Objective: To explore serum angiogenic factor expression in patients with osteosarcoma and its relationshipwith metastasis.
Methods: Immunohistochemistry was used to test the expression of CD34 and FⅧ-Rag inosteosarcoma tissues of 36 patients (osteosarcoma group) and microvessel density (MVD) was also recorded.In addition, ELISA was used to test the level of vascular endothelial growth factor (VEGF), basic fibroblastgrowth factor (bFGF), transforming growth factor-β1 (TGF-β1) and endostatin (ES) in the osteosarcoma groupand in a control group.
Results: VEGF and ES level were significantly higher than in the control group beforeoperation (P<0.01), VEGF, bFGF and TGF-β1 correlating with the ES level (P<0.01). Serum VEGF and ESlevels of osteosarcoma patients before surgery were closely related to relapse and metastasis; moreover, serumVEGF increased with MVD (P<0.01). Postoperative VEGF and ES levels were lower than the preoperationvalues (P<0.01); ES level in relapse group was significantly higher than that of the non-relapse group (P<0.01).
Conclusion: Preoperative serum VEGF and postoperative ES levels have great predictive value with regard torelapse of osteosarcoma patients.