Oral mucositis is an important and common complication among female patients who have cancer of thereproductive system receiving chemotherapy. This study aimed to evaluate the prevention of chemotherapyinducedoral mucositis project in female reproductive system cancer patients at Maharaj Nakorn Chiang MaiHospital. The clinical practice guidelines evaluation model of the Registered Nurses Association of Ontario(RNAO, 2002) was used as a framework. The subjects included 14 nurses and practical nurses, and 404 patientreports. Data were collected by using of two forms developed by the researcher; the nurses’ opinion form aboutthe project’s implementation and a mucositis form. Data analysis was conducted using frequency, percentage,and mean. The findings showed that 92.9 % of the subjects reported that they could consistently follow theclinical practice guidelines. All of them (100.00 %) agreed that the clinical practice guidelines were easily tofollow, convenient to use, had good outcome, reduced nursing time, and were satisfied with this project. Afterthe project’s implementation, it was found that mucositis was reduced from 22.0 % to 9.9 %. The results of thisstudy confirm that with the prevention chemotherapy-induced oral mucositis project for female reproductivesystem cancer patients, care is more efficient. These results could be extended for use in other similar settings.