West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Revival of Cancer Registration in Karachi, Pakistan118941210.31557/APJCP.2021.22.1.1ENMuhammad AsifQureshiDow International Medical College, Dow University of Health
Sciences, Karachi, Pakistan.0000-0002-8370-1069Journal Article20201216West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Prognostic Value of Two Polymorphisms, rs1045642 and rs1128503, in ABCB1 Following Taxane-based Chemotherapy: A Meta-Analysis3108941310.31557/APJCP.2021.22.1.3ENQuanyaoChenWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.WanlongLinWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.JianhuiYangWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.MinLinWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.XiuxianLinWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.YiyinWengWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.YaoChenWomen and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China.Journal Article20200924Objective: Genetic polymorphisms can influence the chemotherapeutic response; however, previous studies have produced conflicting results, and have failed to identify the most relevant polymorphisms for predicting the response to treatment in patients with cancer. The present meta-analysis was conducted to determine the correlation between two polymorphisms (rs1045642 and rs1128503) in ATP-binding cassette transporter B subfamily member 1 (ABCB1), which is associated with multidrug resistance, and the survival of patients treated with taxane-containing chemotherapy. Methods: Several databases, including PubMed and Embase, were used to retrieve articles evaluating the association between the ABCB1 rs1045642 and rs1128503 polymorphisms and survival, published prior to August 2019. The meta-analysis was conducted using R software to determine the pooled hazard ratio (HR) and 95% confidence intervals (95% CIs). Results: Fifteen studies involving 3320 patients were included in the meta-analysis. The effect of the rs1128503 polymorphism on progression-free survival remained significant in the heterozygote (HR 0.81; 95% CI: 0.67–0.98) and homozygote (HR 0.71; 95% CI: 0.58–0.88) models. The TT genotype rs1128503 was associated with better overall survival (HR 0.72; 95% CI: 0.53–0.97). Conclusion: Carriers of the rs1128503 T allele of ABCB1 showed a survival benefit after taxane-containing chemotherapy.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101HER2Ile655Val Single Nucleotide Polymorphism Associated with Early-Onset Breast Cancer Susceptibility: A Systematic Review and Meta-Analysis11188941410.31557/APJCP.2021.22.1.11ENTungNguyen ThanhInstitute of Biomedicine, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue, Vietnam.Faculty
of Basic Science, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue, Vietnam.0000-0002-5215-383XBao SongNguyen TranDepartment of
Histology, Embryology, Pathology and Forensic, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street,
Hue, Vietnam.0000-0002-7740-5984Ai PhuongHoang ThiFaculty
of Basic Science, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue, Vietnam.0000-0002-7902-6996ThangTran BinhFaculty of Public Health, Hue University of Medicince and Pharmacy, Hue university, 6 Ngo Quyen Street, Hue,
Vietnam.0000-0002-8179-4927ThongBa NguyenDepartment of Medical Bioscience, Soonchunhyang University Hospital Bucheon, Bucheon 14584, Republic of Korea.0000-0001-9647-534XTamLe MinhDepartment of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue,
Vietnam.0000-0002-8296-887XQuoc HuyNguyen VuDepartment of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street, Hue,
Vietnam.0000-0002-4744-7059ThuanDang CongDepartment of
Histology, Embryology, Pathology and Forensic, Hue University of Medicine and Pharmacy, Hue University, 6 Ngo Quyen Street,
Hue, Vietnam.0000-0002-2657-6791Journal Article20201008ackground: Human epidermal growth factor receptor 2 (HER2) plays an important role in the development and progression of breast cancer. To understand the precise association, this meta-analysis was conducted to estimate the association between HER2Ile655Val single nucleotide polymorphism (SNP) and susceptibility to early-onset breast cancer. Methods: A comprehensive database retrieval from PubMed, Embase, Web of Science and Google Scholar was pooled to investigate links between the HER2Ile655Val SNP and risk of breast cancer. Adjusted odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to appraise the association under the additive model (Ile vs. Val), dominant model (Val/Val + Ile/Val vs. Ile/Ile), and recessive model (Val/Val vs. Ile/Val + Ile/Ile). Results: Seventeen relevant studies with 11,749 cases and 8,105 controls were finally included. We found that HER2Ile655Val SNP is associated with an increased risk of breast cancer in an additive and dominant model. In the subgroup analysis with age stratification, a significant association between the HER2 codon 655 SNP and the risk of breast cancer was found in young women in an additive, dominant, and recessive model; conversely, no significant associations were indicated in older women. In the breast cancer subgroup, HER2Ile655Val SNP was significantly associated with younger age women with breast cancer in the dominant model. In contrast, no association between the HER2 codon 655 SNP and age was found in control populations. Conclusion: Our findings suggest that the Val allele in HER2 codon 655 SNP is strongly associated with breast cancer susceptibility in the young female population and is also significantly associated with younger age in women with breast cancer. HER2Ile655Val SNP might be a susceptibility factor that favours early-onset breast cancer.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Review on the Oncology Practice in the Midst of COVID-19 Crisis: The Challenges and Solutions19248941510.31557/APJCP.2021.22.1.19ENPedramFadaviDepartment of Radiation Oncology, Iran University of Medical Sciences, Tehran, Iran.0000-0001-7491-2561MohammadHoushyariDepartment of Clinical Oncology,
Shohada-e Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Amir ShahramYousefi KashiDepartment of Clinical Oncology,
Shohada-e Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Cancer Research Center, Faculty
of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Alireza MosaviJarrahiCancer Research Center, Faculty
of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0001-8043-9221FarnazRoshanmehrFaculty of Science and Engineering, Waseda
University, Tokyo, Japan.Kagawa Nutrition University, Saitama, Japan.Mohammad AliBroomandDepartment of Clinical Oncology, Shahid Sadoughi
University of Medical Sciences, Yazd, Iran.SalehSandoughdaranShohada Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran,
Iran.FarzadTaghizadeh-HesaryDepartment of Clinical Oncology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-6195-2203Journal Article20201124As of late 2019, the outbreak of novel coronavirus disease (COVID-19) –that started in China– has rapidly afflicted all over the world. The COVID-19 pandemic has challenged health-care facilities to provide optimal care. In this context, cancer care requires special attention because of its peculiar status by including patients who are commonly immunocompromised and treatments that are often highly toxic. In this review article, we have classified the main impacts of the COVID-19 pandemic on oncology practices –followed by their solutions– into ten categories, including impacts on (1) health care providers, (2) medical equipment, (3) access to medications, (4) treatment approaches, (5) patients’ referral, (6) patients’ accommodation, (7) patients’ psychological health, (8) cancer research, (9) tumor board meetings, and (10) economic income of cancer centers. The effective identification and management of all these challenges will improve the standards of cancer care over the viral pandemic and can be a practical paradigm for possible future crises.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101National Cervical Cancer Screening in Thailand25308941610.31557/APJCP.2021.22.1.25ENPattamaPloysawangNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.JindaRojanamatinNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.SomjitPrapakornNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.PaphawinJamsriNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.ParindaPangmuangNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.KandaSeedaNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.SuleepornSangrajrangNational Cancer Institute, Department of Medical Services, Ministry of Public Health Rama VI road, Ratchathewi Bangkok 10400,
Thailand.Journal Article20201014Background: Cervical cancer is an important public health problem in Thailand. It was the most common cancer in Thai women with the incidence rate of 23.4 per 100,000 women in 1990. Objective: The aim of this study was to share the experiences and summary the outcome of cervical cancer screening program in Thailand. Methods: The Ministry of Public Health in cooperation with the National Health Security Office, launched the National Cervical Cancer Screening Program, covering 76 provinces nationwide under Universal Coverage Scheme in 2005. The screening method are Pap smear and Visual Inspection with Acetic acid (VIA) for women aged 30-60 and 35-45 respectively with a 5-year screening interval. Detecting cervical pre-cancerous lesions will follow by day care treatment such as cryotherapy, Loop Electrosurgical Excision Procedure, etc. Results: The first phase (2005-2009), was carried out on 3,124,855 women, the coverage reached 77.5%. For the second phase (2010-2014), 7,637,226 women were screened, reaching 53.9% coverage of target women. However, we have few data of follow up examination after abnormal screening. Therefore, we conducted new system to get more follow up data in 2019. Under the coordination of many related partners, 10,762,081 women have been screened during 2005-2014. The incidence rate declined to 11.7 per 100,000 women which is ranked as the third most common in women in 2014. Conclusion: This article briefly reviews the challenge of implementing an efficient cervical cancer screening in Thailand. In 2020, HPV testing has been introduced as a primary screening test for all Thai women attending public health sector instead of conventional Pap test.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Tobacco Use and Its Association with Mental Morbidity and Health Compromising Behaviours in Adolescents in Indonesia31358941710.31557/APJCP.2021.22.1.31ENKarlPeltzerDepartment of Research Administration and Development, University of Limpopo, Turfloop, South Africa.SupaPengpidDepartment of Research Administration and Development, University of Limpopo, Turfloop, South Africa.ASEAN Institute for Health Development, Mahidol University, Salaya, Phutthamonthon, Nakhonpathom, Thailand.0000-0002-7714-8869Journal Article20200101Background: Limited evidence has been established on associations between tobacco use and mental morbidity and health compromising behaviours. The study aimed to investigate the associations between tobacco use, mental problems, and health risk behaviour among adolescents attending school in Indonesia. Methods: Nationally representative data were studied from 11,124 adolescents that took part in the cross-sectional “Indonesia Global School-Based Student Health Survey (GSHS) in 2015”. Results: The prevalence of current tobacco use was 12.8%. In adjusted logistic regression analysis, compared to non-current or never tobacco users, current tobacco use was associated with eight of eight mental problem indicators (lonely, anxiety, no close friend, suicidal ideation, suicide plan, suicide attempt and current alcohol use), two of four dietary risk behaviours (soft drink and fast food consumption) and seven of ten other health risk behaviours (in a physical fight, bullied, injury, ever sex, school truancy, and two sub-optimal hand hygiene behaviours). Conclusion: Compared to nontobacco users, current tobacco users had significantly higher mental problem indicators and health risk behaviours. Multiple comorbidity with tobacco use should be targeted in interventions.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Modifiable Factors and Colon Cancer Risk in Thai Population37438941810.31557/APJCP.2021.22.1.37ENSuthatChottanapundBamrasnaradura Infectious Disease Institute, Ministry of Public Health, Nontaburi 11000, Thailand.0000-0001-6630-4339KanitthaChamroonsawasdiDepartment of Family
Health, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand.PravichTunyasitthisundhornMedical Research Network of the Consortium of
Thai Medical Schools, Bangkok 10900, Thailand.WichaiAekplakornFaculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.PimpanSilpasuwanFaculty
of Public Health, Mahidol University, Bangkok 10400, Thailand.PureeAnantachotiDepartment of Social and Administrative Pharmacy, Faculty of
Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand.NipaRojroongwasinkulInstitute of Nutrition, Mahidol University, Nakhon
Pathom 73170, Thailand.SangaDamapongBureau of Health Promotion, Department of Health, Ministry of Public Health, Nonthaburi, 11000,
Thailand.BunditSornpaisarnThai Health Promotion Foundation, Bangkok 10120, Thailand.WiwatRojanapithayakornFaculty of Medicine, Ramathibodi Hospital, Bangkok
10400, Thailand.KumnuanUngchusakBureau of Epidemiology, Nonthaburi 11000, Thailand.Journal Article20200330To demonstrate the possible impact of modifiable factors on colon cancer development in Thai population, we conducted this case-control study from June 2016 until June 2017. The study was conducted in 11 Thai provincial hospitals. The hospitals in this study were selected by stratification by regions. Patients included 504 ones who were newly diagnosed with colon cancer within 1 month. In the control group, 997 health individuals were enrolled. Both case and control were adjusted by age. The results of this study showed that age and socioeconomic factors were associated with colon cancer risk. In addition, it was found that family history of colon cancer had very high association with colon cancer risk. Behavioral factors, including smoking, inadequate physical exercise, and salty food consumption were associated with colon cancer. We detected no association between obesity, alcohol consumption, and colon cancer. The results suggested that colon cancer might have higher association with genetic factors than behavioral factors among Thai patients. Based on the results of this study, stop smoking and promote adequate physical activity are suggested to reduce the incidence of colon cancrr among Thai patients.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Perceived Barriers to Colorectal Cancer Screening among Eligible Adults in Qatar and the Associated Factors: A Cross-Sectional Study45518941910.31557/APJCP.2021.22.1.45ENAymanAl-DahshanDepartment of Medical Education, Community Medicine Residency Program, Hamad Medical Corporation, Doha, Qatar.0000-0001-6132-3223ShaikhaAbushaikhaDepartment of Preventive Screening Program, Primary Health Care Corporation, Doha, Qatar.MohamadChehabDepartment of Medical Education, Community Medicine Residency Program, Hamad Medical Corporation, Doha, Qatar.0000-0002-0862-2264MohamedBalaDepartment of Medical Education, Community Medicine Residency Program, Hamad Medical Corporation, Doha, Qatar.VaheKehyayanUniversity of Calgary in Qatar,
Doha, Qatar.MieaadOmerDepartment of Medical Education, Community Medicine Residency Program, Hamad Medical Corporation, Doha, Qatar.OmaymaAlMohamedDepartment of Clinical Affairs, Primary Health Care Corporation, Doha, Qatar.NooraAl-KubaisiDepartment of Family and
Community Medicine, Primary Health Care Corporation, Doha, Qatar.NagahSelimDepartment of Family and
Community Medicine, Primary Health Care Corporation, Doha, Qatar.Journal Article20200421Background: In Qatar, colorectal cancer (CRC) is the second most common cancer and is projected to be more than triple by 2035. Therefore, CRC periodic screening is vitally important because early detection will improve the success of treatment. In 2016, Qatar established a population-based screening program for CRC targetting average-risk adults. This study aimed to determine the perceived barriers to undergo CRC screening in eligible adults in Qatar and the associated factors. Methods: This was a cross-sectional study of individuals aged 50-74 years who have been never screened, across six primary health centers between September 2018 and January 2019. A non-probability sampling method was used to recruit participants. Participants were interviewed using a structured questionnaire. Descriptive and analytic statistics were applied. Results: A total of 188 individuals participated in the study. The mean age of the participants was 58.3 (SD ±6.4) years. Most participants were females (54.5%) and non-Qatari Arabs (54.3%). The top five reported barriers to CRC screening were: not at risk due to absence of symptoms (60.6%), not at risk due to absence of family history (55.1%), not at risk due to adopting a healthy lifestyle (52.7%), lack of time (41%), and lack of reminders by healthcare workers (39.4%). Bivariate analyses identified statistically significant associations between certain barriers and female gender, nationality, and educational level (primary school and below). Conclusion: The present study identified several barriers to undergoing CRC screening among eligible adults in Qatar. Such results provide a basis for tailoring of future educational campaigns that are relevant, specific, and appealing to such a cohort.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Cytotoxic and Apoptotic Activity of Aglaforbesin Derivative Isolated from Aglaia loheri Merr. on HCT116 Human Colorectal Cancer Cells53608942010.31557/APJCP.2021.22.1.53ENNorielyn N.AbalosInstitute of Biology, University of the Philippines Diliman, 1101, Quezon City, Philippines.Department of Biology, University
of San Carlos-Talamban Campus, 6000, Cebu City, Philippines.0000-0001-9488-8888Virgilio D.Ebajo Jr.NMR Laboratory, Central Instrumentation Facility, De La
Salle University, Laguna Campus, LTI Spine Road, Laguna Boulevard, Barangays Biñan and Malamig, Biñan City, Laguna,
Philippines.Chemistry Department, De La Salle University, 2401 Taft Avenue, 0922 Manila, Philippines.Drexel H.CamachoNMR Laboratory, Central Instrumentation Facility, De La
Salle University, Laguna Campus, LTI Spine Road, Laguna Boulevard, Barangays Biñan and Malamig, Biñan City, Laguna,
Philippines.Chemistry Department, De La Salle University, 2401 Taft Avenue, 0922 Manila, Philippines.Sonia D.JacintoInstitute of Biology, University of the Philippines Diliman, 1101, Quezon City, Philippines.Journal Article20200517Background: The genus Aglaia (Meliaceae) is an established source of many anticancer compounds. The study evaluated the leaf extracts of Aglaia loheri, a tree native to the Philippines, as potential source of anticancer compounds. Methods: Using bioassay-guided fractionation, A. loheri leaf extract was subjected to various chromatographic techniques and step-wise application of MTT assay on human colorectal carcinoma cells, HCT116, to determine the cytotoxic fractions. The most cytotoxic HPLC isolate was structurally identified using 1D and 2D NMR and its apoptotic effect was assessed by JC-1 staining, caspase 3/7 assay and TUNEL assay. Results: After stepwise chromatography fractionation, an HPLC isolate, structurally identified as aglaforbesin derivative (AFD), demonstrated potent cytotoxicity against HCT116. AFD exhibited strong toxicity (IC50 = 1.13 ±0.07 µg/mL) and high selectivity on HCT116 than normal human kidney cells (HK-2). AFD-induced toxicity to HCT116 is possibly through the stimulation of the apoptotic signaling pathway via caspase 3/7 activation and DNA fragmentation independent of mitochondrial membrane depolarization. Conclusion: AFD exhibited selective cytotoxicity and apoptotic activity to HCT116 and could be further developed as anticancer drug lead.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Volumetric-Modulated Arc Radiotherapy with Daily Image-Guidance Carries Better Toxicity Profile for Higher Risk Prostate Cancer61688942110.31557/APJCP.2021.22.1.61ENAhmad IGhanemDepartment of Radiation Oncology, Henry Ford Cancer Institute, Detroit, Michigan, USA.Department of Clinical Oncology, Faculty of Medicine, Alexandria University, Egypt.0000-0002-3396-6763Amr AElsaidDepartment of Clinical Oncology, Faculty of Medicine, Alexandria University, Egypt.0000-0003-0264-1744Mohamed AElshaikhDepartment of Radiation Oncology, Henry Ford Cancer Institute, Detroit, Michigan, USA.Gehan AKhedrDepartment of Clinical Oncology, Faculty of Medicine, Alexandria University, Egypt.0000-0003-3634-3956Journal Article20200601Purpose: To compare radiotherapy-induced toxicity for localized prostate-cancer (PCa) treated with versus without daily image-guidance. Patients and Methods: We identified consecutive intermediate and high-risk localized PCa patients treated with definitive radiotherapy using intensity-modulated radiotherapy (IMRT) with variable duration of androgen-deprivation therapy (ADT) within 2015-2016 (Arm-A) and 2005-2007 (Arm-B). Arm-A cases received daily online imaging guidance (IGRT) using cone-beam computed tomography (CBCT) unlike Arm-B candidates with no daily IGRT. After reporting demographic, clinico-pathological features and treatment details, we compared acute (within 3 months post-therapy) and late RT-induced toxicities between study groups graded by RTOG/CTCAE criteria. Uni/multivariate analyses (UVA/MVA) were performed to identify independent predictors for RT-related side-effects. Results: We were able to identify 257 cases who met our inclusion criteria. Overall, median age was 73 years (48-85), 67% had intermediate-risk and 47% received ADT. Arm-A included 72 patients who received IMRT delivered using volumetric-modulated arc therapy (VMAT), whereas, Arm-B was formed of 185 cases who utilized step-and-shoot static IMRT. Clinico-pathological features and treatment details were non-different across study arms except that Arm-A had more Grade Group 3, higher median total dose (79.2 vs. 74 Gy) and more pelvic lymph-nodes RT (p <0.05). Although acute toxicity was similar across groups, Arm-B encountered higher late toxicity score, more intense late genitourinary side-effects (P=0.008), with non-different late lower-gastrointestinal toxicities. On MVA, lack of daily CBCT, African-American race and higher comorbidities were independently predictive for late toxicities. Conclusion: IMRT with daily CBCT permitted safe delivery of dose-escalated IMRT with improved toxicity profile for higher-risk prostate cancer.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Development and Validation of Questionnaire to Assess Knowledge about Cervical Cancer among Women Aged 20 to 65 years in Oman69748942210.31557/APJCP.2021.22.1.69ENAisha AlSaadiDepartment of Studies and Research, Directorate General of Planning and Studies, Ministry of Health, Muscat, Oman.Journal Article20200603Objective: This study aimed to develop and validate a questionnaire to assess awareness of cervical cancer, its risk factors, and methods of prevention among Arabic-speaking women aged 20 years and over. Methods: The study took place at primary healthcare institutions, Al Buraimi Governorate, Oman, between November 2018 to February 2019. In phase I, seventy items concerning cervical cancer and its prevention were generated through a literature review. In phase 2, the questionnaire was validated through calculating the content validity index (CVI) for both item level (I-CVI) and the scale level (S-CVI), in this phase a shortened English questionnaire of 55 items was formed, then rigorously translated to the Arabic language in phase III. The questionnaire was tested for reliability in two stages: A pilot and a large field test in phase IV. Results: A total of 55 out of 70 items formed the final version of the questionnaire. The final instrument had an S-CVI/Ave of 0.92. The questionnaire called the Knowledge in Cervical Cancer and Prevention Methods 55-items (KCCPM-55). The Cronbach alpha coefficient was 0.940 for the whole questionnaire, and ranged between 0.57 to 0.93 for each of the domains. Test-retest reliability was examined in a subsample of the total participants sample (r = 0.769, p < 0.001). Conclusion: The KCCPM-55 has been successfully developed in the Arabic language and found to be a valid and reliable instrument for assessing the level of knowledge about cervical cancer and prevention methods among women aged 20 to 65 years in Oman.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Efficacy of Quercetin-Sensitized Cisplatin against N-Nitroso-NMethylurea Induced Testicular Carcinogenesis in Wistar Rats75848942310.31557/APJCP.2021.22.1.75ENHend HGMEl-DiastyDepartment of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.HassanEl-SayyadDepartment of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.0000-0001-9770-8985SherifRefaatDepartment of Medical Oncology, Faculty
of Medicine, Mansoura University, Mansoura, Egypt.Heba AEl-GhaweetDepartment of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.Journal Article20200702Background: Testicular cancer is a public health problem. The goal of this study was to demonstrate the efficacy of quercetin treatment on N-nitroso-N-methyl-urea (MNU)-induced testicular carcinogenesis alone or in combination with cisplatin-treatment. Methods: In total 70 adult male albino rats were categorized into six groups, control, quercetin-treatment (10 mg/kg body weight), cisplatin-treatment (2 mg/kg. body weight), cisplatin and quercetin-treatment, MNU-treatment, MNU plus quercetin-treatment and MNU plus quercetin and cisplatin-treatment. Treatment with quercetin and/or cisplatin was performed after 2 months of MNU induced testicular carcinogenesis. The studied groups were euthanized and sacrificed and their testes were examined for gene expression, biochemical, histological and immunohistochemically analysis, inflammation and apoptosis of germ cells. Results: The fertility of the rats subjected to MNU carcinogenesis was impaired following cisplatin and/or quercetin-treatment. Cisplatin-treatment reduced the fertility rate and improved after quercetin-treatment. Quercetin-treatment decreased the sharp increase in RNA expression of BAX and MPO in both cisplatin-toxicated testes and after MNU carcinogenesis induction. In addition, the testicular levels of testosterone and SOD increased in parallel with depletion of MDA, IL-6, AFP and caspase-3 levels in MNU and/or cisplatin-treatment after –quercetin-treatment. The testicular structure of the cisplatin-treated group recovered their dividing germ and sperm differentiation after-quercetin-treatment. While, there was a great appearance of flourishing germ cell of MNU carcinogenesis post quercetin therapy, there was still a lack of sperm differentiation. Conclusion: Quercetin-treatment showed increased cisplatin activity and decreased testicular carcinogenesis due to anti-neoplastic and antioxidant activities.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Aberrant Methylation of Tumour Suppressor Gene ADAM12 in Chronic Lympocytic Leukemia Patients: Application of Methylation Specific-PCR Technique85918942410.31557/APJCP.2021.22.1.85ENAmiraMohamadHuman Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.RoslineHassanDepartment of Hematology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.0000-0003-0493-1390AzlanHusinDepartment of Medicine, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.0000-0003-0899-0443Muhammad FaridJohanDepartment of Hematology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.0000-0001-6344-0220SarinaSulongHuman Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.0000-0001-5369-2707Journal Article20200706Objective: Chronic Lymphocytic Leukemia (CLL) is a common leukemia among Caucasians but rare in Asians population. We postulated that aberrant methylation either hypermethylation or partial methylation might be one of the silencing mechanisms that inactivates the tumour suppressor genes in CLL. This study aimed to compare the methylation status of tumour suppressor gene, ADAM12, among CLL patients and normal individuals. We also evaluated the association between methylation of ADAM12 and clinical and demographic characteristics of the participants. Methods: A total of 25 CLL patients and 25 normal individuals were recruited in this study. The methylation status of ADAM12 was determined using Methylation-Specific PCR (MSP); whereas, DNA sequencing method was applied for validation of the MSP results. Results: Among CLL patients, 12 (48%) were partially methylated and 13 (52%) were unmethylated. Meanwhile, 5 (20%) and 20 (80.6%) of healthy individuals were partially methylated and unmethylated, respectively. There was a statistically significant association between the status of methylation at ADAM12 and the presence of CLL (p=0.037). Conclusion: The aberrant methylation of ADAM12 found in this study using MSP assay may provide new exposure to CLL that may improve the gaps involved in genetic epigenetic study in CLL.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Survival after Laparoscopic versus Abdominal Radical Hysterectomy in Early Cervical Cancer: A Randomized Controlled Trial93978942510.31557/APJCP.2021.22.1.93ENLuciana SilveiraCamposInstituto de Saúde Pública da Universidade do Porto, Universidade do Porto, Portugal.0000-0001-6832-9409Leo FranciscoLimbergerServiço de Ginecologia, Hospital Nossa
Senhora da Conceição, Porto Alegre, Brazil.Airton TeltebomSteinDepartamento de Saúde Pública da Universidade Federal de Ciências da Saúde de
Porto Alegre, Porto Alegre, Brazil.Serviço de Medicina de Família, Hospital Nossa Senhora da Conceição, Porto Alegre, Brazil.0000-0002-8756-8699Jose ManuelCaldasInstituto de Saúde Pública da Universidade do Porto, Universidade do Porto, Portugal.0000-0003-0796-1595Journal Article20200713Background: Previous studies have reported the safety of laparoscopic radical hysterectomy for treatment of early cervical cancer, as option to laparotomy. This study aims to compare overall survival between laparoscopic versus abdominal radical hysterectomy for early cervical cancer. Methods: A single-center randomized controlled trial enrolled 30 patients with clinically staged IA2 cervical cancer and lymphovascular invasion, IB and IIA, who underwent laparoscopic radical hysterectomy (16) or abdominal radical hysterectomy (14). Result: The mean overall survival time was 74.74 months (CI 95%: 54.15-95.33) for LRH 91.67 months (CI 95%: 74.97-108.37) for ARH (log-rank test = 0.30). The mean disease-free survival time was 81.07 months (CI 95%: 60.95-101.19) for LRH and 95.82 months (CI 95%: 80.18-111.47) for ARH (log-rank test = 0.371). The overall survival hazard ratio was 2.05 (CI 95%: 0.51-8.24), and the disease-free hazard ratio was 2.13 (CI 95%: 0.39-11.7). Conclusion: Our study suggests a non-significant trend of worse outcomes for LRH. In light of recent controversy and need for prospective studies, further studies in different populations are required for definite conclusions and until then, patients should be aware of risks and benefits, survival data and quality of life outcomes related to both surgical techniques.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101EZB-ICR Cell Line: A New Established and Characterized Oral Squamous Cell Carcinoma Cell Line From Tongue991038942610.31557/APJCP.2021.22.1.99ENNooshafarinChenariInstitute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.BijanKhademiResearch Center of
Otolaryngology Head and Neck Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Otolaryngology,
School of Medicine, Shiraz University of Medical Sciences,Shiraz, Iran.MahboobehRazmkhahInstitute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Journal Article20200729Background: Tongue cancer is one of the most aggressive forms of oral squamous cell carcinoma which needs more investigations. Herein, we aimed to establish and characterize a tongue cancer cell line. Methods: Tumor tissue was obtained from a 70-year-old woman with tongue cancer. The established cell line named as EZB-ICR and characterized for doubling time, expression of specific markers, HPV corporation and migration status using flow cytometry, immunofluorescence staining, multiplex PCR, and migration assay. Results: EZB-ICR was negative for expression of mesenchymal specific markers, cytokeratin19, pan-cytokeratin, vimentin and EPCAM, but was positive for S100 and Nestin. No appearance of human papilloma virus DNA was seen. The doubling time of EZB-ICR was 31 hours and migration assay confirmed its metastatic nature. Conclusion: To the best of our knowledge, EZB-ICR is the first tongue human cancer cell line established in Iran, and its features make it appropriate for cancer-based in vitro studies and contribute to more studies on tongue cancer.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Spatial Association between Gastric Cancer Mortality and Goiter in Sardinia1051108942710.31557/APJCP.2021.22.1.105ENGiovanni MarioPesDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, University of Sassari, Sassari 07100, Italy.0000-0003-3265-2823GiuseppeFanciulliDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, University of Sassari, Sassari 07100, Italy.Alessandro PalmerioDelitalaDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, University of Sassari, Sassari 07100, Italy.Andrea FaustoPianaDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, University of Sassari, Sassari 07100, Italy.Maria PinaDoreDipartimento di Scienze Mediche, Chirurgiche e Sperimentali, University of Sassari, Sassari 07100, Italy.Baylor College of
Medicine, 77030 Houston, Texas, USA.Journal Article20200814Background: Gastric cancer (GC) is the third leading cause of cancer mortality worldwide. The incidence of GC varies between countries according to exposure to different risk factors. Hypothyroidism has been suggested as a potential GC risk factor. In Sardinia, Italy, the prevalence of endemic goiter is high and GC mortality is unevenly distributed. This ecological study aimed to investigate GC mortality and its relationship with hypothyroidism, adjusting for potential confounders. Methods: The spatial association between GC mortality and goiter (a proxy of hypothyroidism), diet, stature and pastoralism (a proxy of Helicobacter pylori infection), available at the aggregated level, was modelled in the island’s 377 municipalities, separately by sex, using geographically weighted regression (GWR). Results: The GC standardized mortality ratio ranged from 0.0 to 10.4 across municipalities. A hotspot of GC mortality was detected in the central mountainous area of Sardinia among males, positively associated with goiter (GWR estimate 0.213 ± 0.122), and the practice of sheep‒rearing (GWR estimate 0.127 ± 0.080), whereas a negative association with the diet score (GWR estimate 0.032 ± 0.034), and null for stature were found. No significant associations were found in females. Conclusion: Within the limitations of ecological studies goiter prevalence was an independent predictor of GC mortality in males.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Diagnostic Value of Plasma miR-145 and miR-185 as Targeting of the APRIL Oncogene in the B-cell Chronic Lymphocytic Leukemia1111178942810.31557/APJCP.2021.22.1.111ENMaliheBagheriDepartment of Biotechnology and Molecular Medicine, Arak University of Medical Sciences, Arak, Iran.BehzadKhansarinejadDepartment of
Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran.GhasemMosayebiDepartment of
Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran.AlirezaMoradabadiDepartment of Hematology and Blood Banking,
Arak University of Medical Sciences, Arak, Iran.MahdiehMondanizadehDepartment of Biotechnology and Molecular Medicine, Arak University of Medical Sciences, Arak, Iran.Molecular and Medicine Research Center, Arak University of Medical Sciences,
Arak, Iran.Journal Article20200901Background: Chronic lymphocytic leukemia (CLL) is one of the most common hematologic malignancy in adults worldwide. This cancer has a poor prognosis at different stages. So, the identification of new biomarkers is important for diagnosis of B-CLL. Considering the oncogenic role of APRIL molecule in this leukemia as well as the regulatory role of miRNAs in different signaling pathways, the present study evaluated the miRNAs targeting APRIL gene in B-CLL. Methods: The miRNAs were predicted and selected using bioinformatics algorithms. A total of 80 plasma samples were subjected to RNA extraction and synthesis of cDNA. The expressions levels of predicted miRNAs and APRIL gene in plasma of B-CLL patients and healthy individuals were assessed by Real time PCR analysis. ROC analysis was performed to investigate the role predicted miRNAs as novel biomarkers in diagnosis of B-CLL. Results: The results of the prediction showed that miR-145-5p and miR-185-5p target the APRIL gene. The expression level of APRIL gene was strikingly higher in plasma of B-CLL patients than in the healthy individuals (102, P= 0.001). On the other hand, expression levels of miR-145-5p and miR-185-5p were strikingly lower in B-CLL patients than in the healthy individuals (0.07, P= 0.001) (0.29, P= 0.001). Also, ROC curve analyses demonstrated that miR-145-5p and miR-185-5p are specific and sensitive and may serve as new biomarkers for the detection of B-CLL. (AUC; 0.95, sensitivity; %90) (AUC; 0.87, sensitivity; %63). Conclusion: These data suggest that miR-145-5p and miR-185-5p target the APRIL gene and might have a role in diagnosis of B-CLL. Therefore, these two miRNAs can be served as a novel and potential biomarker for detection of B-CLL.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Association between Angiotensin-Converting Enzyme- Insertion/Deletion Polymorphism and Diabetes Mellitus-2 in Saudi Population1191238942910.31557/APJCP.2021.22.1.119ENMahmoudHabibullahDepartment of Medical Laboratory Technology, Faculty of Applied Medical Science, Jazan University, Saudi Arabia.0000-0002-4155-3379FarhanaAkterDepartment
of Medicine, University Of Cambridge, United Kingdom.XingpingQinMassachusetts General Hospital Cancer Centre, Harvard Medical
School, USA.MohtashimLohaniEmergency Medical Services, Jazan University, Saudi Arabia.Mohammed SAldughaimKing Fahad Medical City, Riyadh, Saudi Arabia.YahiaAl-KaabiDepartment of Medical Laboratory Technology, Faculty of Applied Medical Science, Jazan University, Saudi Arabia.0000-0001-5418-5782Journal Article20200912Objectives: The association of angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism and the development of type 2 diabetes mellitus (T2DM) has been debated vigorously but still remains controversial. Therefore, the current study was designed to determine the possible association between ACE I/D polymorphism and T2DM and hypertension (HTN) in a population of Saudi Arabian participants. Methods: A total of 143 individuals were recruited for the study, consisting of 74 controls and 69 patients with T2DM. Genotyping was performed via polymerase chain reaction. Results: The genotype frequencies for DD, ID and II in controls were 52.7%, 39.2% and 8.1%, whereas in T2D patients it was 52.2%, 40.6% and 7.2% respectively. The DD frequency was highest out of the three genotypes in both the controls and the T2DM patients. Conclusion: There was no significant difference found in the genotype and allele frequencies between cases and controls, suggesting that insertion/deletion polymorphism in the ACE gene may not be associated with an increased susceptibility to type 2 diabetes in our study population.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Saliva as a Potential Diagnostic Tool to Evaluate Relationship between Oral Microbiome and Potentially Malignant Disorders for Prevention of Malignant Transformation1251298943010.31557/APJCP.2021.22.1.125ENSandhya SinghKushwahaDepartment of Oral Pathology and Microbiology, Faculty of Dental Sciences, King George Medical University, Lucknow, Uttar
Pradesh, India.Rupinder KaurMultaniDepartment of Oral Pathology and Microbiology, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh,
India.Narendra SinghKushwahaDepartment of Prosthodontics, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh, India.SwatiGautamDepartment of Oral Pathology and Microbiology, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh,
India.Deepti GargJindalDepartment of Oral Pathology and Microbiology, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh,
India.Karandeep SinghAroraDepartment of Oral
Medicine and Radiology, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh, India.0000-0003-1753-2305AvijitAvasthiDepartment of Public Health
Dentistry, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh, India.Journal Article20200910Objective: The current research was conducted with an aim to assess the association of oral microbiome with Potentially malignant disorders (PMDs) because usage of tobacco in any form alters the normal microbiome and shifts it towards dysbiosis. Thus, our definitive knowledge of the oral commensal bacteria and oral cancer link can definitely be used as a potential adjunct to early diagnosis and management of PMDs and prevent it’s malignant transformation. Study Design: A total of 100 individuals of minimum 18 years of age were included in the study which, were classified into 2 groups of tobacco users (50) and non-tobacco users (50). The tobacco users had a history of tobacco consumption for at least 5 years. Results: The present study, showed highest percentage (72%) of anaerobic bacteria, followed by aerobic (22%) and lowest count of yeast (4%). Conclusion: The ecological shift to dysbiosis is a significant finding in oral carcinogenesis. Further investigation on a larger group of altered microbiomes will definitely help in establishing relationship of altered microbiome and PMDs, which can help in appropriate treatment and better prognosis.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Cardiac, Hepatic and Renal Dysfunction and IL-18 Polymorphism in Breast, Colorectal, and Prostate Cancer Patients1311378943110.31557/APJCP.2021.22.1.131ENGovandQaderDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.MukhlisAaliDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Shukur WSmailDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Department of Medical Analysis,
Faculty of Science, Tishk International University, Erbil, Iraq.KazhanMahmoodDepartment of Midwifery, College of Nursing, Hawler Medical
University, Erbil, Kurdistan Region, Iraq.BestoonHasanDepartment of Cancer Registry, Cancer Control Unit, Erbil Directorate of Health, Erbil,
Iraq.KarwanM-AmenDepartment of Medical Analysis,
Faculty of Science, Tishk International University, Erbil, Iraq.Department of Nursing, College of Nursing, Hawler Medical University, Erbil, Kurdistan Region, Iraq.Dlzar BayzRahmanInternal Laboratory,
Hawler Teaching Hospital, Erbil Directorate of Health, Erbil, Iraq.Fikry AQadirDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Dara KMohammadCollege of Agricultural Engineering Sciences, Salahaddin
University-Erbil, Erbil, Kurdistan Region, Iraq.Center for Hematology and Regenerative Medicine (HERM), Department of
Medicine Huddinge, Karolinska Institutet, 141 83 Stockholm, Sweden.Hastyar HNajmuldeenDepartment of Biology, College of Science, University of
Sulaimani, Kurdistan Region, Iraq.Medical Laboratory Analysis, Cihan University-Sulaimaniya, Slemani, Iraq.Fryad MajeedRahmanDepartment of Biology, College of Science, University of
Sulaimani, Kurdistan Region, Iraq.Seepal IbrahimAhmadEmergency
Hospital, Duhok General Health Directorate, Duhok, Kurdistan Region, Iraq.Nergz SSalihDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Zainab MKhdhrDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Bushra AMohammedDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Asuda MMajeedDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Xanda MHasanDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Bushra HKhidhirDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Eman SMuhammadDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Bahar AMuhamadsalihDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Simav K.HasanDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Aram JHamadDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Zahra KEsmailDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Chra MIsmaelDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Shan MHusaenDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Chiavan AAbdullaDepartment of Pathological Analysis, Faculty of
Science, University of Knowledge, Erbil, Kurdistan Region, Iraq.Bashdar MHussenCollege of Pharmacy, Hawler Medical University, Kurdistan
Region, Iraq.ZjwanHouseinDepartment of Medical Laboratory Technology, Health Technical College, Erbil Polytechnic University, Erbil, Iraq.MudhirShekhaDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.AbbasSalihiDepartment of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq.Department of Nursing, College of Nursing, Hawler Medical University, Erbil, Kurdistan Region, Iraq.0000-0002-2672-5785Journal Article20200910Introduction: The present study aimed to determine the alterations in the serum levels of tumor markers used to evaluate cardiac, renal and liver function, and detect the interleukin (IL)-18 rs1946518 polymorphism in breast (BC), colorectal (CRC) and prostate cancer (PCa) patients. Methods: Blood samples were collected from 65 female BC, 116 CRC, 79 PCa and 88 myocardial infarction (MI) patients, and 110 healthy individuals to determine the concentration of tumor and cardiac markers. Furthermore, the IL-18 rs1946518 polymorphism was assessed using amplification refractory mutation system (ARMS)-PCR. Results: The serum levels of the tumor markers cancer antigen 15-3 (CA 15-3), carbohydrate antigen 19-9 (CA 19-9), carcinoembryonic antigen (CEA) and total prostate-specific antigen (TPSA) were significantly increased in cancer patients compared with healthy controls. Furthermore, the activity of high-sensitivity cardiac troponin T (hs-cTnT) and creatine kinase‑myocardial band (CK-MB) was enhanced in MI patients, however, their activity was unchanged in cancer patients. The activity of alkaline phosphatase (ALP), and the serum concentration of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and urea were markedly elevated in CRC and PCa patients, respectively, compared with the control group. Although, no significant differences were observed in the -607 C/A polymorphism and allele frequency of IL-18 among BC, CRC patients and healthy individuals, the odds ratio (OR) was 1.75 for both C and A allele in BC patients. Therefore, the -607 C/A polymorphism could be considered as a risk factor for BC. Conclusion: The aforementioned results suggested that tumor markers could be considered as excellent biomarkers for the early detection of BC, CRC and PCa, whereas the concentration of liver enzymes could serve as an alternative indicator for the diagnosis of CRC and PCa. Additionally, the rs1946518 polymorphism in the IL-18 gene could be considered as a risk factor for the occurrence of BC, CRC and PCa.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Analysis of Curcumin as a Radiosensitizer in Cancer Therapy with Serum Survivin Examination: Randomised Control Trial1391438943210.31557/APJCP.2021.22.1.139ENYudi MulyanaHidayatDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.0000-0002-8010-5907FrankWageyDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.DodiSuardiDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.0000-0003-3084-8101HermanSusantoDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.Bismarck JLaihadDepartment of Obstetrics and Gynecology, School of Medicine, Universitas Sam Ratulangi, Manado, Indonesia.Maringan Diapari LumbanTobingDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjajaran, Bandung, Indonesia.Journal Article20200915Objective: One of the important treatments for cervical cancer is radiation therapy. This study sought to determine the role of curcumin as a radio-sensitizing agent for use with radiation therapy for cervical cancer. To accomplish this, we assessed the levels of survivin, which is an anti-apoptotic protein that plays a role in cell division and apoptosis inhibition. Method: This study used a quasi-experimental design, including a pretest–posttest control group design approach. The study subjects included cervical carcinoma stage IIB–IIIB patients who were scheduled to undergo surgery at the Hasan Sadikin Hospital Bandung during the research period. The advanced cervical cancer patients were assigned to two groups: i) those who received curcumin + radiation therapy and ii) those who received placebo + radiation therapy. Results: In the group treated with curcumin + radiation, 15 (75%) patients showed decreased survivin levels and 5 (25%) showed increased survivin levels. Whereas, in the placebo + radiation group, there were 8 (40%) patients who showed decreased survivin levels and 12 (60%) who showed increased survivin levels. Conclusion: In conclusion, curcumin is an effective, alternative radiosensitizer agent for application in cervical cancer treatment.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Predictive Factors Increasing the Risk of Radiation Toxicity in Patients with Early Breast Cancer1451498943310.31557/APJCP.2021.22.1.145ENAsmaa AAbdeltawabDepartment of Clinical Oncology, Faculty of Medicine, Assiut University, Assiut, Egypt.0000-0003-0607-7386Samia AAliDepartment of Clinical Oncology, Faculty of Medicine, Assiut University, Assiut, Egypt.Hanan GMostafaDepartment of Clinical Oncology, Faculty of Medicine, Assiut University, Assiut, Egypt.Mohamed AHassanDepartment of Clinical Oncology, Faculty of Medicine, Assiut University, Assiut, Egypt.Journal Article20200917Objectives: Radiation induces adverse events on healthy tissues which may be augmented by certain factors. This study aimed to assess patients; tumor and treatment-related factors which increase the risk of radiation-induced toxicity in breast cancer patients. Methods: This prospective study included postmenopausal early breast cancer patients treated at the clinical oncology department, Assiut University, Egypt between January 2015 and December 2018. Patients treated with mastectomy followed by conventional radiotherapy (25x 2 Gy) and either concurrent or sequential letrozole. Acute and late radiation toxicity was scored according to EORTC/RTOG and risk factors were analyzed. Results: A total of 75 patients were included in the study. After a median follow-up of 24 months, 12 patients had > grade 2 acute dermatitis, 5 patients had > grade 2 cardiac toxicity and 3 patients had > grade 2 lung toxicity. Multivariate analysis revealed that trastuzumab use was associated with a decrease risk of acute dermatitis (p= 0.01) but boost irradiation was significantly associated with increased risk of acute dermatitis (p= 0.01). Late toxicity > grade 2 was observed in 6 patients, 14 patients, and 2 patients for skin, heart, and lung respectively. Conclusion: The use of boost irradiation was associated with increased risk of acute dermatitis, in the contrary; the use of trastuzumab seemed to be protective as observed in this study.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101The Evaluation of Cytotoxic Properties from CCB-2 Sugar Complexes Against TNBC and Non-TNBC Cells1511558943410.31557/APJCP.2021.22.1.151ENRiris IstighfariJenieLaboratory of Macromolecular Engineering, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.0000-0002-0230-6260Rohmad YudiUtomoCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.Laboratory of Medicinal Chemistry, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.0000-0003-4803-9417Ratna AsmahSusidartiCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.Laboratory of Medicinal Chemistry, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.0000-0002-0973-441XDhaniaNovitasariCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.0000-0002-5661-8841MitsunoriKirihataResearch Center of Boron Neutron Capture Therapy, Osaka Prefecture University, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka, Japan.EdyMeiyantoLaboratory of Macromolecular Engineering, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara II, 55281 Yogyakarta, Indonesia.0000-0002-0886-6322Journal Article20200918Objective: The progress from Boron Neutron Capture Therapy (BNCT) development urged us to explore new targeted and selective boron carriers. Firstly, we reported the successful synthesis of CCB-2 which exerts a cytotoxic effect against triple negative breast cancer (TNBC) cells. We introduced the new modification of CCB-2 with sugar and alcohol sugars to enhance its solubility in hoping to increase cellular uptake. Methods: CCB-2 fructose complex (CCB-2-F), CCB-2 sorbitol complex (CCB-2-Sor), and CCB-2 xylitol complex (CCB-2-Xy) were obtained with small size within nano-specific particle. All the compounds were then determined for their cytotoxic activities through MTT assay. Results: All compounds were performed cytotoxic activities against TNBC 4T1 and HER-2 positive MCF-7/HER2 cells with good selectivity when tested in immortalized fibroblast cells. Conclusion: Overall, we provided a new modification of CCB-2 through complexation with sugars. Still, further evaluations are needed to develop more efficient CCB-2 as the new candidate of anticancer agent, notably in breast cancer.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Role of miR153 and miR455-5p Expression in Oral Squamous Cell Carcinoma Isolated from Plasma1571618943510.31557/APJCP.2021.22.1.157ENSajadBaberDepartment of Oral and Maxillofacial Surgery, School of Dentistry, International Campus, Tehran University of Medical
Sciences, Tehran, Iran.MohamadBayatDepartment of Oral and Maxillofacial Surgery, School of Dentistry, International Campus, Tehran University of Medical
Sciences, Tehran, Iran.Craniomaxillofacial Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran,
Iran.AbdolrezaMohamadniaChronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD),
Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Biotechnology, School of Advanced Technologies
in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.AhmadrezaShamshiriResearch Center for Caries Prevention, Dentistry
Research Institute, Department of Community Oral Health, School of Dentistry, Tehran University of Medical Sciences, Tehran,
Iran.PouyanAmini ShakibDepartment of pathology School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.NaghmehBahramiCraniomaxillofacial Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran,
Iran.Department of Tissue
Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences,
Tehran, Iran.0000-0002-3575-4094Journal Article20200919Background: Despite the notable advances in modern surgery and radiotherapy,no significant increase in the five year survival rate of oral squamous cell carcinoma has been reported. Collecting evidence demonstrates that miR 153 and miR 455-5p play a key role in growth and progression of oral cancer. Early detection of OSCC is important for enhancing patient quality of life and clinical treatment.For this reason, biomarkers or tumour markers offer an opportunity to intervene and avoid development of oral cancer. Methods: A total of 50 blood samples from patients from both genders (25 OSCC and 25 healthy people/control groups) were obtained to determine the expression of miR153 and miR455-5p using Real time Polymerase chain reaction and t test. Results: In general by using the formula Δ ct, it is evident that the miR 153 expression in peripheral blood is lower in patients than in healthy individuals (1.97) while the miR 455-5p expression in peripheral blood is higher in patients than in healthy individuals (2.56). Conclusion: We conclude that miR153 and miR 455-5p expression in serum can function as a diagnostic screening test for the early detection of oral squamous cell carcinoma.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Factors of Never Screened with Faecal Occult Blood Test in Public Primary Care Facilities1631698943610.31557/APJCP.2021.22.1.163ENMohd FazeliSazaliDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.Syed SharizmanSyed Abdul RahimDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.0000-0002-9090-2563RichardAvoiDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.Mohd RohaizatHassanDepartment of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia, Malaysia.FirdausHayatiDepartment of Surgery, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia.Zahir IzuanAzharDepartment of Public Health Medicine, Faculty of Medicine, Universiti Teknologi MARA, Malaysia.0000-0002-7505-0438Mohammad SaffreeJeffreeDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.KhamisahAwang LukmanDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.NaingOo ThaDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.HelmySajaliDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.AzmanAtilDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.Muhammad AklilAbd RahimDepartment of Community Health and Family Medicine, Faculty Medicine and Health Sciences, Universiti Malaysia Sabah, Malaysia.Journal Article20200925Background: Colorectal cancer (CRC) is still a major public health threat. In the effort to reduce CRC incidence and mortality, faecal occult blood test (FOBT) is currently the screening tools used for early detection of CRC. However, the uptake of FOBT screening is less than promising. This study aims to identify the prevalence and predictors of Never Screened with FOBT (NS-FOBT). Methods: A cross sectional study was conducted in five health clinics under Kota Kinabalu district, Sabah, Malaysia Borneo involving 162 attendees with age of 50 years old and above. A validated self-administered questionnaire was used to collect the data. Multiple logistic regression analysis was used to determine the predictors of NS-FOBT. Results: The prevalence of NS-FOBT was 85.8% (n=139). Important predictors of NS-FOBT were age (aOR: 0.922; 95% CI: 0.855, 0.995; p=0.035), Bumiputera ethnicity (vs Non Bumiputera; aOR: 4.285; 95% CI: 1.384, 13.263; p=0.012), knowledge score (aOR: 0.921; 95% CI: 0.856, 0.99; p=0.027), and attitude score (aOR: 0.801; 95% CI: 0.702, 0.913; p=0.001). Conclusion: There is high prevalence of NS-FOBT. Age, ethnicity, knowledge, and attitude were important predictors of NS-FOBT. Strategies are needed to improve FOBT screening rate among the public. Socio-culturally tailored health promotion strategies as well as strengthening the communication, collaboration, and education to enhance the role of family physician is vital in improving the CRC prevention and care.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Analysis of Predictors of Breast Cancer Screening among Japanese Women using Nationally Representative Survey Data, 2001–20131711788943710.31557/APJCP.2021.22.1.171ENTasukuOkuiMedical Information Center, Kyushu University Hospital, Fukuoka City, Japan.0000-0001-5098-8502Journal Article20201004Objective: Predictors of participation in breast cancer screening in recent years or the trend of participation rate by predictors over the years has not been investigated in Japan. In this study, we investigated predictors for participation in breast cancer screening and analyzed the trend of participation rate depending on the predictors using nationally representative survey data in Japan. Methods: The data of “Comprehensive Survey of Living Conditions” in Japan from 2001 to 2013 were used. Participation in breast cancer screening was used as an outcome. Next, as explanatory variables, we used age group, marital status, living arrangements, educational level, household income, employment status, smoking status, regular outpatient visit status, and self-rated health status. Then, the participation rate for breast cancer screening was calculated for each of the factors over the years. In addition, multivariate logistic regression analysis was conducted to analyze the association between each factor and the participation rate using data from 2010 and 2013. Results: We found that non-married women, women with lower educational level, women with low household income, self-employed or unemployed women, smokers, and women with low self-rated health status were significantly less likely to participate in breast cancer screening. Conversely, the participation rate increased for all predictor groups from 2001 to 2013, and the increase in the participation rate for never-married women was particularly evident compared with the other marital statuses. However, significant differences in the participation rate for breast cancer screening existed depending on marital status, household income, employment status, and smoking status throughout the analyzed years. Conclusion: Our findings suggest that further recommendations for breast cancer screening are particularly needed among women of low socioeconomic status and those who are self-employed or unemployed to increase the participation rate in Japan.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101The Relationship between Perceived Self-Efficacy and Cervical Cancer Screening among Village Health Volunteers in Suratthani Province, Thailand1791838943810.31557/APJCP.2021.22.1.179ENOrnumaBunkarnSchool of Nursing, Walailak University, Thasala, Nakhon Si Thammarat, Thailand.KiatkamjornKusolSchool of Nursing, Walailak University, Thasala, Nakhon Si Thammarat, Thailand.Journal Article20201004Objective: This research aimed to examine the relationship between perceived self-efficacy and cervical cancer screening of village health volunteers. Methods: The researchers conducted this descriptive research with 279 samples, each recruited using the stratified random samplings. Data were collected in August 2020 using two research instruments included general data and the self-efficacy assessment. The self-efficacy assessment was tested, yielding a reliability score of 0.90. Data were then analyzed using descriptive statistics and point- biserial correlation. Results: The results revealed that the mean scores of perceived self-efficacy were at a high level in (Mean= 4.35, S.D.= 0.77) and perceived self-efficacy of each has the mean scores of at a high level in strength, generality, and magnitude dimension (Mean= 4.27, S.D.= 0.76; Mean= 4.40, S.D.= 1.01; Mean= 4.35, S.D.= 0.76) respectively. There were significantly positive correlations between the perceived self-efficacy and cervical cancer screening at a very high level (r= 0.81, p <0.001). The relationship between the perceived self-efficacy of each in the strength dimension and magnitude dimension with cervical cancer screening was at a very high level of relationship significantly (r= 0.84, 0.82, p < 0.001). The generality dimension was at a high level of relationship significantly. (r= 0.66, p < 0.001). Conclusion: This study showed that village health volunteers with high perceived self-efficacy correlated with their confidence screening for cervical cancer. Therefore, village health volunteers should emphasize that women learn and acknowledge the importance of obtaining cervical cancer screening to prevent cervical cancer effectively. <br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Data Integrity of Radiology Images Over an Insecure Network Using AES Technique1851938943910.31557/APJCP.2021.22.1.185ENPavithraPrabhuDepartment of Computer Science and Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, India.0000-0001-7891-9066ManjunathK NDepartment of Computer Science and Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, India.0000-0001-8239-4047ChitraRajaramaDepartment of Information Science and Engineering, NIE Institute of Technology, Mysuru, India.0000-0002-0763-6840AnjaliKulkarniIndependent Consultant, AI in Radiation Oncology, Bengaluru, India.RajendraKuradyResearch and Development, RTWO Healthcare Private LLP, Mahalakshmipuram, Bengaluru, India.Journal Article20201006Background: While transmitting the medical images in radiology information systems the adversary effect can break the CIA (Confidentiality, Integrity, and Availability) triads of information security. The objective of the study was to transmit the complete set of image objects in a dataset without data integrity violation. Methods: In this paper a hybrid cryptographic technique which combines the prime details from the patient dataset (stack of axial 2D images) and the Advanced Encryption Standard (AES) method has been proposed. The steps include a) Creating an artificial X-ray image (DRR) from the 3D volume, b) dividing the DRR image in x and y directions equally into four regions, c) applying the zig-zag pattern to each quadrant, and d) encryption of each quadrant with block cipher mode using the AES algorithm. After dataset transmission the DRR image was regenerated at the receiver and compared each of the deciphered blocks (transmitted ones) using the histogram technique. Results: The technique was tested on CT and MRI scans of sixty datasets. The image injection techniques, such as adding and deleting an image from the dataset and modifying the image pixels, were tested. The results were validated statistically using mean square error and histogram matching techniques. Conclusion: The combination of the DRR and the AES technique has ensured the secured transmission of the entire dataset and not an individual image.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Comparing the Incidence of Buccal Mucosa Cancer in South Asian, White, and Black Populations Residing in the United States: A Cross-Sectional Analysis1951998944010.31557/APJCP.2021.22.1.195ENStephen JSozioRutgers Cancer Institute of New Jersey, Department of Radiation Oncology, New Brunswick, NJ, USA.0000-0002-3641-135XSachinJhawarThe Ohio State University
Comprehensive Cancer Center, Columbus, OH, USA.YaqunWangRutgers School of Public Health, Department of Biostatistics and
Epidemiology, Piscataway, NJ, USA.MutlaySayanRutgers Cancer Institute of New Jersey, Department of Radiation Oncology, New Brunswick, NJ, USA.RahulParikhRutgers Cancer Institute of New Jersey, Department of Radiation Oncology, New Brunswick, NJ, USA.SungKimRutgers Cancer Institute of New Jersey, Department of Radiation Oncology, New Brunswick, NJ, USA.Journal Article20201007Background: Recreational use of the betel nut, which is common among the South Asian population, is a known risk factor for developing Head and Neck cancer. As South Asians comprise a significant proportion of the United States population, we seek to determine if those living within the country experience a higher rate of head and neck cancers compared to other races. Methods: Data of patients diagnosed with head & neck cancers from 2010-2016 was collected from the National Cancer Database® and compared to race-matched US census data for each corresponding year to calculate incidence. Pairwise comparisons were performed between the incidence for South Asians versus Whites and South Asians versus Blacks using one sided Chi-square tests. Results: South Asians experienced a significantly higher incidence of buccal mucosa/vestibule cancers when compared to Whites or Blacks for every year between 2010-2016, but a comparatively lower incidence of larynx or oropharynx cancers. Conclusions: South Asians residing within the United States have a higher incidence of buccal mucosa/vestibule cancers, but a lower incidence of more common cancers, such as larynx or oropharynx cancer. This may suggest that the etiology behind the high buccal mucosa/vestibule cancer incidence is due to a social habit, as opposed to an inherent racial susceptibility.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Association between Genetic Polymorphisms of miR-1307, miR- 1269, miR-3117 and Breast Cancer Risk in a Sample of South East Iranian Women2012088944110.31557/APJCP.2021.22.1.201ENSahelSarabandiDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.HediehSattarifardDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.MohammadKiumarsiDepartment of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.ShimaKaramiDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.MohsenTaheriGenetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.orcid.org/0000-0002-1110-4417MohammadHashemiDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.GholamrezaBahariDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.Children and Adolescent Health Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.SaeidGhavamiDepartment
of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba,
Winnipeg, MB R3E 3P4, Canada.Faculty of Medicine, Katowice School of Technology, 40-555 Katowice, Poland.Autophagy Research Center, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Journal Article20201011Introduction: MicroRNAs (miRNAs) play an essential role in the susceptibility and development of cancer cells. Objective: Examining the dependency of breast cancer risk with genetic polymorphisms of miR-1307, miR-1269, and miR-3117 in a sample of Iranian women (southeast region). Methods: The case-control study consisted of 520 individuals (260 diagnosed BC patients, 260 healthy individuals). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for genotyping of miR-1307 rs7911488, miR-1269 rs73239138, and miR-3117 (rs4655646 and rs7512692) polymorphisms. Results and Conclusion: This study provided evidence that miR-1307 rs7911488 polymorphism significantly reduced the risk of BC in heterozygous AG genotype, as well as dominant (AG+GG) genotype and G allele. A significant correlation was found between dominant (AA+AG) genotype, the A allele and protection against BC due to miR-1269 rs73239138 in the sample of study. In contrast, our findings suggested that AG genotype and G allele of miR-3117 rs4655646 polymorphism could increase BC’s susceptibility among the southeastern Iranian females. The miR-3117 rs7512692 variant also increased the risk of BC in codominant, dominant and recessive models, as well as the T allele. The possible dependency of miR-1307, miR-1269, and miR-3117 variants with patients’ clinicopathological characteristics and BC was also studied. It was concluded that there is a correlation between miR-3117 rs7512692 variant and tumor grade (p=0.031); also, a correlation between miR-1269 rs73239138 variant and progesterone receptor status (p=0.006). The current investigation revealed that miR-1307, miR-1269, and miR-3117 polymorphisms might play a crucial role in the Iranian population’s vulnerability to BC.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Anticancer Activity of Fucoidan via Apoptosis and Cell Cycle Arrest on Cholangiocarcinoma Cell2092178944210.31557/APJCP.2021.22.1.209ENPathaninChantreeDivision of Anatomy, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand.Research Unit in Nutraceuticals and Food Safety, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand.0000-0003-4974-9584KesaraNa-BangchangGraduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, 12120, Thailand.Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma, Thammasat University, Pathumthani, 12120, Thailand.0000-0001-6389-0897PongsakornMartvisetCenter of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma, Thammasat University, Pathumthani, 12120, Thailand.Division of Parasitology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand.0000-0003-4168-5950Journal Article20201012Objective: Many previous studies reported that fucoidan has antitumor activities. The objective of the present study was to determine the cytotoxic effects and related mechanisms of cell death induced by fucoidan extracted from Fucus vesiculosus on CL-6 cholangiocarcinoma cell. Methods: CL-6 and OUMS cells were treated with 0, 100, 200, and 300 μg/mL of fucoidan. MTT assay was used to determine cytotoxicity. Flow cytometry-based assay was used to examine the distribution of apoptosis and cell cycle. The changes in nuclear morphology were determined using Hoechst 33,342 staining. Mitochondrial membrane potential (ΔΨm) was evaluated using the JC-1 kit. The apoptotic, anti-apoptotic, and cell cycle-related proteins study were examined by Western blot analysis. Results: The relative viable cell number of treated CL-6 cells was decreased but no effect was observed in OUMS normal cells. Furthermore, treated cells were arrested in the G0/G1 phase with down-regulation of cyclin D1 and CDK4. Annexin V/PI staining with flow cytometry analysis suggested that fucoidan could induce apoptosis in CL-6 cells. Western blot study revealed the up-regulation of apoptotic markers including Bax, cleaved PARP, cleaved caspase-3, but down-regulation of anti-apoptotic markers, cl-2. Moreover, fucoidan could induce nuclear fragmentation and chromatin condensation with alteration of ΔΨm. Conclusion: Fucoidan exerts antitumor properties against CL-6 cholangiocarcinoma cells illustrated by the induction of apoptosis and cell cycle arrest.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Cucurbitacin B Diminishes Metastatic Behavior of Cholangiocarcinoma Cells by Suppressing Focal Adhesion Kinase2192258944310.31557/APJCP.2021.22.1.219ENPutthapornKaewmeesriDepartment of Pharmacology, Faculty of Medicine, and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon
Kaen, Thailand.VeerapolKukongviriyapanDepartment of Pharmacology, Faculty of Medicine, and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon
Kaen, Thailand.0000-0001-5867-9093AuemduanPrawanDepartment of Pharmacology, Faculty of Medicine, and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon
Kaen, Thailand.0000-0001-6713-567XSarinyaKongpetchDepartment of Pharmacology, Faculty of Medicine, and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon
Kaen, Thailand.0000-0001-9767-4906LaddawanSenggunpraiDepartment of Pharmacology, Faculty of Medicine, and Cholangiocarcinoma Research Institute, Khon Kaen University, Khon
Kaen, Thailand.0000-0001-5509-8664Journal Article20201013Objective: Cholangiocarcinoma (CCA) is a malignant tumor with aggressive metastatic property resulted from dysregulation of metastasis-regulated signaling pathways. The aim of this study was to investigate the effect of cucurbitacin B on metastatic behavior of CCA cells through modulation of focal adhesion kinase (FAK) protein. Methods: KKU-452 cells were treated with a specific FAK inhibitor, FAK inhibitor-14, or cucurbitacin B at various concentrations for 24 h. Cell viability was assessed by sulforhodamine B assay. The migratory and invasive abilities of the cells were investigated using wound healing and transwell invasion assays, respectively. The fibronectin-coated plate was used for adhesion assay. The effects of the test compounds on FAK activation and the expression of metastasis-associated proteins were determined by Western blot analysis. The amount of MMP-9 was evaluated using a commercial ELISA Kit. Results: FAK inhibitor-14 and cucurbitacin B at concentrations which minimally affected KKU-452 cell viability could suppress FAK activation, evidently by decreased level of phospho-FAK protein after exposure to the compound. At these conditions, cucurbitacin B suppressed metastatic behavior including migration, invasion and adhesion abilities of CCA cells similar to FAK inhibitor-14. Further molecular studies demonstrated that FAK inhibitor-14 and cucurbitacin B downregulated the expression of metastasis-associated proteins including MMP-9, ICAM-1 and VEGF. Consequently, exposure to cucurbitacin B inhibited the production of MMP-9 enzyme in CCA cells similar to FAK inhibitor-14 treatment. Conclusion: FAK participated in regulation of metastatic behavior of KKU-452 CCA cells. Cucurbitacin B suppressed FAK activation in the cells which was associated with inhibition of metastasis essential steps and their related metastatic proteins. The compound may be developed as a novel therapeutic agent for CCA metastasis therapy.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Two Novel Mutations of the NPM1 Gene in Syrian Adult Patients with Acute Myeloid Leukemia and Normal Karyotype2272328944410.31557/APJCP.2021.22.1.227ENIsmael FAlarbeedDepartment of Microbiology, Hematology and Immunology, Faculty of Pharmacy, Damascus University, Ministry of High
Education, Damascus, Syria.AbdulsamadWafaDepartment of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria.FatenMoassassDepartment of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria.BasselAl-HalabiDepartment of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria.WalidAlachkarDepartment of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria.ImadAboukhamisDepartment of Microbiology, Hematology and Immunology, Faculty of Pharmacy, Damascus University, Ministry of High
Education, Damascus, Syria.Journal Article20201026Objective: Somatic mutations in exon 12 of the NPM1 gene is one of the most common genetic abnormalities in adult acute myeloid leukemia (AML), which is observed in 25-35% of AML patients and in 50-60% of patients with cytogenetically normal AML (CN-AML). Methods: We performed Sanger sequencing of exon 12 of the NPM1 gene, on 44 CN-AML patients to characterize NPM1 status. Results: In this study, NPM1 mutations were identified in 10 (22.7%) of the 44 CN-AML patients. Among the 10 patients with NPM1 mutations, type A NPM1 mutations were identified in 8 (80%) patients, whereas non-A type NPM1 mutations were observed in 2 (20%) patients. Two non-A type NPM1 mutations were not previously reported: c.867-868InsCGGA and c.861-862InsTGCA. These two novel mutant proteins display a nuclear export signal (NES) motif (L-xxx-L-xx-V-x-L) less frequently and L-x-Lx-V-xx-V-x-L it has been never seen before, yet. However, both novel mutations show a tryptophan loss at codon 288 and 290 at the mutant C-terminus which are crucial for aberrant nuclear export of NPM into the cytoplasm. Conclusions: This study suggests previously unreported NPM1 mutations may be non-rare and thus additional sequence analysis is needed along with conventional targeted mutational analysis to detect non type-A NPM1 mutations.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients2332408944510.31557/APJCP.2021.22.1.233ENRungtiwaDangtakotBiomedical Sciences Program, Graduate School, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.Cholangiocarcinoma
Research Institute, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.0000-0001-7745-7971KittiIntuyodCholangiocarcinoma
Research Institute, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.Department of Parasitology, Faculty of Medicine,
Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.0000-0002-1096-9371AnuchaAhoojaDepartment of Radiology, Faculty of Medicine, Khon Kaen University,
Nai Mueang, Khon Kaen, Thailand.0000-0002-6279-5019JitrapornWongwiwatchaiDepartment of Radiology, Faculty of Medicine, Khon Kaen University,
Nai Mueang, Khon Kaen, Thailand.0000-0003-1338-4755PetcharakornHanpanichDepartment of Radiology, Faculty of Medicine, Khon Kaen University,
Nai Mueang, Khon Kaen, Thailand.0000-0001-9473-0319AroonlugLulitanondCenter for Research and Development of Medical Diagnostic Laboratories, Faculty of
Associated Medical Sciences, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.0000-0002-4735-5074YaovaluxChamgramolCholangiocarcinoma
Research Institute, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.Department of Pathology, Faculty of
Medicine, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.0000-0003-2349-1984SomchaiPinlaorCholangiocarcinoma
Research Institute, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.Department of Parasitology, Faculty of Medicine,
Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.0000-0002-8187-7247PorntipPinlaorCholangiocarcinoma
Research Institute, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.Center for Research and Development of Medical Diagnostic Laboratories, Faculty of
Associated Medical Sciences, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.Journal Article20201103Objective: Choledocholithiasis (CDL), a potential risk for cholangiocarcinoma (CCA) development, is often a consequence of bacterial infection. Thus, the microbial population that contributes to CDL might also be involved in CCA development. We compared the microbiome in bile fluid of CDL patients and CCA patients. Methods: Bile samples were collected from CDL (n = 30) and CCA (n =30) patients. Microbial profiling was performed individually by the sequencing of V3-V4 regions of the 16S rRNA gene. Results: Enterobacter, Pseudomonas, and Stenotrophomonas species were much more abundant in bile samples from CCA compared to CDL (pWest Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Doctor-Patient Cancer Screening Communications among Church-based Chinese Adults – The Role of Caregiver Experience and Family History2412478937710.31557/APJCP.2021.22.1.241ENSu-IHouDepartment of Health Management & Informatics, College of Community Innovation and Education, University of Central Florida,
Orlando, FL, USA.0000-0002-4519-0974Journal Article20200905Objective: Significant gap exists in the literature examining cancer screening communication related factors among Chinese immigrants. This study examined the role of cancer caregiver experience and family history on doctor-patient cancer screening communication among church-based Chinese adults. Methods: A self-administered survey was conducted among adults from 9 Chinese churches (n=372). Cancer Communication was measured by “Dr. recommended screenings” and “Talked to doctors about cancer screenings”. The survey was developed in English and translated in Chinese. Results: Mean age was 44.31 (SD=14.74), 60% were males, 72% were married, majority had college education (85%), and 17% reported had been a primary cancer caregiver and 54% reported having family cancer history. Cancer caregivers scored higher on doctor-patient cancer communication, as well as cancer knowledge and screening norms. Participants with family cancer history were also more likely to talk to doctor about screening, as well as perceived higher cancer risk, lower health status, and screening barriers. Multiple regression analyses showed that primary caregiver experience was still a significant predictor on talking to doctors about cancer screenings (OR=2.1; 95%CI=[1.10, 4.01]; p=0.025), yet doctors more like to recommend screening among caregivers became non-significant. The significant influence of family cancer history on talking with doctors on cancer screenings also disappeared. Older age (OR=2.52; p=0.006) and being married (OR=2.45; p=0.022) were significant on predicting communication of cancer screenings with doctors. Data also showed that doctors were more likely to recommend cancer screenings to older (OR=2.75, p <.001), married (OR=2.57; p=0.006) adults. Conclusion: Current study calls attentions to primary cancer caregiver experience, family history, age, and marital factors when designing tailored doctor-patient cancer screening communication programs among church-based Chinese to address cancer disparities.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101The Role of Vesicular Stomatitis Virus Matrix Protein in Autophagy in the Breast Cancer2492558944610.31557/APJCP.2021.22.1.249ENFatemeh SanaAskariStudent Research Committee, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.AlirezaMohebbiStem Cell Research
Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.0000-0003-2489-585XAbdolvahabMoradiDepartment of Microbiology, School of
Medicine, Golestan University of Medical Sciences, Gorgan, Iran.NaemeJavidDepartment of Microbiology, School of
Medicine, Golestan University of Medical Sciences, Gorgan, Iran.Journal Article20201023Background: Breast cancer is one of the most difficult malignancies to treat. Therapeutics is used to target and kill the cancer cells. Non-human oncolytic viruses have the ability to cause cell death directly to cancers. The objective here was to investigate the role of Vesicular Stomatitis Virus (VSV) Matrix (M) protein in autophagy in the breast cancer cell line. Methods: Two different VSV wild type and mutant (M51R) M protein constructs were produced. Breast cancer cell line BT-20 was transfected by either wild type or mutant vectors. Transfection efficiency was measured using a fluorescent microscopy. Expression of VSV M protein was investigated at protein level. Cell cytotoxicity was measured using an MTT assay. The autophagy pathway was studied by Beclin-1 immunoassay. Data were statistically analyzed between different transfected groups. Results: It has been shown that the VSV M protein induced higher levels of Beclin-1 than the M51R mutant in the BT-20 cell line. Increased levels of Beclin-1 were also associated with VSV M cell-induced cytotoxicity. Conclusion: It has been shown here that VSV wild type or mutant M proteins can cause autophagy-induced cell death by increasing Beclin-1 expression. This includes the possible role of VSV to be used as an oncolytic virus in breast cancer treatment. <br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822119991130As Evidence-Based Tumorigenic Role of Epstein-Barr Virus miR-BART1-3p in Neurological Tumors2572668944710.31557/APJCP.2021.22.1.257ENMohammadKarimzadehDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.AlirezaTabibzadehDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.0000-0003-0070-2208MohsenMoghoofeiDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Microbiology,
Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.SaeedehAbbasiDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.JavidSadri NahandDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.FarzinSadeghiiDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.Mohammad HadiKarbalaie NiyaGastrointestinal and Liver Disease Research
Center (GILDRC), Iran University of Medical Sciences, Tehran, Iran.HossinKeyvaniDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.FarahBokharaei-SalimDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.Laboratory of National Center, Vice Chancellor for Health, Iran University of Medical Sciences, Tehran, Iran.MoeinEsghaeiCognitive Neuroscience Laboratory, German Primate Center - Leibniz Institute for Primate Research, Göttingen, Germany.MaryamEsghaeiDepartment of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.0000-0003-2944-2147Journal Article19991130Introduction: Central nervous system tumors are a diverse group of tumors that account for 2% of all adult cancers and 17% of childhood malignancies. Several internal and external risk factors are involved in the development of this cancer such as viral infections. The aim of this study was to the determination of the EBV infection frequency and the expression level of miR-122 and miR-BART in CNS tumors samples. Methods: One hundred and thirty-eight fresh tissue sample (106 case and 32 control) was collected from CNS specimens. The presence of Epstein-Barr virus (EBV) DNA was examined by PCR assay and the expression level of miR-122 and miR-BART were evaluated by using real-time PCR assay in CNS tissue samples. Results: EBV DNA was detected in 17% (18 of 106) of tumors tissue samples and 6.4% (2 of 32) of control samples. according to results, there was a significant relationship between the presence of EBV-DNA with CNS tumors. Additionally, the expression level of miR-122 was significantly downregulated in the EBV-positive sample compared to that of the EBV-negative sample. Also, the level of EBV-BART1-3p expression was significantly higher in EBV-positive tumors samples than EBV-positive normal samples. Conclusion: The results of this study suggest that the EBV could change the condition of cancer cells by altering the expression of miR-122 and EBV-BART1-3p and maybe contribute to the development of cancer cells. However, the role of viral infections in CNS cancer requires further studies. <br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Janus Kinase-2 Mutation Associated Portal Vein Thrombosis Complicating Liver Cirrhosis and Hepatocellular Carcinoma2672758944810.31557/APJCP.2021.22.1.267ENHatemRabieDepartments of Clinical Pathology, National Liver Institute, Menoufia University, Egypt.WardaOthmanHepatology, National Liver
Institute-Menoufia University, Egypt.0000-0001-9651-0416Dalia MElsabaawyLecturer of Clinical Pharmacy, National Liver Institute, Menoufia University, Egypt.Eman E.ElshemyHepatogastroentrology and Infectious Diseases, Faculty of Medicine for girls, AL-Azhar University, Egypt.NeamatAbdelmageedHepatogastroentrology and Infectious Diseases, Faculty of Medicine for girls, AL-Azhar University, Egypt.Fatma A.KhalafDepartment of
Biochemistry , National Liver Institute, Menoufia University, Menoufia, Egypt.Hanan MBedairDepartments of Clinical Pathology, National Liver Institute, Menoufia University, Egypt.Journal Article20200720Background: Portal vein thrombosis (PVT) might be a catastrophic event complicating liver cirrhosis and hepatocellular carcinoma (HCC). Aim: role of JAK2 RS V617F mutation as a risk factor for PVT development in liver cirrhosis and HCC. Methods: A case control study conducted on 100 PVT patients (76 HCC and 24 liver cirrhosis) additionally, 100 healthy individuals used as a control group. PVT was diagnosed incidentally by Doppler ultrasound during routine follow-up HCC screening. Prothrombin G20210A mutation, MTHFR mutation, Factor V Leiden mutation (VFL), antithrombin III (ATIII), protein C, S, and antiphospholipid antibodies, along with JAK2 RS V617F mutation by real-time polymerase chain reaction all were analyzed. Results: Patients with PVT were significantly older (p <0.001), thrombocytopenic (p <0.001), with high alkaline phosphatase (p <0.001). JAK2 RS V617F mutation was found in 28/100 (28%) in idiopathic PVT complicating liver cirrhosis and hepatocellular carcinoma. Cases with positive JAK2 rs V617F mutation were significantly accompanied by protein S deficiency (P 0.03), LA absence (p 0.06), and high frequency of ascites (P 0.03). While, the MTHFR heterozygous mutation (p0.001), ATIII (P 0.02), and VFL (P 0.01) were more frequent with negative JAK2 rs V617F mutation. The comparison between demographic data and thrombophilic parameters in PVT cases revealed that no significant differences were recorded except for male gender, Diabetes Mellitus, splenomegaly significantly increased among HCC cases (p <0.05). Conclusions: JAK2 rs V617F mutation must be considered in any case of PVT with liver cirrhosis and hepatocellular carcinoma without identified thrombophilic risk factors, with potential considerations of evolving myeloproliferative disorders. New diagnostic and therapeutic implications are still awaited.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101A Cross-Sectional Study about Knowledge, Awareness and Perception of Risk Factors for Cancer among Cancer-Patients Relatives and Healthy Adults in Ho Chi Minh City, Vietnam2772858944910.31557/APJCP.2021.22.1.277ENDung XPhamHo Chi Minh City Oncology Hospital, Vietnam.Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam.Tien T TPhamDepartment of Psychology, Hoa sen University, Ho Chi Minh City, Vietnam.Thang NPhamDepartment of Psychology, Hoa sen University, Ho Chi Minh City, Vietnam.Tung DBuiHo Chi Minh City Oncology Hospital, Vietnam.0000-0002-0431-8820Journal Article20200415Background and Aims: Although cancer is common in Ho Chi Minh city, Vietnam, the community awareness is still unknown. The primary objective of this study was to examine and compare the knowledge and risk perceptions of cancer possessed by cancer patients - relatives and healthy adults in Ho Chi Minh City, Vietnam. Methods: A cross-sectional study was conducted from June to August 2019. Cancer patients and their relatives were drawn from those who were hospitalized in the Oncology Hospital, Ho Chi Minh City. Healthy individuals were those without a known diagnosis of cancer, and they were drawn from the participants of the Vietnam Osteoporosis Study. A total of 533 participants including 249 patients and relatives (cancerous group) and 284 healthy individuals (healthy group), were asked to respond to a structured questionnaire that was comprised of items concerning cancer knowledge, risk factor perception, and general attitude towards cancer, using Yes, No, or Likert Scale for response. Results: The findings showed that patients hold poorer knowledge of pathology, signs, symptoms, prevention, and treatment and lower awareness of risk factors but more positive attitude towards cancer as compared to their healthy counterparts. Overall, both groups varied in their cancer knowledge, with many areas remain to be improved. Conclusions: Knowledge about cancer and its risk factors should be improved among the general population as well as among those with direct experiences with cancer. Practical implications: The findings provided by this study has major implications for the design of an educational program for cancer patients in clinical settings and awareness programs for the general public as a primary preventive measure for mitigating the cancer burden. Future studies with larger and more diverse samples or qualitative studies exploring the personal narratives of people living with cancer could take advantage from the preliminary data provided by this study. <br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Relationship between Personal Values, Work Experience and Nursing Competencies among Cancer Care Nurses in Malaysia2872948945010.31557/APJCP.2021.22.1.287ENNor AidaMaskorProfessional Development and Continuing Education, Faculty of Educational Studies, Universiti Putra Malaysia, Malaysia.MazanahMuhamadProfessional Development and Continuing Education, Faculty of Educational Studies, Universiti Putra Malaysia, Malaysia.StevenEric KraussProfessional Development and Continuing Education, Faculty of Educational Studies, Universiti Putra Malaysia, Malaysia.Nik HasnaaNik MahmoodRazak
Faculty of Technology and Informatics, Universiti Teknologi Malaysia, Malaysia.Journal Article20180301Oncology nurses are an essential component of cancer care teams. Nurses play a vital role in ensuring that cancer patients comply with their cancer treatment. In the cancer care nursing context, competency is not merely being skilled, but also implies the characteristic of being able to perform effectively. In addition to the need for competence, nursing is a discipline rich in values including human dignity, caring, humanity, and respect for personal privacy. Research from a variety of disciplines indicates that values often influence human behaviour in professional and work settings. It is often believed, therefore, that nurse’ values and work experience influence and contribute to their work performance. Few studies have attempted to examine these relationships, particularly in the context of cancer care nursing. The purpose of this study was to determine the relationship between personal values, work experience and competency among cancer care nurses in Malaysia. Quantitative surveys were used to collect the data. A total of 845 cancer care nurses from 38 public hospitals in Peninsular Malaysia participated in the study. Descriptive statistics and Pearson Product-Moment Correlations were used to analyse the data. The findings revealed positive and significant relationships between personal values and competency (r = 0.59, p < 0.01) and work experience and competency (r = 0.11, p < 0.047). The findings support the assertion that Malaysian nurses’ values and work experience are related to performance-related competency.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Experiences of End-of-Life Care of Older Adults with Cancer From the Perspective of Stakeholdersin Iran: A Content Analysis Study2953008945110.31557/APJCP.2021.22.1.295ENZohrehGhezelsefliDepartment of Nursing, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.FazlollahAhmadiDepartment of Nursing, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.EesaMohammadiDepartment of Nursing, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.MartinePuts RNDepartment of Nursing, Lawrence S.
Bloomberg Faculty of Nursing, University of Toronto , Toronto, Ontario, Canada.Journal Article20190827Objectives:To describe end-of-life care forolder adults with cancer admitted to the hospital in Tehran, Iranto determine if there were any gaps in care for older adultsthat can be improved. Materials:This study used a qualitative descriptive study design. In total, 37 individualsincluding patients, healthcare team members, and family caregivers, participated in the study. Semi-structured interviews using topic guides were conducted, and the thematic content analysis method described by Braun and Clarke (2006), was used to analyze the data. Results: In total, 37 Iranian participants (12 male and 25 female), including 14 nurses, 3 oncologists, 1 social worker, 1 chaplain, 1 psychologist, 11 family members and 6 patientsinterviewed.Our main themes of end-of-life carewere:1) barriers to providing and receiving quality care for families and patients; and 2) coping strategies and empowerment of families and patients. Conclusion: Healthcare providers are recommended to familiarize themselves with the burden faced by patients and family caregivers who take care of older adults with chronic diseases at home, and they should organize their supportive and consulting actions. In order to improve the quality of life of older patientsand their family caregivers.West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Randomized Study of enterade® to Reduce Diarrhea in Patients Receiving High-Dose Chemotherapy and Autologous Hematopoietic Stem Cell Transplantation3013048945210.31557/APJCP.2021.22.1.301ENZachariahDe FilippBlood and Marrow Transplant Program, Massachusetts General Hospital, Boston, MA, USA.BrettGlotzbeckerDepartment of Clinical Oncology,
Dana Farber Cancer Institute, Boston, MA. USA.LauraLuqueDepartment of Science & Technology, Entrinsic Bioscience, Norwood, MA, USA.Haesook TKimDepartment of Clinical Research, Dana Farber Cancer Institute, Boston, MA, USA.Katherine MMitchellDepartment of Science & Technology, Entrinsic Bioscience, Norwood, MA, USA.Samuel N.CheuvrontDepartment of Science & Technology, Entrinsic Bioscience, Norwood, MA, USA.Robert JSoifferDepartment of Clinical Oncology, Dana Farber Cancer Institute, Boston, MA. USA.Journal Article20200825High-dose chemotherapy frequently causes injury to the gastrointestinal mucosa, resulting in diarrhea. The purpose of the current study was to assess the tolerability and efficacy of enterade® in reducing ≥ grade 2 diarrhea (G2D) in association with high-dose melphalan followed by autologous stem cell transplantation (ASCT). We conducted a prospective, double blinded, multi-center trial in which 114 subjects were randomized to receive enterade® or placebo twice daily during the transplant hospitalization. Gastrointestinal toxicities (nausea, vomiting, oral mucositis and dysphagia) resulted in poor study compliance in both arms. Among subjects who were able to complete planned therapy (13%), the incidence of G2D was lower for those receiving enterade® as compared to placebo (16% vs 86%, p <0.03). Twice daily oral administration of enterade® and placebo following high-dose chemotherapy and ASCT was not feasible due to significant gastrointestinal toxicities. Future explorations of enterade® should be conducted in populations capable of reasonable oral intake.<br /> West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.Asian Pacific Journal of Cancer Prevention1513-736822120210101Let-7d and miR-185 Impede Epithelial-Mesenchymal Transition by Downregulating Rab25 in Breast Cancer3053138945310.31557/APJCP.2021.22.1.305ENArmanShahabiInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Molecular
Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.BehroozNaghiliInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.KhalilAnsarinTuberculosis and Lung
Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.MaryamMontazeriDepartment of Medical Biotechnology, Faculty of
Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.MehdiDadashpourStem Cell Research Center,
Tabriz University of Medical Sciences, Tabriz, Iran.0000-0003-3352-7880NosratollahZarghamiInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Molecular
Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.Journal Article20200921Objective: MicroRNAs (miRNAs) expression has deregulated in several cancer types including breast cancer (BC). The present study aims at investigating the role, mechanism, clinical value of let-7d and miR-185 in BC, and the possible correlation these miRNAs with Rab25. Materials and Methods: Tumor samples as well adjacent normal tissues (ANT) were acquired from fresh surgical specimens from 110 patients and the expression levels of let-7d, miR-185, Rab25, and snail were evaluated using real-time PCR. The immunohistochemical (IHC) process and western blot were done to detect the level of Rab25 and Snail protein expression in BC samples. Results: By comparing miRNAs expression profiles in clinical tissues of 110 patients using real-time PCR, let-7d, and miR-185 expression were dramatically downregulated in BC tissues (P < 0.05). Tumor size, stage, and lymph node metastasis were significantly related to miRNAs expression. Based on qRT-PCR and bioinformatics database analyses, we also recognized Rab25 as a possible target of miR-185 and let-7d. Rab25 expression was enhanced in BC cells and associated inversely with the expression level of mentioned miRNAs. qRT-PCR, immunohistochemistry, and western blot studies verified that Rab25 upregulation increased the levels of the snail, that key transcription factor of epithelial-mesenchymal transition (EMT). Conclusion: These findings demonstrated that let-7d and miR-185 inhibited EMT by targeting Rab25 expression in BC. Therefore, targeting the let-7d and miR-185/Rab25 interaction may offer new therapeutic opportunities for treating BC patients.<br />