairo, Egypt.HebaOmarDepartment
of Endemic Medicine and Hepato-gastroenterology, Faculty of Medicine, Cairo University, Cairo, Egypt.Mohamed F AbdelGhaffarDepartment of
Surgery, Faculty of Medicine, Ain Shams University, Cairo, Egypt.Mohamad SaeedMarieDepartment
of Endemic Medicine and Hepato-gastroenterology, Faculty of Medicine, Cairo University, Cairo, Egypt.Mohamed Ezz El ArabRamadanDepartment of Internal Medicine, Ahmed Maher Teaching Hospital, Cairo, Egypt.Soha MTalimaClinical Oncology Department, Kasr Al-Ainy Center
of Clinical Oncology and Nuclear Medicine (NEMROCK), Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt.Mostufa ELDalyClinical Oncology Department, Kasr Al-Ainy Center
of Clinical Oncology and Nuclear Medicine (NEMROCK), Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt.SehamMahmoudTropical
Medicine Department, El Sahel teaching hospital, Cairo, Egypt.Journal Article20180622Introduction: Adiponectin is anti-inflammatory and anti-tumor cytokine secreted exclusively from adipocytes. There<br />is a growing evidence of association between adiponectin gene polymorphism and development of pancreatic cancer.<br />The current study aimed at evaluation of the possible association between selected adiponectin gene polymorphism and<br />the risk of pancreatic cancer. Methods: Prospective case-control study included 77 patients (29 women and 48 men)<br />with biopsy-proven pancreatic adenocarcinoma and 97 healthy control. Blood samples from all included participants<br />were genotyped for 3 single nucleotide polymorphism (SNPs) of adiponectin genes (rs1501299C>A, rs266729C>G and<br />rs2241766G>T) by PCR. Clinical, biochemical, and radiological data analyzed. Results: We demonstrated a significant<br />association between the three studied SNPs (rs1501299, rs266729, and rs2241766) and increased risk of pancreatic<br />adenocarcinoma (p<0.001). Furthermore, in clinical correlation analysis, Patients with rs2241766 polymorphism<br />have a lower frequency of lymph node involvement (p 0.05). Smoking and older age were independent predictors of<br />pancreatic adenocarcinoma. Conclusion: We provided evidence that variants in adiponectin gene might influence the<br />development and progression of pancreatic cancer.https://journal.waocp.org/article_81678_58e832780eaf454cc583b90d6c098327.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Identification of Key Candidate Genes and Pathways for Relationship between Ovarian Cancer and Diabetes Mellitus Using Bioinformatical Analysis1451558166610.31557/APJCP.2019.20.1.145ENYiSunDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.HuangXiaoyanDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.LiuYunDepartment of Gynaecology and Obstetrics,
Kailuan General Hospital, Tangshan, Hebei, China.LiuChaoqunDepartment of Nutrition, School of Medicine, Jinan University, Guangzhou,
Guangdong, China.WenJialingGuangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun
Yat-sen University, Guangzhou, China.YangLiuDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.ZhaoYingqiDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.YiPeipeiDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.PengJunjunDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.LuYuanmingDepartment of Toxicology, Guilin Medical University School of Public Health, Guilin, China.Journal Article20180625Ovarian cancer is one of the three major gynecologic cancers in the world. The aim of this study is to find the<br />relationship between ovarian cancer and diabetes mellitus by using the genetic screening technique. By GEO database<br />query and related online tools of analysis, we analyzed 185 cases of ovarian cancer and 10 control samples from<br />GSE26712, and a total of 379 different genes were identified, including 104 up-regulated genes and 275 down-regulated<br />genes. The up-regulated genes were mainly enriched in biological processes, including cell adhesion, transcription of<br />nucleic acid and biosynthesis, and negative regulation of cell metabolism. The down-regulated genes were enriched in<br />cell proliferation, migration, angiogenesis and macromolecular metabolism. Protein-protein interaction was analyzed<br />by network diagram and module synthesis analysis. The top ten hub genes (CDC20, H2AFX, ENO1, ACTB, ISG15,<br />KAT2B, HNRNPD, YWHAE, GJA1 and CAV1) were identified, which play important roles in critical signaling<br />pathways that regulate the process of oxidation-reduction reaction and carboxylic acid metabolism. CTD analysis<br />showed that the hub genes were involved in 1,128 distinct diseases (bonferroni-corrected P<0.05). Further analysis by<br />drawing the Kaplan-Meier survival curve indicated that CDC20 and ISG15 were statistically significant (P<0.05). In<br />conclusion, glycometabolism was related to ovarian cancer and genes and proteins in glycometabolism could serve as<br />potential targets in ovarian cancer treatment.https://journal.waocp.org/article_81666_21ec5aabfa24eba655698d1694814448.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Breast Cancer Detection using Crow Search Optimization based Intuitionistic Fuzzy Clustering with Neighborhood Attraction1571658169710.31557/APJCP.2019.20.1.157ENParvathavarthiniSDepartment of Computer Technology, Kongu Engineering College, Perundurai, Tamilnadu, India.Karthikeyani VisalakshiNDepartment of Computer Science, Government Arts and Science College, Kangeyam, Tamilnadu, India.ShanthiSDepartment of Computer Applications, Kongu Engineering College, Perundurai, Tamilnadu, India.Journal Article20180709Objective: Generally, medical images contain lots of noise that may lead to uncertainty in diagnosing the<br />abnormalities. Computer aided diagnosis systems offer a support to the radiologists in identifying the disease affected<br />area. In mammographic images, some normal tissues may appear to be similar to masses and it is tedious to differentiate<br />them. Therefore, this paper presents a novel framework for the detection of mammographic masses that leads to<br />early diagnosis of breast cancer. Methods: This work proposes a Crow search optimization based Intuitionistic fuzzy<br />clustering approach with neighborhood attraction (CrSA-IFCM-NA) for identifying the region of interest. First order<br />moments were extracted from preprocessed images. These features were given as input to the Intuitionistic fuzzy<br />clustering algorithm. Instead of randomly selecting the initial centroids, crow search optimization technique is applied<br />to choose the best initial centroid and the masses are separated. Experiments are conducted over the images taken from<br />the Mammographic Image Analysis Society (mini-MIAS) database. Results: CrSA-IFCM-NA effectively separated<br />the masses from mammogram images and proved to have good results in terms of cluster validity indices indicating<br />the clear segmentation of the regions. Conclusion: The experimental results show that the accuracy of the proposed<br />method proves to be encouraging for detection of masses. Thus, it provides a better assistance to the radiologists in<br />diagnosing breast cancer at an early stage.https://journal.waocp.org/article_81697_ca44d6033e1b69817cb98fab5f0d574e.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Active Contour Based Segmentation and Classification for Pleura Diseases Based on Otsu’s Thresholding and Support Vector Machine (SVM)1671738171610.31557/APJCP.2019.20.1.167ENMMalathiDepartment of Electronics and Instrumentation, Saveetha Engineering College, Chennai, India.0000-0002 1748-1280PSinthiaDepartment of Electronics and Instrumentation, Saveetha Engineering College, Chennai, India.KJalaldeenDepartment of Electronics and Instrumentation, Sethu Institute of Technology, Madurai, India.Journal Article20180715Objective: Generally, lung cancer is the abnormal growth of cells that originates in one or both lungs. Finding the<br />pulmonary nodule helps in the diagnosis of lung cancer in early stage and also increase the lifetime of the individual.<br />Accurate segmentation of normal and abnormal portion in segmentation is challenging task in computer-aided diagnostics.<br />Methods: The article proposes an innovative method to spot the cancer portion using Otsu’s segmentation algorithm. It<br />is followed by a Support Vector Machine (SVM) classifier to classify the abnormal portion of the lung image. Results:<br />The suggested methods use the Otsu’s thresholding and active contour based segmentation techniques to locate the<br />affected lung nodule of CT images. The segmentation is followed by an SVM classifier in order to categorize the<br />affected portion is normal or abnormal. The proposed method is suitable to provide good and accurate segmentation<br />and classification results for complex images. Conclusion: The comparative analysis between the two segmentation<br />methods along with SVM classifier was performed. A classification process based on active contour and SVM techniques<br />provides better than Otsu’s segmentation for complex lung images.https://journal.waocp.org/article_81716_5a3845c2d7cbce2b69eef681fd6f7bce.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt1751848179910.31557/APJCP.2019.20.1.175ENAhmadZakiDepartment of Chemical Pathology, Medical Research Institute, Alexandria
University, Egypt.Ragaa AbdelkaderRamadanDepartment of Chemical Pathology, Medical Research Institute, Alexandria
University, Egypt.0000-0002-1557-0212PacintMoezDepartment of Clinical Pathology, Faculty of Medicine, Alexandria University, Egypt.HalaGhareebDepartment of Chemical Pathology, Medical Research Institute, Alexandria
University, Egypt.AhmadElkarmoutyDepartment of Experimental and Clinical Surgery, Medical Research Institute, Alexandria University, Egypt.Journal Article20180715Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzed<br />the plasma proteomic profiling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatography<br />magnetic beads (MB-HIC8) separation followed by Matrix assisted laser desorption ionization/ time of flight mass<br />spectrometry (MALDI-TOF MS). Results: ClinProTools software identified 92 peaks that differed among the analyzed<br />groups, 33 peaks were significantly different (P < 0.05). Of those, 22 peaks were up-regulated while 11 peaks were<br />down-regulated in BC patients compared with the healthy controls. Three peptide ion signatures (m/z 1,570.31, 1,897.4<br />and 2,568.17) were provided by the Quick Classifier model to discriminate BC patients from healthy control subjects<br />with 96.4% accuracy. External validation was performed by an independent group and this achieved a sensitivity of<br />100% and a specificity of 76.9%. Conclusion: MALDI-TOF MS has good analytical performance in distinguishing<br />BC patients from healthy controls.https://journal.waocp.org/article_81799_0a45dba6ceef92586c4393c6e66fb247.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Anti-Proliferative Effects of Methanol and Water Extracts of Pyrrosia piloselloides on the Hela Human Cervical Carcinoma Cell Line1851928171110.31557/APJCP.2019.20.1.185ENMohd DasukiSul ‘ainDepartment of Biomedicine, School of Health Sciences, University
Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia.0000-0002-0063-7648FashihahZakariaDepartment of Biomedicine, School of Health Sciences, University
Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia.Muhammad FaridJohanDepartment of Haematology, School of Medical Sciences, University
Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia.0000-0001-6344-0220Journal Article20180717Background: Cervical cancer is one of the most commonly diagnosed neoplasms and a leading cause of cancer<br />death among females worldwide. Limitations with conventional medical treatments have driven researchers to<br />search for alternative approaches using natural products. This study aimed to detemine potential anti-proliferative<br />effects of methanol and water extracts of Pyrrosia piloselloides (P. piloselloides) on the HeLa cell line. Methods:<br />3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to determine IC50<br />concentrations and apoptosis analysis was by flow cytometry. To identify chemical compounds in the extracts, gas<br />chromatography-mass spectrometry (GC-MS) was employed. Results: P. piloselloides methanol extracts (PPME) showed<br />antiproliferative effects on HeL awith an IC50 of 16.25μg/mL while the P. piloselloides water extract (PPWE) was without<br />influence. Neither extract showed any significant effects on apoptosis. GC-MS analysis, revealed 5-hydroxymethylfurfural<br />(23.1%), allopurinol (8.66%) and 3, 5-dihydroxy-6-methyl-2,3-dihydropyran-4-one (7.41%) as major components in<br />the PPME, while sulfolan-3-ol (10.1%), linoleic acid (9.06%) and β-sitosterol acetate (7.98%) predominated in the<br />PPWE case. Conclusion: This first study of P. piloselloides showed PPME to exert potent anti-proliferative effect on<br />HeLa cell lines. Further research now needs to be performed to establish the mechanisms of inhibition.https://journal.waocp.org/article_81711_da9eeafab87b5cdac3de9d48b1267356.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101MiR-126 Modulates Angiogenesis in Breast Cancer by Targeting VEGF-A -mRNA1931978014510.31557/APJCP.2019.20.1.193ENLaylaAlhasanDepartment of Biology, College Education for Pure Sciences, Thi-Qar University, Nasiriya, Iraq.Journal Article20180718Background: Breast cancer is most serious reasons of women death around worldwide result in increasing its<br />morbidity and mortality. MicroRNAs are considered as significant regulators of cancer biological processes. The main<br />aim of this study is restoration of miR-126 could lead to modulate breast cell line and impairs their proliferation by<br />targeting vascular endothelial growth factor gene (VEGF-A). Methods: Breast cancer cell line (MCF7) was transfected<br />by miR-126 lipofectamine and negative miR control for 24 hr. Cytotoxic effects of miR-126 lipofectamine were<br />determined by cell viability assay. Cell proliferation and cell cycle were quantitatively measured using PicoGreen<br />assay and DAPI stain-flow cytometer analysis. For further investigation, Taq-Man real time PCR assay was performed<br />to detect relative VEGF-A and miRNA-126 level. Results: MiR-126 was overexpressed in treated breast cancer cell<br />(MCF7) compared with control cells. miR-126 expression has been associated –with a decrease in cell proliferation<br />and arrested MCF7 cells at G1 phase. The study found that vascular endothelial growth factor is regulated by miR-<br />126. Hence, VEGF-A is considered as functional vital and direct target to miR-126 in breast cancer cell line (MCF7).<br />Conclusions: This study provided that manipulated miR-126 level may suggest a novel therapeutic approach in breast<br />cancer treatment. However, an animal models study is needed to address and prove predictive ability of miR-126 on<br />breast cancer controlling.https://journal.waocp.org/article_80145_3d95f88981abde20c14d67a136243380.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Molecular Mechanism of Cancer Susceptibility Associated with Fok1 Single Nucleotide Polymorphism of VDR in Relation to Breast Cancer1992068109410.31557/APJCP.2019.20.1.199ENSanaRazaDepartment of Biosciences, Integral University, Lucknow, India.AnupamDhasmanaHimalayan School of Biosciences and Cancer Research Institute, Swami Rama Himalayan University, Dehradun, India.Madan Lal BrahmaBhattDepartment of Radiotherapy, King George's Medical University, IndiaMohtashimLohaniCollege
of Applied Medical Sciences, University of Jazan, Saudi Arabia.Jamal MArifDepartment of Biosciences, Integral University, Lucknow, India.Journal Article20180726Breast cancer is the leading cause of death among women worldwide. It is a multi-factorial disease caused by<br />genetic and environmental factors. Vitamin D has been hypothesized to lower the risk of breast cancer via the nuclear<br />vitamin D receptor (VDR). Genetic variants of these vitamin D metabolizing genes may alter the bioavailability of<br />vitamin D, and hence modulate the risk of breast cancer. Materials and Methods: The distribution of Fok1 VDR gene<br />(rs2228570) polymorphism and its association with breast cancer was analysed in a case–control study based on 125<br />breast cancer patients and 125 healthy females from North Indian population, using PCR-RFLP. An In silico exploration<br />of the probable mechanism of increased risk of breast cancer was performed to investigate the role of single nucleotide<br />polymorphisms (SNPs) in cancer susceptibility. Results: The Fok1 ff genotype was significantly associated with an<br />increased risk of breast cancer (p=0.001; χ2=13.09; OR=16.909; %95 CI=2.20 - 130.11). In silico analysis indicated<br />that SNPs may lead to a loss in affinity of VDR to calcitriol, and may also cause the impairment of normal interaction<br />of liganded VDR with its heterodimeric partner, the retinoid X receptor (RXR), at protein level, thereby affecting target<br />gene transcription. Conclusion: Breast cancer risk and pathogenesis in females can be influenced by SNPs. SNPs in<br />VDR may cause alterations in the major molecular actions of VDR, namely ligand binding, heterodimerization and<br />transactivation. VDRE binding and co-activator recruitment by VDR appear to be functionally inseparable events that<br />affect vitamin D-elicited gene transcription. This indicates that breast cancer risk and pathogenesis in females may be<br />influenced by SNPs.https://journal.waocp.org/article_81094_5384bdfe54329a6b4c4eb1d91297bc72.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Breast Cancer Screening Participation of Women with Chronic Diseases in Korea: Analysis of the 2012 Korean National Health and Nutrition Examination Survey2072138195810.31557/APJCP.2019.20.1.207ENLaurenceTwizeyimanaGraduate School of Cancer Science and Policy, National Cancer Center, Goyang, Republic of Korea.0000-0002-5850-3597YeolKimGraduate School of Cancer Science and Policy, National Cancer Center, Goyang, Republic of Korea.0000-0003-1142-1559Journal Article20180731Chronic disease morbidity is a concern for cancer research. Contradictory results have been reported concerning<br />adherence to breast cancer screening among patients with chronic diseases. The study was conducted to assess the<br />adherence to breast cancer screening among women with chronic diseases in Korea. It was a cross sectional and population<br />based study; the data came from the Korean National Health and Nutrition Examination Survey (KNHANES) 2012.<br />Participation in breast cancer screening was analyzed among women who had at least one of eight chronic diseases<br />(hypertension, diabetes, cancer, dyslipidemia, stroke, depression, osteoarthritis and asthma). Pearson’s chi-squared test<br />and multiple logistic regression analysis were performed using STATA version 14. A total 2,404 women aged 40 years<br />or older were included in analysis. Among them, 77.3% had experienced breast cancer screening. In logistic regression<br />model, adherence to breast cancer screening was lower in women who have ever been diagnosed as diabetes mellitus<br />(odds ratio (OR)= 0.47, 95% confidence interval (95%CI) = 0.31-0.72), asthma (OR=0.44, 95%CI=0.24-0.82) and<br />higher in women who have ever been diagnosed as dyslipidemia (OR= 1.85, 95%CI= 1.27-2.69), osteoarthritis (OR=<br />1.42, 95%CI= 1.31-2.06) and cancers (OR= 2.70, 95%CI= 1.26-5.79) compared to women without those chronic<br />diseases. Based on treatment of chronic diseases, lower participation in breast cancer screening was observed in women<br />who were on treatment of diabetes mellitus (OR= 0.48, 95%CI= 0.31-0.74), asthma (OR= 0.41, 95%CI= 0.15-0.92)<br />and stroke (OR= 0.37, 95%CI= 0.14-0.97), and higher in women who were on treatment of dyslipidemia (OR= 1.37,<br />95%CI=1.42-2.14). In conclusion, low participation rate in breast cancer screening in women with some chronic diseases<br />was identified. The results from this study may provide an important contribution for helping to maintain and increase<br />participation in cancer screening of patients with chronic diseases.https://journal.waocp.org/article_81958_dca68de5a1c79e444532cc74f66cd56c.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Studying the Impact of Golgi Protein 73 Serving as a Candidate Biomarker in Early Diagnosis for Hepatocellular Carcinoma among Saudi Patients2152208108810.31557/APJCP.2019.20.1.215ENRanda Mohamed AhmedFaragHealth Sciences Research Center (HSCR), Princess Nourah bint
Abdulrahman University (PNU), Kingdom Saudi Arabia.DujanaAl AyobiDepartment of Biology, Princess Nourah bint Abdulrahman University (PNU), Kingdom Saudi Arabia.Khalid AAlsalehDepartment of Medicine, Oncology Centre, King Khalid University Hospital, King Saud
University, Kingdom Saudi Arabia.Hye-JooKwonDepartment of Molecular Biology, Princess Nourah bint
Abdulrahman University (PNU), Kingdom Saudi Arabia.AfafEL-AnsaryCentral Labe, King Saud University Riyadh, Kingdom Saudi Arabia.Emad AnwarDawoudFaculty of Medicine, EL-Azher University and
Specialist Physician, Oncology Clinic-Medical Affaies, Tawam Hospital, AL Ain, UAE.Journal Article20180801Background: Due to the prevalence of Hepatocellular carcinoma (HCC) in Saudi Arabia, using new markers to<br />give best diagnostic performance than alpha-feto protein (AFP) are important in early diagnosis. The aim of this work<br />was to compare the significance between serum and mRNA Golgi glypican73 (GP-73) as newly identified diagnostic<br />and prognostic markers for HCC among Saudi patients. Materials and Methods: A total of 300 subjects were divided<br />into: 250 blood samples where 145 samples from HCC, 105 samples from chronic liver cirrhosis (CLC) and 50 normal<br />controls were investigated for serum GP73 (sGP73) by ELISA. GP-73 mRNA from peripheral blood mononuclear cells<br />was amplified by RT-PCR. The sensitivity and specificity of both techniques was compared. Results: Serum Golgi<br />glypican 73 was significantly higher in HCC group compared to cirrhotic and normal controls (p<0.001). Sensitivity<br />and specificity were 95% for sGP-73, 100% and 90% for Golgi glypican 73 mRNA. The combination of sensitivity<br />between AFP and sGP73 was 80% and 95% respectively. Conclusion: Both serum Golgi glypican-73 and GP-73Mrna<br />are good diagnostic biomarkers for early detection of HCC in Saudi patients. RT-PCR is more accurate and sensitive<br />(100%) than ELISA (95%) in detecting Golgi glypican 73.https://journal.waocp.org/article_81088_1fb31b1dca1e55866b19c8e30aa62cce.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Bioinformatics Analysis of Key Genes and Pathways for Medulloblastoma as a Therapeutic Target2212278010910.31557/APJCP.2019.20.1.221ENFatemeShaanbanpour AghamalekiDepartment of Cellular-Molecular Biology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-8078-4129BehrouzMollashahiDepartment of Cellular-Molecular Biology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University. Tehran, Iran.NikaAghamohammadiDepartment of Dental Sciences,, Shahid Beheshti University, Tehran, Iran.NematollahRostamiDepartment of Hematology and Medical Oncology, Shahid Modarres Hospital, Shahid Beheshti University of
Medical Sciences, Tehran, Iran.ZeinabMazloumiDepartment of Biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.HamidrezaMirzaeiCancer Research Center, Shohadae Tajrish Hospital, Shahid Beheshti University of
Medical Sciences, Tehran, Iran.AfshinMoradiDepartment of
Pathology, Shohadae Tajrish Hospital, Shahid Beheshti University of
Medical Sciences, Tehran, Iran.MojganSheikhpourDepartment of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur
Institute of Iran, Tehran,AbolfazlMovafaghDepartment of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Journal Article20180808Introduction: One of the major challenges in cancer treatment is the lack of specific and accurate treatment in<br />cancer. Data analysis can help to understand the underlying molecular mechanism that leads to better treatment.<br />Increasing availability and reliability of DNA microarray data leads to increase the use of these data in a variety of<br />cancers. This study aimed at applying and evaluating microarray data analyzing, identification of important pathways<br />and gene network for medulloblastoma patients to improve treatment approaches especially target therapy. Methods:<br />In the current study, Microarray gene expression data (GSE50161) were extracted from Geo datasets and then analyzed<br />by the affylmGUI package to predict and investigate upregulated and downregulated genes in medulloblastoma. Then,<br />the important pathways were determined by using software and gene enrichment analyses. Pathways visualization<br />and network analyses were performed by Cytoscape. Results: A total number of 249 differentially expressed genes<br />(DEGs) were identified in medulloblastoma compared to normal samples. Cell cycle, p53, and FoxO signaling pathways<br />were indicated in medulloblastoma, and CDK1, CCNB1, CDK2, and WEE1 were identified as some of the important<br />genes in the medulloblastoma. Conclusion: Identification of critical and specific pathway in any disease, in our case<br />medulloblastoma, can lead us to better clinical management and accurate treatment and target therapy.https://journal.waocp.org/article_80109_bd544cc54f7ae96419121e8d7a441657.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Application of aSi-kVCBCT for Volume Assessment and Dose Estimation: An Offline Adaptive Study for Prostate Radiotherapy2292348179810.31557/APJCP.2019.20.1.229ENArunGandhiDepartment of Radiation Oncology, Yashoda Hospital, Hyderabad, India.Research and Development Centre, Bharathiar University, Coimbatore, India.SubramaniVellaiyanResearch and Development Centre, Bharathiar University, Coimbatore, India.Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.ShanmugaSubramanianDepartment of Radiation Oncology, Yashoda Hospital, Hyderabad, India.Research and Development Centre, Bharathiar University, Coimbatore, India.Shanmugam ThirumalaiSwamyDepartment of Radiation Oncology, Yashoda Hospital, Hyderabad, India.KalaSubramanianDepartment of Radiation Oncology, Yashoda Hospital, Hyderabad, India.AnantharamanAyyalusamyDepartment of Radiation Oncology, Yashoda Hospital, Hyderabad, India.Research and Development Centre, Bharathiar University, Coimbatore, India.Journal Article20180810Objective: The purpose of this study is to develop a method to estimate the dose using amorphous silicon detector<br />panel cone beam computed tomography (aSi-kVCBCT) for the OARs and targets in prostate radiotherapy and to compare<br />with the actual planned dose. Methods: The aSi-kVCBCT is used widely in radiotherapy to verify the patient position<br />before treatment. The advancement in aSi-kVCBCT combined with adaptive software allows us to verify the dose<br />distribution in daily acquired CBCT images. CBCT images from 10 patients undergoing radical prostate radiotherapy<br />were included in this study. Patients received total dose of 65Gy in 25 fractions using volumetric modulated arc therapy<br />(VMAT). aSi-kVCBCT scans were acquired before daily treatment and exported to smart adapt software for image<br />adaptation. The planning CT is adapted to daily aSi-kVCBCT images in terms of HU mapping. The primary VMAT<br />plans were copied on to the adapted planning CT images and dose was calculated using Anisotropic Analytic Algorithm<br />(AAA). The DVH is then used to evaluate the volume changes of organs at risk (OAR), the actual dose received by<br />OARs, CTV and PTV during a single fraction. Results: The normalized volume of the bladder and rectum ranged<br />from 0.70–1.66 and 0.70–1.16 respectively. The cumulative mean Sorensen–Dice coefficient values of bladder and<br />rectum were 0.89±0.04 and 0.79±0.06 respectively. The maximum dose differences for CTV and PTV were 2.5% and<br />-4.7% and minimum were 0.1% and 0.1% respectively. Conclusion: The adapted planning CT obtained from daily<br />imaging using aSi-kVCBCT and SmartAdapt® can be used as an effective tool to estimate the volume changes and<br />dose difference in prostate radiotherapy.https://journal.waocp.org/article_81798_5bb72d2fd10be00c8452615440ebf350.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Cytogenetic Abnormalities in Multiple Myeloma Patients at a Tertiary Healthcare Center in India2352418179710.31557/APJCP.2019.20.1.235ENPerumalGovindasamyDepartment of Centralised Molecular Diagnostics, Apollo Hospitals, Chennai-6, Tamil Nadu, India.PrabuPanduranganDepartment of Haematology, Apollo Hospitals, Chennai-6, Tamil Nadu, India.AnilTarigopulaDepartment of Centralised Molecular Diagnostics, Apollo Hospitals, Chennai-6, Tamil Nadu, India.RamaManiDepartment of Centralised Molecular Diagnostics, Apollo Hospitals, Chennai-6, Tamil Nadu, India.ChandraR SamuelDepartment of Genetics, Dr. ALMPGIBMS, University of Madras, Chennai-113, Tamil Nadu, India.0000-0002-2278-6433Journal Article20180815Objective: Multiple myeloma (MM) is a clinically and genetically heterogeneous plasma cell neoplasm. The<br />prognosis of MM patients is dependent on several factors including the patient’s age, the stage of disease and genetic<br />alterations. This study aimed to determine the frequency of common chromosomal abnormalities and their significance in<br />MM patients referred to a tertiary healthcare center in India. Methods: Fluorescence in situ hybridization on interphase<br />nuclei from bone marrow cells using seven MM-specific probes for recurrent aberrations was performed in a total of<br />215 newly diagnosed patients. Results: Chromosomal abnormalities were detected in 161 (74.9%) MM patients in<br />this study. The most frequent aberration was trisomy(ies) involving only gain of chromosomes in 48 (22.3%) cases.<br />A translocation involving the IGH gene alone or accompanied by trisomy(ies) or by monosomy 13/13q deletion or by<br />both was registered in 80 (37.2%) patients. Atypical patterns such as a deletion of the IGH variable segment (IGHv)<br />on the derivative chromosome 14 or on the native (normal) chromosome 14, biallelic deletion of IGHv, deletion of<br />the IGH constant segment on the rearranged chromosome14 and extra fusions were noticed in 21 (9.8%) patients<br />with an IGH rearrangement. Monosomy 13/deletion 13q was identified singly or as part of a complex karyotype in<br />74 patients (34.4%). Clonal heterogeneity and additional abnormalities including TP53 deletion and monosomies of<br />chromosomes 4, 9, 14 and 16 were recorded in 18.6% and 16.3% of patients respectively. Patients with abnormalities<br />exhibited plasmacytosis, reduced hemoglobin value and high level of ß2-microglobulin. Conclusions: A lower median<br />age and a low frequency of IGH translocations particularly t(11;14) and chromosome 13 abnormalities suggest ethnic<br />diversity. Further investigations on genetic alterations including IGH deletions will contribute to improved insights<br />into the biology of myeloma disease, risk stratification and patient management.https://journal.waocp.org/article_81797_c5b6be7363d307f008d713c076934421.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Useful Application of Immunostaining to Malignant Pleural Effusion among Lao People in Vientiane Capital, Lao PDR2432488167010.31557/APJCP.2019.20.1.243ENSoulidethVilayvongDepartment of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.KiyomiHandoDepartment of Clinical Laboratory
Sciences, Sanyo Women's College, Hiroshima, Japan.MasakiSekineDepartment of Pathology, Medical Hospital, Tokyo Medical and Dental University, TokyoThitsamayLuangxayDepartment of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.PhetsamoneArounlangsyDepartment of Pathology, Faculty of Medicine, University of Health Care
Sciences, Vientiane Capital, Lao PDR, Laos.PheangvilayXaysomphetDepartment of Pathology, Faculty of Medicine, University of Health Care
Sciences, Vientiane Capital, Lao PDR, Laos.PhetlammoneXayaphetDepartment of Pathology, Faculty of Medicine, University of Health Care
Sciences, Vientiane Capital, Lao PDR, Laos.HatthakoneThavisoukDepartment of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.YurieSoejimaDepartment of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.MasanobuKitagawaDepartment of Comprehensive Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.MotojiSawabeDepartment of Molecular Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.0000-0002-7212-2547Journal Article20180817Background: Pathology and laboratory medicine (PALM) services are limited in low-resource countries, such as<br />Lao PDR. Patients with malignant pleural effusion (MPE) are not properly diagnosed and treated in these situations.<br />The purpose of this study is to confirm the usefulness of immunocytochemistry in MPE to identify the histological<br />type and probable primary site of cancer of MPE and to discuss its usefulness in low-resource countries, such as Laos.<br />Methods: We retrospectively reviewed glass slides of pleural effusion sent to the Department of Pathology at the<br />University of Health Sciences from the central hospitals for cytological screening from January 2012 to December<br />2016. The cytological review, cell transfer and immunocytochemical staining were performed at Tokyo Medical and<br />Dental University. Among 81 cases of MPE from Laos, 66 cases of malignant tumors that contained enough tumor<br />cells were included in this study, and the slides were screened with 14 primary antibodies to classify the histological<br />type and identify the probable primary site of carcinoma. Results: Among the 66 cases, 34 cases (52%) were of female<br />patients, and 32 cases (48%) were of male patients. The patients’ ages ranged from 28 to 88 years with an average of 58<br />years. The immunocytochemical study identified 32 cases (49%) of primary lung adenocarcinoma, two cases (3%) of<br />malignant mesothelioma, one case (1.5%) of breast/gynecological carcinoma, one case (1.5%) of T cell lymphoma, and<br />one case (1.5%) of B cell lymphoma. Twenty-nine cases (43.5%) were classified as carcinoma not otherwise specified.<br />Pulmonary small cell carcinoma/squamous cell carcinoma and metastatic colon, prostate, and liver carcinoma were not<br />identified among the cases. Conclusions: Immunocytochemistry is a useful ancillary method in MPE diagnostics. This<br />method could be applied in the pathological laboratories in low- or middle-resource countries, such as Laos.https://journal.waocp.org/article_81670_09e984de99792ec5a7160942440d9862.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Stromal Expression of CD10 by Immunohistochemistry in Odontogenic Keratocyst (OKC), Dentigerous and Radicular Cysts and Its Correlation with Local Recurrence and Aggressive Behaviour2492538161910.31557/APJCP.2019.20.1.249ENAnamAliDepartment of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan.MuhammadAsifConsultant Histopathologist, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan.BismahAhmadConsultant Histopathologist, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan.0000-0002-1199-8238ShahidJamalHead of Department
Histopathology, Watim Medical College, Rawalpindi, Pakistan.IramAliHamdard College of Medicine and Dentistry, Karachi, Pakistan.MuhammadKhadimDepartment of Histopathology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan.Journal Article20180820Objective: To assess and compare the stromal expression of CD10 in OKC, dentigerous and radicular cysts. Materials<br />and Methods: This comparative, cross sectional study was conducted at Armed Forces Institute of Pathology (AFIP),<br />Rawalpindi, from Jan 2017 to Dec 2017. Total sixty cases comprising 20 of each OKC, Dentigerous and Radicular cysts<br />were included in this study. Hematoxylin and eosin (H and E) sections were performed followed by immunohistochemical<br />staining for CD10 antibody. Expression of CD10 was evaluated and compared. Results were analyzed by using SPSS<br />version 20.0. Chi Square test was performed with P value < 0.05 was considered as significant. Results: A total of<br />60 cases, 20 of each OKC, dentigerous and radicular cysts were taken. In our study, 38 (63.3%) male and 22 (36.7%)<br />female patients with the mean age of 32 ± 15 (mean ± SD) were included. Percentage of CD10 positive cells were<br />highest in sub-epithelial stroma of OKC (95% cases) as compared to radicular and dentigerous cysts (60 and 70%) with<br />highest number of cases showing intense staining in OKC 13(65%) as compared to other odontogenic cysts i-e 4(20%)<br />and 2 (10%) respectively. There was a statistically significant association between odontogenic cysts and proportional<br />score, intensity score and combined score of stromal CD10 expression (P=0.009, p=0.001 and p=0.000). Conclusion:<br />In this study, we found that highest stromal CD10 expression in OKC as compared to dentigerous and radicular cyst,<br />which might be due to aggressive behaviour and increased risk of recurrence in OKC. Expression of CD10 marker will<br />further aid the clinician to plan appropriate surgical intervention and keep regular follow-ups to identify recurrences.https://journal.waocp.org/article_81619_2c2d9f537a617ec09181f881004980a6.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Protease Cargo in Circulating Exosomes of Breast Cancer and Ovarian Cancer Patients2552628171310.31557/APJCP.2019.20.1.255ENSvetlana NTamkovichInstitute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.Novosibirsk State University, Novosibirsk, Russia.Natalia VYunusovaCancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Science, Tomsk, Russia, Tomsk, Russia.Siberian State Medical University, Tomsk, Russia.ElenaTugutovaCancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Science, Tomsk, Russia, Tomsk, Russia.Anton KSomovInstitute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.Ksenia VProskuraInstitute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.Regional Clinical Oncological Hospital, Novosibirsk, Russia.Larisa AKolomietsCancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Science, Tomsk, Russia, Tomsk, Russia.Siberian State Medical University, Tomsk, Russia.Marina NStakheyevaCancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Science, Tomsk, Russia, Tomsk, Russia.Alina EGrigor’evaInstitute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.Pavel PLaktionovInstitute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, Russia.Novosibirsk Research Institute of Circulation Pathology Academician E.N. Meshalkin, Novosibirsk, Russia.Irina VKondakovaCancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Science, Tomsk, Russia, Tomsk, Russia.0000-0003-0907-4615Journal Article20180822Background: As is known, exosomes play an important role in promoting progression of cancers by increasing<br />its invasive potential. The aim of this study was to evaluate the levels of tetraspanine-associated (ADAM-10) and<br />tetraspanine-nonassociated proteases (20S proteasomes) in exosomes from culture medium, plasma exosomes of<br />patients with breast tumors and plasma and ascites of ovarian tumor patients. Methods: MCF-7 and SVO-3 culture<br />mediums and blood samples from healthy females (n = 30, HFs), patients with diffuse dyshormonal dysplasia of the<br />breast (n=28, BBTPs), breast cancer patients (n=32, BCPs), borderline ovarian tumor patients (n=20, BOTPs) and<br />blood and ascites samples ovarian cancer patients (n=35, OCPs) were included in the study. Exosomes from plasma,<br />ascites and culture mediums were isolated and characterized in according to Extracellular Vesicles Society. The<br />expression levels of 20S proteasome and ADAM-10 in exosomes were determined using flow cytometry and western<br />blot analysis, correspondingly. Results: The subpopulation composition of the exosomes from MCF-7 culture medium<br />and from blood plasma of HFs and breast diseases patients is similar, however CD9/CD24 subpopulation significantly<br />increased at cell supernatant. The similar results was obtained for exosomes from SVO-3 medium and blood plasma<br />and ascites of ovary tumor patients, but CD9/CD24 subpopulation significantly decreased at cells and illness samples,<br />however CD63/CD24 exosomes increased significantly from cell supernatant. 20S proteasome level is significantly<br />increased in exosomes from MCF-7 and SVO-3 culture medium, breast tumor patients and OCPs plasma in comparison<br />to HUVEC culture medium and HFs plasma samples. At CD9-positive exosomes from BCPs plasma and MCF-7 was<br />reveal a high expression of ADAM-10 and low expression is from BBDPs plasma and ovarian tumor patients plasma/<br />ascites samples. Exosomes from ascites OCP had high expression of ADAM-10 in the CD24-positive subpopulation.<br />Conclusion: Breast and ovarian cancer development is connected with functioning of immune proteasome forms in<br />plasma and ascites exosomes, while increased ADAM10 expression at CD9-positive exosome was associated with<br />breast cancer and at CD24-positive subpopulation – with ovarian cancer. Obtained data confirm role of exosomal<br />proteases in tumor progression.https://journal.waocp.org/article_81713_5de6d9c24facd37fb33a65b8943b371d.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Novel Combination of Mesenchymal Stem Cell-Conditioned Medium with Sorafenib Have Synergistic Antitumor Effect of Hepatocellular Carcinoma Cells2632678108710.31557/APJCP.2019.20.1.263ENImanSeyhounTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.0000-0002-8216-7933SaiehHajighasemlouTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.Food and Drug Control Laboratory (FDCL),Iran Ministry of Health and Medical Education,Tehran, Iran.JafarAiTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.FaezehHosseinzadehTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.MiladMirmoghtadaeiSchool of Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran.Seyed MoeinSeyhounDepartment of Microbiology, Qom Branch,Islamic Azad University, Qom, Iran.BenyaminParsehTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.ShahrokhAbdolahiTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.ZeinabGhazvinianTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.MahdiShadnoushNutrition and Food Technology, Shahid Beheshti
University of Medical Sciences, Tehran, Iran.JavadVerdiTissue Engineering and Applied cell Sciences,Tehran University of Medical Sciences,Tehran, Iran.Journal Article20180829Objective: Hepatocellular carcinoma (HCC) is the most common liver malignancy. Sorafenib is the first-line<br />systemic treatment for advanced HCCs. However, due to safety concerns, researchers are now looking for ways to boost<br />the efficacy of the medication. One approach for reducing toxicity is combining sorafenib with other agents so that a<br />lower dose of sorafenib is required. Mesenchymal stromal cells (MSCs) can have an inhibitory effect on HCC tumor<br />growth. Mesenchymal Stem Cell-Conditioned Medium (MSC-CM) is the substance extracted from MSC culture and<br />contains most of the potential cytokines secreted by MSCs. We, therefore, anticipated a synergistic Antitumor Effect<br />of sorafenib in Combination with MSC-CM. In this study, we used HepG2 as our target cell lines. Methods: HepG2<br />cells were treated with sorafenib alone and with sorafenib + MSC-CM. CCK-8 assay was used to evaluate and compare<br />the inhibition of cell growth between the two groups with different treatments. Results: The combination treatment of<br />cell lines with sorafenib and MSC-CM had significantly reduced the values of IC50 compared to the use of sorafenib<br />alone (3.4 vs. 2.7 respectively). Conclusion: This study suggests that a combination of sorafenib with MSC-CM can<br />synergistically suppress the growth of HCC cells.https://journal.waocp.org/article_81087_6299f1d5e666cfb2da8b0c54990ebd70.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101PTEN Genetic and Epigenetic Alterations Define Distinct Subgroups in North Indian Breast Cancer Patients2692768161810.31557/APJCP.2019.20.1.269ENZakiaKazimDepartment of Biosciences, Jamia Millia Islamia, New Delhi, India.KhushnumaWahabiDepartment of Biosciences, Jamia Millia Islamia, New Delhi, India.AhmadPerwezDepartment of Biosciences, Jamia Millia Islamia, New Delhi, India.PawanindraLalDepartment of Surgery, Maulana Azad Medical College,New Delhi, India.Moshahid AlamRizviDepartment of Biosciences, Jamia Millia Islamia, New Delhi, India.Journal Article20180830Background: Breast cancer is a heterogeneous disease that can be subdivided on the basis of histopathological<br />features, genetic alterations, and gene-expression profiles. PTEN gene is considered an established tumor suppressor gene<br />in different types of cancer including breast cancer. However, the role of PTEN alterations in north Indian breast cancer<br />has not been explored especially in defining a group with distinct histological factors. Methodology: 181 sporadic breast<br />cancer and their adjacent normal tissues were included in the present study. We analyzed methylation and LOH through<br />MS-PCR and microsatellite markers respectively. While, for PTEN protein expression, we used immunohistochemistry.<br />All the molecular findings were correlated with the clinicopathological parameters of the patients to underline clinical<br />relevance. Results: We found that LOH and methylation of the PTEN promoter were significantly associated with<br />loss of PTEN protein expression, while, PTEN mutation was a rare event. Furthermore, out of 46 double hit cases (i.e.,<br />having both methylation and LOH), 70% (32/46) cases showed complete loss of PTEN expression (P= 0.0249). Both<br />LOH and PTEN promoter methylation were associated significantly with age and clinical stage, while, methylation<br />and loss of PTEN expression were associated with high grade and Her-2 negativity. In addition, a quadruple (ER/PR/<br />Her-2 and PTEN) negative group with distinct features was found. Conclusion: The pattern of PTEN expression and<br />its correlation with the clinical parameters indicates that loss of PTEN expression defines a clinical group with distinct<br />features. Hence, PTEN expression provides differential therapeutic strategies for north Indian breast cancer.https://journal.waocp.org/article_81618_70eac94629fd01729c91e39cc61ac653.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Expression of Vascular Endothelial Growth Factor and Its Receptors in Thyroid Nodular Hyperplasia and Papillary Thyroid Carcinoma: A Tertiary Health Care Centre Based Study2772828108610.31557/APJCP.2019.20.1.277ENNur HidayatiMohamad Pakarul RazyDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.Wan FaiziahWan Abdul RahmanDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.0000-0001-9280-4257Thin ThinWinPathology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.0000-0002-6468-5761Journal Article20180901Introduction: Vascular endothelial growth factor (VEGF) is an angiogenic factor that plays an important role in<br />thyroid cancer. VEGF is known to have high affinity to VEGF receptors such as VEGFR-1 (Flt-1) and VEGFR-2 (KDR).<br />Papillary thyroid carcinoma (PTC) is the most common thyroid cancer and studies showed the increasing incidence of<br />PTC arising in nodular hyperplasia. Targeted therapy on these growth factors and receptors are used in management<br />of both differentiated and undifferentiated thyroid carcinoma. This study aims to determine the expression of VEGF<br />and VEGF receptors (VEGFR) in thyroid nodular hyperplasia and PTC. Methods: A cross-sectional study based on<br />paraffinized archival tissue blocks of 113 nodular hyperplasias and 67 PTC from the thyroidectomy specimens in<br />the year of 2003 to 2014. The tissue sections were then stained by immunohistochemistry for VEGF, VEGFR-1 and<br />VEGFR-2. The lymph node involvement and extrathyroid extension also were determined. Results: The mean age of<br />PTC patients was 44.7±15.8 years and nodular hyperplasia were 42.2±13.6 years. There was a statistical difference<br />of VEGFR-1 (p=0.028) and VEGFR-2 (p=0.003) expression between nodular hyperplasia and PTC. However, no<br />significant difference of VEGF expression (p=0.576) between both diseases. Co-expression of VEGF and VEGFR-1<br />was significant in both nodular hyperplasia (p=0.016) and PTC (p=0.03), meanwhile no relevant relationship for VEGF<br />and VEGFR-2 expression (p>0.05). No significant association (p>0.05) between lymph node status and extrathyroid<br />extension with age groups, gender, VEGF and VEGFR expression. Conclusions: VEGF, VEGFR-1 and VEGFR-2<br />showed overexpression in both nodular hyperplasia and PTC. The expression of VEGFR-1 and VEGFR-2 are more<br />significant in PTC with relevant co-expression of VEGF and VEGFR-1. Therefore, the inhibition of VEGFR offers a<br />promising prospect for tumour management in thyroid carcinoma.https://journal.waocp.org/article_81086_1f28145c38bae680e98e973c8f74827b.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Is Periodontitis Independently Associated with Potentially Malignant Disorders of the Oral Cavity?2832878097310.31557/APJCP.2019.20.1.283ENMeghashyamBhatDepartment of Public Health Dentistry, Sharavathi Dental College and Hospital, Shimoga, India.0000-0001-5243-7560SreevidyaBhatDepartment of Public Health
Dentistry, Srinivas Institute of Dental Sciences, Mangalore, India.KayeRoberts-ThomsonAustralian Research Centre for Population Oral Health,
School of Dentistry, University of Adelaide, Australia.LocGiang DoAustralian Research Centre for Population Oral Health,
School of Dentistry, University of Adelaide, Australia.Journal Article20180923Objective: To examine if periodontitis is independently associated with oral potentially malignant disorders<br />(OPMD) in a rural Indian adult population aged 35-54 years. Methods: A population-based cross-sectional study was<br />conducted in rural India from 2011 to 2012. Multistage stratified cluster random sampling was followed to recruit 1401<br />participants aged 35-54 years. Face-to-face interviews were conducted to collect data on sociodemographic factors,<br />tobacco and alcohol use. Oral examinations were done to record OPMD, periodontal findings and missing teeth.<br />Univariate, bivariate and multivariable analyses were achieved using SPSS version 16 Chicago, SPSS Inc. OPMDs<br />(leukoplakia, erythroplakia, oral submucous fibrosis (OSF) and suspicious malignant lesion) whether present or absent<br />were selected as outcome variables; age, sex, socioeconomic factors, smoking, tobacco-chewing status, alcohol use,<br />and periodontitis were considered as the predictor variables. Results: Among 873 participants, 44 demonstrated 47<br />lesions. Leukoplakia (n=21), erythroplakia (n=3), OSF (n=21), and suspicious malignant lesions (n=2) were present,<br />strongly associated with past tobacco-chewing status [OR=9.22 (2.57-33.15)], current tobacco-chewing status [OR=15.49<br />(6.20-38.74)] and moderate/severe periodontitis [OR=3.19 (1.11-9.12)]. Conclusion: Periodontitis is a risk indicator for<br />OPMD, independent of socioeconomic factors and tobacco-chewing. Tobacco-chewing status, both past and current,<br />was strongly associated with OPMD in our rural Indian population.https://journal.waocp.org/article_80973_d6fcecfff46a74c9b51384b6a078b0f1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Tumor Protein p53 and K-ras Gene Mutations in Peruvian Patients with Gallbladder Cancer2892948179610.31557/APJCP.2019.20.1.289ENTatianaVidaurreInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.SandroCasavilcaInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.0000-0001-8406-739XPaolaMontenegroInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.HenryGomezInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.MónicaCalderónInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.JeannieNavarroInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.JessicaAramburuInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.EbertPoquiomaInstituto Nacional de Enfermedades Neoplásicas, Lima, Peru.YasuoTsuchiyaDivision of Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.TakaoAsaiDivision of Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.Department of Clinical Engineering and Medical Technology, Niigata University of Health and Welfare, Niigata, Japan.YoichiAjiokaDivision of Molecular and Diagnostic Pathology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.AyakoSatoDivision of Molecular and Diagnostic Pathology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.ToshikazuIkomaDivision of Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.Faculty of Health and Medical Sciences, Hokuriku University, Kanazawa, Ishikawa, Japan.KazutoshiNakamuraDivision of Preventive Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.Journal Article20180924Background: Recent studies have shown that genetic alterations are associated with the effect of patient geographic<br />location on gallbladder cancer development. Peru has a high incidence of gallbladder cancer, but causative factors<br />have not yet been identified. We examined the frequency of mutations in TP53 and K-ras genes in Peruvian patients<br />with gallbladder cancer, and compared this with data from Bolivia, Hungary, Chile, and Japan, which have a high<br />gallbladder cancer incidence. Methods: DNA was extracted from formalin-fixed paraffin-embedded gallbladder tissue<br />sections of 30 gallbladder cancer patients (9 men and 21 women) obtained using microdissection. Mutations in exons<br />5 to 8 of TP53 and codons 12, 13, and 61 of K-ras were examined using direct sequencing. Results: TP53 mutations<br />were observed in 10 (33.3%) of patients, but K-ras mutations were absent. Nine (90%) TP53 mutations were point<br />mutations (7 missense and 2 silent mutations), and the most frequent substitution was a G:C to A:T transition. G:C to<br />A:T transitions at the CpG site or G:C to T:A transversions were found in one patient each. No significant differences<br />were found in the frequency of TP53 and K-ras mutations among patients in the 5 countries. Conclusions: Our findings<br />suggest that endogenous mechanisms and exogenous carcinogens may affect the carcinogenic process in Peruvian<br />gallbladder cancer patients, similar to that in Bolivian patients. Further studies with a larger sample size are needed<br />to clarify these findings.https://journal.waocp.org/article_81796_a016572e206419c7aaf60f7f03e13a7b.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Efficacy and Safety of Microwave Ablation (MWA) for Hepatocellular Carcinoma (HCC) in Difficult Anatomical Sites in Egyptian Patients with Liver Cirrhosis2953018171410.31557/APJCP.2019.20.1.295ENAhmad FSolimanDepartment of Endemic Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.Mahmoud MAbouelkhairDepartment of Endemic Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.Maha SHasab AllahDepartment of Endemic Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.Nabil MEl-KadyDepartment of Endemic Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt.Wafaa MEzzatDepartment of Internal Medicine, National Research Center,
Cairo, Egypt.Haitham AGabrDepartment of Internal Medicine, National Research Center,
Cairo, Egypt.Ehab HElsayedDepartment of Internal Medicine, National Research Center,
Cairo, Egypt.Ahmed ISalehDepartment of Internal Medicine, National Research Center,
Cairo, Egypt.AmiraKamelDepartment of Pathology, Thedor Bilharz Research Institute, Giza, Egypt.0000-0002-5586-5672Journal Article20180924Background and aim: Imaging guided microwave ablation (MWA) for hepatocellular carcinoma (HCC) has<br />become a widely used method over recent years. Tumors close to the diaphragm, gastrointestinal tract, gallbladder,<br />pancreas, hepatic hilum and major bile duct or vessels are generally considered relative contraindications for microwave<br />ablation. This study was conducted to assess the effectiveness and safety of ultrasonography-guided MWA in treating<br />patients with HCC in difficult anatomical sites in comparison to those in conventional sites. Patients and methods:<br />Eighty-eight patients were included and divided into two groups: the study group of 44 with 46 lesions lying <5mm<br />from the diaphragm, hepatic capsule, gall bladder (GB) or large vessel; and the control group of 44 patients with<br />50 lesions in non-risky sites. Each lesion was ablated using an ultrasound guided microwave probe using a detailed<br />protocol. Results: Most of the patients were males, with a mean age of 57.8 years. In the study group, two patients<br />had lesions adjacent to the GB, twelve were perivascular and 32 were subcapsular. The overall successful ablation<br />rates were 84.8% and 92% in the study and control groups, respectively. Within the study group, ablation rates were<br />100%, 75% and 87.5% for lesions close to the GB, perivascular lesions and subcapsular lesions, respectively. One<br />patient developed a subcutaneous abscess, with good outcome after proper treatment. Fever, pain and asymptomatic<br />pleural effusion were reported after ablation without statistically significant difference between the groups or among<br />subgroups. In conclusion: MWA for HCC in difficult anatomical sites is as effective and safe as for ordinary sites.https://journal.waocp.org/article_81714_a9e14e61f7e843c4ef19b43f8d6998c9.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101Anticancer Effect of Cisplatin-Loaded Poly (Butylcyanoacrylate) Nanoparticles on A172 Brain Cancer Cells Line3033098026210.31557/APJCP.2019.20.1.303ENMohsenChianiDepartment of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.0000-0001-6413-8752AttabakToofani MilaniDepartment of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.MahdiehNematiDepartment of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.JalalRezaeidianDepartment of Biology, Parand Branch, Islamic Azad University, Parand, Iran.HosseinEhsanbakhshDepartment of Biology, Science and Research Branch, Islamic Azad University , Tehran, Iran.ZohreAhmadiFaculty of Health, Qom University of Medical Sciences, Qom, Iran.EbrahimMazloomiCellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.VahidehSadeghiDepartment of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.AzimAkbarzadeh KhiyaviDepartment of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.Journal Article20180929Background: Drug delivery systems have been designed to achieve targeted delivery and control the release rate<br />of the drugs. A serious challenge associated with drug delivery systems is the presence of the blood-brain barrier which<br />limits drugs penetration. In the current study, the effects of cisplatin nanoparticles on A172 brain cancer cell line were<br />investigated. Methods: Cisplatin nanoparticles were produced by miniemulsion polymerization technique and their<br />properties were evaluated. Drug release assay was performed to characterize the nanoparticles’ properties. Here, we<br />examined the effects of cisplatin nanoparticles and free form of cisplatin on A172 cancer cell line. MTT assay was<br />performed for different concentrations of the drug. To measure the apoptosis rate in A172 cell line in the presence<br />of cisplatin nanoparticles or its free from, Annexin V staining method was used. Results: Our results indicated that<br />loading type of cisplatin was physical loading and only 4.7% of cisplatin was released after 68 h. Furthermore, MTT<br />assay showed that cisplatin nanoparticles in all concentrations had more cytotoxic effects on the cells comparing with<br />the free form of cisplatin and control groups. We also showed that cisplatin nanoparticles could increase apoptosis<br />in cancer cells more than the drug in the free form by using flow cytometry technique. Conclusion: Overall, these<br />findings proved that cisplatin loaded on poly (Butylcyanoacrylate) nanoparticles, was more efficient than the free form<br />of cisplatin in treating A172 cancer cell line.https://journal.waocp.org/article_80262_f0de57d85eed6a5f16c8e58177ef6cd8.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736820120190101EGFR inhibitor C225 Increases the Radio-Sensitivity of Human Breast Cancer Cells3113198172010.31557/APJCP.2019.20.1.311ENZhifengYaoDepartment of Oncology, Nanjing First Hospital, Nanjing Medical University,Nanjing, Jiangsu Province, China.Department of Radiation Oncology, The Second
Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.PengPengDepartment of Nursing, Nanjing Health College of Jiangsu Union Technical Institute, Nanjing, Jiangsu Province, China.DanghuiXuDepartment of Medical Imaging, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province, China.XuejunZhouDepartment of Medical Imaging, The Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.ZhiyaoPanDepartment of Basic Medical Sciences, Zhejiang University, Hangzhou, Zhejiang Province, China.ZhanfengLiDepartment of Medical Imaging, Nanjing Health College of Jiangsu Union Technical Institute, Nanjing, Jiangsu Province, China.JianxinYaoDepartment of Medical Imaging, Nanjing Health College of Jiangsu Union Technical Institute, Nanjing, Jiangsu Province, China.JinfeiChenDepartment of Oncology, The Third Clinical Medical School of Nanjing Medical University, Nanjing First Hospital of Nanjing Medical University,Nanjing, Jiangsu Province, China.Department of Oncology, The Affiliated Taikang Xianlin Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China.Journal Article20181024Objective: This study was undertaken to investigate the effect of C225 on the radio-sensitivity of MDA-MB-231 cells<br />line and to disclosure underlying mechanism. Methods: CCK8 assay was used to measure the proliferation inhibition<br />of C225 on MDA-MB-231 cells. The combined effects of C225 plus radiation on the proliferation of MDA-MB-231<br />cells were also evaluated by CCK-8 assay. The clonogenic assay was performed to evaluate the cell surviving fractions<br />and to determine the radio-sensitizing effect of C225 on MDA-MB-231 cells. The apoptosis and cell cycle distribution<br />were analyzed by flow cytometry. Western blot analysis was used to detect the expression of p-EGFR, p-Akt, p-P38, and<br />caspase-3. Results: C225 had an inhibiting effect on the proliferation of cells in a concentration-dependent manner. The<br />cloning formation capacity was decreased in C225 plus radiation group. C225 increased radio-sensitivity of cells and<br />led to cell cycle arrest in G0/G1 phase markedly. Cells treated with C225 and radiation predominantly exhibited G0/G1<br />phase arrest and significant decreased in the fraction of cells in the S phase. Moreover, C225 and radiation significantly<br />increased the apoptosis rate of cells. Decreased cell proliferation was further supported by the down-regulation of p-EGFR<br />and its downstream singling pathway proteins such as p-Akt and p-P38. The up-regulation of the Caspase-3 expression<br />in C225 plus radiation group revealed that C225 could increase radiation-inducing cell apoptosis. Conclusion: C225<br />could increase the radio-sensitivity of cells, which may be due to the anti-proliferative synergistic effect between C225<br />and radiation as well as the down-regulation of the PI3K/Akt signaling pathway.https://journal.waocp.org/article_81720_04755848f76ac59fdf7f72ebbd81a465.pdf