Breast cancer is the most common malignancy in women in the world. High incidence and poor clinicaloutcomes underly the need for a better understanding of its tumor biology and how to effectively inhibittumor progression. In the present study the question of whether NDRG2 might be a useful target for breastcancer therapy was addressed. With the increase or decrease of NDRG2 levels in MCF-7 and Bcap-37 cellsby adenovirus-NDRG2 infection or NDRG2 siRNA transfection, CD24 expression was significantly decreasedor increased, respectively. Furthermore, NDRG2 overexpression suppressed breast cancer cell adhesion andinvasion, whereas knockdown of NDRG2 promoted these events. In conclusion, the data from the current studyindicated that NDRG2, the product of a tumor suppressor gene, can regulate CD24 expression to decrease themetastatic potential of breast cancer cells.