The aim of this study was to determine the incidence and predictors of overtreatment in “see and treat”approach using loop electrosurgical excision procedure (LEEP) in women with high-grade squamousintraepithelial lesion (HSIL) on cervical cytology. The overtreatment was considered when LEEP specimenscontained no cervical pathology. Between January 2001 and April 2006, 446 women with HSIL on Pap smearunderwent colposcopy followed by LEEP at Chiang Mai University Hospital. Mean age of these patients was45.6 years with a range of 25-78 years. One hundred and twenty-one (27.1%) women were menopausal.Unsatisfactory colposcopy was observed in 357 (80.0%) women. Of 446 women, histologically-confirmed HSIL,invasive cancer, low-grade squamous intraepithelial lesions, and adenocarcinoma in situ were detected in 330(74.0%), 76 (17.0%), 9 (2.0%), and 5 (1.1%), respectively. The overtreatment rate on LEEP specimens wasnoted in 26 women or 5.8% (95% confidence interval [CI] = 3.8 to 8.4) of 446 women. By multivariate analysis,postmenopausal status was the only significant independent predictor of overtreatment with an adjusted oddsratio of 2.89 (95% CI = 1.30 to 6.43, P = 0.009). When postmenopausal women were excluded from analysis, theovertreatment rate was reduced to only 4.0%. In conclusion, “see and treat” approach appears to be anappropriate strategy in managing women with HSIL cytology. The overtreatment rate could be reduced whensuch policy is limited for premenopausal women.