Neural precursor cell-expressed developmentally down-regulated 8 (NEDD8), a ubiquitin-like protein,mainly functions through covalent ligation to cullin proteins. Conjugation of NEDD8 with cullins can promoteubiquitination, which plays a critical role in the degradation of many proteins. UBA3 is the subunit of NEDD8-activating enzyme which is one of the keys for NEDD8 linkage to cullin proteins. Previous research showedNEDD8 conjugation to be up-regulated in highly proliferative cell lines. In the present study, up-regulated NEDD8conjugation was observed in melanoma cell lines by Western blot analysis. After down-regulation with a RNAi toUBA3, proliferation of M14 was suppressed in vitro and in vivo. In conclusion, up-regulated NEDD8 conjugationmay be involved in the development of melanoma. Interference in this pathway might offera promising methodfor melanoma therapy.
(2012). Inhibition of the NEDD8 Conjugation Pathway by shRNA to UBA3, the Subunit of the NEDD8-Activating Enzyme, Suppresses the Growth of Melanoma Cells. Asian Pacific Journal of Cancer Prevention, 13(1), 57-62.
MLA
. "Inhibition of the NEDD8 Conjugation Pathway by shRNA to UBA3, the Subunit of the NEDD8-Activating Enzyme, Suppresses the Growth of Melanoma Cells". Asian Pacific Journal of Cancer Prevention, 13, 1, 2012, 57-62.
HARVARD
(2012). 'Inhibition of the NEDD8 Conjugation Pathway by shRNA to UBA3, the Subunit of the NEDD8-Activating Enzyme, Suppresses the Growth of Melanoma Cells', Asian Pacific Journal of Cancer Prevention, 13(1), pp. 57-62.
VANCOUVER
Inhibition of the NEDD8 Conjugation Pathway by shRNA to UBA3, the Subunit of the NEDD8-Activating Enzyme, Suppresses the Growth of Melanoma Cells. Asian Pacific Journal of Cancer Prevention, 2012; 13(1): 57-62.
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