PBK/TOPK Expression During TPA-Induced HL-60 Leukemic Cell Differentiation


Objective: This study concerns expression of PBK/TOPK during differentiation of HL-60 leukemic cellsinduced by tetradecanoyl phorbol acetate (TPA).
Methods: Wright-Giemsa staining was performed to observemorphological changes in the HL-60 cells, and flow cytometry was used to assess the cell cycle and CD11b,CD14, CD13, and CD33 expression. PBK/TOPK levels were determined by Western blot analysis.
Results: Aftertreating HL60 cells with 5.1 × 10-9 mmol/L of TPA for three days, the number of nitroblue-tetrazolium-positivecells and CD11b, CD13, and CD14 expression increased, whereas the PBK/TOPK levels decreased.
Conclusions:TPA can inhibit proliferation and induce differentiation of HL60 cells of the granulocytic or monocytic lineage.PBK/TOPK expression was downregulated during this process, whereas the Pho-PBK/TOPK expression wasincreased.