Our aim was to test the hypothesis that preoperative serum levels of matrix metalloproteinase-7 (MMP-7)and -9 (MMP-9) and tissue inhibitor of matrix metalloproteinase (TIMP-1) levels correlate with pathologicalfeatures. Serum levels of MMP-7, and MMP-9 and TIMP-1 were determined in 90 bladder cancer patients and40 healthy controls using an enzyme linked immunosorbent assay. Preoperative serum MMP-7 and MMP-9levels were significantly higher in cancer patients than control groups (p<0.001). In contast, serum TIMP-1 levelswere lower (p<0.001). Alteration in MMP-7, and MMP-9, and TIMP-1 production may contribute to tumorangiogenesis and be associated with clinic-pathological features.