Microglial Contribution to Glioma Progression: an Immunohistochemical Study in Eastern India

Document Type: Systematic Review and Meta-analysis

Authors

1 Immunobiology Lab, Department of Zoology, Panihati Mahavidyalaya, Barasat Road, Sodepur, Kolkata, West Bengal, India

2 Immunobiology Lab, Department of Zoology, Panihati Mahavidyalaya, Sodepur, Kolkata, West Bengal, India

3 Department of Neurosurgery, Bangur Institute of Neurosciences, Kolkata, West Bengal, India

4 Department of Pathology, SSKM Hospital, IPGME&R, Kolkata, West Bengal, India

5 Department of Experimental Medicine, School of Tropical Medicine, Kolkata, West Bengal, India

Abstract

Human glioma, arising from glial cells of the central nervous system, accounts for almost 30%of all brain tumours , neoplasms with a poor prognosis and high mortality rates worldwide. In the present study we assessed tissue architectural modifications associated with macrophage lineage cells, controversial major immune effector cells within the brain, in human glioma tissue samples from eastern India. Ethically cleared post-operative human glioma samples from our collaborative neurosurgery unit with respective CT/MRI and patient history were collected from the Nodal Centre of Neurosciences in Kolkata, over 9 months. Along with conventional histopathology, samples were subjected to silver-gold staining and fluorescence tagged immunophenotyping for the detection of electron dense brain macrophage/microglia cells in glioma tissue, followed by immune-phenotyping of cells. With higher grades, CD11b+/Iba-1+ macrophage/microglia architecture with de-structured boundaries of glioma lesions indicated malfunction and invasive effector state. Present study documented a contribution of microglia to glioma progression in Eastern India.

Keywords