Effects of Monoclonal Antibodies against Human Stathmin Combined with Paclitaxel on Proliferation of the QG-56 Human Lung Carcinoma Cell Line


Objective: To explore whether monoclonal antibodies against stathmin and the chemotherapuetic agentpaclitaxel have synergenic effects in inhibiting growth and inducing apoptosis in human QG-56 cells.
Methods:QG-56 cells were treated with monoclonal antibodies against stathmin or paclitaxel alone or in combination,with untreated cells used as controls. After 24, 48, 72 and 96 hours the cell growth condition was observed underan inverted microscope and inhibition was studied by MTT assay; apoptosis was analyzed by flow cytometry.
Results: The populations decreased and cell shape and size changed after the various treatments. Monoclonalantibodies against stathmin and paclitaxel used alone or incombination inhibited the proliferation of QG-56cells, especially in combination with synergism (P<0.05). Combined treatment also resulted in a significantlyhigher apoptosis rate than in the other groups (P<0.05).
Conclusions: Monoclonal antibodies against stathminand paclitaxel used alone or in combination can inhibit proliferation of QG-56 cells and induce apoptosis whenapplied together, The observed synergistic effects may have important implications for clinical application.