Persistent high-risk human papillomavirus (HPV) infection is known to play an important role in thegenesis of cervical cancer. Since new screening and prevention strategies, namely improved HPV testing andHPV vaccination have been aggressively promoted recently, it is crucial to investigate the HPV distribution inMalaysia in order to maximize their cost-effectiveness. This study was therefore conducted to assess the HPVtype distribution in the most populous region, the state of Selangor. A total of 200 cervical swab samples werecollected in two health-screening campaigns, and also from women attending obstetrics and gynecology clinics inseveral hospitals in Selangor. DNA extraction was performed and HPV DNA was detected via nested PCR usingMY09/MY11 as outer primers and GP5+/GP6+ as inner primers which target the L1 gene of the viral genome.The purified PCR products were subjected to automated DNA sequencing to determine the HPV genotype. Outof 180 β-globin positive samples, 84 (46.7%) were positive for HPV DNA. The most common HPV type foundwas high-risk oncogenic type 16 (40%), followed by HPV type 18 (3.3%), HPV 33 (1.7%), HPV 31 (0.6%), andlow-risk HPV 87 (0.6%). Our study confirmed that nested PCR method is highly sensitive in detecting HPV DNAeven in low risk patients. Since a relatively high prevalence rate of HPV infection was found in this population,prompt healthcare policy changes to bring about implementation of early HPV vaccination program is desirableto prevent a high incidence of cervical cancer.