West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201The Metastatic Spread of Breast Cancer Accelerates during Sleep: How the Study Design can Affect the Results3533559051210.31557/APJCP.2023.24.2.353ENMohammadHoushyariDepartment of Clinical Oncology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.FarzadTaghizadeh-HesaryENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Radiation Oncology, Iran University of Medical Sciences, Tehran, Iran.0000-0002-6195-2203Journal Article20230204Metastasis is the most common event that determines survival in patients with breast cancer. The benefits of appropriate sleep in enhancing cancer patients' prognosis have been demonstrated. Likewise, emerging evidence has noted the positive impacts of regular circadian rhythm on cancer survival. Proper sleep and regular circadian rhythm can help to improve the cancer prognosis by enhancing the immune system. Besides, circadian rhythm disruption can assist cancer progression by promoting systemic inflammation. However, a recent study by Diamantopoulou et al. titled “The Metastatic Spread of Breast Cancer Accelerates during Sleep” demonstrated that sleep can aggravate breast cancer metastasis. This article outlines how the study design can affect this controversy.https://journal.waocp.org/article_90512_7b7352bc67336a1dc562f3e6de65eb69.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201DNA Damage on Buccal Epithelial Cells, Personal Working in the Rubber Industry Occupationally Exposed to Carbon Disulfide (CS2)3573619050310.31557/APJCP.2023.24.2.357ENManikantanPappuswamyDepartment of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India.AditiChaudharyDepartment of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India.ArunMeyyazhaganDepartment of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India.Karthick KumarAlagamuthuDepartment of Biotechnology, Selvam Arts and Science College, Namakkal, Tamilnadu, India.BalamuralikrishnanBalasubramanianDepartment of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul 05006, Republic of Korea.Vijaya AnandArumugamMedical Genetics and Epigenetics Laboratory, Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.Joseph KadanthottuSebastianDepartment of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India.Journal Article20221001Introduction: The most significant industrial utilization of carbon disulfide (CS2) has been in the manufacture of cellulose rayon, cellophane, and rubber industry. CS2 prompts expanded recurrence of chromosomal variations in laborers occupationally exposed to CS2. Materials and Methods: In the current study, the DNA analysis was carried out from exfoliated buccal epithelial cells from rubber industry workers exposed to CS2 and an equal number of healthy control subjects. Both the control and experimental subjects were categorized by their smoking habits such as smokers (S) and non-smokers (NS). Furthermore, experimental subjects were further separated based on their exposure period. Students t-test statistical tools were used to analyze the final results. Results: The present analysis identified a high frequency of DNA damage in rubber industry workers (16.55±0.43) than control subjects (9.8±0.21). Also, maximum number of DNA damage detected in smoking experimental group (18.27±0.02) than non-smoking experimental (15.02±0.01) and smoking control groups (10.25±0.04 ). Conclusion: Smoking habits synergistically increased the DNA damage in the rubber industry workers exposed to CS2.https://journal.waocp.org/article_90503_b9e9efc1a22577da140b9b3b44c89db4.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Exosome Mediated Cancer Therapeutic Approach:Present Status and Future Prospectives3633739048110.31557/APJCP.2023.24.2.363ENAnithaRoyDepartment of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Velappanchavadi, PH Road, Chennai, Tamil Nadu, India.Department of Pharmacology, Saveetha College of Allied Health Sciences, Saveetha Institute of Medical and Technical Sciences [SIMATS], Thandalam, Tamil Nadu, India.SmilineGirija ASDepartment of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Velappanchavadi, PH Road, Chennai, Tamil Nadu, India.PitchaipillaiSankar GaneshDepartment of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Velappanchavadi, PH Road, Chennai, Tamil Nadu, India.MuthupandianSaravananDepartment of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences [SIMATS], Saveetha University, Velappanchavadi, PH Road, Chennai, Tamil Nadu, India.BasilSunnyClinical Pharmacologist, Department of Pharmacy, Sunrise Hospital, Kochi, Kerala, India.Journal Article20220524The unique extracellular vesicles (EVs) or exosomes formed by the sequential invagination of the plasma membrane are diverse and encompass important constituents with biological functions. Speculations on its cell independent biological functions are significant and pose them as vital biomarkers and as drug delivery vehicles especially in cancer. EVs possess theragnostic values and are known to elicit specific immune response. Exosomes can also serve as potential nanocarriers for delivering miRNA, siRNA, anti-cancer drugs and membrane-associated proteins. Exosomes play a crucial role in regulating tumour progression, metastasis, and angiogenesis. This review thus portrays the multiple facets of exosomes, in concert with the source for exosomes production and further on its regulation and intercellular communication. The review also explores the recent advances, present status and the future prospective in the application of exosomes in cancer therapeutics and cancer diagnostics.https://journal.waocp.org/article_90481_d3bd41777d534f8f2755040aedfa93a0.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Unfolding of Imminent Bio-Signatures in the Prognosis of Thyroid Cancer; The Emergence of Estrogen Related Receptor Gamma (ERRγ) as a Hurricane3753879048910.31557/APJCP.2023.24.2.375ENDeepakGulwaniDepartment of Medical Oncology Laboratory, All India Institute of Medical Sciences, New Delhi, India.PriyankaUpadhyayDepartment of Medical Oncology Laboratory, All India Institute of Medical Sciences, New Delhi, India.RidhimaGoelDepartment of Medical Oncology Laboratory, All India Institute of Medical Sciences, New Delhi, India.VijayaSarangthemDepartment of Pathology,
All India Institute of Medical Sciences, New Delhi, India.ThoudamDebraj SinghDepartment of Medical Oncology Laboratory, All India Institute of Medical Sciences, New Delhi, India.0000-0003-0750-9136Journal Article20220809Thyroid cancer’s incidence has increased by leaps and bounds over the last years and accounts for 2.8% of new cases of cancers. This increasing bar is partially assisted by enormous screening to understand the sub-clinical status. Advanced tumor growth is the leading cause of thyroid cancer-associated death. However, the complete understanding of the underlying cause is still to be disclosed. The updated clinical assessment evidenced a few major oncogenes viz. RAS, BRAF, and RET as key drivers in the development and progression of thyroid cancer. The BRAF mutation, a major cause of aggressive tumor type in papillary thyroid carcinoma, is frequently reported. The characteristic oncogenic changes imply thyroid cancer to be clinically an ideal model for targeted therapy against RET, RAS, and BRAF mutation. Though the sensitive biochemical marker assay has been improvised, the diagnosis of thyroid follicular neoplasms is still a big challenge as the biopsy aspiration cannot define the nature of the tumor in 30% of the cases. The main hurdle is assisted distinction between follicular thyroid lesions. The discrimination between follicular thyroid adenomas and carcinomas is histologically accomplished. This strictly necessitates the identification of sensitive diagnostic/prognostic markers to mitigate the risk of thyroid cancer and to avoid the unnecessary hurdles of biopsy and surgery. An array of prognostic biomarkers is being used for the diagnosis of thyroid cancer. However, Estrogen Related Receptor Gamma (ERRγ) is setting a new benchmark among the clinical biomarkers. The dramatic expression of ERRγ in thyroid cancer enables itself not only to serve as a characteristic diagnostic marker but also as a therapeutic target. Recently, we have reported that ERRγ is upregulated in 96 papillary thyroid cancer (PTC) and 26 poorly differentiated/ anaplastic thyroid cancer (ATC) samples. Various synthetic ERRγ inverse agonists viz. GSK5182, DN200434, and 24e are fully proved to modulate ERRγ expression in ATC to attain partial cure. If this finding can be assayed on a larger scale the evaluation of this marker may be warranted and informative. This review article highlights the ascending sheds of clinical biomarkers of thyroid cancer. This also reveals the clinical importance of ERRγ as an evolving diagnostic and therapeutic target in thyroid cancer.https://journal.waocp.org/article_90489_0663d658113ade0597df9d24ad91ef3e.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Efficacy and Safety of Topical Tacrolimus in Comparison with Topical Corticosteroids, Calcineurin Inhibitors, Retinoids and Placebo in Oral Lichen Planus: An Updated Systematic Review and Meta-Analysis3894009049410.31557/APJCP.2023.24.2.389ENJanicePintoDepartment of Oral Medicine and Radiology, D Y Patil University, School of Dentistry, Navi Mumbai, India.MandaviWaghmareDepartment of Oral Medicine and Radiology, D Y Patil University, School of Dentistry, Navi Mumbai, India.0000-0001-8919-1218KetakiBhorDepartment of Public
Health Dentistry, D Y Patil University, School of Dentistry, Navi Mumbai, India.0000-0003-3847-4319VasaviSantoshDepartment of Oral Medicine and Radiology, D Y Patil University, School of Dentistry, Navi Mumbai, India.ReemaManojDepartment of Oral Medicine and Radiology, D Y Patil University, School of Dentistry, Navi Mumbai, India.SejalSamsonDepartment of Oral Medicine and Radiology, D Y Patil University, School of Dentistry, Navi Mumbai, India.Journal Article20220915Background: Tacrolimus is a powerful macrolide calcineurin inhibitor that has low adverse effects which lead to a rapid response in the control of signs and symptoms in comparison to that of corticosteroids in Oral Lichen Planus(OLP). There have been increasing number of studies establishing the use of topical tacrolimus in oral lichen planus. Still, there is a need to find evidence of the successful use of tacrolimus in comparison to other drugs used in the treatment of OLP, by means of a systematic review and meta-analysis, so that an informed and accurate approach can be utilized. Methods: A comprehensive literature review was performed, including PubMed, the Cochrane Library, published up to and including December 2021. There were no restrictions on date of publication. Articles available in English language were included. Using the Cochrane Collaboration tool, we assessed the risk of bias for randomized controlled trials. A meta-analysis was performed on the relevant studies. Results: A total of 11 RCTs evaluating the effects of tacrolimus were included in this study after application of inclusion and exclusion criteria. Seven studies revealed a low bias risk, three presented a moderate risk and one had a high risk of bias. The results revealed no significant difference in clinical resolution and adverse effects between tacrolimus and corticosteroids. The pooled data from our meta-analysis shows that there is not sufficient evidence to prove that Tacrolimus is better in efficacy than other topical corticosteroids. Conclusion: According to the current systematic study and meta-analysis, there is not sufficient evidence to prove that Tacrolimus is better in efficacy than other drugs. Uniform trials are required with larger sample sizes and standardized methodology are required for a better analysis.https://journal.waocp.org/article_90494_d869f86cd11116f35983d4ea89e8c650.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Value of Conventional MRI, DCE-MRI, and DWI-MRI in the Discrimination of Metastatic from Non-Metastatic Lymph Nodes in Rectal Cancer: A Systematic Review and Meta-Analysis Study4014109049910.31557/APJCP.2023.24.2.401ENArvinArianMD Radiology, TUMS (Cancer Institute‑ADIR), Tehran University of Medical Sciences (TUMS), Tehran, Iran.Hayder JasimTaherTechnology of Radiology and Radiotherapy Department, Tehran University of Medical Sciences, International Campus, Tehran, Iran.HayderSuhail Najm AlareerRadiology Department, Collage of Health & Medical Technology, Al-Ayen University, Thi-Qar, Iraq.MehdiAghiliDepartment of Radiation Oncology, Cancer Institute, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.Journal Article20220924Background: Today, despite many studies on the diagnosis of metastasis to lymph nodes (LNs) in Rectal Cancer (RC), its diagnosis is still very challenging for radiologists. The purpose of the present study was to the assessment of the diagnostic value of conventional MRI, DCE-MRI, and DWI-MRI in the discrimination of metastatic from non-metastatic lymph nodes in RC. Methods: In the present meta-analysis study, we surveyed international databases including PubMed, Scopus, Embase, and Science Direct with appropriate keywords. Using the binomial distribution formula, the variance of each study was calculated and the data were analyzed using STATA version 14. Finally, the results of the studies were entered into the random-effects meta-analysis. Also, we used the chi-squared test and I2 index to calculate heterogeneity among studies, and for evaluating publication bias, Funnel plots and Egger tests were used. Results: 31 articles published between 2005 and 2021, comprising 2517 patients were included in the present study. The sensitivity and specificity of DCE-MRI were 83% (74% to 80%), and 86% (80% to 93%), respectively with PPV 84% (76% to 89%) and NPV 88% (79% to 95%). Also, the sensitivity and specificity of DWI-MRI were 81% (74% to 88%), and 74% (78% to 91%), respectively with PPV 63% (54% to 74%), NPV 85% (77% to 93%), AUC 80 % (75% to 86%) and accuracy 82% (75% to 88%). For conventional MRI, the sensitivity 74% (67% to 80%), specificity 77% (71% to 83%), PPV 62% (48% to 69%), NPV 70% (62% to 77%), AUC 78% (72% to 83%) and 71% accuracy (68% to 78%) was obtained. Conclusion: Based on our finding DCE-MRI is the most suitable technique for the discrimination of metastatic lymph nodes in rectal cancer.https://journal.waocp.org/article_90499_1f1e7f7a505dd5ab827270c0680cfc02.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Polymorphism Analysis of Interleukin-18 and Interleukin-37 Genes in Hepatitis B Infections with Different Outcomes: A Preliminary Report from an Iranian Population4114169051610.31557/APJCP.2023.24.2.411ENVahidMolaeiDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Mohammad RezaFattahiGastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Mohammad RezaHaghshenasShiraz Institute for Cancer
Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Seyed YounesHosseiniDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Seyed AliMalekhosseiniTransplant Research Center, Shiraz University
of Medical Sciences, Shiraz, Iran.JamalSarvariDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Journal Article20210228Objective: Given the vital role of cytokines in influencing the outcomes of hepatitis B virus (HBV) infections, this study aimed to investigate the association between polymorphisms of interleukin (IL)-18 and IL-37 and the outcomes of HBV infection. Methods: In this study, we enrolled 300 subjects with chronic HBV infection, including those with cirrhosis/hepatocellular carcinoma (C/HCC), chronic active hepatitis B (CAH) infection, or asymptomatic carriers (AC), and 58 individuals whose infection was spontaneously cleared (SC). Genomic DNA was extracted, and IL-18/IL-37 genotyping was performed using PCR-RFLP and ARMS-PCR. Results: The frequency of genotypes and alleles of IL-18 single nucleotide polymorphisms (SNPs) at positions rs1946519, rs1946518, and rs187238 and IL-37 at position rs4241122 were not statistically different among the four studied groups (P>0.05). Furthermore, the frequency of different haplotypes was similar among the studied groups (P>0.05). Conclusions: Polymorphisms of IL-18 SNPs at positions rs1946519, rs1946518, and rs187238 and variation of IL-37 at position rs4241122 do not appear to influence the outcome of HBV infection.https://journal.waocp.org/article_90516_c56294263d2a1d6c3df2bf73854458b8.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Prognostic Factors for the Long-Term Survival after Hematopoietic Stem Cell Transplantation in Patients with Hodgkin Lymphoma4174239047710.31557/APJCP.2023.24.2.417ENDariushKadkhodaDepartment of Biostatistics, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0001-5728-0259MaryamNikoonezhadDepartment of Immunology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran.Ahmad RezaBaghestaniDepartment of Biostatistics, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0003-2813-6762SayehParkhidehHematopoietic Stem Cell
Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran University of Medical Sciences, Tehran, Iran.0000-0002-5200-8915ZahraMomeni-VarposhtiHematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Ali AkbarKhadem MaboudiDepartment of Biostatistics, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0001-8980-0108Journal Article20220418Objectives: This study investigated the possible prognostic factors for the long-term survival (Cure Rate) of Hodgkin Lymphoma patients who underwent HSCT. Methods: This retrospective cohort study analyzed 116 Patients diagnosed with Hodgkin Lymphoma who received autologous hematopoietic stem cell transplantation (Auto-HSCT) between the years 2007 and 2014 and followed up until 2017. The information regarding patients’ survival had been collected using phone calls, and their pre-transplant information was available in the archived documents. Prognostic effects were investigated using long-term survival models. Results: Patients with obesity had five times higher odds of long-term survival (cure) than the others (P=0.06). Also, the recurrence experience after HSCT negatively impacted the curing potential by 78% (P=0.05). Also, with 32 years as the change point, patients younger than 32 had 76% fewer odds of surviving long-term (P=0.03), and Poor transfused stem cell dose of CD34+ (<0.16 × 106 cells/ml) reduced the odds of long-term survival by 92% (P=0.01). Conclusion: According to the statistical models used in this study, obesity can increase the curing potential of Hodgkin lymphoma after transplantation. Meanwhile, aging, poor transfused CD34+ cells, and recurrence after HSCT were associated with lower survival following HSCT.https://journal.waocp.org/article_90477_e1e78482ee714e89eb83b0d9301e9a71.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Potentiation of TRAIL-Induced Apoptosis in TRAIL-Resistant Cholangiocarcinoma Cells by Curcumin through the Induction of DR5 Membrane Localization and Disruption of the Anti-Apoptotic Complex DR5/DDX3/GSK3β4254349051310.31557/APJCP.2023.24.2.425ENDaraneeVisitnonthachaiLaboratory of Pharmacology, Chulabhorn Research Institute, Thailand.WatanyooNakareangritTranslational Research Unit, Chulabhorn Research
Institute, Bangkok, Thailand.SumitraSuntararuksLaboratory of Pharmacology, Chulabhorn Research Institute, Thailand.KarnjanaChaiyotLaboratory of Pharmacology, Chulabhorn Research Institute, Thailand.PiyajitWatcharasitLaboratory of Pharmacology, Chulabhorn Research Institute, Thailand.Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Thailand.JutamaadSatayavivadLaboratory of Pharmacology, Chulabhorn Research Institute, Thailand.Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Thailand.Journal Article20220420Objective: Cholangiocarcinoma (CCA) is a cancer of the bile duct with a poor prognosis. The present study examined the ability of curcumin to sensitize apoptosis in the TNF-related apoptosis-inducing ligand (TRAIL)-resistant CCA cell lines of HuCCA-1 and KKU-213A. Methods: Apoptosis was measured using a TUNEL assay. Protein expression was determined by immunoblotting. Membrane death receptor 5 (DR5) was detected by flow cytometry. Protein complex was examined by co-immunoprecipitation. Result: Curcumin potentiated TRAIL-induced apoptosis in both cell lines, indicating the sensitization to TRAIL-induced apoptosis by curcumin. Additionally, curcumin increased DR5 expression and membrane localization; however, the curcumin/TRAIL combination did not result in further increases in DR5 expression and membrane localization in either cell line. Moreover, the curcumin/TRAIL combination reduced DR5/decoy receptor 2 (DcR2) complexes in both cell lines, suggesting that curcumin may enhance TRAIL-induced apoptosis by disrupting DR5/DcR2 interaction. In addition, levels of the anti-apoptotic complex DR5/ DDX3/GSK3β were reduced by the curcumin/TRAIL combination in HuCCA-1 but not in KKU-213A cells. This study also demonstrated that the DR5/DcR2 and DR5/DDX3/GSK3β complexes could be observed under basal conditions, suggesting that these anti-apoptotic complexes may contribute to TRAIL-resistant phenotypes in both cell lines. Pretreatment with the antioxidant N-acetylcysteine attenuated curcumin-enhanced apoptosis by TRAIL, indicating that curcumin sensitized TRAIL-induced apoptosis through an oxidative stress–dependent mechanism. Conclusion: The present study demonstrates the potential of using curcumin in combination with TRAIL to yield better TRAIL therapy outcomes in TRAIL-resistant CCA.https://journal.waocp.org/article_90513_3f77856813ce10c7dcb946d42d08649e.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Prevalence and Distribution of High- and Low- Risk HPV Genotypes in Women Living in the Metropolitan Area of Naples: A Recent Update4354419051710.31557/APJCP.2023.24.2.435ENEnricaSerretielloSection of Microbiology and Virology, University Hospital “Luigi Vanvitelli”, 80138 Naples, Italy.0000-0001-6556-0760FedericaCorradoExperimental Zooprophylactic
Institute of Southern, Italy.BiagioSantellaSection of Microbiology and Virology, University Hospital “Luigi Vanvitelli”, 80138 Naples, Italy.AnnalisaChianeseDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples,
Italy.DomenicoIervolinoDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.AnnachiaraCoppolaExperimental Zooprophylactic
Institute of Southern, Italy.ElenaGrimaldiSection of Microbiology and Virology, University Hospital “Luigi Vanvitelli”, 80138 Naples, Italy.MassimilianoGaldieroDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples,
Italy.GianluigiFranciDepartment of
Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi.Department of Health
Hygiene and Evaluation Medicine UOC. Clinical and Microbiological Pathology, University Hospital S. Giovanni di Dio e Ruggi
D’Aragona, Salerno Medical School, Città di Ippocrate, 84131 Salerno, Italy.0000-0003-3321-4331Journal Article20220427Introduction: Human papillomavirus (HPV) can infect both male and female genitals, skin, and mucous membranes, causing benign or malignant lesions. HPV is a common sexually transmitted infection and it is the main cause of cervical cancer. The present retrospective study updated the previously published data on HPV genotypes distribution among women living in Naples. Materials and methods: In this study, 502 cervical scrape specimens were collected from women with abnormal cytological indication and analyzed for HPV DNA identification by Linear Array HPV genotyping test. Results: The HPV infection rate was 24.1%. HPV-16 (14.6%) was the most representative HR-HPV genotypes, followed by HPV-31 (13.8%), -18 (9.2%), and HPV-51 (8.5%). In addition, HPV-42 (16.4%) was the most prevalent genotype among LR-HPV genotypes (low-risk human papillomavirus). It was also found that women at the age group of 23-29 years (42.5%) were at the highest risk of HPV infection. It was found that the HPV-16 frequency decreased, but HPV-31 and -18 frequency increased a little. The LR HPV-53 frequency decreased, leaving the first place for abundance to the LR HPV-42. HPV-6 frequency did not change. LR HPV -11 was no more present. Merging <23 and 23-29 age classes into one class followed the same result. Conclusion: HPV prevalence declined in comparison to the previous data. A frequency variation was recorded for several genotypes in this study. Data can be useful to implement the preventative strategies and to promote HPV vaccination.https://journal.waocp.org/article_90517_face0411e90a939d9a4b50ba6267ed71.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Cervical Cancer and HPV Knowledge and Awareness: An Educational Intervention among College Students in Guam4434499047810.31557/APJCP.2023.24.2.443ENLilnabeth PSomeraUniversity of Guam Cancer Research Center, United States.0000-0002-1459-049XTressaDiazDivision of Social Work, School of Health, University of Guam,
United States.0000-0002-3231-8860AngelinaMummertUniversity of Guam Cancer Research Center, United States.GrazynaBadowskiDivision of Mathematics and Computer Science, University of Guam, United States.JaeyongChoiDivision of Mathematics and Computer Science, University of Guam, United States.0000-0002-9912-0867HarmonyPalaganasUniversity of Guam Cancer Research Center, United States.KristianAysonDivision of Mathematics and Computer Science, University of Guam, United States.Journal Article20220428Background: Compared to the U.S. population, cervical cancer (CC) incidence is significantly higher among the CHamoru, Micronesian, and Caucasian populations in Guam. From 2008-2012, CC was the fifth most common cancer diagnosed on the island. Despite the prevalence of CC and low HPV vaccine uptake, there is a concerning lack of awareness and preventative behavior among young adults. This study was aimed at influencing college students’ knowledge and awareness of CC, CC screening, HPV, and the HPV vaccination. Methods: We delivered a 30-minute educational intervention to a sample of 108 university students in a classroom setting. The effect of the educational intervention was measured through a pre-and post-test on CC and HPV health behaviors, knowledge, and awareness. Results were analyzed using SPSS and an exact McNemar’s test was used to examine the difference in the proportion of correct answers to the tests. Two-way mixed ANOVA was used to examine between (gender, ethnicity, and class level) and within subjects (pre-and post-test) program effects. Results: Of the 108 participants, only 39 (36.1%) reported being vaccinated for HPV, 23 (21.3%) had not been vaccinated, and 46 (42.6%) did not know if they had been vaccinated for HPV. Only forty-one (60.3%) female participants had had a Pap smear. When comparing the pre-to-posttest responses, most questions had an increase in correct responses. Time also influenced CCA and HPV knowledge and awareness scores as there was a significant increase in scores from the pre- to post-test. Conclusion: The educational intervention was an effective tool for increasing knowledge and awareness of CC, HPV, and HPV vaccination among college students. While study results demonstrate the educational intervention’s success as a baseline measure of knowledge, the inclusion of behavioral outcome measures, such as intent to get vaccinated or screened, could result in more robust future studies.https://journal.waocp.org/article_90478_15874a56be9de1063a356f0dcc8b4262.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201P53 Gene Expression and Nitric Oxide Levels after Artemisinin-Caffeine Treatment in Breast, Lungs and Liver of DMBA-Induced Tumorigenesis4514589047610.31557/APJCP.2023.24.2.451ENTitilope MDokunmuDepartment of Biochemistry, Covenant University, Ota, Ogun State, Nigeria.0000-0002-8751-3436Sandra COparaDepartment of Biochemistry, Covenant University, Ota, Ogun State, Nigeria.Ngozi AwaImagaDepartment of Biochemistry, College of Medicine,
University of Lagos, Lagos, Nigeria.Omiete UAwaniDepartment of Biochemistry, Covenant University, Ota, Ogun State, Nigeria.David OEnomaDepartment of Biochemistry, Covenant University, Ota, Ogun State, Nigeria.Bababode IAdelaniDepartment of Biochemistry, Covenant University, Ota, Ogun State, Nigeria.Journal Article20220430Objective: With increasing incidence of cancers globally and limited resources in some affected countries, repurposing existing drugs for reducing tumorigenesis is highly important. Artemisinin and caffeine have potent anti-oxidative and anti-tumor properties but are therapies for other diseases. This study evaluated the biochemical and p53 gene modulatory effects of doses of artemisinin-caffeine combination on breast, lungs and liver tissues in rats induced with DMBA. Methods: After due ethical approval, 30 animals were treated with 40mg/kg single dose of 7,12-dimethylbenzene anthracene (DMBA) as a model for DNA damage and induction of carcinogenesis. Five animals each received normal saline (normal), low dose artemisinin (Art; 4mg/kg), low dose caffeine (Caff; 25mg/kg), low dose combination of caff + art (25+4mg/kg), high dose combination of caff + art (50+8mg/kg) or no treatment (DMBA). All treatment doses were orally administered daily for two weeks post DMBA treatment. Nitric oxide levels and p53 relative gene expression was carried out using primer-specific RT-PCR, GAPDH was used as loading control and amplicons were resolved by gel electrophoresis. Results: DMBA induced lesions in breast, liver, and lung tissues evident from histology analysis, compared to normal group. In all 3 tissues, caffeine (25mg/kg) and combination of caff + art (25+4mg/kg) significantly reduced p53 gene expression (p < 0.05), but there was significant increase in the group treated with low dose art (4mg/kg) and high dose caff + art, which were similar to DMBA group (p<0.05). In lungs, nitric oxide (NO) increased in all groups but not in caffeine, in the liver NO decreased with caffeine or its combination with art, compared to DMBA group. Conclusions: This study shows a dose-dependent synergistic anticancer effects of caffeine and artemisinin combination on p53 gene and nitric oxide regulation hence can mitigate tumor development. https://journal.waocp.org/article_90476_ceebe04f70df125e2888fea07dd0c503.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Exploring Discomfort Experienced During Chemotherapy in Thai Breast Cancer Patients4594659047910.31557/APJCP.2023.24.2.459ENParaneePhongnopakoonFaculty of Nursing, Thammasat University, Pathum Thani, Thailand.0000-0001-8818-3793BoonjaiSrisatidnarakulFaculty of Nursing, Thammasat University, Pathum Thani, Thailand.Yu YunHsuDepartment of Nursing, National Cheng Kung University,
Tainan, Taiwan.Journal Article20220502Introduction: Breast cancer is the most common cancer in females worldwide, and a new era is prevalent in the early stage. A qualitative approach explores discomfort experienced during adjuvant chemotherapy among Thai breast cancer patients. Method: The participants were selected by purposive sampling with a variation of two comprehensive cancer centers. Individual in-depth interviews were conducted with fifteen patients who had completed the second cycle of adjuvant chemotherapy prior to the interview. Results: A qualitative content analysis of data revealed two themes, six categories, and 23 sub-categories. The themes defined discomfort characteristics and factors leading to discomfort. Among all the categories were described physical discomfort, environmental discomfort, psychological discomfort, needing to relieve discomfort, lack of socio-cultural support, and lack of mental support. Conclusion: There is a need to alleviate discomfort, specifically due to Thai beliefs and culture related to patient self-management and nursing care. These findings may be extended to best practice nursing interventions to enhance comfort outcomes for breast cancer patients and elevate patient satisfaction.https://journal.waocp.org/article_90479_740778fa3cf64dab97aec63002fc4dfb.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Evaluation of LGR5 Cancer Stem Cell Marker Expression in Breast Cancer and Its Relationship with Hormonal Profile and Clinical Pathological Features4674709048010.31557/APJCP.2023.24.2.467ENFatemehMontazerDepartment of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.BehnazBoozariDepartment of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.RezaAlizadeh-NavaeiGastrointestinal Cancer Research Center, Non-communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran.0000-0003-0580-000XJournal Article20220509Background: Due to the high prevalence of breast cancer and the importance of evaluating new prognostic criteria for effective treatment of these patients, this study was performed to investigate the role of LGR5 in breast cancer and its relationship with hormonal and clinicalopathological features of the disease. Methods: This cross-sectional study was performed on breast cancer tissue samples in the archives of the pathology department of Firoozabadi Hospital in Tehran between 2019 and 2021. Inclusion criteria included invasive ductal carcinoma and exclusion criteria were preoperative chemotherapy. Blocks were examined for LGR5 marker expression by IHC method using LGR5 monoclonal antibody kits (Abcam). The expression pattern of LGR5 marker was cytoplasmic and cells presenting brown staining in the cytoplasm were considered positive for this marker and in terms of distribution and severity of staining were divided into three groups: mild, moderate and severe. Results: This study was performed on 60 patients with breast cancer with a mean age of 55.5±9.7. Most of the patients (55%) were in grade II. The KI67 marker was positive in 45 cases (75%) and the HER2 marker in 14 cases (23.3%) and 8 cases (13.3%) were triple-negative. The expression severity of staining of LGR5 marker in 41 cases (68.3%) was moderate and the distribution of marker expression in 31 cases (51.7%) was moderate. No significant relationship was observed between LGR5 expression severity and tumor characteristics. Conclusion: LGR5 marker is expressed in a remarkable percentage of breast cancer patients and has no significant relationship with tumor characteristics.https://journal.waocp.org/article_90480_e0e3bdbee605f867aff6aff2afdc7e3a.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Factors Associated with Diagnostic Delays among Tunisian Breast Cancer Patients4714779051410.31557/APJCP.2023.24.2.471ENSalmaBalhiFaculty of Medicine of Tunis, Department of Epidemiology and Public Health, Street DjabelLakhdar, 1002, Tunis, Tunisia.HyemKhiariFaculty of Medicine of Tunis, Department of Epidemiology and Public Health, Street DjabelLakhdar, 1002, Tunis, Tunisia.MohamedHsairiDepartment of Biostatistics and Epidemiology, Cancer Registry North of Tunisia, Salah Azaiz Institute, Tunis, Tunisia.Journal Article20220531Background: Breast cancer (BC) remains one of the most common and deadliest cancer among women worldwide and in Tunisia. The lethality of BC is particularly high in developing countries, such as Tunisia, due to late diagnosis in the majority of cases. The objective of this study was to analyze factors associated with delayed presentation among women followed at the Salah Azaiez institute of Tunis during the year 2021. Methods: It was a retrospective cohort study, including patients with primary BC, consulting at the Salah Azaeiz institute of Tunis over a period of six months, from january 1st to june, 30th, 2021. The total diagnostic interval (DI) was divided into patient interval (PI) and healthcare system interval (HSI). Diagnosis delay due to patient (PD) was defined as a delay more than three months between the discovery of symptoms and the first medical consultation. Data were collected using a valid questionnaire “the breast cancer questionnaire delay” and from patients’ medical records. The calculation of crude and adjusted Odds Ratios using a stepwise descending binary logistic regression model was performed to measure the association of the studied factors with the delayed presentation. Results: A total, 146 patients were included. The mean age of the patients was 50.1 ± 10.9 years. More than a half of patients had a primary education (56.8%). Majority of patients were married (86.3%) and unemployed (90.4%). The median of patient interval (PI) and HSI were 31 days [IQR: 12.5-151.5] and 53 days [IQR: 33.0-88.7] respectively. About three quarter of patients (76.4%) presented a healthcare system delay (HSD). After univariate analysis, factors significantly associated with PD were the low level of education (illiterate/primary) (RR: 2.7; 95% CI [1.1-6.4]; p=0.02) and the lack of knowledge about BC symptoms by patients (RR: 15.0; 95% CI [6.0-37.4]; p<10-3). The only variable that remained associated with PD, after multivariate analysis, was lack of initial knowledge of symptoms (OR: 15.0; 95% CI [6.0-37.4]; p<10-3). Conclusion: Lack of knowledge of symptoms was the main factor associated with delayed presentation among BC women.This study shows the need to inform women about performing breast self-examination as well as clinical signs of BC.https://journal.waocp.org/article_90514_355d61d39d9ac7f7bc3d6a639c2ce414.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Knowledge and Practice Concerning Breast Cancer Risk Factors and Screening among Females in UAE4794879048210.31557/APJCP.2023.24.2.479ENMaram OAbbasDepartment of Clinical Pharmacy and Therapeutics, Dubai Pharmacy College for Girls, Dubai, UAE.Institute of Public Health, College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates.MirzaBaigDepartment of Clinical Pharmacy and Therapeutics, Dubai Pharmacy College for Girls, Dubai, UAE.Journal Article20220614Breast cancer is considered the most dangerous cancer for women, driving the highest number of mortalities in women worldwide. According to the WHO 2020 report, breast cancer showed the highest five-year prevalence in the UAE, among other cancers. This research assessed breast cancer awareness, potential risk factors, screening approaches and practices, barriers to screening, and attitudes toward seeking medical help among UAE women. A cross-sectional community-based study was conducted through a web-based validated questionnaire. Data analysis was carried out using IBM SPSS version 27. The questionnaire was sent through social media platforms. The eligible completed were 616 responses. This study showed a prevalence of breast cancer of 3.1% among the study population. Regarding Breast cancer knowledge, most of the participants, 65.8% had moderate knowledge, 19% had poor knowledge, and only 7.6% had good knowledge. Breast cancer screening methods were the most recognized section at 76%, followed by knowledge of symptoms and while the least known section was the BC risk factors. Twenty-five percent of respondents had at least one breast cancer symptom. About 37.1% of women aged more than 40 years had never undergone mammography. In potential, most participants and 81.7% were having more than five of the BC risk factors had adequate knowledge about breast cancer with relatively higher knowledge scores for screening methods and symptoms. Participants who received information from healthcare providers or attended awareness events had a higher knowledge score. In contrast, insufficient mammography screening had been revealed. At the same time, potential risk evaluation revealed a high percentage of participants suffering from many potential risk factors.https://journal.waocp.org/article_90482_da52950d5f6f393813cd3be69dc3f6b9.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201The Direct and Indirect Costs of Cancer among the Lower-Income Group: Estimates from a Pilot and Feasibility Study4894969048310.31557/APJCP.2023.24.2.489ENFarhanaAminuddinCentre of Health Economics Research, Institute for Health Systems Research, Ministry of Health Malaysia, Shah Alam, Selangor,
Malaysia.0000-0003-3950-1534Mohd ShahriBahariCentre of Health Economics Research, Institute for Health Systems Research, Ministry of Health Malaysia, Shah Alam, Selangor,
Malaysia.Nur AzmiahZainuddinCentre of Health Policy Research, Institute for Health Systems Research, Ministry of Health Malaysia, Shah Alam,
Selangor, Malaysia.Ainul NadzihaMohd HanafiahCentre of Health Economics Research, Institute for Health Systems Research, Ministry of Health Malaysia, Shah Alam, Selangor,
Malaysia.Policy and International Relations Division, Ministry of Health Malaysia, Malaysia.Nor Zam AzihanMohd HassanCentre of Health Economics Research, Institute for Health Systems Research, Ministry of Health Malaysia, Shah Alam, Selangor,
Malaysia.Journal Article20220616Background: Healthcare in Malaysia is largely publicly funded, however, cancer could still result in out-of-pocket (OOP) expenses, which may burden the affected patients. This is especially relevant to those in the lower-income group. This pilot study was conducted to estimate the direct and indirect costs of cancer and evaluate the feasibility of obtaining these costs information from the lower-income cancer patients undergoing treatment. Methods: A cross-sectional study of patients with cancer was conducted in Hospital Kuala Lumpur between September and October 2020. Self-reported data from the patients were collected using face-to-face interviews. Detailed information about cancer-related OOP expenses including direct medical, direct non-medical, and productivity loss in addition to financial coping strategies were collected. Costs data were estimated and reported as average annual total costs per patient. Results: The mean total cost of cancer was estimated at MYR 7955.39 (US$ 1893.46) per patient per year. The direct non-medical cost was the largest contributor to the annual cost, accounting for 46.1% of the total cost. This was followed by indirect costs and direct medical costs at 36.0% and 17.9% of the total annual costs, respectively. Supplemental food and transportation costs were the major contributors to the total non-medical costs. The most frequently used financial coping strategies were savings and financial support received from relatives and friends. Conclusion: This study showed that estimation of the total cost of cancer from the patient’s perspective is feasible. Considering the significant impact of direct non-medical and indirect costs on the total costs, it is vital to conduct further exploration of its cost drivers and variations using a larger sample size. https://journal.waocp.org/article_90483_736e19fee84ab2f78ab0d8109be0d828.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Glypican-3, Vascular Endothelial Growth Factor and Golgi Protein-73 for Differentiation between Liver Cirrhosis and Hepatocellular Carcinoma4975079051510.31557/APJCP.2023.24.2.497ENSamahMamdouhDepartment of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt.0000-0001-5979-3189AmiraSolimanNational Cancer Institute,
Cairo University, Cairo, Egypt.FatmaKhorshedDepartment of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt.0000-0003-0642-1239MohamedSaberDepartment of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt.Journal Article20220620Background: Hepatocellular carcinoma (HCC) accounts for more than 80% of primary liver cancers. Moreover, in the next 10 years, more than one million patients are expected to die from liver cancer as estimated by the World Health Organization. The aim of the present study is to evaluate the clinical utility of using Glypican (GPC3), Vascular endothelial growth factor (VEGF) and Golgi protein 73 (GP73) in serum by Enzyme-Linked Immunosorbent Assay (ELISA) and by Real-Time Polymerase Chain Reaction (RT-PCR), as diagnostic markers to differentiate HCC from cirrhotic liver disease. Methods: A total of 50 patients with histologically-proven HCC, 50 liver cirrhosis patients and 20 healthy volunteers as controls were enrolled in this study, blood samples were obtained from each patient. Expression of the studied biomarkers was evaluated by ELISA and Real-Time PCR. Results: Statistical analysis of RT-PCR results showed that the expression of GPC3, VEGF and GP73 in serum of patients with HCC was significant (P value < 0.001, 0.01, and < 0.001) respectively and increased when compared to the cirrhotic group. Furthermore, the serum protein levels of GPC3 and VEGF in HCC and cirrhotic patients were significant when compared to the control group. While no significance was found between HCC and cirrhotic group. The serum protein level of GP73 was significantly increased in HCC and cirrhosis groups compared to the control group (P value < 0.001). Moreover, a significant increase was evident in HCC group compared to cirrhotic group (P value < 0.001). The results of the present study showed that the combination of VEGF and GP73 could discriminate HCC from cirrhosis. Conclusion: GPC3, VEGF and GP73 are reliable biomarkers for diagnosis of HCC. The serum level of GP73 is a potential screening marker for discriminating HCC from liver cirrhosis. https://journal.waocp.org/article_90515_edfa95e2d6d6dc808ddb5a7363c40a5f.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Comparison of GATA-3, Mammaglobin and GCDFP-15 Expression in Primary, Metastatic and Triple Negative Breast Carcinomas: An Indian Scenario5095159048410.31557/APJCP.2023.24.2.509ENMeenakshiRaoDepartment of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0003-1310-2578ShalakaKhadeDepartment of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0002-9531-8155RamkaranChaudharyDepartment of
General Surgery, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0002-4861-5106PratibhaSinghDepartment of Obstetrics and Gynaecology, All
India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0001-5286-3826GarimaYadavDepartment of Obstetrics and Gynaecology, All
India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0002-1268-6973PoonamElhenceDepartment of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0002-4660-389XAasmaNalwaDepartment of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0002-2085-2011RashimSharmaDepartment of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0003-4748-3049Akhil DhaneshGoelDepartment of Community Medicine and Family Medicine, All India
Institute of Medical Sciences (AIIMS), Jodhpur, India.0000-0002-6156-7903Journal Article20220621Background and objective: Mammaglobin and GCDFP-15 are traditional immunohistochemistry (IHC) markers utilized to recognize metastasis of breast carcinoma in an unknown primary. GATA-3 is increasingly being used as a marker of primary breast origin. This study was done to evaluate and compare GATA-3 with GCDFP-15 and Mammaglobin in invasive primary including metastatic and triple negative breast carcinomas. Methods: Immunohistochemistry for GATA-3, GCDFP-15 and Mammaglobin was applied on 100 cases of primary breast carcinomas, including 20 triple negative cases and 30 cases of metastatic breast carcinomas. Staining scores were given for each marker by multiplying the percentage of positive tumor cells by the intensity of staining (1+, 2+ or 3+), with scores ranging from 0 to 300. Staining score of 1 or more was considered positive. Results: GATA-3 was expressed in 92% of primary, 80% of metastatic and 60% of triple negative breast carcinomas, with an average staining score of 270. Mammaglobin was expressed in 68% of primary, 56.6% of metastatic and 25% of triple negative breast carcinomas, with an average staining score of 180. GCDFP-15 was expressed in 48% of primary, 26.6% of metastatic and 05% of breast carcinomas, with an average staining score of 60. GATA-3 demonstrated to have higher staining score (average of 270) than other two markers in maximum number of cases. Conclusion: GATA-3 has a higher sensitivity and increased staining scores in primary breast carcinomas, metastatic breast carcinomas as well as in triple negative breast carcinomas.https://journal.waocp.org/article_90484_3c3113a79338216ff51e1db108eaa7c7.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Trimodality Therapy in which Concurrent Chemoradiation with Concomitant Boost in Muscle Invasive TCC Urinary Bladder Cancer5175249051810.31557/APJCP.2023.24.2.517ENAyatallah AliYoussief MohammedLecturer of Radiotherapy and Nuclear Medicine, South Egypt Cancer Institute, Assiut University, Egypt.0000-0003-3834-4151Doaa AliGamalDepartment of
Clinical Oncology, Assiut University Hospital, Egypt.0000-0002-8318-1884Ahmed RefaatAbd ElzaherDepartment of Medical Oncology, South Egypt Cancer Institute Assiut
University, Egypt.AsmaaHasaballahLecturer of Radiotherapy and Nuclear Medicine, South Egypt Cancer Institute, Assiut University, Egypt.Journal Article20220715Background: Bladder cancer is the most common genitourinary tract malignancy among Egyptian males (16%). bladder sparing therapy can be considered an alternative for patients refusing surgery or are not candidates for surgery. The objective of this study was to determine the safety and feasibility of external-beam irradiation with concomitant boost in muscle invasive bladder cancer and to determine the short-term (1-year) risk of recurrence of bladder cancer. Methods: Between October 2019 and November 2021, we enrolled 42 patients in Prospective, one arm trial. Eligible patients had pathologically confirmed TCC transitional cell carcinoma of the bladder cT2-4aN0M0, who refused surgery or had contraindications to surgery, and treated conservatively with radiotherapy. All patients underwent maximal TURB before beginning of chemoradiation therapy which was delivered in all patients The patients received radiotherapy dose 45 Gy/25 fractions (1.8 Gy) per fraction to the whole bladder+ 3 cm. with concurrent cisplatin 20 mg/ m2 over 30 minutes before radiation on days 1,2,15,16,29, and 30. Additionally, concomitant boost limited to the bladder plus1.5 cm margin was deliverd during the last ten days of the treatment with a minimum 6 h gap between fractions, to a total dose 60 Gy, with the overall treatment time equal to 5 weeks. Results: The median overall survival OS for 42 patients with transitional cell carcinoma( TCC) of bladder treated with 3D conformal radiotherapy 3DCRT and concomitant boost was 28 months, the mean OS was 29.9±1.04, and (95% confidence interval=27.9-32). The one-year OS was 100%, 2-year OS was 81%, and 3-year OS was 26.2%.The mean loco-regional relapse free survival (LRRFS) was 31.6±1.8, 95% CI=28.1-35.1, and the median was 26.5±1.4, the one-year loco-regional RFS was 92.9%, and 2-year (LRRFS) was 66.7%.Acute and late genitourinary toxicity was grade 2 in most of patients and also acute and late toxicity of gastrointestinal was equal or less than grade 1. Conclusion: In external radiotherapy for muscle invasive bladder cancer a concomitant boost technique of invasive bladder cancer with shortening of the overall treatment time provides a high probability of local control and overall survival with acceptable toxicity. https://journal.waocp.org/article_90518_3b34c9351023c4a1c1575366cc3878a2.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Prevalence of Pathogenic Germline Mutations in 13 Hereditary Cancer-Related Genes in Breast Cancer Patients in Narathiwat Province, Thailand5255309048610.31557/APJCP.2023.24.2.525ENPanupongSukpanDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai,
Songkhla, Thailand.Medical Education Center, Naradhiwas Rajanagarindra Hospital, Narathiwas, Thailand.0000000333812980KanyanattKanokwiroonDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai,
Songkhla, Thailand.HutchaSriplungDepartment of
Epidemiology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.WisonLaochareonsukDepartment of Surgery, Faculty
of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.Translational Medicine Research Center, Faculty of
Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.PongsakornChoochuenDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai,
Songkhla, Thailand.Translational Medicine Research Center, Faculty of
Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.NasuhaAusengMedical Education Center, Naradhiwas Rajanagarindra Hospital, Narathiwas, Thailand.KasemsunWanawanakornDepartment of Primary Care, Su-ghai Kolok Hospital,
Narathiwat, Thailand.SurasakSangkhathatDepartment of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai,
Songkhla, Thailand.Translational Medicine Research Center, Faculty of
Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.Journal Article20220716Background: BRCA1 and BRCA2 genes are known to increase breast cancer’s lifetime risk. Early identification of women with this inherited risk can potentially reduce the risk of breast and/or ovarian cancer and, together with early screening, decrease the mortality rate. Objective: This study explored the frequency and distribution of genetic variants in consecutive cases of breast cancer in Narathiwat province, one of the three provinces in the southernmost Thai border. Material & Method: A series of 64 consecutive breast cancer patients who underwent treatment in two general hospitals in the province during the period from the year 2021 to 2022. Genotyping studies were performed using a whole exome sequencing platform. Moderate to high penetrance variants recommended by the National Comprehensive Cancer Network (NCCN) guidelines 2022 (ATM, BARD1, BRCA1, BRCA2, CDH1, CHEK2, NF1, PALB2, PTEN, RAD51C, RAD51D, STK11, TP53) were annotated and filtered for pathogenic, likely pathogenic, or high-impact variants. Results: Pathogenic germline variants were found in 8/64 cases (12.5%), namely BRCA1 in 3 (4.7%), BRCA2 in 4 (6.3%), ATM in 1 (1.6%), and PALB2 in 1 (1.6%). One patient had two concomitant germline mutations in BRCA2 and ATM. Conclusion: This is the first study on the frequency of germline mutations in BRCA1/2 and other breast cancer-predisposing genes in the southernmost provinces of Thailand. At least one pathogenic germline mutation was identified in 12.5% of the study patients, which suggests that genetic testing in this population has a high potential to provide benefits.https://journal.waocp.org/article_90486_c11655e35a88cdec86407da0a33d5960.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Convolution Neural Network for Breast Cancer Detection and Classification Using Deep Learning5315449048710.31557/APJCP.2023.24.2.531ENBasem SAbunasserUniversity Malaysia of Computer Science & Engineering (UNIMY), Cyberjaya, Malaysia.Mohammed Rasheed J.AL-HiealyUniversity Malaysia of Computer Science & Engineering (UNIMY), Cyberjaya, Malaysia.Ihab SZaqoutFaculty of Engineering and Information
Technology, Al-Azhar University, Gaza, Palestine.Samy SAbu-NaserFaculty of Engineering and Information
Technology, Al-Azhar University, Gaza, Palestine.0000-0002-7677-8279Journal Article20220722Objective: Early detection and precise diagnosis of breast cancer (BC) plays an essential part in enhancing the diagnosis and improving the breast cancer survival rate of patients from 30 to 50%. Through the advances of technology in healthcare, deep learning takes a significant role in handling and inspecting a great number of X-ray, MRI, CTR images. The aim of this study is to propose a deep learning model (BCCNN) to detect and classify breast cancers into eight classes: benign adenosis (BA), benign fibroadenoma (BF), benign phyllodes tumor (BPT), benign tubular adenoma (BTA), malignant ductal carcinoma (MDC), malignant lobular carcinoma (MLC), malignant mucinous carcinoma (MMC), and malignant papillary carcinoma (MPC). Methods: Breast cancer MRI images were classified into BA, BF, BPT, BTA, MDC, MLC, MMC, and MPC using a proposed Deep Learning model with additional 5 fine-tuned Deep learning models consisting of Xception, InceptionV3, VGG16, MobileNet and ResNet50 trained on ImageNet database. The dataset was collected from Kaggle depository for breast cancer detection and classification. That Dataset was boosted using GAN technique. The images in the dataset have 4 magnifications (40X, 100X, 200X, 400X, and Complete Dataset). Thus we evaluated the proposed Deep Learning model and 5 pre-trained models using each dataset individually. That means we carried out a total of 30 experiments. The measurement that was used in the evaluation of all models includes: F1-score, recall, precision, accuracy. Results: The classification F1-score accuracies of Xception, InceptionV3, ResNet50, VGG16, MobileNet, and Proposed Model (BCCNN) were 97.54%, 95.33%, 98.14%, 97.67%, 93.98%, and 98.28%, respectively. Conclusion: Dataset Boosting, preprocessing and balancing played a good role in enhancing the detection and classification of breast cancer of the proposed model (BCCNN) and the fine-tuned pre-trained models’ accuracies greatly. The best accuracies were attained when the 400X magnification of the MRI images due to their high images resolution.https://journal.waocp.org/article_90487_a6b01133b91d4b89b6c6c1ad3dbfeb57.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Retrospective Analysis of Clinical Outcomes of Stereotactic Body Radiation Therapy for Localized Prostate Cancer at an Asian Cancer Specialist Centre5455509048510.31557/APJCP.2023.24.2.545ENJasmin LohPei YuinDepartment of Oncology, Beacon Hospital, Petaling Jaya, 46050 Selangor, Malaysia.Jessica TanJia ShinClinical Research Department, Beacon
Hospital, Petaling Jaya, 46050 Selangor, Malaysia.Chen BaoJingClinical Research Department, Beacon
Hospital, Petaling Jaya, 46050 Selangor, Malaysia.Tho LyeMunDepartment of Oncology, Beacon Hospital, Petaling Jaya, 46050 Selangor, Malaysia.Manivannan ArkonamBalasubramaniamDepartment of Oncology, Beacon Hospital, Petaling Jaya, 46050 Selangor, Malaysia.Dato MohamedIbrahim WahidDepartment of Oncology, Beacon Hospital, Petaling Jaya, 46050 Selangor, Malaysia.Journal Article20220801Introduction: The current treatment options for localized prostate cancer are radical prostatectomy and external beam radiotherapy (EBRT) with stereotactic body radiation therapy (SBRT) gaining interest as a treatment option compared to standard fractionation radiation therapy. This present study is a retrospective study evaluating the correlations between the biochemical efficacy, and treatment toxicity in SBRT for localized prostate cancer. Methods: All organ-confined prostate cancer patients treated with SBRT from 2010 to 2018, at Beacon Hospital, Malaysia were included in this study. Patient demographics, dosimetric parameters, and disease information were retrospectively collected. The primary endpoint was biochemical recurrence-free survival assessed using the Phoenix definition (Nadir + 2 ng/mL). Toxicity outcomes were scored using the Radiation Therapy Oncology Group scale. Results: Fourty-nine patients who met the inclusion criteria (5 low-, 13 intermediate- and 31 high-risk according to the D’amico Risk Classification) received SBRT. The most common dose regime was 34-35Gy in 5 fractions (n=18). Other dose regimes were 24Gy in 3 fractions and 25-33Gy in 5 fractions. Median follow-up was 45.4 months. The median pre-treatment prostate-specific antigen (PSA) was 11.22 ng/mL, which decreased to a median PSA of 0.1 ng/mL by 2 years post-treatment. Out of the 49 cases, only 1 case of biochemical recurrence occurred, yielding a 3- and 5-year overall survival of 100%, and a 3- and 5- year biochemical recurrence-free rate of 100% and 95.2%. Acute grade III urinary toxicities occurred in 1 (2%); whereas acute grade I urinary and rectal toxicities were seen in 22 (44.9%) and 7 (14.3%) patients respectively. Grade I and grade III late rectal toxicities occurred in 3 and 1 patients respectively, while 3 and 1 patient reported late grade I and III urethral stricture respectively. Conclusion: SBRT for clinically-localized and locally advanced prostate cancer provided promising outcomes with low toxicity and good biochemical control.https://journal.waocp.org/article_90485_672971481214ae6ce91cfb798018f646.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Atractylodin and β-eudesmol from Atractylodes lancea (Thunb.) DC. Inhibit Cholangiocarcinoma Cell Proliferation by Downregulating the Notch Signaling Pathway5515589048810.31557/APJCP.2023.24.2.551ENPeeranateVanarojGraduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Paholyothin
Road, Klonglung, Pathumthani 12120, Thailand.WannaChaijaroenkulGraduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Paholyothin
Road, Klonglung, Pathumthani 12120, Thailand.0000-0002-3720-3878KesaraNa-BangchangGraduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Paholyothin
Road, Klonglung, Pathumthani 12120, Thailand.Center of Excellence in Pharmacology and Molecular Biology of Malaria and
Cholangiocarcinoma, Thammasat University, Paholyothin Road, Klonglung, Pathumthani 12120, Thailand.0000-0001-6389-0897Journal Article20220808Objective: Notch signaling pathway has been reported to be involved in the development and progression of various types of cancer, including cholangiocarcinoma (CCA). Compounds that modulate this signaling pathway could be promising candidates for CCA treatment and control. The study investigated the antiproliferative activities and modulatory effects of atractylodin and β-eudesmol, the two bioactive compounds of Atractylodes lancea (Thunb.) DC. , on Notch signaling and upstream molecules (Notch1 and Notch2 receptors, JAG1, mTOR, PI3K, and YAP), and downstream molecules (Snail) in HuCCT-1 (CCA cell line) and OUMS-36T-1 (normal fibroblast cell line). Gemcitabine (standard drug for CCA), and Notch inhibitors (DAPT and zebularine) were included in the experiments as positive control compounds. Methods: The antiproliferative activity was evaluated using MTT assay. mRNA and protein expression of Notch signaling molecules were evaluated using real-time PCR and Western blot analysis. Results: Atractylodin and β-eudesmol moderately inhibited HuCCT-1 cell growth with IC50 (concentration that inhibits cell growth by 50%) of 29.00 ± 6.44 and 16.80 ± 4.41 µg/ml (mean±SD), respectively. The direction and extent of the modulatory effects on mRNA and protein expression in the CCA cell line varied with the signaling molecules. Notch1 receptor was shown to be the most promising target of atractylodin and β-eudesmol in CCA. The level of gene expression was significantly downregulated (0.042 to 0.195 fold of control) after treating HuCC-T1 cells with both compounds at low and high concentrations. The extent and change in Notch1 gene expression correlated well with protein expression. Conclusion: The notch signaling pathway could be a promising target of atractylodin and β-eudesmol in CCA. <br /> https://journal.waocp.org/article_90488_e1061f4fe05d18f22f7c603faf54f876.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Factors Associated with Knowledge and Attitude towards E-Cigarettes among Undergraduate Students in Thailand: A Cross-Sectional Study5595679051910.31557/APJCP.2023.24.2.559ENTiwaphonThongsuttDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.ChawisaYusoteDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.SornnapatJubprangDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.ApisaraSasisuwanDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.NatnichaPoonchuayDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.ApichayaChanawongDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.TidaSottiyotinDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.SawitreeLaopaiboonkunDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.Fares M.SMuthannaDepartment of Pharmaceutical Care, School of Pharmacy, Walailak University, Nakhon Si Thammarat, 80160, Thailand.0000-0002-7722-9466Journal Article20220813Background: Thailand government has enacted a ban on vaping for eight years, but the number of vapers has continued to increase, particularly among teenagers. This study aimed to evaluate undergraduate pharmacy students’ knowledge and attitudes toward e-cigarette use in Thailand. Method: This descriptive cross-sectional study was performed between January and March 2022. The participants were selected from 14 universities of Thailand. For data collection, a questionnaire was developed online using Google Form. The participants had to complete the questionnaire within 5 to 10 days the via s. Chi-square and binary logistic analysis were used to assess the association between variables. Results: A total of 507 participants were recruited, The participants’ mean age was 20.6 ± 1.9 years. About 98.6% of the participants knew about e-cigarette, and 74.4% had never been taught about e-cigarettes. Moreover, 68.8% of the participants declared that people around them were smokers. Logistic regression analysis indicated that the participants who declared that they were not sure if e-cigarettes contains nicotine or not were 10.5 more likely to consume e-cigarettes (AOR = 10.5; 95 % CI 3.130-35.181; P ˂0.001). Male participants who were at the academic year of three and four were more likely to use e-cigarettes than female students who were at the academic year of five and six (AOR 2.9; 95 % CI 1.599-5.214; P ˂0.001; AOR = 4.5; 95 % CI 1.412-14.571; P ˂0.001; AOR = 3.9; 95 % CI 1.263-12.511; p = 0.018). Conclusion: OOur study findings showed that pharmacy students lacked knowledge about e-cigarettes and misunderstood about e-cigarettes substances. Future research is necessary to develop educational programs for health professionals to be ready to advise patients about e-cigarettes.https://journal.waocp.org/article_90519_115adb15544cf7f59dbdf63164e7660c.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201The Efficacy of Psychoeducational Support Group for Saudi Breast Cancer Survivors5695749049010.31557/APJCP.2023.24.2.569ENHaifa AAlshamsiPsych Care Clinic, Riyadh, Saudi Arabia.Hala AAseelPsych Care Clinic, Riyadh, Saudi Arabia.Dalia AObeidDepartment of Infectiouse Genome, Public Health Authority, Riyadh, Saudi Arabia.0000-0003-0251-1894AmaniAljohiPhysical Medicine and Rehabilitation, King Fahad Medical City KFMC, Riyadh, Saudi Arabia.Journal Article20220814Background: The adverse effects of psychological distress and quality of life are common outcomes in breast cancer survivors. Psychoeducational interventions have been widely used to reduce these adverse outcomes among those patients. This study aims to assess the impact of the Blossom support group on psychological distress and quality of life in breast cancer patients in Saudi Arabia. Methods: This study is a retrospective cohort study for the Blossom program, which was held yearly for eight weeks from 2014 until 2018. Data was collected from the survivors’ records at the Zahra Breast Cancer Association in Riyadh, Saudi Arabia. Outcome measurements: include the quality of life scale (SF-36) and Hospital Anxiety Depression Scale (HADS) with both were available in the Arabic translated version. Both tools were used as the screening measurement for the assessment of the quality of life, depression, and anxiety before and after the program. The data were analyzed using the Statistical Analytical Software (SAS), version 9.4. Statistical differences in median scores of SF-36 and HADS before and after the program was tested using Wilcoxon signed-rank test. Result: A total of 54 breast cancer survivors were enrolled in the Blossom support program over the period 2014 to 2018. Of these participants, only 25 participants completed the full eight weeks program. There were statistically significant differences in the overall quality of life (SF-36) before and after the 8 weeks from baseline of the experiment (W = 60, P-value =0.0125). Moreover, the overall anxiety and depression scores were tested separately and the results reveal statistically significant differences in both dimensions before and after the program (Anxiety: W=132, P-value = 0.0059; depression: W=157, P-value = 0.0002). Conclusion: The Blossom support program showed a positive impact on the quality of life and the psychological well-being of breast cancer survivors. It is recommended to utilize this support program across a wide range of health organizations with breast cancer survivors as a baseline of psychological care to improve the quality of life. https://journal.waocp.org/article_90490_a6fdafe96071dbf629a172b35437dab1.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Predictive Factors for Post-Hepatectomy Liver Failure in Patients with Cholangiocarcinoma5755809049310.31557/APJCP.2023.24.2.575ENWanthiwaKriengkraiA Student of the Master of Nursing Science (Adult Nursing), Faculty of Nursing, Khon Kaen University, Khon Kaen, Thailand.BusabaSomjaivongDepartment of Adult Nursing, Faculty of Nursing, Khon Kaen University, Khon Kaen, Thailand.0000-0002-6399-8651AttapolTitapunDepartment of Medicine,
Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.ParichatWonggomDepartment of Adult Nursing, Faculty of Nursing, Khon Kaen
University, Khon Kaen, Thailand.Journal Article20220814Background: Cholangiocarcinoma (CCA) is a cancer of biliary tract with a different incidence across the globe. The northeast part of Thailand has the highest incidence of CCA. Post-hepatectomy liver failure (PHLF) is the most severe and life-threatening complication in CCA patients. The aim of this study was to identify factors related to PHLF incidence in patients with CCA. Methods: An analytical cross-sectional study was performed in a university hospital in northeast Thailand between January 1, 2014 and December 31, 2020. PHLF grading criteria was used according to the international study group of liver surgery definition for PHLF. Multiple Logistic Regression with a stepwise forward method was employed to identify the predictive factors related to PHLF. Result: The study findings revealed that 185 patients developed PHLF, of whom 56.22% experience grade A, 36.76% grade B, and 7.06% grade C PHLF. Based on our findings, seventeen factors were significantly correlated with PHLF incidence, namely age, cholangiocarcinoma type, hepatectomy type, preoperative biliary drainage, cholangitis, Child-Turcotte-Pugh grade, operation time, total blood loss, total blood transfusion, level of serum albumin, total bilirubin, direct bilirubin, alanine transaminase, aspartate transaminase, alkaline phosphatase, prothrombin time, and level of international normalized ratio. Total bilirubin (adjusted OR=14.07, 95% CI 7.54-26.27), Child-Turcotte-Pugh grade (adjusted OR=3.34, 95% CI 1.43-7.81), total blood transfusion (adjusted OR=2.32, 95% CI 1.19-4.54), and operation time (adjusted OR=1.77, 95% CI 1.05-2.97) could significantly predict PHLF incidence with a positive predictive value of 86.03% and a negative predictive value of 80.23%, while the accuracy of prediction was 81.88%. Conclusion: The findings of this study identified total bilirubin, Child-Turcotte-Pugh grade, total blood transfusion, and operation time as clinical predictive factors of PHLF. Therefore, modification of these factors is recommended to reduce the probability of liver failure in CCA patients.https://journal.waocp.org/article_90493_7afa9e13df566a3cf0b98328b9295294.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Cytotoxic Activity of the Ethyl Acetate Extract of Iraqi Carica papaya Leaves in Breast and Lung Cancer Cell Lines5815869049110.31557/APJCP.2023.24.2.581ENSuraBasimDepartment of Pharmacognosy, College of Pharmacy, University of Baghdad, Baghdad, Iraq.Ali AKasimDepartment of Pharmacognosy and Medicinal Plants, Department of Pharmacognosy, College of Pharmacy, University of Baghdad, Iraq.Journal Article20220905The aim of this study was to evaluate the cytotoxic effect of the ethyl acetate fraction of Iraqi Carica papaya (C. papaya) in breast and lung cancer cell lines, MCF-7 and A549, respectively. Methods: The ethyl acetate extract of Iraqi C. papaya leaves was prepared and tested for its phytochemical constitution. The 3-(4,5-dimethylthiazoline-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed in breast (MCF-7) and lung (A549) cells lines that were treated with different concentrations of ethyl acetate extract (3.125,6.25,12.5, 25, 50, and 100μg/ml). After 72 hrs of treatment, cell viability was evaluated. Results: The ethyl acetate extract of C. papaya showed considerable cytotoxic activity in the MCF-7 and A549 cell lines. The activity was dose-dependent; The half-maximum inhibitory concentration (IC50) values were 22.74μg/ml and 8.674 μg/ml in the MCF-7 and A549 cell lines, respectively. Conclusion : The ethyl acetate fraction of Iraqi C. papaya leaves has potential anticancer activity in lung and breast cancer.https://journal.waocp.org/article_90491_069ec139d0cf2453463bed3e9ce4929f.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Effectiveness of Symptom Management Intervention for Improving the QOL of Cancer Patients5875969049210.31557/APJCP.2023.24.2.587ENMalathi GNayakDepartment of Community Health Nursing, Manipal College of Nursing, Manipal Academy Higher Education, Manipal, Karnataka, India.0000-0002-3487-0771AniceGeorgeDepartment of Child Health Nursing, Manipal College of Nursing, Manipal Academy of Higher Education, Manipal, Karnataka, India.0000-0003-2409-696Journal Article20220914Background: Managing the symptoms of cancer patients is challenging for health care providers and interventions individually designed are required to improve the quality of life (QOL) of cancer patients. Objective: to assess the efficacy of symptom management intervention on symptom reduction and the QOL of cancer patients. Methods: A Quasi-experimental study using pre and post-test design was conducted among 200 cancer patients selected by a convenient sampling technique from the selected cancer hospitals. The intervention group received symptom management intervention and the control group received routine clinical care. The data were collected from individuals who had been diagnosed with breast/head and neck cancer and were in the third or fourth stages of cancer, using symptom assessment and Functional Assessment of Cancer Therapy (FACT) QOL tool. After the pre-test, symptom management intervention was provided, and a post-test was conducted at one month and three months after the intervention. Results: The mean age was 50.93 years among the participants. Fifty-two percent and 68% of them were in stage IV cancer in the intervention group and control group respectively. The mean QOL score of head and Neck cancers in the intervention group increased from 20.76 (1.82) to 97.03 (3.33) and the mean scores of QOL of breast cancer patients in the intervention group increased from 22.44 (2.92) to 94.39 (8.30). Repeated measure ANOVA showed that the intervention program was effective in enhancing symptom reduction and QOL among cancer patients (Head and Neck cancers F(1.3, 114) =391.62, p< 0.001 and Breast cancer F (1, 75) =177.41.41, p=.001). Conclusion: Nurses play a vital role in providing care to cancer patients and improving their quality of life since nurses are more involved in care.https://journal.waocp.org/article_90492_90983966d641a9283b2274e011e0519a.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Prospective Comparison of Hepatocellular Carcinoma Behavior and Survival of Patients who Did or Did not Receive HCV Direct-Acting Antivirals5976059049610.31557/APJCP.2023.24.2.597ENMohamedEl-KassasEndemic Medicine Department, Faculty of Medicine, Helwan University, Cairo, Egypt.0000-0002-3396-6894HamdySayedEndemic Medicine Department, Faculty of Medicine, Helwan University, Cairo, Egypt.DaliaOmranEndemic Medicine and Hepatology
Department, Faculty of Medicine, Cairo University, Cairo, Egypt.Ali HusseinEldahroutyGastroenterology, Hepatology, and Endemic Medicine
Department, Faculty of Medicine, Minia University, Minia, Egypt.TamerElbazEndemic Medicine and Hepatology
Department, Faculty of Medicine, Cairo University, Cairo, Egypt.EnasKamalGastroenterology, Hepatology, and Endemic Medicine
Department, Faculty of Medicine, Minia University, Minia, Egypt.Journal Article20220918Background & Aims: The safety and efficacy of hepatitis C (HCV) direct-acting antivirals (DAAs) have been established in several real-world trials; however, some reports have claimed an association between DAAs and hepatocellular carcinoma (HCC) occurrence or aggressive behavior. We aimed to prospectively examine differences in de-novo HCC tumor behavior and overall survival (OS) in DAAs-treated versus HCV-untreated patients as measured by BCLC progression during a two-year follow-up period. Methods: This multicenter cohort study recruited 523 patients with de-novo HCV-related HCC. After exclusion criteria were applied, 353 patients were placed into; Group 1, including 236 patients without a history of DAAs therapy, and Group 2 including 117 patients with de-novo HCC developed after receiving DAAs. Patients were then stratified in each group according to BCLC staging (Liver, 2018). All patients received standard of care management and were followed until death or a maximum of 2 years. Results: No statistically significant differences were observed between the two groups regarding tumor characteristics (number and size of lesions) and criteria for aggressiveness upon presentation. Among all BCLC stages, DAAs treated patients showed significantly lower baseline Fib4 values than DAA untreated patients in BCLC-0 stage (4.1 vs 7.7, p 0.019). No statistically significant differences were evident in HCC progression in the different BCLC stages at 12 and 24 months follow up periods (p 0.0718 and 0.279 respectively). Significantly better survival was recorded in Group 1 compared to Group 2 patients for BCLC stages C and D (p = 0.003 and 0.01, respectively). Conclusion: HCC may develop at an earlier stage of liver disease after DAAs therapy. No defensive role was found for DAAs treatment in delaying HCC progression that occurs after viral eradication.<br /> https://journal.waocp.org/article_90496_dc54b53c5fa8772c1f6af54d7f427ed9.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Acceptability and Preference for Human Papilloma Virus Self-Sampling among Thai Women Attending National Cancer Institute6076129049510.31557/APJCP.2023.24.2.607ENPattamaPloysawangDivision of Policy Development and Strategic Plan, National Cancer Institute, Department of Medical Services, Ministry of Public
Health, Bangkok, Thailand.SupakornPitakkarnkulDivision of Gynecologic Oncology, National Cancer Institute, Department of Medical Services,
Ministry of Public Health, Bangkok, Thailand.WarangkanaKolakaDivision of Gynecologic Oncology, National Cancer Institute, Department of Medical Services,
Ministry of Public Health, Bangkok, Thailand.PanwadRatanasrithongDivision of Gynecologic Oncology, National Cancer Institute, Department of Medical Services,
Ministry of Public Health, Bangkok, Thailand.UraiwanKhomphaiboonkijDivision of Gynecologic Oncology, National Cancer Institute, Department of Medical Services,
Ministry of Public Health, Bangkok, Thailand.ChontichaTipmedDivision of Policy Development and Strategic Plan, National Cancer Institute, Department of Medical Services, Ministry of Public
Health, Bangkok, Thailand.KandaSeedaDivision of Policy Development and Strategic Plan, National Cancer Institute, Department of Medical Services, Ministry of Public
Health, Bangkok, Thailand.ParindaPangmuangDivision of Policy Development and Strategic Plan, National Cancer Institute, Department of Medical Services, Ministry of Public
Health, Bangkok, Thailand.SuleepornSangrajrangDivision of Policy Development and Strategic Plan, National Cancer Institute, Department of Medical Services, Ministry of Public
Health, Bangkok, Thailand.Journal Article20220919Background: Cervical cancer screening is an important public health strategy to reduce cervical cancer incidence and mortality. Human papillomavirus (HPV) self-sampling is the alternative method that can potentially increase participation in cervical cancer screening. This study aimed to evaluate the acceptability of HPV self-sampling as a primary cervical cancer screening among Thai women. Methods: A cross-sectional study was conducted at National Cancer Institute, Thailand, between March and September 2021. Eligible women were invited to collect their own samples with a vaginal cotton swab for cervical screening. The data on demographics, acceptability, and preference for HPV self-sampling were collected via a self-administered questionnaire. A Likert scale was used to assess the response of self-sampling acceptability. The multivariable logistic regression determined factors that influence preference for HPV self-sampling. Results: A total of 265 participants were recruited. Over 70% agreed that self-sampling was easy, less embarrassing, and not painful. They also felt confident in their ability to self-sample correctly and would recommend this method to a friend or relative. For their next screening round, 66.4% preferred self-sampling whereas 33.6% preferred clinician-collected samples as routine screening. The factors that influence preference for self-sampling were age, marital status, feeling less embarrassed, and confidence in performing the tests. Conclusions: Most of the study participants accepted HPV self-sampling. This suggests that the self-sampling method will be an additional option to increase cervical cancer screening coverage which leads to improving the effectiveness of the national program.https://journal.waocp.org/article_90495_a31a41f99e1a3e054d272d294a0d6722.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201The Relationship between Gene Polymorphisms od the XRCC1 and TP53 with the Gender of Children with Acute Leukemia6136219049710.31557/APJCP.2023.24.2.613ENAselUsenovaDepartment of Special Surgical Disciplines, International Higher School of Medicine, 720054, 1F Intergelpo, Bishkek, Kyrgyz
Republic.StalbekAkhunbaevDepartment of Special Surgical Disciplines, International Higher School of Medicine, 720054, 1F Intergelpo, Bishkek, Kyrgyz
Republic.ArstanbekTumanbaevDepartment of Surgery, National Center of Oncology and Hematology, 720020, 92 Akhunbaev Str., Bishkek, Kyrgyz
Republic.EvgeniyaLimDepartment of Special Surgical Disciplines, International Higher School of Medicine, 720054, 1F Intergelpo, Bishkek, Kyrgyz
Republic.Journal Article20220920Background: The relevance of the study lies in the fact that although the role of polymorphism of some genes that are responsible for cell apoptosis and deoxyribonucleic acid repair in the development of acute leukemia has already been established, its relationship with the gender of patients has not been studied enough. This study was aimed at studying the relationship between the Arg399Gln polymorphism in the XRCC1 deoxyribonucleic acid repair gene and the Arg72Pro polymorphism in the TP53 tumor suppressor gene encoding the p53 protein with the gender of children with acute leukemia. Material and methods: The study included 100 newly diagnosed pediatric patients of Kyrgyz nationality (69 boys and 31 girls), among which there were 77 patients with acute lymphoblastic leukemia, 22 patients with acute myeloblastic leukemia and 1 patient with a biphenotypic variant. Determination of polymorphisms was carried out by PCR-RFLP analysis or polymerase chain reaction followed by an analysis of restriction fragment length polymorphism. The interrelation of the results obtained with the patients’ gender was assessed using statistical methods. Results: The study showed that there were no gender differences for all three genotypes of the Arg72Pro polymorphic marker of the tumor suppressor p53 (ТР53). Three Arg399Gln genotypes of the XRCC1 gene also did not depend on gender. However, with a separate analysis of each polymorphism, there was a tendency for a greater proportion of the Arg/Gln genotype in the group of boys compared to girls. The Gln/Gln polymorphism relationship requires further study due to insufficient data for analysis. Conclusion: The study has expanded the understanding of genetic changes and their relationship with gender, which have diagnostic, prognostic and therapeutic implications in acute leukemia. The conducted research of the relationship between individual phenotypes of acute lymphoblastic leukemia with risky polymorphisms in some genes contributes to the study of AL.https://journal.waocp.org/article_90497_fd587a3df177f1708d36a3c11c0aafd2.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201The Outcomes of Diffuse Large B-cell Lymphoma Patients with Synchronous and Early Central Nervous System Involvement: A Single-Center Experience6236319049810.31557/APJCP.2023.24.2.623ENMubarakAl-MansourAdult Medical Oncology, Princess Noorah Oncology Center, King Abdulaziz Medical City, Ministry of National Guard - Health Affairs, Jeddah, Kingdom of Saudi Arabia.College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.Saif AhmedSaifCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.ZiyadAlharbiCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.Abdulrhman SalemAlhwaityCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.AhmedAlmasrahiCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.WaleedAlnejadiCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.SarahHussainCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.Syed SameerAgaCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.0000-0002-8186-1149Muhammad AnwarKhanCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.RoulaAlmuftiCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.AhmedAbsiCollege of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdullah International Medical Research Center, Ministry of National Guard - Health Affairs, Jeddah, Saudi Arabia.Journal Article20220922Background: Diffuse large B cell lymphoma (DLBCL) is the most commonly diagnosed subtype of non-Hodgkin’s lymphoma (NHL). R-CHOP has significantly improved clinical outcomes in patients with DLBCL, however, its indication in the prevention of CNS relapse and recurrence is still inconsistent. Moreover, prophylactic methotrexate and/or cytarabine have been used prophylactically for DLBCL patients is at high risk of CNS relapse and to treat CNS DLBCL, however, their efficacy remains unclear. Methods: The aim of our retrospective study was to determine the incidence of CNS in-volvement in patients with DLBCL and to describe its risk factors and survival outcomes. Results: A total of 406 patients with DLBCL were identified, and 17 (4.2%) of DLBCL patients had CNS involvement i.e. 9 (2.2 %) at diagnosis and 8 (~2%) at relapse. The patients were younger, had advanced stage, high CNS-IPI, and had extra nodal involvement. Seven out of the 17 patients who survived received chemotherapy and a prophylactic methotrexate. Considering the CNS-IPI, of the 146 patients with high CNS-IPI at presentation, 18 received the prophylactic HDMTX and 3 (16.7%) of them had CNS relapse. Two (1.6%) out of 128 who did not receive the prophylactic HDMTX had CNS relapse. On the other hand, of the 223 patients with intermediate CNS-IPI, 25 received the prophylactic HDMTX and 2 (8%) of them had CNS relapse and in 198 patients who did not receive the prophylactic HDMTX, 2 (1.01%) had CNS relapse. The 5-year progression-free survival and overall survival rates for the entire cohort were 73% and 84%, respectively. The median OS for those who had CNS involvement was 17 months and the 2-year OS was 40%. Conclusion: CNS involvement in DLBCL has a poor prognosis, thus, aggressive CNS-directed therapy should be considered, especially in young patients.https://journal.waocp.org/article_90498_6cb3218d0030914d5d9885876f84de7d.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Reflections from Women with an Interval Breast Cancer Diagnosis: A Qualitative Analysis of Open Disclosure in the BreastScreen Western Australia Program6336399050010.31557/APJCP.2023.24.2.633ENLilyClaringboldNational School of Medicine, The University of Notre Dame Australia, Sydney, NSW, Australia.Meagan EBrennanNational School of Medicine, The University of Notre Dame Australia, Sydney, NSW, Australia.0000-0002-3281-7643HelenLundBreast Screen Western Australia, Women and Newborn Health Service, Perth, WA, Australia.SoniaEl-ZaemeyBreast Screen Western Australia, Women and Newborn Health Service, Perth, WA, Australia.NehmatHoussamiSydney School of Public Health, Faculty of Medicine and Health, The University of Sydney, NSW and The Daffodil Centre, the University of Sydney, a joint venture with Cancer Council NSW, Australia.ElizabethWylieBreast Screen Western Australia, Women and Newborn Health Service, Perth, WA, Australia.School of Medicine, University of Western Australia, Perth, Western, Australia.Journal Article20220924Background: ‘Interval breast cancer’ describes a malignancy that is diagnosed after a negative screening mammogram. Open disclosure is a process of addressing a negative health outcome that includes an apology and an opportunity for the client to discuss concerns. BreastScreen Western Australia has implemented a policy of open disclosure. The purpose of this study was to gain an understanding of clients’ experience with interval cancer and their attitude towards the screening programme by conducting a thematic analysis of written responses from women participating in the open disclosure process. Methods: Women experiencing an interval cancer diagnosis between 2011 and 2020 were sent a questionnaire by mail. It included two broad questions with free-text responses. A qualitative analysis of the responses was conducted using an inductive approach. Responses were de-identified and data were thematically analysed and presented using verbatim quotations. Results: Five themes emerged in response to “what could we have done better?”: ‘nothing,’ ‘broaden scope,’ ‘service delivery,’ ‘breast density education’ and ‘more education’ generally. Six themes emerged in response to “what did we do well?”: ‘staffing,’ ‘overall satisfaction,’ ‘reminders,’ ‘follow-up after interval cancer,’ ‘efficiency’ and ‘information and education provision.’ An additional theme of ‘storytelling’ emerged from both questions: an opportunity for the woman to share her experience of cancer. Conclusion: Most women expressed positive attitudes towards the service and appreciated giving feedback in the open disclosure process. Several themes supporting the role of BreastScreen in education were identified, including providing information about breast density, breast health, and limitations of screening. https://journal.waocp.org/article_90500_34074ba2ee19575fc84e339359c00f23.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Factors Affecting Quality of Life of Cancer Patients in North India6416489050110.31557/APJCP.2023.24.2.641ENKapilKumarDepartment of Community Medicine, Maharishi Markandeshwar, Institute of Medical Sciences and Research, Mullana, Ambala,
Haryana, India.0000-0003-3541-8784AnshuMittalMaharishi Markandeshwar Deemed to be University, Mullana (Ambala), India.DeepmalaKambojDepartment of Mathematics, Mukand Lal National College, Yamunanagar, Haryana, India.0000000295202080Gauri ShankarGoelDepartment of Community Medicine, Maharishi Markandeshwar, Institute of Medical Sciences and Research, Mullana, Ambala, Haryana, India.AnujMittalDepartment of Community Medicine, Atal Bihari Vajpayee Government Medical College, Faridabad, Haryana, India.Sumesh KumarGargDepartment of General Surgury, Adesh Medical College, Shahbad, Haryana, India.Journal Article20220924Introduction: Cancer is the second leading reason of death in many countries, and its burden and prognosis are highly dependent on disease stage at diagnosis. Lack of perception of the population about cancer and its related risk factors usually sequel in defer/delayed presentation and increased treatment morbidity and decrease survival chances. Quality of life (QOL) is defined as Individual view of life, merits, intentions, standards and interests in the framework of culture. Aims and objectives: To study the socio-demographic profile and factors affecting quality of life of cancer patients. Material and Method: Using a cross-sectional study, between 1 January 2021 to 31 December 2021, 192 cancer confirmed patients at cancer registry centre at District civil hospital, Ambala cantt. were interviewed using a pre tested semi-structured questionnaire along with EORTC QLQ-C30 (version 3) questionnaire scale for QOL. The data was entered into an excel sheet and was analysed using IBM SPSS version 28. Results: Out of 192 patients, 95 were male and 97 were female. Results shows that female (60.05±19.167) had better mean score of QL2 than male (58.68±18.906) with P value=0.619. Overweight (BMI25.0-29.9) had better mean QL2 score (65.50±18.147) than obese (BMI>30.0) mean QL2 score (61.67±14.803), normal weight (BMI 18.0-24.9) mean QL2 score (59.57±18.575) and underweight (BMI<18.0) mean QL2 score (49.46±19.595) with P value of 0.004. Conclusion: Present study found that QOL had significant association with BMI, type of cancer, history of smoking, lost income after cancer diagnosis and stage of cancer at the time of diagnosis. While gender, occupation, age group, religion, residence, marital status, type of family, income, tobacco alcohol and physical activity showed no significance association with QOL.https://journal.waocp.org/article_90501_0f871538b4d19c4b411484802fd8b5ec.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Neural Differentiation of Wisdom Tooth Follicle Stem Cells on a Nano-Hydrogel Scaffold Containing Salvia Chloroleucat to Treat Nerve injury in the Cancer of Nervous System6496589052010.31557/APJCP.2023.24.2.649ENNaghmehBahramiDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran,
Iran.Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.0000-0002-3575-4094ZaynabManafiCraniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran.FarnoushMohammadiOral and Maxillofacial Surgery
Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.Seyedeh ZahraFotook KiaeiDepartment of Pulmonary and Critical
Care, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.AbdollahFarhadi NasabBehavioral Sciences Research Center, Taleghani
Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Seyed HosseinHosseini LarganiDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran,
Iran.MerajNaghdiIslamic Azad University of Medical Sciences, Tehran,
Iran.AbdolrezaMohamadniaChronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD),
Shahid Beheshti University of Medical Sciences, Tehran, Iran.School of Advanced Technologies in Medicine, Department of
Biotechnology, Shahid Bheshti University of Medical Sciences, Tehran, Iran.Journal Article20220926The human third molar’s follicle is one of the sources of stem cells with high differentiation capacities which can be used in nervous system cancer treatment particularly in nerve damge. The purpose of this research was to identify the effects of the aqueous extract of Salvia chloroleuca on the differentiation of the human dental follicle-derived mesenchymal stem cells to neural cells for treti. In this experimental study, the method of culture of digested tissue fragments was used to isolate stem cells from three samples of the extracted wisdom teeth follicles. The nano-hyaluronic acid scaffold has been synthesized by the sol-gel method as a porous composite and the S. chloroleuca extract has been loaded into it. The scaffold was analyzed in terms of mechanical properties, drug release and toxicity. Afterwards, the cells were seeded onto the scaffold using the immersion method. After 21 days, cell differentiation was investigated by morphological confirmation methods and confirming the expression of β-tubulin and MAP2 genes at mRNA and protein levels. Morphological assessment revealed neural differentiation in the cells of the groups of nano-hyaluronic acid scaffold with S. chloroleuca extract and nano-hyaluronic acid scaffold with S. chloroleuca extract + 10% retinoic acid. Furthermore, the expression of MAP2 and β-tubulin in these groups was confirmed by RT-PCR, real time PCR and western blot assays. The results of this research showed that the follicle of the third molar contains stem cells with a high capacity for differentiation. Moreover, the extract of S. chloroleuca, could lead to induction of neural differentiation in stem cells.https://journal.waocp.org/article_90520_3177ff2c0fc561dd49812ce11508cb65.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Assessment of the Association of Chlamydia e pneumoniae Infection with Lung Cancer in a Moroccan Patients’ Cohort6596659050210.31557/APJCP.2023.24.2.659ENMeryemChebakFundamental Sciences Department, Faculty of dental medicine, Mohammed V University in Rabat, Morocco.Faculty of Sciences, Mohammed V University in Rabat, Morocco.MaryameAzzouziFaculty of Sciences Aïn Chock, Hassan II University de Casablanca. Morocco.Research Department, Chlamydia e & Mycoplasmas Laboratory, Institut Pasteur du Maroc, Casablanca 20360, Morocco.HoudaChaibiFundamental Sciences Department, Faculty of dental medicine, Mohammed V University in Rabat, Morocco.MeryemFakhkhariFundamental Sciences Department, Faculty of dental medicine, Mohammed V University in Rabat, Morocco.IchrakBenamriResearch Department, Chlamydia e & Mycoplasmas Laboratory, Institut Pasteur du Maroc, Casablanca 20360, Morocco.Systems & Data Engineering Team, National School of Applied Sciences of Tangier, Morocco.MalikaMguilFundamental Sciences Department, Faculty of dental medicine, Mohammed V University in Rabat, Morocco.KhadijaHajjoutNational Blood Transfusion Center, Rabat, Morocco.AdilZegmoutDepartment of Pulmonology, Mohammed V Military University Hospital, Rabat, Morocco.NabilTiresseDepartment of Pulmonology, Mohammed V Military University Hospital, Rabat, Morocco.Ismail AbderrahmaneRhorfiDepartment of Pulmonology, Mohammed V Military University Hospital, Rabat, Morocco.HichamSouhiDepartment of Pulmonology, Mohammed V Military University Hospital, Rabat, Morocco.AhmedAbidDepartment of Pulmonology, Mohammed V Military University Hospital, Rabat, Morocco.RachidaZahraouiFaculty of Medicine and Pharmacy, Mohamed V University in Rabat, and Moulay Youssef Hospital, Rabat, Morocco.Jamal EddineBourkadiFaculty of Medicine and Pharmacy, Mohamed V University in Rabat, and Moulay Youssef Hospital, Rabat, Morocco.HichamOumzilMicrobiology Pedagogy and research unit of school of Medicine and Pharmacy, Mohamed V University in Rabat, Virology department National Institute of Hygiene, Rabat, Morocco, and university Mohammed V, Rabat, Morocco.FouziaRadouaniResearch Department, Chlamydia e & Mycoplasmas Laboratory, Institut Pasteur du Maroc, Casablanca 20360, Morocco.KhalidSadkiFundamental Sciences Department, Faculty of dental medicine, Mohammed V University in Rabat, Morocco.Journal Article20220930Background: Chlamydia pneumoniae (C. pneumoniae) is a respiratory pathogen associated with chronic inflammatory and its detection in human lung cancer suggests its involvement in cancerogenesis. Our study aimed to evaluate the association between C. pneumoniae infection and Lung Cancer disease in Moroccans patients and control cohorts, through a molecular investigation. Methods: The study comprised 42 lung cancer patients and 43 healthy controls. All participants provided demographics, Clinical, and Toxic behaviors datas, and a peripheral blood sample for testing, a Nested Polymerase Chain Reaction (PCR) was performed for C. pneumoniae Deoxyribonucleic acid (DNA) detection. Statistical analysis was performed using IBM®SPSS®software. Results: Positive Nested PCR results for cases and controls were respectively 33.3% and 4.7%, there by significant difference between cases and controls infection was identified (p <0.05). Data analysis also showed that tobacco could act synergically with C. pneumoniae infection as a risk factor of lung cancer. In fact a significant difference between patients and controls was shown for tobacco and alcohol use (p < 0.05). Conclusion: C. pneumoniae infection is potentially associated with primary Lung cancer in the Moroccan population and has combined effects with Tabaco consumption.https://journal.waocp.org/article_90502_45c3d998c6469528a159ae942608fad2.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Role of Cryptosporidium spp in Development of Colorectal Cancer6676749050410.31557/APJCP.2023.24.2.667ENNaglaa FathiAbd El-LatifDepartment of Parasitology, Medical Research Institute, Alexandria University, Egypt.Noha SaidKandilDepartment of Chemical Pathology, Medical Research Institute, Alexandria University, Egypt.MohamedShamseyaDepartment of Internal Medicine, Medical Research Institute, Alexandria University, Egypt.Yasmine NagyElwanyDepartment of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt.Heba SaidIbrahimDepartment of Parasitology, Medical Research Institute, Alexandria University, Egypt.Journal Article20221006Background: Colorectal cancer is one of the most common malignancies in humans. About 20% of the cancer incidence was attributed to infectious agents highlighting the association between infectious agents and the development of cancers. It has been suspected that Cryptosporidium spp. infection may be correlated with colon adenocarcinoma. Aim: investigate the percentage of cryptosporidiosis among colorectal cancer patients. Subjects: 100 patients were recruited from Medical Research Institute, Alexandria University. Methods: Fresh stool specimens were collected, homogenized and examined using direct wet mount and by permanent staining of faecal smears using Modified ZN staining. Molecular detection by PCR amplification of Cryptosporidium COWP gene. Results: Significantly higher proportion of colorectal cancer patients (32.5%, 42.5%) tested positive by MZN and ELISA respectively compared to only 3.3% and 5% of positive MZN and ELISA among control group. Also, positive PCR was detected among higher proportion of colorectal cancer patients (47.5%) and only 5% of control group. Odds of colorectal cancer is 19 times among positive cases of Cryptosporidium by PCR than those without proven infection by PCR (OR 19.12; 95% CI 4.82-75.99). Comparison of the assessment of Cryptosporidium infection made by two techniques produces a kappa value of 0.770, and .759 respectively between NZN, ELISA and PCR as a gold standard, suggesting a good agreement between the two techniques and PCR. This value of kappa is significantly different from zero, K.770, p<0.001 for MZN and K.759, p<.001 for ELISA. Specificity of MZN (100%) is higher than that of ELISA (96.2%) and both reported higher specificity than sensitivity denoting that both tests are good positive to rule in the presence of infection at 40% prevalence. Conclusion: Cryptosporidium infection is significantly higher among cancer colon patients reinforcing that it might be considered as a likely risk factor for the development cancer colon.https://journal.waocp.org/article_90504_456efd96c8be29395c53e2fb344b9907.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Retailer’s Density and Single Stick Cigarette’s Accessibility among School-Age Children in Indonesia6756829050510.31557/APJCP.2023.24.2.675ENRisky KusumaHartonoDepartment of Public Health, Universitas Indonesia Maju, Jakarta, Indonesia.0000-0003-3703-4215Rizki FajarMeirawanDepartment of Public Health, Universitas Indonesia Maju, Jakarta, Indonesia.RennyNurhasanaUrban Studies Program, School of Strategic
Global Studies, Universitas Indonesia, Indonesia.0000-0003-3154-8223TeguhDartantoFaculty of Economics and Business, University of Indonesia, Indonesia.0000-0002-1737-3650AryanaSatryaFaculty of Economics and Business, University of Indonesia, Indonesia.Center for Social Security Studies, School of Strategic and Global Studies Universitas Indonesia.0000-0003-2745-4849Journal Article20221008Objective: The density of single-stick cigarette sales is related to the increase in tobacco epidemic-related diseases. This study aims to provide evidence of retailers’ density and radius around the school location, accessibility of single-stick cigarette selling among school-age children, and retailers’ response regarding the restriction policy options in urban areas in Indonesia. Methods: It is a cross-sectional study. The retailers’ spatial density and the radius around schools in Daerah Khusus Ibukota (DKI) Jakarta Province were investigated using Google Maps and Google Street View (GSV). The coordinates of retailers and schools were geo-coded to Kernel Density Map. The accessibility of single-stick cigarettes among children and restriction policy options for cigarette selling were derived from random sampling using surveys of 64 retailers based on Google Data results. Result: Virtually walking using google maps and GSV found 8,371 retailers in DKI Jakarta. There were ± 15 cigarette retailers every 1 km2, and an average of ± one cigarette retailer in every 1,000 residents. There were 456 (21.67%) retailers with a radius ≤ 100 meters around elementary schools, even an increase around junior high school locations of 167 (26.05%) retailers. The accessibility of cigarettes among children is easy because the price is relatively low, at Rp1,500/ $0.11 per stick. In addition, 58.1% of retailers allowed customers to buy on debt. Eleven percent of cigarette retailers intended to reduce the sale of cigarettes if the prohibition of single-stick cigarette sales were applied. Conclusion: Cigarette retailers were very dense and single-stick cigarettes were still accessible to children in Indonesia. The implementation of the prohibition on single-stick cigarette sales should be added for future tobacco control in developing countries such as Indonesia.https://journal.waocp.org/article_90505_fe8a1a4243495965a102bbedc541d0d9.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Chalcone-3 Inhibits the Proliferation of Human Breast Cancer MDA-MB-231 Cell Line6836919050610.31557/APJCP.2023.24.2.683ENNovritaPadaulengDoctoral Program, Faculty of Medicine, Public Health and Nursing (FKKMK), Universitas Gadjah Mada, Yogyakarta, Indonesia.Department of Biomedicine, Faculty of Medicine, Universitas Mataram, Indonesia.0000000245647233MustofaMustofaDepartment of Pharmacology and Therapy, Faculty of Medicine, Public Health and Nursing (FKKMK), Universitas Gadjah Mada, Yogyakarta, Indonesia.0000000192519851Tutik DwiWahyuningsihDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia.0000000157412848DewajaniPurnomosariDepartment of Histology and Cell Biology, Faculty of Medicine, Public Health and Nursing (FKKMK), Universitas Gadjah Mada, Yogyakarta, Indonesia.0000-0002-3972-8387Journal Article20221014Objective: Chalcone-3 has been shown to be cytotoxic and selective against luminal subtype breast cancer cell lines, which are suspected to occur through the mechanism of epidermal growth factor receptors (EGFR) inhibition. However, the cytotoxic effect has never been tested on cell strains from patients with triple negative breast cancer (TNBC), where EGFR expression is known to increase. This study aimed to identify the role of chalcone-3 in one of the downstream targets of EGFR as an antiproliferative agent. Methods: Chalcone-3 was examined for its effect on proliferation in human breast cancer MDA-MB-231 cell lines. The percentage of proliferation inhibition was analyzed using methyl-thiazol tetrazolium assay. Flow cytometry was used to analyze the population of cell cycle distribution and the expression of cyclin-D1 and pEGFR. Results: Chalcone-3 inhibited the proliferation of MDA-MB-231 cells in a dose and time-dependent manner with an IC50 value of 17.98±6.36 µg/mL by inducing cell cycle arrest at the G2/M phase. Flow cytometry assays showed that chalcone-3 significantly reduced the expression of pEGFR and cyclin-D1, contributing to cell cycle arrest. Conclusion: Chalcone-3 might have potential as an anti-proliferative drug to treat TNBC. https://journal.waocp.org/article_90506_9fbc48115309fb00957e643fc894db15.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Endometrial Thickness Measurement as Predictor of Endometrial Hyperplasia and Cancer in Perimenopausal Uterine Bleeding: Cross-Sectional Study6936999050710.31557/APJCP.2023.24.2.693ENPurinThoprasertDepartment of Obstetrics and Gynecology, Bhumibol Adulyadej Hospital, Bangkok, Thailand.PaweenaPhaliwongDepartment of Obstetrics and Gynecology, Bhumibol Adulyadej Hospital, Bangkok, Thailand.BuppaSmanchatDepartment of Obstetrics and Gynecology, Bhumibol Adulyadej Hospital, Bangkok, Thailand.SinartPrommasDepartment of Obstetrics and Gynecology, Bhumibol Adulyadej Hospital, Bangkok, Thailand.KornkarnBhamarapravatanaDepartment of Preclinical
Science, Faculty of Medicine, Thammasat University Hospital, Bangkok, Thailand.KomsunSuwannarurkGynecologic Oncology Unit, Department
of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University Hospital, Bangkok, Thailand.0000 0003 3019 9877Journal Article20221017Background: Endometrial thickness (ET) measurement was an alternative method for predicting abnormal endometrial pathology in postmenopausal bleeding. Cut-off value of ET measurement could not be use in perimenopausal bleeding. Objective: Aim of this study was to investigate appropriate ET cut-off value for perimenopause women with abnormal uterine bleeding (PEMB) and abnormal endometrial histopathology. Material and methods: This was a cross-sectional study. PEMB at Bhumibol Adulyadej Hospital between July 2018 and June 2022 were recruited. Subjects who met inclusion criteria underwent ET measurement and endometrial biopsy via endometrial aspirator. Participants who had histopathology report of endometrial hyperplasia and more were classified as the study group. The Control group were subjects with no endometrial hyperplasia or cancer. Demographic and clinical character data were included. Correlation of ET and endometrial histopathology were calculated for statistical significance. Results: A total of 304 cases were included. After exclusion, 254 subjects were recruited for this study. There were 22 and 232 cases in the study and control groups, respectively. The mean age and body mass index (BMI) of participants were 44.7 years old and 27.5 kg/m2, respectively. Prevalence of endometrial hyperplasia and cancer in perimenopausal uterine bleeding were 7.5 (19/254) and 1.2 (3/254) percent, respectively. Endometrial thickness equal to and more than 8 mm was associated with abnormal endometrial histopathology with statistical significance. Age, BMI, nulliparity, anovulatory bleeding history, hypothyroidism, diabetes mellitus and anovulation state of both groups were comparable. Conclusions: Endometrial thickness equal or more than 8 mm were significantly associated with endometrial hyperplasia or more among perimenopausal women with abnormal uterine bleeding.https://journal.waocp.org/article_90507_99964c0ea9e9a04a96948f62d253caae.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Neutrophil-to-Lymphocyte Ratio (NLR) for Preoperative Differentiation between Uterine Leiomyosarcoma (LMS) and Uterine Leiomyoma: A Case-Controlled Study7017079050810.31557/APJCP.2023.24.2.701ENPhitsineeSrisuthaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.SomsookSantibenchakulDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.Department
of Obstetrics and Gynecology, King Chulalongkorn Memorial Hospital, Bangkok, Thailand.ChaiAriyasriwatanaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.0000-0002-7049-1293ShinaOranratanaphanDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.0000000337387061Journal Article20221022Background: Differentiating uterine LMS from uterine leiomyoma is difficult. Therefore, this study aimed to compare preoperative NLR of uterine leiomyosarcoma (LMS) with leiomyoma and secondary objective aimed to identify the clinical characteristics to distinguish between uterine LMS and uterine leiomyoma including the appropriate NLR cut off value to differentiate LMS from leiomyoma. Methods: This was a matched case-controlled study with 1:4 ratio. We collected data of patients with uterine LMS and leiomyoma from 2011 to 2020 at King Chulalongkorn Memorial Hospital. Patients with uterine LMS (case group) and leiomyoma (control group) were matched in terms of year of the surgery and size of the uterine mass. Statistical analysis was conducted using SPSS version 22.0 and STATA version 17. Conditional logistic regression analysis with a p-value of <0.05 was used. Results: Twenty-seven patients who were diagnosed with uterine LMS met the inclusion criteria; 13 patients who had incomplete data and one patient who was had concurrent breast cancer were excluded. Thirteen patients were included in the final analysis. From 2,587 patients in control group; 52 patients were matched. The baseline characteristics in both groups were comparable except for menopausal status Women with uterine LMS had a higher NLR than those with leiomyoma (mean, 4.56 ± 2.5 and 2.4 ± 1.15 in the case and control groups, respectively). Conditional logistic regression determined that the NLR cut-off value of 2.8 was a statistically significant factor for determining uterine LMS (OR = 3.24; 95% CI 1.01–10.43). No significant difference was found in the other factors. Conclusions: Patients who were diagnosed with uterine LMS had a significantly higher NLR than those diagnosed with leiomyoma. The NLR is a simple and effective method for predicting the presence of a uterine LMS in patients who are pre-operatively diagnosed with a uterine mass.https://journal.waocp.org/article_90508_e8ece4e5967a951050003007327fe672.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Mutational Damages in Malignant Lung Tumors7097169050910.31557/APJCP.2023.24.2.709ENSauleYermekovaChair of Molecular Biology with Courses in Chemistry and Biochemistry, Kazakhstan-Russian Medical University, Almaty,
Republic of Kazakhstan.MadinaOrazgaliyevaMolecular Genetic Research Center, Kazakh Institute of Oncology and Radiology, Almaty, Republic of
Kazakhstan.TatyanaGoncharovaDepartment of Scientific Management and Grant Research, Kazakh Institute of Oncology and Radiology, Almaty,
Republic of Kazakhstan.FaridaRakhimbekovaDepartment of Chemical and Biochemical Engineering, Institute of Chemical and Biological Sciences,
Satbayev University, Almaty, Republic of Kazakhstan.ZaureDushimovaDepartment of Research and Strategic Development, Kazakh Institute of
Oncology and Radiology, Almaty, Republic of Kazakhstan.TatianaVasilievaDepartment of Research and Strategic Development, Kazakh Institute of
Oncology and Radiology, Almaty, Republic of Kazakhstan.Journal Article20221028Background: Today, genomic changes are an important cause of the occurrence, growth and progression of cancer. Technological advances in cancer genomic analysis platforms have made it possible to identify genomic alterations that may influence response to lung cancer treatment. Methods: The study examined tumor growth-inhibiting oncogenes and genes responsible for cell growth and division to identify mutations characteristic of malignant lung tumors. The mutations were studied in 400 postoperative samples after amplifying p53 and HRAS fragments and p53, p21Waf1, MDM2 mRNA. p53 or p21Waf1 were expressed in 50% of squamous cell carcinomas and adenocarcinomas of the lung. Results: The study examined tumor growth-inhibiting oncogenes and genes responsible for cell growth and division to identify mutations characteristic of malignant lung tumors. The mutations were studied in 400 postoperative samples after amplifying p53 and HRAS fragments and p53, p21Waf1, MDM2 mRNA. p53 or p21Waf1 were expressed in 50% of squamous cell carcinomas and adenocarcinomas of the lung. HRAS mutations were present in most squamous cell carcinomas and adenocarcinomas of the lung. EcoR1- and Pst1- restriction enzymes destroyed the RT-PCR product of the p53 and p21Waf1 mRNA and increased the level of detected mutations in lung adenocarcinoma to 75% and 50 %, respectively. EGFR mutations were more frequent in lung adenocarcinoma than in lung squamous cell carcinoma. Mutations in EGFR exons 19 and 21 found in 65 of 263 lung tumor samples indicated the tumor sensitivity to EGFR tyrosine kinase inhibitors. EGFR deletions in exon 19 occurred mainly in adenocarcinoma, L858R mutations in EGFR exon 21 were quite common in lung adenocarcinoma. Conclusion: The mutations detected in most squamous cell carcinomas and adenocarcinomas of the lung could be used to diagnose and predict the disease severity and targeted therapy efficacy.https://journal.waocp.org/article_90509_592c9e770c498c2d43bac11d1a80d71d.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201The Association Between the Serum Level of IGF-1 and IGFBP-3 and the Risk of Breast Cancer among Women in the Gaza Strip7177239051010.31557/APJCP.2023.24.2.717ENHeba MohammedArafatDepartment of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian
16150, Kelantan, Malaysia.0000-0003-2710-5673JuliaOmarDepartment of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian
16150, Kelantan, Malaysia.NoorazliyanaShafiiDepartment of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian
16150, Kelantan, Malaysia.Ihab AliNaserDepartment of Clinical Nutrition, Faculty of Applied Medical Sciences, Al- Azhar University- Gaza,
Gaza City, Palestine.0000-0002-9479-7748Nahed AliAl LahamDepartment of Laboratory Medicine, Al Azhar University-Gaza, Gaza City, Palestine.RosedianiMuhamadDepartment of Family
Medicine, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.Tengku Ahmad DamitriAl-AstaniDepartment of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian
16150, Kelantan, Malaysia.0000-0003-3265-1679Ohood MohammedShamallakhDepartment of Medical Laboratory Sciences, Faculty of Health Sciences, Islamic University of Gaza, Gaza City, Palestine.Kholoud MohammedShamallakhDepartment of Medical Laboratory Sciences, Faculty of Health Sciences, Islamic University of Gaza, Gaza City, Palestine.Journal Article20221103Objective: The purpose of this research was to look at the relationship between insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) levels and the risk for breast cancer (BC) among women in the Gaza Strip. Methods: This case-control study, which included 334 participants (112 women with BC as cases, and 222 women without BC as control), was conducted between January 2021 and August 2022. Research data for the cases were gathered at the Oncology Departments of the Gaza Strip’s two hospitals, “Al- Shifa Hospital and Turkish Palestinian Friendship Hospital,” as well as from the electronic records of the Screening Mammogram Unit at Al- Remal Clinic for controls. The information about the participants was gathered using a standardized questionnaire. The key variables related to BC were identified using multivariate logistic regression. Results: According to multivariate logistic regression, participants’ age was associated with an increase in the risk of BC (OR= 1.03; 95%CI, 1.007-1.060). There was an association between serum concentrations of fasting blood glucose (FBG) (OR= 1.027; 95% CI, 1.013–1.042), IGF-1 (OR= 1.010; 95% CI, 1.006–1.015), and the risk of BC, while there was no link between IGFBP-3 and the risk of BC. In an analysis of the risk according to menopausal status, premenopausal women were associated with an approximate 0.5 time decrease in risk of BC compared to women in post-menopause (OR= 0.428, 95% CI, 0.258, 0.710). Dairy product was also related to a decreased risk of BC. Conclusion: The results suggest that age, lower physical activity, increased levels of FBG, and IGF- 1 increase the BC risk among females in the Gaza Strip. Meanwhile, premenopausal women and dairy products are linked to a reduction in the risk of BC. Furthermore, no link was found between IGFBP-3 and BC risk. Improving early BC detection rates in the Gaza Strip necessitates preventative interventions and screening for BC in the public and healthcare sectors.https://journal.waocp.org/article_90510_ba34b2ad7fb1422fcccea458bc3dccee.pdfWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736824220230201Capacity Building of a Self-Reliant Model Community for Cholangiocarcinoma Prevention by Producing Fruit and Vegetable Juice Products in a High-Risk Area of Thailand7257319051110.31557/APJCP.2023.24.2.725ENChaiyakritYokphonchanachaiDepartment of Occupational Health and Safety, Faculty of Public Health, Ubon Ratchathani Rajabhat University, Ubon
Ratchathani, Thailand.0000-0002-0941-6411NopparatSongsermDepartment of Health Sciences, Faculty of Public Health, Ubon Ratchathani Rajabhat University, Ubon
Ratchathani, Thailand.0000-0003-3741-367XSumapornThongprungDepartment of Community Health, Faculty of Public Health, Ubon Ratchathani Rajabhat University,
Ubon Ratchathani, Thailand.0000-0002-1534-9040ComsunThongchaiDepartment of Occupational Health and Safety, Faculty of Public Health, Ubon Ratchathani Rajabhat University, Ubon
Ratchathani, Thailand.0000-0002-5717-1834YanithaPaengprakhonDepartment of Occupational Health and Safety, Faculty of Public Health, Ubon Ratchathani Rajabhat University, Ubon
Ratchathani, Thailand.0000-0002-8458-153XJarupornDuangsriDepartment of Occupational Health and Safety, Faculty of Public Health, Ubon Ratchathani Rajabhat University, Ubon
Ratchathani, Thailand.0000-0002-5500-7213OnwanyaSriwaromNong Lak Sub-District Health Promoting Hospital, Muang Sam Sip District, Ubon Ratchathani,
Thailand.0000-0001-6632-8285Journal Article20221120Ojective: This participatory action research aims to develop healthy fruit and vegetable juice products (FVPs) to enhance people’s income and quality of life (QOL) in a community with the highest prevalence of cholangiocarcinoma (CCA) in Thailand. Methods: We randomly sampled study areas from 25 homes in a Thai community to solve poverty and improve QOL. Descriptive and inferential statistics were employed for data analysis. Moreover, qualitative data were analyzed by content analysis. Results: The community management system relies on local resources under religious inspiration. Academics also keep developing FVPs from local raw materials planted by organic farming under “Nong Lak Rak Sukapab.” The product’s nutritional value was measured by In-House and Nutrition Labeling Methods from National Food Institute Laboratory (IEC/ISO17025). According to the Bureau of Nutrition, a 230 ml bottle of each wheatgrass and corn juice contains nutrients beneficial to health and necessary for the body. In addition, the community has participated in production planning, marketing, distribution, and household accounting. As a result, average monthly household income and QOL increased with statistical significance after the project implementation. Conclusions: These findings demonstrated that improving communities’ capacity to be health leaders by producing FVPs to prevent CCA in high-risk areas is critical to early disease prevention and community health care. These issues can also be applied to public policy implications for other regions and diseases in order to emphasize community health care and long-term community development.https://journal.waocp.org/article_90511_4480bdafd02ee5c4e03b137bb048db3c.pdf