Benign prostate hyperplasia (BPH) is the most common benign tumor in elderly men for which the HPC2/ELAC2 and SRD5A2 genes are known genetic factors. The HPC2/ELAC2 gene features Ser217Leu andAla541Thr polymorphisms and the SRD5A2 gene Ala49Thr and Val89Leu polymorphisms. The aim of this studywas to examine relationships between these polymorphisms and BPH in Turkish men using amplification bythe polymerase chain reaction (PCR) method. Polymorphisms were determined by using restriction fragmentlength polymorphism (RFLP) with suitable restriction: TaqIα, Fnu4HI, Mwo I and Rsa I. We found statisticallysignificant relationship between the SRD5A2 gene Ala49Thr (OR=2.3; CI 95%, 1.04-5.1; p=0.01<0.05) , but notthe other polymorphisms, and BPH. For the first time, our data demonstrate that correlation between SRD5A2gene Ala49Thr and polymorphism are statistically significant in Turkish men with BPH.