West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Hemato- Oncology Care in COVID-19 Pandemic: Crisis within a Crisis117311758909110.31557/APJCP.2020.21.5.1173ENTulikaSethDepartment of Clinical Hematology, All India Institute of Medical Sciences, Delhi, India.AbhishekShankarDepartment of Radiation Oncology, Lady Hardinge Medical College & SSK Hospital, Delhi, India.0000-0001-6491-0079ShubhamRoyDepartment of Pediatrics, Sitaram Bhartia Institute of Science and Research, Delhi, India.DeepakSainiCancer Control and Prevention Division, Indian Society of Clinical Oncology, Delhi, India.0000-0002-3552-7932Journal Article20200507https://journal.waocp.org/article_89091_04d2c6b8ac96fe06ee79f6fe78b3a9dd.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Coffee Consumption and Colon Cancer Risk: A Meta- Epidemiological Study of Asian Cohort Studies117711798907010.31557/APJCP.2020.21.5.1177ENJong-MyonBaeDepartment of Preventive Medicine, Jeju National University College of Medicine, Jeju, Republic of Korea.0000-0003-3080-7852Journal Article20200208Objective: A systematic review reported that coffee consumption would decrease risk of colon cancer in Asian women. But the systematic review arises the issue of duplication, so that a meta-epidemiological study was conducted. Methods: The selection criteria were defined that a prospective cohort follow-up study conducted to evaluate coffee consumption and risk of colon cancer in Asian and showed adjusted relative risk and its 95% confidence interval. In order to conduct meta-analysis, the highest versus lowest method was applied to extract relative risk and its 95% confidence intervals of the highest category. Random effect model was applied if I-squared value was over 50%. Results: After avoiding duplication, 9 cohort data were selected for meta-analysis. The summary relative risk (and their 95% confidence intervals) [I-square value] were 0.90 (95% CI: 0.79-1.03) [0.0%] in men, and 0.64 (95% CI: 0.36-1.15) [65.9%] in women, respectively. Conclusions: The results suggest that coffee consumption is not associated with the risk of colon cancer in Asian men and women. The findings of this study are consistent with the results of two systematic reviews conducted under the same hypothesis and selection criteria. Additional epidemiological studies are needed for the inflection of colon cancer risk as the dose of coffee increases and the difference in the protective effect by sex.https://journal.waocp.org/article_89070_feaea45fd3ab56ad52d431406c7d1d35.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Alternation of β-catenin and CD44s Immunoexpression in Different Histopathological Grades of Oral Squamous Cell Carcinoma118111858907210.31557/APJCP.2020.21.5.1181ENMassoumehZargaranDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran.0000-0002-8843-2590Journal Article20190316Objective: Cell-cell adhesion molecules play an essential role in cell growth and differentiation. β-catenin and CD44s are two adhesion molecules which their expression changes are correlated to loss of differentiation and gain of an invasive epithelial phenotype. Oral squamous cell carcinoma (OSCC) is the most common malignancy of oral cavity. Aim of this study was to compare β-catenin and CD44s expression in different histopathological grades of OSCC. Methods: β-catenin and CD44s expression were evaluated in 10 well differentiated OSCC (group A) and 10 moderately/poorly differentiated OSCC (group B) using immunohistochemistry. Results: β-catenin membranous and nuclear/cytoplasmic expression were significantly different between groups A and B. CD44s membranous expression was insignificant amongst the two groups. Conclusion: Expression of β-catenin and CD44s alter in different histopathological grades of OSCC. It seems that more rate of aberrant cytoplasmic and/nuclear expression and less membranous expression of β-catenin can lead to significantly lower degree of cell differentiation in OSCC. <br /> https://journal.waocp.org/article_89072_d4c578b462ad1e2b7a8f8a2878c6f526.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Indocyanine Green Fluorescence Versus Blue Dye or Radioisotope Regarding Detection Rate of Sentinel Lymph Node Biopsy and Nodes Removed in Breast Cancer: A Systematic Review and Meta-Analysis118711958906310.31557/APJCP.2020.21.5.1187ENSarunThongvitokomarnDepartment of Surgery, Panyananthaphikkhu Chonprathan Medical center, Srinakharinwirot University, Nonthaburi, Thailand.0000-0002-3519-3290NuanphanPolchaiDivision of Head Neck and Breast Surgery, Department of Surgery, Faculty of Medicine, Siriraj Hospital, Mahidol University,
Bangkok, Thailand.0000-0002-5496-3844Journal Article20191226Background: Either blue dye (BD) or radioisotope (RI) is mainly used for sentinel lymph node biopsy (SLNB) in breast cancer patients. Unlike the BD, RI has lower false-negative rate of SLNB. However, its lymphoscintigraphy, difficulty in preoperative injection, and undetected sentinel lymph nodes in some cases cause surgeons to rely only on BD. Currently, indocyanine green (ICG) fluorescence method (ICG-SLNB) is increasingly used as an alternative to the conventional mapping methods in many centers. This systematic review compared ICG with the conventional method of BD or RI in terms of detection rate of SLNB and the number of sentinel lymph nodes (SLNs) removed in. Methods: We searched all relevant studies published between January 2000 and October 2019. All data on for evaluation of SLN detection rate, number of SLNs removed per patient, and tumor positive rate of SLNB were extracted. Results: A total of 30 studies, including 4,216 SLN procedures were retrieved. There was a statistically significant difference between ICG and BD method in terms of SLN detection rate (OR, 6.73; 95% CI, 4.20-10.78). However, there was no significant difference between ICG and RI in this regard (OR, 0.90; 95% CI, 0.40-2.03). The number of SLNs removed per patient were 2.35 (1.46-5.4), 1.92 (1.0-3.64), and 1.72 (1.35-2.08) for ICG, BD, and RI, respectively. Only in 8 studies, the tumor positive rates in SLNB could be analyzed (ICG, 8.5-20.7%; BD, 12.7-21.4%; RI, 11.3-16%). Conclusion: ICG-SLNB could be an additional or an alternative method for axillary node mapping in breast cancer.<br /> https://journal.waocp.org/article_89063_c054c245e656a6f20b55fe512bfc80a6.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Association of IL-12B rs3212227 and IL-6 rs1800795 Polymorphisms with Susceptibility to Cervical Cancer: A Systematic Review and Meta-Analysis119712068905410.31557/APJCP.2020.21.5.1197ENMojganKarimi-ZarchiDepartment of Obstetrics and Gynecology, Iran University of Medical Sciences, Tehran, Iran.Endometriosis Research Center,
Iran University of Medical Sciences, Tehran, IranHajarAbbasiDepartment of Obstetrics and Gynecology, Shahid Beheshti University of
Medical Sciences, Tehran, Iran.AtiyehJavaheriDepartment of Obstetrics and Gynecology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.AmanehHadadanDepartment of Obstetrics and Gynecology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.BahareMeibodiDepartment of Obstetrics and Gynecology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Razieh SadatTabatabaeiDepartment of Obstetrics and Gynecology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.YaserGhelmaniDepartment of Internal Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Clinical Research
Development Center of Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.HosseinNeamatzadehDepartment of
Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Mother and Newborn Health Research Center,
Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Journal Article20191111Background: Primary studies have shown that the IL-12B rs3212227 and IL-6 rs1800795 polymorphisms are associated with an increased risk of cervical cancer. However, conflicting results warrant a meta-analysis to obtain more precise estimates. Methods: A comprehensive literate search on PubMed, Web of Science, Scopus, CNKI, and SciELO was performed to collect all eligible studies up to November 10, 2019. The pooled odds ratios (OR) and 95% confidence intervals (CI) were used to calculate the risk. This meta-analysis was carried out by utilizing CMA software. Results: A total of eleven case-control studies including four studies on IL-12B rs3212227 and seven studies on IL-6 rs1800795 were selected. Pooled ORs revealed that the IL-6 rs1800795 polymorphism was significantly associated with an increased risk of cervical cancer (C vs. G: OR = 1.294, 95% CI 1.071-1.564, p= 0.007; CC vs. GG: OR = 1.633, 95% CI 1.059-2.520, p= 0.027; CC+CG vs. GG: OR = 1.312, 95% CI 1.048-1.643, p= 0.018; and CC vs. CG+GG: OR = 1.592, 95% CI 1.268-1.999, p≤0.001), but not IL-12B rs3212227 polymorphism. Stratified analysis by ethnicity revealed that both IL-12B rs3212227 and IL-6 rs1800795 polymorphisms were associated with risk of cervical cancer in Asian women. Conclusions: Our pooled data revealed that the IL-12B rs3212227 and IL-6 rs1800795 polymorphisms may be used to identify individuals at high risk of cervical cancer in Asian women.<br /> https://journal.waocp.org/article_89054_f459924c1b1752ff0ff7a23f2b7cbf02.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501An Asian Body to Tackle Cancers in Asia – The Asian National Cancer Centers Alliance120712128909010.31557/APJCP.2020.21.5.1207ENLaurelineGatellierNational Cancer Center, Japan, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.0000-0002-0331-3235TomohiroMatsudaNational Cancer Center, Japan, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.KanagaSabapathyNational Cancer Centre Singapore, 11, Hospital Crescent, Singapore.MinDaiNational Cancer Center, China, 17 Panjiayuan Nanli, Chaoyang District, Beijing 100021,
China.Luh Komang MelaDewiDharmais Hospital - National Cancer Center, Jalan Letjend S. Parman No.84-89 Kecamatan Palmerah, Kota Jakarta
Barat, DKI Jakarta, 11420, Indonesia.Tran ThanhHuongNational Cancer Institute & Hanoi Medical University, Hanoi, Vietnam.KardinahKardinahDharmais Hospital - National Cancer Center, Jalan Letjend S. Parman No.84-89 Kecamatan Palmerah, Kota Jakarta
Barat, DKI Jakarta, 11420, Indonesia.0000-0002-9836-2119Tran VanThuanNational Cancer Institute & Hanoi Medical University, Hanoi, Vietnam.Jong BaeParkNational Cancer
Center of Korea, 323 Ilsan-ro, Ilsandong-gu, Goyang-si Gyeonggi-do, 10408, Republic of Korea.JieHeNational Cancer Center, China, 17 Panjiayuan Nanli, Chaoyang District, Beijing 100021,
China.ErdenekhuuNansalmaaNational Cancer Center of
Mongolia, Nam Yan Ju Street, 13370 Ulaanbaatar, Mongolia.BayarsaikhanLuvsandorjNational Cancer Center of
Mongolia, Nam Yan Ju Street, 13370 Ulaanbaatar, Mongolia.William Ying KheeHwangNational Cancer Centre Singapore, 11,
Hospital Crescent, Singapore.0000-0003-0143-6263ManjuSengarTata Memorial Hospital, Dr. E, Dr Ernest Borges Rd, Parel,
Mumbai, Maharashtra 400012, India.CSPrameshTata Memorial Hospital, Dr. E, Dr Ernest Borges Rd, Parel,
Mumbai, Maharashtra 400012, India.TatsuyaSuzukiNational Cancer Center, Japan, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.Journal Article20200415The socioeconomic burden of cancer is growing rapidly in the Asian region, with a concentrated burden on low- and middle- income countries. The residents of this region, representing almost 60% of the global population, demonstrate an eclectic and complex nature, with huge disparities in ethnicity, sociocultural practices among others. The Asian National Cancer Centers Alliance (ANCCA) was established in 2005 by heads of several national cancer centers (NCCs) in the region to address common issues and concerns among Asian countries. During the first 13 years of ANCCA’s existence, the participating NCCs’ senior managers paved the way toward collaboration through transparent sharing of key facts and activities. Concrete achievements of the Alliance include the Asia Tobacco-Free Declaration, the establishment of the ANCCA Constitution in 2014 as well as the creation of an official website more recently. In November 2019, the most active ANCCA members (China, India, Indonesia, Japan, Korea, Mongolia, Singapore, Thailand, and Vietnam) strengthened the bonds of the entity with the clear aim to halt the increase in cancer and mortality rates in Asian countries by 2030. New opportunities including accelerated cooperation between members as well as collaboration with external and multidisciplinary stakeholders at local, regional and international levels are an essential step to most effectively tackle cancers in Asia.https://journal.waocp.org/article_89090_14de86773629c83a256a159e99738cae.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501The Effect of (E)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-one on MicroRNA-18a, Dicer1, and MMP-9 Expressions against DMBA-Induced Breast Cancer121312198907410.31557/APJCP.2020.21.5.1213ENIda Ayu IkaWahyuniariDepartment of Histology, Faculty of Medicine, Udayana University, Bali, Indonesia.0000-0003-3141-6247I Gusti Kamasan NyomanArijanaDepartment of Histology, Faculty of Medicine, Udayana University, Bali, Indonesia.Ni PutuSriwidyaniDepartment of Anatomical Pathology,
Faculty of Medicine, Udayana University, Bali, Indonesia.HerySuwitoDepartment of Chemistry, Faculty of Science and Technology,
Universitas Airlangga, Surabaya, Indonesia.SitarinaWidyariniDepartment of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada,
Yogyakarta, Indonesia.MuhammadGhufronDepartment of Histology and Cell Biology,Universitas Gadjah Mada, Yogyakarta, Indonesia.MustofaMustofaDepartment
of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.0000000192519851Sofia MubarikaHaryanaDepartment of Histology and Cell Biology,Universitas Gadjah Mada, Yogyakarta, Indonesia.0000-0001-7205-652XJournal Article20180301Background: Most of breast cancer patients are estrogen receptor alpha-positive and have high resistance and side effect of chemotherapeutic drug. Therefore, discovering an effective anticancer agent is needed. This research explored the effect of (E)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-one (APE) on miR-18a, Dicer1, and MMP-9 expressions. Methods: Twenty four female Sprague-Dawley rats were invetigated in this study. The rats were divided into 6 groups of 4. G1 was considered as normal rat. G2, G3, T1, T2, and T3 were given DMBA 20 mg/kgBW twice a week for 5 weeks to induce mammary cancer. After being affiliated with cancer, G2 was given vehicle and G3 was treated with tamoxifen. T1, T2, and T3 were treated with APE intraperitoneally everyday for 21 days at doses of 5, 15, and 45 mg/kgBW/day, respectively. Blood plasma was collected to measure miR-18a expression using qRT-PCR. Mammary tissues were also collected to determine Dicer1 and MMP-9 expressions by using immunohistochemistry. Results: The results showed significant down-regulation of miR-18a relative expression and up-regulation of Dicer1 expression in G3 and T1 compared to G2 (P<0.05). MMP-9 expression has significant decrease in T1 compared to G2 (P<0.05). Conclusion: APE can decrease miR-18a and MMP-9 expressions and increase Dicer1 expression in rat mammary cancer. Therefore, this compound could be a candidate of novel anticancer.https://journal.waocp.org/article_89074_29f072a31493b61f1c4a68b7b7220737.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Association of EGLN2 rs10680577 Polymorphism with the Risk and Clinicopathological Features of Patients with Prostate Cancer122112268905710.31557/APJCP.2020.21.5.1221ENNahidRahimiDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.MahsaAziziDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.GholamrezaBahariChildren and
Adolescent Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.BehzadNarouieUrology and Nephrology Research
Center, Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran,
Iran.MohammadHashemiDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.Genetics of Non-communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.Journal Article20190125Several studies have evaluated the association between EGLN2 4-bp insertion/deletion (ins/del) polymorphism (rs10680577) and many cancers. However, up to date, no study has inspected the impact of rs10680577 polymorphism on prostate cancer (PCa) risk. This case-control study was achieved on 170 pathologically confirmed PCa patients and 196 cancer free men to inspect whether rs10680577 variant is related to the risk and clinicopathological features of patients with PCa. Genotyping was performed by mismatched polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The findings did not support an association between the variant with the risk and clinicopathological characteristics of PCa patients. When we pooled our results with six preceding studies, the findings suggested that rs10680577 variant significantly augmented the risk of overall cancer in heterozygous (OR=1.38, 95 % CI=1.26-1.52, p<0.00001, ins/del vs ins/ins), homozygous (OR=1.66, 95 % CI=1.05-2.61, p=0.029, del/del vs ins/ins), codominant (OR=1.44, 95%CI=1.32-1.58, p<0.00001, ins/del+del/del vs ins/ins), and allele (OR=1.32, 95%CI=1.18-1.49, p<0.00001, del vs ins) genetic models. Additional well designed studies with larger sample sizes are necessary to confirm our findings.https://journal.waocp.org/article_89057_d495c18c61090a4d9facce6c0ecf2dc1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501The Impact of Surgery and Radiotherapy on Health-Related Quality of Life of Individuals with Oral and Oropharyngeal Carcinoma and Short-Term Follow up after Treatment122712348905310.31557/APJCP.2020.21.5.1227ENMarcelo CoelhoGoiatoOral Oncology Center and Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School
of Dentistry, Araçatuba, SP, Brazil.Andressa PaschoalAmorosoOral Oncology Center and Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School
of Dentistry, Araçatuba, SP, Brazil.BrunaSilvaOral Oncology Center and Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School
of Dentistry, Araçatuba, SP, Brazil.Emerson GomesDos SantosDepartment of Business, Paulista School of Politics, Economics and Business, Universidade
Federal de São Paulo (UNIFESP), Osasco, Brazil.Fernanda Pereira DeCaxiasOral Oncology Center and Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School
of Dentistry, Araçatuba, SP, Brazil.Sandro BassoBitencourtOral Oncology Center and Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School
of Dentistry, Araçatuba, SP, Brazil.0000-0001-9140-7516AmaliaMorenoDepartment of Oral Surgery, Pathology and Clinical Dentistry, School of
Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.Daniela MichelineDos SantosOral Oncology Center and Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School
of Dentistry, Araçatuba, SP, Brazil.Journal Article20190201Objective: This study aimed to evaluate the quality of life of patients with oral or oropharyngeal cancer by using specific questionnaires (QLQ-C30 and QLQ-HandN35), varying according to the location of the tumor (oral cavity or oropharynx) and the treatment performed (only surgery or surgery associated with radiotherapy). Methods: Fifty patients were enrolled in this study and answered the EORTC QLQ-C30 and EORTC HandN35 questionnaires, before (baseline), at 1 week, and 3 months after treatment. Internal consistency reliability was calculated with the Cronbach coefficient. The Kruskal-Wallis and Wilcoxon tests were applied and P https://journal.waocp.org/article_89053_a8e8ed59827614415fdc01b3e29f22c1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Genomic Instability in Peripheral Blood and Buccal Mucosal Cells of Marijuana Smokers: The Impact of Tobacco Smoke123512398906410.31557/APJCP.2020.21.5.1235ENDaniel Vitor DeSouzaDepartment of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.Samuel RangelClaudioDepartment of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.Camila Lima FeitosaDa SilvaUniversity São Judas Tadeu, Campus
UNIMONTE, Santos, SP, Brazil.Kevin PereiraMarangoniUniversity São Judas Tadeu, Campus
UNIMONTE, Santos, SP, Brazil.Rogerio CorreaPeresUniversity São Judas Tadeu, Campus
UNIMONTE, Santos, SP, Brazil.Daniel ArakiRibeiroDepartment of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.0000-0001-5057-4983Journal Article20190226Background: The aim of this study was to evaluate cytotoxic, mutagenic and genotoxic effects on buccal mucosa and peripheral blood cells from marijuana and tobacco smokers. Methods: For this purpose, a total of 45 volunteers were distributed into four groups: CTRL group (control): individuals who did not smoke marijuana or tobacco (n = 11); Group M: Marijuana smokers (n = 13); Group T: Tobacco smokers (n = 13); Group M + T: Smokers of both marijuana and tobacco (n = 08). Results: Smokers of both marijuana and tobacco led an increase of micronucleated cells on buccal mucosa when compared to control group. The occurrence of karyolysis showed significant changes in this group as well. The comet assay data revealed genetic damage in peripheral blood cells for all groups of smokers. Conclusion: In summary, our results showed that marijuana and /or tobacco are able to induce genetic damage and cytotoxicity in oral and peripheral blood cells.https://journal.waocp.org/article_89064_bd8c0f70afd857b8b139308b9017eed0.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Exploring Two Protocols of FISH Using Cytocell SYT-SSX Probe on Formalin Fixed Paraffin Embedded Tissue Sections124112458908110.31557/APJCP.2020.21.5.1241ENAidy IrmanYajidDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kota Bharu, Kelantan,
Malaysia.Department of Pathology, Hospital Universiti Sains Malaysia, Kota Bharu, Kelantan, Malaysia.0000-0001-5058-2051Siti NorasikinMohd NafiDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kota Bharu, Kelantan,
Malaysia.Department of Pathology, Hospital Universiti Sains Malaysia, Kota Bharu, Kelantan, Malaysia.0000-0002-0642-0909Nor AziahSalehanDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kota Bharu, Kelantan,
Malaysia.Department of
Pathology, Hospital Queen Elizabeth, Kota Kinabalu, Sabah, Malaysia.Sharifah EmiliaTuan SharifDepartment of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kota Bharu, Kelantan,
Malaysia.Department of Pathology, Hospital Universiti Sains Malaysia, Kota Bharu, Kelantan, Malaysia.0000-0002-0343-4205Journal Article20190326Background: Chromosomal translocation t(X;18)(p11.2;q11.2) is the cytogenetic hallmark of synovial sarcoma and have been identified as an alternative diagnostic strategy in differentiating synovial sarcoma from other histologic mimics. This study was carried out to test the efficacy of two FISH protocols using the SYT-SSX break apart probe from Cytocell. Methodology: Representative paraffin blocks of synovial sarcoma were utilized in this study. FISH study was performed on formalin-fixed paraffin embedded tissue sections using the SYT-SSX break apart probe from Cytocell, to detect two form of SYT-SSX transcript, SYT-SSX1 and SYT-SSX2. FISH protocol, including the hybridization was done following two different protocols, Cytocell FISH protocol and Optimized Dako FISH protocol. Results: Tissue samples subjected to FISH using Cytocell FISH protocol showed the absence of signal corresponding to the probe used. Utilizing Optimized Dako FISH protocol, the two signals (red and green) corresponding to the break-apart probes was detected. These findings suggested that Optimised Dako FISH protocol is more suited for use with the tested probe on paraffin embedded tissues in comparison to Cytocell FISH protocol. Conclusion: Optimised Dako FISH protocol was noted to be more suited for detecting SYT-SSX FISH signals on paraffin embedded tissues in comparison to Cytocell FISH protocol.https://journal.waocp.org/article_89081_619ec076da47f782e54666f04cf54324.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Inhibitory Effect of Eco-Friendly Naturally Synthesized Silver Nanoparticles from the Leaf Extract of Medicinal Detarium microcarpum Plant on Pancreatic and Cervical Cancer Cells124712528908310.31557/APJCP.2020.21.5.1247ENIsmail AbiolaAdebayoIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas,
Malaysia.Microbiology and Immunology Department, School of Biomedical Sciences, Kampala International University, Western
Campus, Ishaka-Bushenyi, Uganda.HasniArsadIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas,
Malaysia.0000-0002-3188-8052Haladu AliGagmanSchool of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang,
Malaysia.Department of Biological Sciences, Faculty of Sciences, Bauchi State University Gadau, 751 Itas Gadau, Nigeria.Noor ZafirahIsmailIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200 Bertam, Kepala Batas,
Malaysia.Mohammed RazipSamianSchool of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang,
Malaysia.Journal Article20190419Background: Recently, nanoparticle synthesis by eco-friendly methods has received tremendous attention due to the method advantages and also because of the application of the nanoparticles in cancer research. Therefore, in this study, we synthesized silver nanoparticles from Detarium microcarpum leaf phytochemicals and evaluated its inhibitory effect on pancreatic and cervical cancer cells. Materials and Methods: Silver nanoparticles (dAgNps) were synthesized by reacting phytochemicals of D. microcarpum leaves with silver nitrate for 12 hours. Cell viability assay was carried out to investigate the cytotoxic effect of dAgNps on HeLa and PANC-1 cells. Results: Scanning electron microscopy (SEM) and transmission electron microscopy(TEM) results revealed the average sizes of dAgNps are 81 nm and 84 nm respectively. The x-ray diffraction (XRD) pattern of dAgNps was similar to that of face centered cubic(fcc) structure of silver as reported by joint committee on powder diffraction standards (JCPDS) and fourier-transform infrared spectroscopy (FTIR) analysis showed that some phytochemicals of D. microcarpum such as polyphenols and flavonoids were likely involved in the reduction of Ag+ to form nanoparticles. Finally, cell viability assay revealed dAgNps inhibited PANC-1 and HeLa cell proliferations with IC50 values of 84 and 31.5 µg/ml respectively. Conclusion: In conclusion, the synthesized nanoparticles from D. microcarpum leaves (dAgNps) have inhibitory effect on pancreatic and cervical cancer cells.https://journal.waocp.org/article_89083_7de45c18da18525922f2871558760afc.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Perceived Deterrence Towards Colonoscopy for Colorectal Cancer Screening among Northern Malaysia Population: A Qualitative Study125312588908810.31557/APJCP.2020.21.5.1253ENMohd AzriMohd SuanClinical Research Center, Hospital Sultanah Bahiyah, Alor Setar, Kedah, Malaysia.0000-0002-9508-7297Wei LeongTanKedah State Health Department, Alor etar, Kedah,
Malaysia.IbtisamIsmailClinical Research Center, Hospital Sultanah Bahiyah, Alor Setar, Kedah, Malaysia.0000-0003-1697-710XMuhammad RadziAbu HassanClinical Research Center, Hospital Sultanah Bahiyah, Alor Setar, Kedah, Malaysia.3Medical Department, Hospital Sultanah Bahiyah, Alor Setar, Kedah, Malaysia.0000-0002-7971-1643Journal Article20190508Background: Patients with positive immunochemical faecal occult blood test results were found to have poor compliance for a subsequent colonoscopy procedure. This study was conducted to explore patients’ perceived deterrence for colonoscopy following a positive stool test. Methods: Using qualitative study method, a phone interview was conducted with 16 patients to elicit their views on the reasons for failure to attend the colonoscopy procedure following a positive stool test. The interviews were audio recorded, transcribed verbatim and translated before proceeded with the data analysis. Content analysis was made on the translated interview, followed by systematic classification of data by major themes. Results: Reasons for nonattendance were categorized under five main themes; unnecessary test, fear of the procedure, logistic obstacles (subthemes; time constraint, transportation problem), social influences, and having other health priority. Lacking in information about the procedure during the referral process was identified to cause misperception and unnecessary worry towards colonoscopy. Fear of the procedure was commonly cited by female respondents while logistic issues pertaining to time constraint were raised by working respondents. Conclusions: More effective communication between patients and health care providers are warranted to avoid misconception regarding colonoscopy procedure. Support from primary care doctors, customer-friendly appointment system, use of educational aids and better involvement from family members were among the strategies to increase colonoscopy compliance.https://journal.waocp.org/article_89088_ec5539b0e5f5975240813d1815ba80b4.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Cytotoxic and Antimetastatic Activity of Hesperetin and Doxorubicin Combination Toward Her2 Expressing Breast Cancer Cells125912678908410.31557/APJCP.2020.21.5.1259ENIka PutriNurhayatiCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.Department of
Pharmacy, Faculty of Medicine, Universitas Brawijaya, Indonesia.AnnisaKhumairaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.Gagas Pradani NurIlmawatiCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.EdyMeiyantoCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.Department of Pharmaceutical Chemistry, Faculty of
Pharmacy. Universitas Gadjah Mada, Indonesia.0000-0002-0886-6322AdamHermawanCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.Department of Pharmaceutical Chemistry, Faculty of
Pharmacy. Universitas Gadjah Mada, Indonesia.0000-0001-5851-3691Journal Article20190522Objective: This study aimed to explore Hesperetin (Hst) potency as a co-chemotherapeutics agent combined with Doxorubicin (Dox), particularly cytotoxic and antimetastasis effects toward MCF-7/HER2 cells. Methods: The cytotoxic effects were measured under MTT assay. The flowcytometry analysis was used to examine the cell cycle modulation and apoptosis evidence, while the effect of migration was assayed by scratch wound healing assay. Western blotting and gelatin zymography were carried out to examine the expression level of proteins, HER2, and Rac1. Results: Under MTT assay, Hst and Dox exhibited to decrease cell viability in a dose-dependent manner with the IC50 value of 377 and 0,8 µM, respectively. The combination of Hst and Dox at the respective doses of 95 and 0,2 µM showed a synergistic effect with the combination index of 0,63. Flow cytometry analysis of Hst-Dox revealed that those compounds caused cell cycle arrest at the G2/M phase and induced apoptosis. Hst also decreased HER2 and Rac1 expression, as shown by western blot. Hst inhibited lamellipodia formation and cell migration, as indicated by microscopic observation and wound healing scratch assay. The antimetastatic activity of Hst was associated with the reduction of Rac1 and MMP9 expression as measured by gelatine zymography assay. Conclusion: These results indicated that the combination of Hst and Dox-induced cell cycle arrest, apoptosis, decreased HER2, Rac1, MMP9 expression, and cell migration. Thus, Hst may have the potential to be developed as a co-chemotherapeutic agent combined with doxorubicin toward HER2 overexpressing breast cancer cells.https://journal.waocp.org/article_89084_b05c654a0a3327566b206a3b931cd56a.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Reversal Effect of Dihydromyricetin on Multiple Drug Resistance in SGC7901/5-FU Cells126912748908610.31557/APJCP.2020.21.5.1269ENMingcaiWuDepartment of Biochemistry, Wannan Medical College, Wuhu, Anhui, P.R.China.Anhui Province Key Laboratory of Active Biological
Macromolecules, Wuhu, Anhui, P.R.China.MingJiangWuhu second Sanatorium for Retired Cadres, Anhui military area, Wuhu, Anhui,
P.R. China.TingDongEncephalopathy Center, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, P.R.
China.LeiXuDepartment of Biochemistry, Wannan Medical College, Wuhu, Anhui, P.R.China.Anhui Province Key Laboratory of Active Biological
Macromolecules, Wuhu, Anhui, P.R.China.JunLvDepartment of Biochemistry, Wannan Medical College, Wuhu, Anhui, P.R.China.Anhui Province Key Laboratory of Active Biological
Macromolecules, Wuhu, Anhui, P.R.China.MengyaXueDepartment of Biochemistry, Wannan Medical College, Wuhu, Anhui, P.R.China.MengzhuHuangDepartment of Biochemistry, Wannan Medical College, Wuhu, Anhui, P.R.China.Journal Article20190621Background: One of the most common treatment for gastric cancer is chemotherapy, however, multiple drug resistance (MDR) induce the therapeutic effect which result in the failure of anticancer therapy. Dihydromyricetin (DMY) was reported to have antitumor activities on various human cancer cells in vitro, our previous studies demonstrated that DMY combined with mitomycin has inhibitory effect on proliferation of gastric carcinoma cells. However, the underlying role of DMY reversing the MDR of gastric carcinoma is poor understood. The aim of this study was to evaluate the reversal effect of DMY on MDR and investigate the molecular mechanisms in vitro. Methods: Using MTT assay, we identified the toxicity of DMY on SGC7901 and SGC7901/5-FU cells. The effect of DMY on 5-FU induced apoptosis was evaluated by flow cytometry analysis. Using RT-PCR and Western blot, we determined the MDR1 mRNA and protein expression. Results: DMY induced growth inhibition in both SGC7901 and SGC7901/5-FU cells, the IC50 value was 13.64±1.15 µg/mL, 20.69±1.82 µg/mL respectively. DMY treatment sensitized SGC7901/5-FU cells to cytotoxicity of 5-FU. The combination of DMY with 5-FU increased the apoptosis rate (9.91%, 16.67%) comparing with 5-FU alone (5.25%). Comparing with the control group, the MDR1 mRNA and protein expression in SGC7901/5-FU cells after treatment of DMY decreased significantly (P< 0.05). Conclusion: In brief, our study demonstrated that DMY effectively reversed multi-drug resistance occurring in SGC7901/5-FU cells cultured in vitro, and the potential mechanism was involved in the downregulation of the MDR1 expression.https://journal.waocp.org/article_89086_964abd40de4245c8cf0b5c9c81d678f7.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Ethanol Extract of Hizikia fusiforme Induces Apoptosis in B16F10 Mouse Melanoma Cells through ROS-Dependent Inhibition of the PI3K/Akt Signaling Pathway127512828908510.31557/APJCP.2020.21.5.1275ENCheolParkDepartment of Molecular Biology, College of Natural Sciences, Dong-eui University, Busan 47340, Republic of Korea.HyesookLeeAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.HyunHwangboAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.Seon YeongJiAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.Min YeongKimAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.So YoungKimAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.Su HyunHongAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.Gi-YoungKimLaboratory of Immunobiology, Department of Marine
Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.Yung HyunChoiAnti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.Department of Biochemistry, College of
Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.0000-0002-1454-3124Journal Article20190722Background: Previous studies have reported that Hizikia fusiforme, an edible brown seaweed, has diverse health-promoting effects; however, evidence for its anti-cancer potential is still lacking. In this study, we examined the effect of ethanol extract of H. fusiforme (EHF) on the proliferation of B16F10 mouse melanoma cells. Methods: Analyses of cell viability and apoptosis were performed to study the actions of EHF on B16F10 cells. Cellular reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) were measured using a flow cytometer. Western blot analysis was carried out to measure apoptosis and phosphoinositide 3-kinase (PI3K)/Akt signaling related proteins. Results: EHF treatment significantly decreased B16F10 cell viability, which was associated with induction of apoptosis. EHF activated caspase-8 and caspase-9, which are involved in the initiation of extrinsic and intrinsic apoptosis pathways, respectively, and also increased caspase-3 activity, a typical effect caspase, subsequently leading to poly (ADP-ribose) polymerase cleavage. In addition, EHF destroyed the integrity of mitochondria and increased Bax/Bcl-2 ratio, which contributed to cytosolic release of cytochrome c. EHF further enhanced intracellular levels of ROS and the addition of N-acetyl cysteine (NAC), a ROS inhibitor, significantly diminished EHF-induced mitochondrial dysfunction and growth inhibition. Moreover, EHF inactivated the PI3K/Akt signaling pathway and LY294002, a PI3K/Akt inhibitor, increased the apoptosis-inducing effect of EHF. However, increased apoptosis and reduced cell viability by simultaneous treatment of EHF and LY294002 were significantly attenuated in the presence of NAC. Conclusion: These results indicate that EHF induces apoptosis through activation of extrinsic and intrinsic apoptotic pathways and ROS-dependent inactivation of PI3K/Akt signaling in B16F10 cells.<br /> https://journal.waocp.org/article_89085_cc4fc138463921d1d84e459dda6d5016.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Promoter Hypermethylation of LATS2 Gene in Oral Squamous Cell Carcinoma (OSCC) Among North Indian Population.128312878905810.31557/APJCP.2020.21.5.1283ENHarshGoelDepartment of Biotechnology, Institute of Applied Medicines and Research Ghaziabad, Uttar Pradesh, India.0000-0003-3904-7343SaloniSinghalDepartment of Biotechnology, Institute of Applied Medicines and Research Ghaziabad, Uttar Pradesh, India.RunjhunMathurDr. A.P.J. Abdul Kalam
Technical University, Lucknow, Uttar Pradesh, India.SaimaSyedaDepartment of Zoology, Delhi University, India.Rishi KumarGuptaSh. Jagannath Charitable
Cancer Hospital, Ghaziabad, Uttar Pradesh, India.AnshumanKumarDharamshila Cancer Hospital and Research Centre, New Delhi, India.AnjuShrivastavaDepartment of Zoology, Delhi University, India.Abhimanyu KumarJhaDepartment of Biotechnology, Institute of Applied Medicines and Research Ghaziabad, Uttar Pradesh, India.0000-0002-6798-2825Journal Article20190724ABSTRACT<br />Large Tumor Suppressor (LATS2) gene are Tumor Suppressor gene, linked with epigenetic modifications. LATS2 promoter hypermethylation is an important epigenetic silencing mechanism leading to cancer. Cancer is the most common, vicious and dangerously increasing diseases of the world today, associated with high morbidity and mortality. Oral cancers (OC) are the blazing universal dilemma and is the sixth most frequent cancer observed in Indian population. Tobacco consumption is the main cause of the increase in OSCC. The association between LATS2 in the pathogenesis of cancers propose that their combination might be studied as a possible molecular marker for particular subgroups of patients. Therefore, the present study tried to investigate whether LATS2 promoter methylation was associated with oral squamous cell carcinoma (OSCC) in North Indian subjects. DNA methylation quantitative studies of LATS2 Tumor Suppressor genes were performed by methylation-specific polymerase chain reaction (MSP). 38 out of 70 patients (55 %) were found to be methylated for LATS2 gene, a statistically significant result was obtained (p-value < 0.005) for LATS2 genes. The results suggest that epigenetic changes may be related to the down-regulation of LATS2 expression. It can be concluded that LATS2 gene plays a significant role in the diagnosis of cancer and provide a better alternative as a diagnostic biomarker. Our data infer that a low LATS2 expression due to methylation may contribute to the cancer progression and could be useful for the diagnosis of OSCC. Therefore, investigation of promoter methylation in such genes may provide a biomarker which may prove to be useful in early detection of Oral Cancer.<br />Keywords: Oral squamous cell carcinoma (OSCC), Epigenetic changes, LATS2 gene, Promoter Hypermethylation, Methylation-Specific PCR (MSP), Biomarkers<br />Abbreviations: OSCC- Oral Squamous Cell Carcinoma; DNA- Deoxyribonucleic acid; LATS-Large Tumor Suppressor (gene); MSP-Methylation-Specific Polymerase Chain Reactionhttps://journal.waocp.org/article_89058_f30fc799f59fa1e35d49d2a7f1538af5.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Modulatory Role of Single Nucleotide Polymorphisms of Distinct Genetic Pathways on Clinical Behavior of Medullary Thyroid Carcinoma128912938908910.31557/APJCP.2020.21.5.1289ENVasudhaMishraSarin Lab, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai,
India.Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400085, India.PradnyaKowtalSarin Lab, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai,
India.Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400085, India.PallaviRane3Clinical Research
Centre (CRC), Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai, India.RajivSarinSarin Lab, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai,
India.Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400085, India.Cancer Genetics Clinic, Tata Memorial Hospital, Tata Memorial Centre, Parel, Mumbai,400012, India.0000000264058282Journal Article20190806Background: Role of RET proto-oncogene as predisposing gene for Medullary Thyroid Carcinoma is well established which provides the basis for clinical management of patients. However clinical behavior of MTC varies considerably among patients. Several studies have investigated whether SNPs in low penetrance genes could modulate the clinical behavior of MTC but with conflicting or inconclusive results. The present study aimed to investigate the modifier effect of 13 SNPs of three distinct genetic pathways -Detoxification, Cell cycle regulation and RET on the clinico-pathological features of hereditary and sporadic MTC. Methods: SNPs were genotyped using RFLP or TaqMan method. The genotypes were correlated with various clinico-pathological parameters (age and calcitonin levels at MTC diagnosis, tumor volume, nodal and distant metastasis). Results: Nodal metastasis was the only clinico-pathological parameter showing significant association with any SNP. In the hereditary MTC group (n=77), incidence of nodal metastases was significantly higher in wild type allele for Cyp1A1m1, CDKN2A and CDKN2C (p=0.01 for all three). In sporadic MTC group (n=361) CDKN2C wild type allele had higher nodal metastasis (p=0.03). Conclusion: In this largest MTC cohort with comprehensive analysis of modulatory role of 13 most frequently studied SNPs with MTC clinical outcome, we observed a statistically significant association of few SNPs with nodal metastasis. However as these SNPs did not show association with any other clinico-pathological parameters like tumor volume or Calcitonin, they may not be true modifier of MTC. Additional large cohort studies with clinico-pathological details and long-term follow-up are needed to identify genetic modifiers of MTC behavior.https://journal.waocp.org/article_89089_850c9f8ac10c2d12a6d433a7ac9655b8.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Clinical Value of NOTCH1 Mutations Detection among Chronic Lymphocytic Leukemia Patients129513018906910.31557/APJCP.2020.21.5.1295ENSalahArefHematology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Egypt.0000-0002-4822-5204MohamedEl -AgderHematology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Egypt.OsamaSalamaHematology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Egypt.TarekAbouzeidHematology
Unit, Internal Medicine Department, Mansoura University Oncology Center, Mansoura Faculty of Medicine, Mansoura,
Egypt..MohamedSabryHematology Unit, Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Egypt.Journal Article20191018Background: The data about the clinical impact of NOTCH1 mutations among Egyptians B – cell chronic lymphocytic patients is not previously identified. We herein, evaluate the prevalence and the prognostic significance of neurogenic locus notch homolog protein-1 (NOTCH1) mutations in B- cell lymphocytic leukemia (B-CLL). Methods: A cohort of 105 Egyptian B-CLL patients aging from 43 to 86 years. PCR products including NOTCH1 exon 26, 27, and distal part of exon 34 expanding the sequences encoding transcription activation domain (TAD) and a peptide sequence rich in proline (P), glutamic acid (E), serine (S), threonine (T) (PEST domains) were sequenced by direct DNA Sanger sequencing. Results: NOTCH1 mutations were detected in 48/105 of patients (45.7%). Mutations in B-CLL patients are insertions (n=21), point mutations (n=18) and deletions (n=12). NOTCH1 mutations showed significant impact on prognosis of B-CLL patients as they were associated with increased bone marrow lymphocytes, more relapse and high incidence of mortality, shortened overall survival and progression free survival, and lymphocytes doubling time, when compared with NOTCH1 wild type B-CLL patients (P= 0.001; 0,005; 0.042; 0.049; 0.008; 0.049 respectively). Conclusion: NOTCH1 mutations were considered as bad prognostic marker in B-CLL and suggested to be included in risk stratification of B-CLL patients at diagnosis.https://journal.waocp.org/article_89069_746a427b6438d5b45a5ed53fd5c72735.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Occupational Exposure and Risk Assessment of Formaldehyde in the Pathology Departments of Hospitals130313098907910.31557/APJCP.2020.21.5.1303ENElhamYahyaeiDepartment of Occupational Health, School of Health, Guilan University of Medical Sciences, Rasht, Iran.BehzadMajlesiDepartment of Management, West Tehran Branch-Islamic Azad University, Tehran, Iran.MohammadNaimi JoubaniDepartment of Environmental Health Engineering, School of Health, Guilan University of Medical Sciences, Rasht, Iran.YasamanPourbakhshiInstitute of Medical Advanced Technologies, Guilan University of Medical Sciences, Rasht, Iran.SamiraGhiyasiDepartment of Environmental Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran.MehdiJamshidi RastaniDepartment of Occupational Health, School of Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.MahmoudHeidariDepartment of Occupational Health, School of Health, Guilan University of Medical Sciences, Rasht, Iran.Research Center of Health and Environment, Guilan University of Medical Sciences, Rasht, Iran.0000-0002-3554-9664Journal Article20191019Background and Objective: Exposure to formaldehyde has adverse effects on health both acutely and over the long term (e.g., carcinogenicity). The substance is widely used in pathology and histology departments. This study focused on cancer risk of formaldehyde in pathology department of five hospitals in Rasht. Materials and Methods: Sampling and determination of formaldehyde in pathology department were carried out based on the NIOSH method of 3500. The working condition and working environment were investigated and a semi quantitative risk assessment were used to health risk assessment of formaldehyde and The individual lifetime cancer probability, which is defined as the increase in the probability of developing cancer during continuous exposure to an air pollutant were used to assess health risks with formaldehyde. Results: The results showed that the exposure level of all subjects were higher than the Occupational Exposure Limit for 8 hours exposure time of formaldehyde. However, in the five occupational groups, the highest weekly exposure index was observed for the Lab Technicians (0.664 ppm) at Hospital no. 5, which could have been due to more daily working hours at this sampling site and a lack of adequate ventilation. The formaldehyde concentration was in the 0.0192 to 0.326 ppm ranges for five hospital pathology departments. The cancer risk ranged from 9.52×10-5 to 1.53×10-3, and it was greater than the WHO acceptable cancer risk level.Conclusions: The results of the risk assessments can be used for managing the chemical exposure of allocated resources for defining control actions. This process plays an important role in reducing the level of exposure to formaldehyde in pathology departments.<br /> https://journal.waocp.org/article_89079_0e337c79a5f0ac05bcd90a78cda522bd.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Aberrant Expression of miR-103, miR-184, miR-378, miR-497 and miR-506 in Tumor Tissue from Patients with Oral Squamous Cell Carcinoma Regulates the Clinical Picture of the Patients131113158905910.31557/APJCP.2020.21.5.1311ENMaryamGhffariTuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.0000-0002-4806-1086MiladAsadiTuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.0000-0001-7846-2908DariushShanaehbandiTuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.0000-0002-0449-0607SoghraBornehdeliImmunology Research
Center, Tabriz University of Medical Sciences, Tabriz, Iran.0000-0002-5513-4175MahsaSadeghzadehImmunology Research
Center, Tabriz University of Medical Sciences, Tabriz, Iran.0000-0003-2440-0052HaniyeMohammad Reza KhaniImmunology Research
Center, Tabriz University of Medical Sciences, Tabriz, Iran.0000-0002-2217-9940ShahramGhasembaglouImmunology Research
Center, Tabriz University of Medical Sciences, Tabriz, Iran.Medical Faculty, Tabriz University of Medical Science, Tabriz,
Iran.0000-0003-2340-3972Journal Article20191023Background: This study aimed to evaluate the expression patterns of miR-103, miR-184, miR-378, miR497 and in squamous cell carcinoma (SCC) of the tongue and to be compared with normal peripheral tissues. Methods: Tumor and marginal tissues were obtained from 50 patients with OSCC. After RNA extraction, expression level of miR-103, miR-184, miR-378, miR497, and miR506 was estimated using SYBR green master mix and real-time quantitative PCR. Results: It was observed that, there was no detectable difference in expression level of miR-103 between tumoral and marginal tissues. However, expression level of miR-184, and miR-378 showed significant increase in tumor tissue samples compared to marginal tissue samples. MiR-497 and miR-506 demonstrated considerable decrease in tumoral cells in comparison with peripheral tissues. Moreover, the expression level of miRNAs was associated with clinicopathological features of the patients. Conclusions: Our data indicated that miR-184, miR-378, miR-497, and miR-506 can be used as a potential diagnostic and prognostic biomarker in OSCC. Nevertheless, more studies are needed to confirm this claim.<br /> https://journal.waocp.org/article_89059_388db3bc59e655e82c5bdf44b1ed19a3.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Epidemiologic and Health Economic Evaluation of Cervical Cancer Screening in Rural China131713258907710.31557/APJCP.2020.21.5.1317ENFeiZhaoWest China School of Public Health and West Fourth Hospital, Sichuan University, Chengdu, China.YingWenWest China School of Public Health and West Fourth Hospital, Sichuan University, Chengdu, China.Department of Molecular
Epidemiology, Shenzhen Center for Disease Control and Prevention, Shenzhen,China.YangLiNational Cancer Center/National Clinical
Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,
China.Institute of Medical Information, Chinese Academy of Medical Sciences and Peking Union M Medical College, Beijing,
China.SiyuanTaoWest China School of Public Health and West Fourth Hospital, Sichuan University, Chengdu, China.LiMaDalian Medical University, Dalian, China.YuqianZhaoNational Cancer Center/National Clinical
Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,
China.Sichuan Cancer Hospital &Institute, Sichuan Cancer Center, School of
Medicine, University of Electronic Science &Technology of China, Chengdu, China.LeDangNational Cancer Center/National Clinical
Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,
China.YingWangWest China School of Public Health and West Fourth Hospital, Sichuan University, Chengdu, China.FanghuiZhaoNational Cancer Center/National Clinical
Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,
China.JingheLangPeking Union Medical college Hospital,
Chinese Academy of Medical Sciences/Peking Union Medical College(CAMS/PUMC), Beijing, China.YoulinQiaoNational Cancer Center/National Clinical
Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing,
China.Chun-xiaYangWest China School of Public Health and West Fourth Hospital, Sichuan University, Chengdu, China.Journal Article20191026Background: Cervical cancer is preventable and curable by detected early and managed effectively. To explore the most economical and effective cervical cancer screening strategies would lay a solid foundation for reducing the health and economic burden of cervical cancer. Methods: A Markov model was established for a cohort of 100,000 female to simulate the natural history of cervical cancer. 18 screening strategies were estimated including careHPV, Thin prep cytologic (TCT), Visual inspection with acetic acid/ Lugol’s iodine (VIA / VILI), careHPV in series with VIA / VILI, careHPV in series with TCT, three methods parallel connection every 1, 3, 5 years respectively. Model outcomes included cumulative risk of incidence and death of cervical cancer, quality-adjusted life years (QALYs), cost-effectiveness ratios (CERs), incremental cost-effectiveness ratios (ICERs), cost-utility ratios (CURs) and benefits. Results: According to the results of epidemiological analysis, careHPV similar to the parallel connection every 1 year achieved highest epidemiological effects via reducing the cumulative risk of onset and death by more than 98 %. In health-economic terms, CER among all the screening strategies ranged from -756.34 to 113040.3 Yuan per year and CUR ranged from -169.91 to 11968.27 Yuan per QALY. The benefit ranged from -1629 to 996 Yuan. The incremental cost-effectiveness analysis showed that three methods in parallel every 1 year, TCT every 1 year, VIA/VILI every 1, 3, 5 years and careHPV every 5 years were dominant strategies. Conclusion:Considering the economic and health benefits of all the strategies, our results suggested careHPV every 3 or 5 years and VIA/VILI every 1 or 3 years eventually were more appropriate as screening methods in rural China.https://journal.waocp.org/article_89077_0ce42fe55ea206f9d9ba70ddf6e9b354.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Sequential Chemoradiotherapy Compared to Radiotherapy in Endometrial Carcinoma132713328906510.31557/APJCP.2020.21.5.1327ENOmimaElemamOncology Center, King Abdullah Medical City, Makkah, Saudi Arabia.Oncology Center, Mansoura University, Mansoura, Egypt.SehamAbdelkhalekOncology Center, King Abdullah Medical City, Makkah, Saudi Arabia.Department of Radiotherapy , Mansoura University, Mansoura, Egypt.DoaaAbdelmoetyResearch Center, King Abdullah Medical City, Makkah,
Saudi Arabia.EngyAboelnagaDepartment of Radiotherapy , Mansoura University, Mansoura, Egypt.ReemBarakaDepartment of Biology, Colorado State University, Fort Collins, USA.AhmedZeeneldineOncology Center, King Abdullah Medical City, Makkah, Saudi Arabia.NCI, Faculty of Medicine, Cairo University,
Cairo, Egypt.Journal Article20191104Background: The role of combined modality in the adjuvant treatment of Endometrial Cancer has not been established. This study aims to assess the benefits of Sequential Chemoradiotherapy (SCRT) compared to Radiotherapy (RT) alone in the treatment of patients with Endometrial Cancer. Methods: Retrospective analysis of patients with Endometrial Cancer stage I to stage III C at King Abdullah Medical city, Makkah. Each group of patients was assigned to receive External pelvic RT, brachytherapy or both. While a second group received SCRT consisting of six cycles of Carboplatin (AUC 5) and Paclitaxel 175 mg/m2 followed by radiotherapy. Results: Fifty-six women were treated of which 26 received SCRT and 30 received RT. The two groups had a median age of 58 years old ranging from 34 – 84 years old with no other statistically significant difference. Patients who received SCRT had poorer prognostic tumor characteris-tics. Median follow-up was 29.6 months (95% CI: 19.6-39.5 months). All deaths (n=5) were exclusively in the RT group. The 2 and 4-year OS rates were 100% and 100% in SCRT group versus 87.3% and 64.9% in RT group (hazard ratio [HR] 0.018 [95% CI: 0-24.4; p= 0.038); The 2- and 4-year DFS were 100% and 100% in SCRT group versus 78.1% and 43.9% in RT group (HR 0.102 [95% CI: 0.103-0.805; p= 0.008). Conclusion: Adjuvant chemotherapy given before radiotherapy for Endometrial Cancer may lessen the effect of high-risk features on the DFS and OS. Randomized clinical trials are needed to determine the benefits of early Systemic Therapy.https://journal.waocp.org/article_89065_00c3260657381437dd1b3af9881e80e2.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Evaluation of Antibiotic Resistance and Virulence Genes among Clinical Isolates of Pseudomonas aeruginosa from Cancer Patients133313388908010.31557/APJCP.2020.21.5.1333ENNaveedAhmedDepartment of Microbiology, University of Central Punjab Lahore, Pakistan.Department of Microbiology, Pakistan Kidney and
Liver Institute and Research Center, Lahore, Pakistan.0000-0003-1504-1705ZeshanAliCollege of Food Engineering and Nutritional Sciences, Shaanxi Normal University. Xi’an 710119, China.0000-0001-8300-1436MahparaRiazDepartment of Microbiology, Pakistan Kidney and Liver Institute and Research Center, Lahore, Pakistan.BasitZeshanDepartment of Microbiology, University of Central Punjab Lahore, Pakistan.Javed IqbalWattooDepartment of Microbiology, University of Central Punjab Lahore, Pakistan.Muhammad NaveedAslamDepartment of Microbiology, University of Central Punjab Lahore, Pakistan.Journal Article20191122Objectives: The objectives of this study were to evaluate P. Aeruginosa isolates from cancer patients for the phenotypic pattern of antibiotic resistance and to detect the gene responsible for virulence as well as antibiotic resistance. Methods: A total of 227 P. aeruginosa isolates were studied and 11 antibiotics were applied for susceptibility testing. PCR detection of the genes BIC, TEM, IMP, SPM, AIM, KPC, NDM, GIM, VIM, OXA, toxA and oprI was done. Finally, the carbapenem resistant isolates were tested for phenotypic identification of carbapenemase enzyme by Modified Hodge test. Results: The results showed that the isolates were resistant to imipenem (95%), cefipime (93%), meropenem (90%), polymixin B (71%), gentamicin (65%), ciprofloxacin (48%), ceftazidime (40%), levofloxacin (39%), amikacin (32%), tobramycin (28%) and tazobactum (24%). The PCR detection of the carbapenem resistant genes showed 51% isolates were positive for IMP, GIM and VIM, 38% for AIM and SPM, 30% for BIC, 20% for TEM and NDM, 17% for KPC and 15% for OXA. However, toxA and oprI genes were not detected. 154 carbapenem resistant isolates were found positive phenotypically for carbapenemase enzyme identification by Modified Hodge test. Conclusion: The co-existence of multiple drug-resistant bodies and virulent genes has important implications for the treatment of patients. This study provides information about treating drug-resistant P. Aeruginosa and the relationship of virulent genes with phenotypic resistance patterns.<br /> https://journal.waocp.org/article_89080_daf2a303f08184abe3ee0532f70a47ae.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Barriers and Attitudes toward Breast Cancer Screening among Omani Women133913478906210.31557/APJCP.2020.21.5.1339ENMohammedAl-AzriDepartment of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Sultanate of Oman.0000-0002-6136-4081KawtherAl-RubaieDepartment of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Sultanate of Oman.ShamsaAl-GhafriDepartment of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Sultanate of Oman.MustafaAl-HinaiDepartment of Family Medicine and Public Health, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat,
Sultanate of Oman.SathiyaMurthi PanchatcharamResearch Section, Medical Simulation and Skills Development Centre, Oman Medical Specialty Board, Muscat, Sultanate of OmanJournal Article20191202Background: Breast cancer (BC) is the most common cancer accounting for 24.5% of Omani female cancer. Early detection of BC through regular breast cancer screening (BCS) has been found to decrease mortality rates. The aim of this study was to identify knowledge, attitudes and barriers of Omani women toward BCS. Methods: A cross-sectional study was conducted among adult Omani women attending the Sultan Qaboos University Hospital (SQUH). Results: A total of 358 Omani women participated from 420 invited (response rate = 85.2%). Most women (92.1%) thought that BC could be cured if detected early, but less than half (46.8%) thought that they were at risk of BC if a relative had BC. The majority (81.1%) were aware that BCS was available in Oman, though less than half (48.5%) knew where to go for BCS and most (83.8%) had not undergone BCS before. The most common personal-related barriers to BCS were fear of BC diagnosis (40.8%), fear of treatment (52.1%) and embarrassment of the breast examination (46.6%). The most perceived system-related barriers to BCS were the concern over the availability of a male doctor (46.6%) or a non-Arabic speaking doctor (38.7%) and not recommended by the doctor (46.3%). Univariate binary logistic regression showed that attitudes toward BCS were influenced by their previous experiences of BCS (odds ratio [OR] = 2.28; 95% confidence interval [CI]: 1.18-4.41) and their willingness to participate in the future BCS [OR = 1.96; 95% CI: 1.14-3.37). Conclusion: Although Omani women showed an interest toward BCS, few had undergone BCS. Several cultural, practical and personal-related barriers were noted to interfere with BCS in Oman. Addressing these concerns through involving healthcare providers to reassure the women and implementing a national strategy of BCS could improve BCS screenings and early diagnosis of BC.https://journal.waocp.org/article_89062_c339f36377e83cb88867b6a5b178c864.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Structural Recognition and Binding Pattern Analysis of Human Topoisomerase II Alpha with Steroidal Drugs: In Silico Study to Switchover the Cancer Treatment134913558906610.31557/APJCP.2020.21.5.1349ENQazi Mohammad SajidJamalDepartment of Health Informatics, College of Public Health and Health Informatics, Qassim University, Saudi Arabia.0000-0001-5525-708XJournal Article20191204Background and objective: Topoisomerase TOP-IIA (TTOP-IIA) is widely used as a significant target for cancer therapeutics because of its involvement in cell proliferation. Steroidal drugs have been suggested for breast cancer treatment as aromatase enzymes inhibitors . TTOP-IIA inhibitors can be used as a target for the development of new cancer therapeutics. Materials and Methods: In this study, we conducted a docking study on steroidal drugs Anastrozole (ANA), Letrozole (LET), and exemestane (EXE) with TTOP-IIA to explore the therapeutic area of these drugs. Results: The binding interaction of EXE drug had significant docking interaction which is followed by ANA and LET. Thus, all these drugs could be used to inhibit the TTOP-IIA mediated cell proliferation and could be a hope to treat the other types of cancers. Among all three tested steroidal drugs, EXE showed binding energy -7.05 kcal/mol, hydrogen bond length1.78289 Å and amino acid involved in an interaction was A: LYS723:HZ3 -: UNK1:O6. Conclusion: The obtained data showed the most significant binding interaction analyzed with the tested enzyme. Thus, in vitro laboratory experimentation and in vivo research are necessary to put forward therapeutic repositioning of these drugs to establish them as a broad spectrum potential anticancer drugs.<br /> https://journal.waocp.org/article_89066_115ef7a4275cd864c9e0ecb63f88e6b1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Understanding Facilitators and Barriers of Online Cancer Information Utilization among Cancer Survivors and their Families: Focus on the Theory of Planned Behavior135713628907110.31557/APJCP.2020.21.5.1357ENDong-SooShinDivision of Nursing, Research Institute of Nursing Science, Hallym University, Republic of Korea.SaeromKimDepartment of Health
Management and Policy, School of Medicine, Kangwon National University, Republic of Korea.Heui-SugJoDepartment of Health
Management and Policy, School of Medicine, Kangwon National University, Republic of Korea.0000-0003-0245-3583Journal Article20191228Objective: Cancer survivors have various health care needs and are willing to be proactive with their health maintenance. Online information would be a useful resource to guide cancer survivors and their family members. Therefore, identifying the factors that influence Internet searching behaviors among cancer survivors and their family members is a first step toward providing better health care services for cancer care. Methods: We performed focus group interviews that were based on the Theory of Planned Behavior, with thirty-one participants to explore factors related to Internet search behaviors among cancer survivors and their family members. Results: Six themes were identified in the analysis of participant interviews. Attitudes toward searching for health information on the Internet included the themes “Fulfilling unmet needs” and “Confirmation through second opinion.” Themes related to social norms included “a required step for sure” and “helping each other.” In terms of perceived behavioral control, themes included “difficult to choose because of being ‘overwhelmed with information,’” and “complex searching milieu.” Conclusion: It was clear that cancer survivors and their family members had unmet needs for maintaining their health status. They wanted to be informed and actively involved in the decision-making process regarding health management. Consultation and education provided to patients by doctors should not only include information on diet and nutrition but also information on the resulting complications to satisfy their need for reliable health information.https://journal.waocp.org/article_89071_b9fcf029bdef7e831d18185bb9d8e4f4.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Association between Healthy Eating Index-2015 and Breast Cancer Risk: A Case-Control Study136313678906010.31557/APJCP.2020.21.5.1363ENHamedKord- VarkanehStudent Research Committee, Department of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.AmmarSalehi- SahlabadiStudent Research Committee, Department of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.MeysamZarezadeDepartment of Clinical Nutrition, Student Research Committee,
Nutrition Research Center, School of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.JamalRahmaniStudent Research Committee, Department of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Shing ChengTanUKM Medical Molecular Biology Institute, Universiti Kebangsaan
Malaysia, Kuala Lumpur, Malaysia.AzitaHekmatdoostStudent Research Committee, Department of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.BahramRashidkhaniDepartment of Community Nutrition, Faculty of Nutrition Sciences and Food Technology,
Shahid Beheshti University of Medical Sciences, Tehran, Iran.Journal Article20200104Objective: Diet quality is known to influence cancer risk. The Healthy Eating Index (HEI) is one of the most frequently used measures of diet quality. However, the association between HEI-2015 and breast cancer risk is not known. The present study was undertaken to evaluate the association between HEI-2015 and breast cancer risk. Methods: A case-control study comprising 134 breast cancer patients and 265 cancer-free controls were conducted. Dietary intakes were assessed using a validated food frequency questionnaire (FFQ), from which the HEI-2015 score was calculated. Logistic regression was used to derive the odds ratios (ORs) for measuring the association between HEI-2015 scores and breast cancer risk. Results: Subjects in the top quartile of HEI-2015 had a 46% lower chance of breast cancer compared with subjects in the bottom quartile (OR 0.54; 95% CI 0.30, 0.98). After adjustment for potential confounders such as age, age at menarche, oral contraceptive drug use, menopausal status, marital status, body mass index, smoking and education level, the association between HEI-2015 score and a lower risk of breast cancer was enhanced (OR 0.32; 95% CI 0.16, 0.65). Conclusion: We successfully demonstrated that a higher HEI-2015 score was associated with a reduced breast cancer risk.https://journal.waocp.org/article_89060_f6583040281d985695f1541c27282505.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Evaluation of the Biological Efficiency of Silver Nanoparticles Biosynthesized Using Croton tiglium L. Seeds Extract against Azoxymethane Induced Colon Cancer in Rats136913898905210.31557/APJCP.2020.21.5.1369ENWael MahmoudAboulthanaBiochemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt.http://orcid.org/0000-0002-4848-0731Noha El-SayedIbrahimMicrobial Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza,
Egypt.0000-0002-7176-4687Noha MohamedOsmanCell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt.Mohamed MahmoudSeifToxicology and Food contaminants, Food Industry and Nutrition Division, National Research Center, Dokki, Giza, Egypt.Amgad KamalHassanBiochemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt.Ahmed MahmoudYoussefPackaging
Materials Department, National Research Center, Dokki, Giza, Egypt.0000-0002-6501-6658Amal MostafaEl-FekyPharmacognosy Department, Pharmaceutical and Drug
Industries Research Division, National Research Centre, Dokki, Giza, Egypt.0000-0002-4805-3254A AMadboliAnimal Reproduction and Artificial Insemination
Department, Veterinary Division, National Research Centre, Dokki, Giza, Egypt .Journal Article20200109Background: Colorectal cancer (CRC) is considered as the most common type of gastrointestinal cancers. Chemotherapy became limited due to the adverse side effects. Therefore, the most effective Croton tiglium extract was selected to be incorporated by silver nanoparticles (Ag-NPs) then evaluated against colon cancer induced by azoxymethane (AOM) in rats. Methods: Different hematological and biochemical measurements were quantified in addition to markers of oxidative stress. Specific tumor and inflammatory markers were assayed. Colonic tissues were examined histopathologically in addition to immunohistochemistry (IHC). Native proteins and isoenzymes patterns were electrophoretically assayed beside expression of Tumor Protein P53 (TP53) and Adenomatous Polyposis Coli (APC) genes in colonic tissues. Results: It was found that AOM caused significant (P≤0.05) elevation in the hematological and biochemical measurements. C. tiglium nano-extract restored these measurements to normalcy. Tumor and inflammatory markers elevated significantly (P≤0.05) in sera of AOM induced colon cancer group in addition to increasing peroxidation products with decline in antioxidant enzymes activities in colon tissues. Nano-extract restored these measurements to normalcy in post-treated group. Histopathological study revealed that nano-extract minimized severity of inflammatory reactions in all nano-extract treated groups and prevented anti-Keratin 20 antibody expression in post-treated group. The lowest similarity index (SI%) values were noticed with electrophoretic protein (SI=71.43%), lipid (SI=0.00%) and calcium (SI=75.00%) moieties of protein patterns, catalase (SI=85.71%), peroxidase (SI=85.71%), α-esterase (SI=50.00%) and β-esterase (SI=50.00%) isoenzymes in colon cancer group. Furthermore, AOM altered the relative quantities of total native bands. The nano-extract prevented the alterations that occurred qualitatively in nano-extract post-treated group and quantitatively in all nano-extract treated groups. Levels of TP53 and APC gene expression increased in AOM injected group and nano-extract restored their levels to normalcy in the post-treated group. Conclusion: C. tiglium nano-extract exhibited ameliorative effect against the biochemical and molecular alterations induced by AOM in nano-extract post-treated group.https://journal.waocp.org/article_89052_0f10ef8173c2f01bdc1feb7ea59179f0.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501A Prospective Randomized Trial to Compare Safety, Acceptability and Efficacy of Thermal Ablation and Cryotherapy in a Screen and Treat Setting139113988908710.31557/APJCP.2020.21.5.1391ENDipanwitaBanerjeeChittaranjan National Cancer Institute, Kolkata, West Bengal, India.RanajitMandalChittaranjan National Cancer Institute, Kolkata, West Bengal, India.AmitMandalChittaranjan National Cancer Institute, Kolkata, West Bengal, India.IshitaGhoshChittaranjan National Cancer Institute, Kolkata, West Bengal, India.SrabaniMittalChittaranjan National Cancer Institute, Kolkata, West Bengal, India.RichardMuwongeInternational Agency for Research on Cancer, Lyon, France.0000-0002-3448-0503EricLucasInternational Agency for Research on Cancer, Lyon, France.0000-0002-1252-1925ParthaBasuInternational Agency for Research on Cancer, Lyon, France.0000-0003-0124-4050Journal Article20200120Background: The prospective randomized study aimed to compare the safety, acceptability and efficacy of thermal ablation (TA) to that of cryotherapy in screen and treat setting. Methods: The participants were recruited prospectively in a community-based screening clinic in India. Women positive on visual inspection with acetic acid (VIA) test and/or Human Papillomavirus (HPV) test were assessed for eligibility for ablative treatment. Total 286 eligible women were randomized to receive either cryotherapy (N=150) or TA (N=136) performed by health workers. Colposcopy and cervical biopsy were performed on all, prior to treatment. Post-treatment follow-up was after one year with colposcopy and biopsy. Results: Both the treatment methods had high acceptability. Significantly higher proportion of women treated by cryotherapy reported pain compared to women treated by TA, though intensity was mild in vast majority of them. Approximately 30% of women in both arms had histologic abnormalities, mainly CIN 1, and among those who attended follow-up 74.1% and 81.0% didn’t have any CIN after cryotherapy and TA respectively. Conclusion: TA is as acceptable and safe as cryotherapy in screen and treat setting. TA has the logistic advantages for the low-resourced settings as the machines are more portable, do not require costly refrigerant gas and battery-driven models are available. The cure rates for CIN 1+ lesions in our study were comparable between cryotherapy and TA.https://journal.waocp.org/article_89087_982f06944f95e086371e4a5f5ec3f4cf.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Accelerated Radiotherapy with Concurrent Chemotherapy in Locally Advanced Head and Neck Cancers: Evaluation of Response and Compliance139914078905510.31557/APJCP.2020.21.5.1399ENKarimMashhourKasralainy Center of Clinical Oncology and Nuclear Medicine, Cairo University, Cairo, Egypt.0000-0001-9980-6474HishamAtefKasralainy Center of Clinical Oncology and Nuclear Medicine, Cairo University, Cairo, Egypt.AhmedSelimKasralainy Center of Clinical Oncology and Nuclear Medicine, Cairo University, Cairo, Egypt.MostafaA MoezPhysics department at Kasralainy
Center of Clinical Oncology and Nuclear Medicine, Cairo University, Cairo, Egypt.HussamZawamKasralainy Center of Clinical Oncology and Nuclear Medicine, Cairo University, Cairo, Egypt.YasserAbo-MadyanDepartment of Radiation Oncology, University
Medical Center Mannheim, University of Heidelberg, Mannheim, Germany.Journal Article20200121Purpose: Concurrent chemo-radiotherapy (CCRT) is the primary treatment modality for locally advanced head and neck squamous cell cancer patients (LAHNSCC). Intensity modulated radiotherapy (IMRT) with simultaneous integrated boost (SIB) and concurrent chemotherapy is not broadly implicated in our region mainly because of the lack of experience. This study aims at evaluating the response and compliance of this approach in our patients. Methods: Forty patients with LAHNSCC were included and 50% received induction chemotherapy. All the patients were treated with IMRT-SIB radiotherapy for 70Gy over 33 daily fractions. Weekly cisplatin (40mg/m2) was administered during the radiation course.Results: With median follow-up of 1.5 years, LC was achieved in 82.5% of cases and distant control rate was 90%. More than 5 interrupted radiation sessions and GTV volume > 50 cc significantly affected LRC (P= 0.02 and 0.001 respectively). Eighty percent of cases experienced grade 3 or 4 toxicities. Induction chemotherapy and PTV-70 volume >150 cc significantly affected the degree of toxicities (P=0.018 and 0.0001 respectively).The 2 years disease free survival (DFS) was 77%. ECOG PS, large GTV volume (> 50 cc) and RT interruption (>5 sessions) had negative impact on DFS (P= 0.041, 0.002 and 0.001 respectively). The 2 years overall survival (OS) was 87%. Radiation interruption (> 5 sessions) was the only factor which had significant detrimental effect on OS (P= 0.001). Conclusion: Induction chemotherapy seems to have a negative impact on patient’s compliance to CCRT. Bulky tumors and prolonged radiation interruptions were associated with significantly lower LRC, DFS and OS.https://journal.waocp.org/article_89055_3f8fbad9f3679a67a0151d88f4c2f061.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Prevalence and Antibiotic Resistance Patterns of Helicobacter pylori Infection in Koh Kong, Cambodia140914138905110.31557/APJCP.2020.21.5.1409ENNatsudaAumpanGastroenterology Unit, Department of Medicine, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand.Digestive diseases Research Center (DRC), Thammasat University, Pathumthani, Thailand.Ratha-KornVilaichoneGastroenterology Unit, Department of Medicine, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand.Digestive diseases Research Center (DRC), Thammasat University, Pathumthani, Thailand.Department of Medicine, Chulabhorn International College of Medicine (CICM) at Thammasat University, Pathumthani, Thailand.0000-0003-4298-9331PornpenGamnaraiDigestive diseases Research Center (DRC), Thammasat University, Pathumthani, Thailand.Department of Biochemistry, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.LikhasitSanglutongGastroenterology Unit, Department of Medicine, Faculty of Medicine, Thammasat University Hospital, Pathumthani, Thailand.ThaweeRatanachu-ekDepartment of Surgery, Rajavithi Hospital, Bangkok, Thailand.VarochaMahachaiDigestive diseases Research Center (DRC), Thammasat University, Pathumthani, Thailand.Gastrointestinal and Liver Center, Bangkok Medical Center, Bangkok, Thailand.YoshioYamaokaDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan.Journal Article20200122Background: Gastric cancer, which is the leading cause of cancer mortality in Cambodia, can be prevented by Helicobacter pylori (H. pylori) eradication. There is limited data about H. pylori strains in Cambodia. This study aimed to evaluate H. pylori prevalence and antibiotic resistance in Koh Kong, Cambodia. Methods: 118 Cambodian dyspeptic patients were scheduled to enter this study and 58 were enrolled between July and September 2019. All patients underwent upper GI endoscopy. 3 gastric biopsies were obtained for rapid urease test, H. pylori culture with E-test and GenoType® HelicoDr (Hain Lifescience factory, Germany). 3-mL blood sample was collected for CYP2C19 genotyping. Results: 58 subjects were enrolled (40 females, 18 males, mean age 43.8 years). Overall H. pylori prevalence was 31.0%. Antibiotic resistance rates were 78.6% for metronidazole, 50.0% for fluoroquinolones, and 27.8% for clarithromycin. There was no amoxicillin and tetracycline resistance. More than half of H. pylori strains (57.1%) were multidrug-resistant. Most (35.7%) were resistant to metronidazole and quinolone. Poor, intermediate and rapid metabolizers were 5.5%, 38.9% and 55.6%, respectively. Conclusions: H. pylori infection remains common infection in Cambodia. High prevalence of clarithromycin, metronidazole, levofloxacin and multidrug-resistant H. pylori is still major problems in Cambodia. Treatment regimens without clarithromycin and quinolone such as 14-day bismuth-based quadruple therapy might be an appropriate choice for H. pylori eradication in this particular area.https://journal.waocp.org/article_89051_a445cda2b3b1da654c53d92b90a05dea.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Awareness of Breast Cancer among Female Students and Faculty from Najran University, Najran, Saudi Arabia141514228906810.31557/APJCP.2020.21.5.1415ENSaeed AliAlsareiiDepartment of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia.Saad MansourAlqahtaniDepartment of Surgery, College of
Medicine, Majmaah University, Majmaah, Saudi Arabia.Abdulrahman ManaaAlamriDepartment of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia.Hajr HassanAl-WadeiDepartment of Surgery, College of Medicine, Najran University, Najran, Saudi Arabia.Samer AliAl-ammariDepartment of Surgery, King Khalid Hospital, Najran, Saudi Arabia.Awad MohammedAl-QahtaniDepartment of Family and Community Medicine, College of Medicine, Najran University, Najran, Saudi Arabia.AhmedAbu-ZaidCollege of
Medicine, Alfaisal University, Riyadh, Saudi Arabia.College of Graduate Health Sciences, University of Tennessee Health Science
Center, Memphis, Tennessee, United States.0000-0003-2286-2181Journal Article20200122Background: Breast cancer (BC) is the leading malignancy among women in Najran, Saudi Arabia. However, not much is known about the public’s awareness of BC. This study explored the general knowledge, early warning signs, risk factors and sources of information about BC. Methods: An online-based, anonymous, self-rating, cross-sectional and survey-based study was conducted from March-2019 to April-2019. Three-hundred female students and/or faculty from College of Medicine, Najran University (Najran, Saudi Arabia) participated in the study. Results: A total of 232 students (77.3%) and 68 faculty (22.7%) responded to the survey. Our study showed that nulliparity (83.8%) and early menarche before 12 years of age (29.7%) were the most pertinent obstetric risk factors of BC. Conversely, lack of physical activity (66.3%) and family history of BC (18%) were the most substantial non-obstetric risk factors of BC. According to pre-defined criteria, while the surveyed research subjects demonstrated ‘good’ general knowledge about BC (75.3%), they unfavorably exhibited ‘poor’ knowledge about the warning signs of BC (94.3%). The predictors of ‘good’ overall knowledge (general knowledge plus signs knowledge about BC) included age, marital status, educational level and family history (all p<0.05, two-tailed Chi-square test). Apart from the campaigns’ educational materials (43%), the top source of knowledge about BC was internet (33%), whereas the lowest ones were healthcare professionals (11.3%) and training workshops (7.3%). Conclusions: The surveyed research subjects harbored risk factors of BC and demonstrated ‘poor’ knowledge about the warning early signs of BC. We call for rigorous and well-crafted educational campaigns geared toward improving the awareness level of BC among women in Najran province.https://journal.waocp.org/article_89068_3389db077cd3115927b4d439fb60d57d.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Factors that Affected Women Undergoing Cryotherapy Following Cancer Screening with Visual Inspection of the Cervix Using Acetic Acid Method142314298906710.31557/APJCP.2020.21.5.1423ENDahlanNapitupulu1Field Epidemiology Training Program, Master of Public Health Postgraduate Program, Faculty of Medicine, Public Health and
Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.Port Health Office Class II, Jayapura, Papua, Indonesia.HerinditaPuspitaningtyasClinical
Epidemiology Program, Master of Clinical Medicine Postgraduate Program, Faculty of Medicine, Public Health and Nursing,
Universitas Gadjah Mada, Yogyakarta, Indonesia.0000-0003-1929-5905KhabibMualimTemanggung District Health Office, Central Java, Indonesia.ArdhanuKusumantoDivision of
Gynecologic Oncology, Department of Obstetrics and Gynecology, Faculty of Medicine, Public Health and Nursing, Universitas
Gadjah Mada/Dr Sardjito General Hospital, Yogyakarta, Indonesia.LuthfanLazuardiDepartment of Public Health, Faculty of Medicine, Public
Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.Susanna HildaHutajuluDivision of Hematology and Medical Oncology,
Department of Internal Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr Sardjito General
Hospital, Yogyakarta, Indonesia.0000-0001-9236-9435Journal Article20200124Background: The Indonesian government has applied the cancer “see and treat” method which involves a visual inspection using acetic acid (VIA), followed by a cryotherapy procedure, to reduce the incidence of cervical cancer. However, compliance with the program is still low in the targeted population. This study aims to see what factors influence women to receive cryotherapy treatment if they have positive VIA result. Methods: This cross-sectional study was conducted among 356 VIA positive women, aged 30-50 years old, registered at Temanggung District Health Office, Central Java, Indonesia between March 29 and April 31, 2018. Data on whether subjects underwent cryotherapy, their demographic profile, education, knowledge about cryotherapy, and family support were collected in a direct interview using a structured questionnaire. A statistical analysis was carried out to observe the influence of all the variables on subjects’ decisions on cryotherapy. Results: In our study, 217 women (60.69%) received cryotherapy, while 139 women (39.04%) did not. Among all the variables analyzed, the factors affecting the subjects’ likelihood to undergo cryotherapy are their knowledge about cervical cancer and screening (PR=0.776;95%CI=0.660-0.913;p=0.003), their residences distance from health centre (PR=0.795;95%CI=0.650-0.971;p=0.016), permission from their family (PR=0.675;95%CI=0.556-0.820;p=0.018), and being accompanied by their family (PR=0.824;95%CI=0.700-0.970;p=0.026). Age, marital status, occupation, and education background did not show a significant correlation with the women’s decisions to receive cryotherapy. Conclusions: Interestingly, the result of our study indicates that women are less willing to undergo the cryotherapy procedure if they have good knowledge about the cryotherapy procedure and its importance in cervical cancer’s prevention. Providing higher quality and more accessible health facilities with cryotherapy services are important in influencing women’s willingness to receive cryotherapy. Family support, in the form of permission given by spouses, and if they accompanied the patient to seek cryotherapy care are observed as factors influencing women’s willingness to have the procedure.<br /> https://journal.waocp.org/article_89067_2ed5794eff6a3d6d3891b54cc1d8c659.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Simultaneous Effects of Viral Factors of Human Papilloma Virus and Epstein-Barr Virus on Progression of Breast and Thyroid Cancers: Application of Structural Equation Modeling143114398905610.31557/APJCP.2020.21.5.1431ENShayanMostafaeiMedical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.AnoshirvanKazemnejadDepartment of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Amir HosseinNorooznezhadMedical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Regenerative Medicine
Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.0000-0002-9987-7093BehzadMahakiDepartment of Biostatistics, School of Health,
Kermanshah University of Medical Sciences, Kermanshah, Iran.0000-0001-7062-6971MohsenMoghoofeiMedical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.Department of Microbiology, Faculty of Medicine, Kermanshah
University of Medical Sciences, Kermanshah, Iran.Journal Article20200126This study aimed to assess effects of the sets of EBV and HPV expressed proteins simultaneously on the sets of cellular/inflammatory factors in breast and thyroid cancers using structural equation modeling. In this multi-center case-control study, according to the inclusion and exclusion criteria, 83 breast and 57 thyroid specimens were collected from the eligible patients. In addition, 31 and 18 histopathological evaluated normal breast and thyroid samples were also examined as age-matched healthy controls. In addition, ELISA and Real-time PCR were used to measure the expression level of viral and cellular/inflammatory genes and proteins. Structural equation modeling was used to test the causal associations between the sets of EBV and HPV expressed proteins with inflammatory factors in breast and thyroid cancers development. Breast cancer patients had a higher incidence of HPV-positively and EBV-positively than healthy controls (OR=1.66, 95%CI=0.79-3.47, P-value=0.177), (OR=3.18, 95%CI=1.52-6.63, P-value=0.002), respectively. In addition, thyroid cancer patients had a significantly higher incidence of EBV-positivity than healthy controls (OR=3.72, 95% CI=1.65-8.36, P-value=0.001). After fitting the SEM model, HPV proteins factor has significant direct and total effects on the cellular/inflammatory factors in breast cancer (direct effect: β=0.426, P-value=0.01; total effect: β=0.549, P-value<0.001). However, EBV proteins factor has most significant total effect on the cellular/inflammatory factors in breast cancer (total effect: β=0.804, P-value<0.001) than the cellular/inflammatory factors in thyroid cancer (total effect: β=0.789, P-value<0.001). For the first time, a significant association between EBV and HPV -genes, anoikis resistance and the development of breast and thyroid cancers demonstrated by using SEM, Simultaneously.<br /> https://journal.waocp.org/article_89056_0b037f3ae6ee1dc3572ad36e8438b96f.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Effectiveness of a Transanal Drainage Tube for the Prevention of Anastomotic Leakage after Laparoscopic Low Anterior Resection for Rectal Cancer144114448907510.31557/APJCP.2020.21.5.1441ENZhengWangDepartment of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, China.JianweiLiangDepartment of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, China.JiananChenDepartment of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, China.ShiwenMeiDepartment of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, China.QianLiuDepartment of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,
Chinese Academy of Medical Sciences and Peking Union Medical College, China.Journal Article20200201Background and Objective: Anastomotic leakage is one of the most serious complications after laparoscopic low anterior resection Low Anterior Resection (LAR) for rectal cancers. The purpose of this study was to evaluate the effectiveness of a transanal drainage tube placed for the prevention of anastomotic leakage after laparoscopic LAR. Methods: The clinical data of 220 patients with rectal cancer who underwent laparoscopic LAR using the double stapling technique Double Stapling Department of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Technique (DST) from Jun 2017 to Dec 2018 were analyzed retrospectively at our institution. A transanal drainage tube was placed after anastomosis in 120 patients (TDT group). Another 100 patients were operated on without a transanal drainage tube (NTDT group). Clinicopathological and surgical factors, the frequencies of anastomotic leakage and re-operation after leakage were compared between the two groups. Results: Patient age, gender, body mass index, American Society of Anesthesiologists (ASA) score, previous abdominal surgery, intraoperative blood loss, tumor size, tumor stage, specimen length, distance of tumor from the anal verge, and operative time were comparable between the two groups. Overall rate of leakage was 4.5% (10/220). The frequency of leakage was 3.3% (4/120) in TDT group and was 6.0% (6/100) in NTDT group. The rate of leakage was significantly lower in TDT group (p<0.05). Furthermore, the re-operation rate for symptomatic anastomotic leakage was 50.0% (2/4) in TDT group, while in contrast it was 83.3% (5/6) in NTDT group. The rate of re-operation was lower in TDT group than NTDT group (p<0.05). Conclusions: The use of a transanal drainage tube in laparoscopic LAR for rectal cancer is a simple and effective method for prevention of anastomotic leakage and decreases the rate of re-operation after symptomatic leakage.https://journal.waocp.org/article_89075_11f09aa63c57f9e6d7efd2fc1a0d6f66.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Genetic Variations of RAD51 and XRCC2 Genes Increase the Risk of Colorectal Cancer in Bangladeshi Population144514518906110.31557/APJCP.2020.21.5.1445ENAnika UddinHridyDepartment of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka-1205, Bangladesh.SamiaShabnazDepartment of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka-1205, Bangladesh.M DAsaduzzamanDepartment of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka-1205, Bangladesh.MohammadShahriarDepartment of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka-1205, Bangladesh.Mohiuddin AhmedBhuiyanDepartment of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka-1205, Bangladesh.Mohammad SafiqulIslamDepartment of Pharmacy,
Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.0000-0003-4924-5319S M MoazzemHossenDepartment of Pharmacy, Faculty of
Biological Science, University of Chittagong, Chittagong-4331, Bangladesh.Talha BinEmranDepartment of Pharmacy, BGC Trust University
Bangladesh, Chandanaish, Chittagong-4381, Bangladesh.0000-0003-3188-2272Journal Article20200205Objectives: In case of Bangladeshi population, no report is observed till now showing the genetic variations of RAD51 (rs1801320) and XRCC2 (rs3218536) genes polymorphism having association with colorectal cancer risk. For this reason the aim of this study is to ascertain their interrelation with colorectal cancer occurrence in Bangladeshi population. Materials and Methods: A case control study was conducted where 200 colorectal cancer patients and 200 healthy volunteers were figured for this research using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Results: Here, in case of RAD51 (rs1801320), G/C heterozygous genotype was found significant (p=0.037; OR=1.64; 95% CI=1.03 to 2.6). On the other hand, G/G genotype was not found statistically significant (p=0.423; OR=1.61; 95% CI=0.49 to 5.22) and significance was observed for GC+GG (p=0.030; OR=1.63; 95% CI=1.05 to 2.55). In case of XRCC2 (rs3218536), C/T heterozygous genotype was remarked statistically significant (p=0.033; OR=1.60; 95% CI=1.04 to 2.46). The T/T genotype was not recorded statistically significant (p=0.237; OR=1.65; 95% CI=0.72 to 3.76) but significance found for CT+TT (p=0.027; OR=1.61; 95% CI=1.05 to 2.45). Moreover, it is found that the risk factor of developing CRC is observed in G/C, C/T heterozygote and GC+GG, CT+TT (heterozygote+ mutant) in RAD51 (rs1801320) and XRCC2 (rs3218536) respectively although no significance is observed in case of G/G and T/T mutant. Conclusions: So, the association of RAD51 (rs1801320) and XRCC2 (rs3218536) genes polymorphism with colorectal cancer risk is observed in Bangladeshi population.https://journal.waocp.org/article_89061_a413a337c9ad386657743fc7638d9726.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501LAT1 (SLC7A5) Overexpression in Negative Her2 Group of Breast Cancer: A Potential Therapy Target145314588907610.31557/APJCP.2020.21.5.1453ENKhaldonBodoorDepartment of Applied Biology, Jordan University of Science and Technology, Irbid, Jordan.RowidaAlmomaniDepartment of Medical Laboratory
Sciences, Jordan University of Science and Technology, Irbid, Jordan.MohammadAlqudahDepartment of Pathology, Jordan University of Science
and Technology, Irbid, Jordan.0000-0001-6877-1184YazanHaddadDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno,
Czech Republic.Central European Institute of Technology, Brno University of Technology, Purkynova, Brno, Czech Republic.WalaaSamouriDepartment of Pathology, Jordan University of Science
and Technology, Irbid, Jordan.Journal Article20200209Objective: HER2 negative carcinomas of the breast pose a challenge for treatment due to redundancies in potential drug targets and poor patient outcomes. Our aim was to investigate the role of L-type amino acid transporter – LAT1 as a potential prognosticator and a drug target. Methods: In this retrospective work, we have studied the expression of LAT1 in 145 breast cancer tissues via immunohistochemistry. Overall survival analysis was used to evaluate patient outcome in various groups of our cohort. Results: Positive LAT1 expression was found in 27 (84.4%) luminal A subtype, 27 (64.3%) luminal B/triple positive subtype, 29 (82.9%) triple negative subtype, and 24 (66.7%) HER2-only positive subtype (p=0.1). Interestingly, negative correlation was found between LAT1 and HER2; where positive expression of LAT1 was found in 56 (83.6%) cases in negative HER2 group and 51 (65.4%) cases from positive HER2 group (p=0.01). Unfortunately, we were unable to report significant survival differences when LAT1 expression was studied in the negative HER2 group. Nevertheless, five incidents of mortality (out of 55) were reported in LAT1+/HER2- group compared to none in the LAT1-/HER2- group (N=11). Conclusion: Our findings of overexpression of LAT1 in negative HER2 group suggest a role of this protein as prognosticator and drug target in a challenging therapeutic cohort.<br /> https://journal.waocp.org/article_89076_d5e247de2d6e62848f75bd880bca6967.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Co-existing of HSV1/2 or EBV Infection with the Presence of High-Risk HPV DNA in Cervical Lesions in the Southwest of Iran145914648907310.31557/APJCP.2020.21.5.1459ENNegarJohariniaDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.SajadFaghihinejadDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.KeyvanSeyediDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.AliFarhadiDiagnostic
Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz,
Iran.Seyed YounesHosseiniDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.AkbarSafaeiDepartment of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.HelenBahrampourDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.JamalSarvariDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Gastroenterohepatology
Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Journal Article20200212Objective: While the vast majority of the cervical lesions have been attributed to the HPVs, the role of EBV and HSV1/2 as co-factors in the progression of these abnormalities needs more investigation. In this study, we aimed to determine the co-existence of EBV or HSV in cervical lesions infected with high-risk HPVs. Methods: Totally, 102 formaline-fixed cervical lesions with different pathological grades (LSIL, HSIL, and SCC) were enrolled in this study. DNA was extracted, and its integrity was examined by PCR assay. Two conventional PCRs were performed for the detection of EBV and HSV1/2 genomes in the tissue specimens. Besides, an in-house Real-Time PCR, as well as a nested PCR assays following sequencing, was performed to detect HPV genotypes in EBV or HSV positive samples. Results: The mean age of the participants was 42.8±13 years. Out of 102 samples, 32% (n=33) were confirmed to be LSIL, 42.2% (n=43) were HSIL, 22.5% (n=23) were SCC and 2.9% (n=3) were adenocarcinoma. EBV genome was detected in 13(12.7%) samples including 2 of LSIL, 8 of HSIL and 3 of SCC. All EBV positive samples harbored high risk HPV types 16,18 and/or 31 co-infections. However, the HSV genome was not found in any of the samples. Conclusion: Our result revealed that the frequency of EBV infection is higher in HISL than LSIL. Moreover, the amount of HPV load showed an elevated level among co-infected patients, which indicates that EBV might be an enhancing factor of disease progression. In contrast, HSV may not has a role as a co-factor in cervical lesions pathogenesis.https://journal.waocp.org/article_89073_58650a1ea997ffda7e3fbd2e53dff234.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Expression of HER-2/neu in Oral Squamous Cell Carcinoma146514708908210.31557/APJCP.2020.21.5.1465ENSanaMirzaDepartment of Oral Pathology, College of Dentistry, Ziauddin Medical University, Karachi, Pakistan.NailaHadiResearch and Development,
Islamabad Medical and Dental College, Shaheed Zulfiqar Ali Bhutto Medical University, Ilamabad, Pakistan.ShahidPervaizDepartment of
Histopathology and Microbiology, Aga Khan University Hospital, Karachi, Pakistan.SultanZeb KhanDepartment of Clinical Pathophysiology,
Graduate School of Tokyo Dental College, 1-2-2 Masago, Mihama-Ku, Chiba 261-8502, Japan.Sameer AMokeemDepartment of Periodontics and
Community Dentistry, King Saud University, Riyadh, Saudi Arabia.TariqAbduljabbarDepartment of Prosthetic Dental Science, College of Dentistry,
King Saud University, Research Chair for Biological Research in Dental Health, Riyadh, Saudi Arabia.NawwafAl-HamoudiDepartment of Periodontics and
Community Dentistry, King Saud University, Riyadh, Saudi Arabia.FahimVohraDepartment of Prosthetic Dental Science, College of Dentistry,
King Saud University, Research Chair for Biological Research in Dental Health, Riyadh, Saudi Arabia.0000-0002-6204-7985Journal Article20200217Background: HER-2/neu is a member of the human epidermal growth factor (HER) family of transmembrane tyrosine kinases, which is significantly associated with the pathogenesis of various cancer types. The aim was to evaluate the expression of HER-2/neu in oral squamous cell carcinoma (OSCC) as a potential biomarker to target antigens for specific immunotherapy in OSCC. Methods: One hundred and forty histologically diagnosed OSCC cases were identified. Four to five-micrometer thick formalin-fixed, paraffin-embedded tumor sections were stained with Haematoxylin and Eosin (H and E). Histological grade was assessed according to WHO/Broders classification, while tumors were staged according to the American Joint Committee on Cancer (AJCC) TNM classification from stage I to IV. Immunohistochemistry was performed by using Rabbit monoclonal antibody against HER-2/neu (EP700Y, cell marquee and diluted 1:50). FISH was performed on positive cases using Vysis PathVysion HER-2 DNA probe (Abbott USA). Probes consist of LSI HER gene spectrum orange and control probe CEP 17 spectrum green. Results: In this study, males were mostly effected (64.3%) with buccal mucosa (49%) to be the commonly involved site for OSCC. Majority of cases were moderately differentiated (62.1%) and 50.7% tumors were Stage IV. HER-2/neu was found to be positive (2+) in one case of OSCC, however weak to moderate complete membrane staining was observed in >10% of the tumor cells. One hundred and thirty nine cases were HER-2/neu negative. FISH analysis of HER-2/neu positive cases also showed gene amplification (Her2-neu/ CEp 17 = 225/33 = 7.2). Conclusions: The study showed disparity in the expression of HER-2/neu in OSCC, which is due to multiple reasons. Therefore therapy against HER-2/neu in OSCC is debatable.https://journal.waocp.org/article_89082_4b3936f194401d725c9266689ca4a8a9.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Microsatellite Instability and Life Style Factors in Sporadic Colorectal Cancer147114808907810.31557/APJCP.2020.21.5.1471ENNoraAtefBiostatistics and Cancer Epidemiology department, National Cancer Institute (NCI), Cairo University, Egypt.NellyAlieldinBiostatistics and Cancer Epidemiology department, National Cancer Institute (NCI), Cairo University, Egypt.GhadaSherifBiostatistics and Cancer Epidemiology department, National Cancer Institute (NCI), Cairo University, Egypt.ImanLoayPathology
Department, NCI, Cairo University, Egypt.AhmedMostafa MahmoudSurgical Oncology department , NCI, Cairo University, Egypt.GhadaMohamedPathology
Department, NCI, Cairo University, Egypt.Journal Article20200226Background: Colorectal cancer (CRC) in Egypt is a relatively high young onset disease. As a form of heterogeneous cancer, there is interplay between genetic and environmental factors. We aimed at probing the association of life style factors and Microsatellite Instability (MSI) status that could provide more insights on carcinogenic process of CRC. Methods: One hundred incident sporadic CRC patients were involved. Information on risk factors of CRC was obtained and microsatellite instability status was predicted through evaluation of MMR protein expression via immunohistochemistry (IHC). Results: Median age was 47.50 years, females represented 54.0% and 36% of patients were Microsatellite Instability High (MSI-H). Most patients with right sided colon cancer (78.3%) were MSI-H while mostly stable or low MSS/MSI-L for left-sided colon and rectum (78.6%, 74.3% respectively, p<0.001). Patients with low physical activity had higher risk of MSS/MSI-L than those with moderate or high activity p =0.026. Patients with BMI greater than 30 Kg/m2 had higher MSS/MSI-L (75.5%) than those with BMI between 25-30 Kg/m2 (60.6%) and those with normal BMI https://journal.waocp.org/article_89078_4c37ee67974ce2c779a97cfdfec88d68.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Patterns of Care and Outcome Analysis of Nasopharyngeal Carcinoma: An Indonesian Single Institution Study148114858909210.31557/APJCP.2020.21.5.1481ENHamida HayatiFaisalDepartment of Ear, Nose and Throat, Head and Neck Surgery, Dr. Cipto Mangunkusumo National General Hospital, Faculty
of Medicine Universitas Indonesia, Jakarta, Indonesia.NobuteruKuboDepartment of Radiation Oncology, Gunma University Graduate
School of Medicine, Gunma, Japan.EndangNuryadiDepartment of Radiation Oncology, Gunma University Graduate
School of Medicine, Gunma, Japan.Department of Radiation Oncology, Dr. Cipto Mangunkusumo National General
Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.0000-0003-1029-1748JoedoPrihartonoDepartment of Community Medicine, Faculty of Medicine
Universitas Indonesia, Jakarta, Indonesia.Tubagus DjumhanaAtmakusumaDepartment of Internal Medicine, Hematology and Medical Oncology Division, Dr.
Cipto Mangunkusumo National General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.LisnawatiRachmadiDepartment of
Pathological Anatomy, Dr. Cipto Mangunkusumo National General Hospital, Faculty of Medicine Universitas Indonesia, Jakarta,
Indonesia.000-0002-5307-3671TakahiroOikeDepartment of Radiation Oncology, Gunma University Graduate
School of Medicine, Gunma, Japan.TakashiNakanoDepartment of Radiation Oncology, Gunma University Graduate
School of Medicine, Gunma, Japan.Soehartati AGondhowiardjoDepartment of Radiation Oncology, Dr. Cipto Mangunkusumo National General
Hospital, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.0000-0002-9446-4361MarlindaAdhamDepartment of Ear, Nose and Throat, Head and Neck Surgery, Dr. Cipto Mangunkusumo National General Hospital, Faculty
of Medicine Universitas Indonesia, Jakarta, Indonesia.Journal Article20191225Background: Nasopharyngeal cancer is endemic to Southeast Asia. However, there is limited clinical evidence of nasopharyngeal cancer in Indonesia, which has the largest population in Southeast Asia. Methods: Patterns of care and treatment outcomes in 428 patients with newly-diagnosed and pathologically-confirmed nasopharyngeal cancer were retrospectively analyzed. Results: Concurrent chemo-radiotherapy (CCRT) was the first-line treatment for stages I–IVB diseases. The 2-year overall survival (OS) of all patients were 100.0%, 100.0%, 93.8%, 86.2%, 82.9%, and 62.4% for stages I, II, III, IVA, IVB, and IVC, respectively. The 2-year OS of CCRT-treated patients were 100.0%, 100.0%, 92.6%, 82.4%, and 78.3% for stages I, II, III, IVA, and IVB, respectively. Conclusion: The patterns of care and treatment outcomes were potentially consistent with world standards, needing future validation. This is the largest study of newly diagnosed nasopharyngeal cancer in Indonesia, a huge disease burden, providing an important basis for the clinical management of this disease.https://journal.waocp.org/article_89092_7ab4eac0ff903fc12c7c06c845b8a82e.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501GLOBAL Leukemia in Children 0-14 Statistics 2018, Incidence and Mortality and Human Development Index (HDI): GLOBOCAN Sources and Methods148714948909410.31557/APJCP.2020.21.5.1487ENSeyedeh MahdiehNamayandehPrevention and Epidemiology Research Center of Non-Communicable Disease, Health Faculty, Shahid
Saduoghi University of Medical Sciences, Yazd, Iran.ZaherKhazaeiDepartment of Public Health, School of Medicine, Dezful University of
Medical Sciences, Dezful, Iran.0000-0001-8619-3664MoslemLari NajafiPharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical
Sciences, Kerman, Iran.0000-0002-4170-2792ElhamGoodarziSocial Determinants of Health Research Center, Lorestan University of Medical Sciences, Khorramabad,
Iran.AlirezaMoslemIranian Research Center on Healthy Aging, Sabzevar University of Medical Sciences, Sabzevar, Iran.Journal Article20200113Objective: Cancer is the second leading cause of death in children under 15 and leukemia is the most common type of cancer in this age group. The aim of the present study is to investigate the incidence and mortality of leukemia in children aged 0-14 years and its relationship with Human Development Index (HDI in different countries of the world. Methods: Incidence and mortality rates were obtained from GLOBOCAN and Country’s income from World Bank. The data analysis was conducted using correlation analysis. The association of incidence and mortality rates with HDI was investigated using linear regression models. Results: The results revealed a significant positive correlation between the incidence rate and Gross National Income per capita (r = 0.464, P <0.0001), mean years of schooling (r = 0.566, P <0.0001), life expectancy at birth (r = 0.712, P <0.0001) and expected years of schooling (r = 0.604, P <0.0001). The results also demonstrated a positive and significant correlation between mortality rate and life expectancy at birth (r = 0.199, P <0.0001). An improvement in HDI [Beta = 7.7, CI95% (0.1, 15.3)] and life Expectancy at birth [Beta = 0.1, CI95% (0.03, 0.1)] caused a significantly rise in the incidence of leukemia. Moreover, the improved HDI [Beta = 6.2, CI95% (1.9, 10.5)] was associated with increased mean years of schooling [Beta = -0.1, CI95% (-0.2, -0.01)] and expected years of schooling [Beta = -0.1, CI95% (-0.3, -0.08). Conclusion: As the HDI increases, incidence and mortality from of leukemia increases indicating a change in factors that affects leukemia incidences.https://journal.waocp.org/article_89094_439fb53eadaa109a8e988b41a558e4f7.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736821520200501Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells. Asian Pac J Cancer Prev. 2018; 19(12): 3501-3509. doi: 10.31557/APJCP.2018.19.12.3501. Roopesh Krishnankutty et al.149514958909310.31557/APJCP.2020.21.5.1495ENJournal Article20200525https://journal.waocp.org/article_89093_c289fe145295adaca4b00e3b56be33b9.pdf