MiR-138 Suppresses Expression of Hypoxia-inducible factor 1α (HIF-1α) in Clear Cell Renal Cell Carcinoma 786-O Cells


Objectives: Hypoxia-inducible factor-1alpha (HIF-1a) is widely considered to be one of the key regulatorsin cancer cells. Here, we investigated a microRNA regulating expression of HIF-1a and explored its functionsin clear cell renal cell carcinoma (ccRCC) cells. Methods and materials:Western blot and reporter assays wereused to assess HIF-1a as a direct target of miR-138. The effects of miR-138 or si-HIF-1a on ccRCC 786-O cellswere also estimated by apoptosis analysis and cell migration assay.
Results: The data showed HIF-1a to be onetarget of miR-138. Futhermore, inhibition of the expression of HIF-1a with specific siRNA or miR-138 couldincrease apoptosis and reduce the migration of 786-O cells.
Conclusions: miR-138 could inhibit the expressionof HIF-1a and regulate the apoptosis and migration of ccRCC cells.