Cervical cancer is the third most common cancer in women in peninsular Malaysia and very prevalentworldwide. HPV vaccination and routine Pap smear testing are the best preventive measures. The objectiveof this study was to determine the knowledge level of secondary school students from Sarawak, East Malaysiaregarding cervical cancer and its prevention. Multistage random sampling with various methods in each step wasemployed to select the sample of 76 students. Results showed that 61.8 % had poor knowledge level of cervicalcancer and its prevention. There were 60.5 % of students who were aware of cervical cancer with Chinese andform four students showing significantly the highest awareness (p< 0.05). The main source of cervical cancerinformation was from their parents (25.9 %). HPV vaccination acceptance among students was 22.3 % and anassociation was found between knowledge of cervical cancer with race and HPV vaccination acceptance (p<0.05). In conclusion, the students had poor knowledge level of cervical cancer, its prevention and HPV vaccinationacceptance. More efforts should be made to improve cervical cancer knowledge and awareness of the publicespecially secondary school students in Sarawak. This in turn will enhance the practice of prevention againstcervical cancer among students.