Antimutagenicity and Antioxidative DNA Damage Properties of Oligomeric Proanthocyanidins from Thai Grape Seeds in TK6 Cells

Oligomeric proanthocyanidins (OPCs) are found mostly in red grape seeds. Many publications have reportedthat OPCs possess an excellent anti-oxidant effects. Since it could against cellular damage from reactive oxygenspecies (ROS) led to reduce the risk of chronic disease and cancers. We carried out this study on the ThaiOPCs to evaluate the mutagenicity/ anti-mutagenicity and anti-oxidative DNA damage effects in TK6 cells bymicronucleus (MN) and comet assays. In the MN assay, OPCs-treatment of TK6 cells at concentrations rangingfrom 10-200 μg/ml (4 and 24 h) did not cause micronucleus induction over the negative control group but revealeda significant reduction the micronucleus frequencies against the known mutagen (mitomycin C). In the cometassay, OPCs-treated TK6 cells at concentrations of 100, 250, 500, and 1,000 μg/ml could inhibit DNA damageinduced by H2O2 as indicated by 18.7, 36.4, 30.6, and 60.1%, respectively. Our results suggest that OPCs possessthe anti-mutagenic and anti-oxidative DNA damage effects in TK6 cells under the conditions of this assay.