Changes of Androgen Receptor and Insulin-Like Growth Factor-1 in LNCaP Prostate Cancer Cells Treated with Sex Hormones and Flutamide


Changes of androgen receptor (AR) and insulin-like growth factor-1 (IGF-1) were investigated in LNCaPcells treated with 5α-dihydrotestosterone (DHT), estrone and flutamide. Real-time PCR, immunocytochemistryand western blotting were used to detect the expression of AR and IGF-1 in the presence or absence of variouskinase inhibitors. Low concentrations of DHT, estrone and flutamide increased the expression of AR and IGF-1,especially estrone, with concentration and time dependence. With DHT and flutamide, there was a significantalteration in AR expression (p<0.001). The results indicated expression of AR and IGF-1 genes to be influencedby DHT, estrone and flutamide in LNCaP cells, regulated by multiple signal pathways.