Cervical cancer is the most important female gynecological cancer, the second leading cause of cancer mortalityin women worldwide and the second most common cancer in Thai women. The major cause of cervical cancer ispersistent infection of human papillomavirus (HPV), leading to abnormal epithelial lesions, with progression toprecancerous and invasive cancer. This study was conducted to investigate the frequency and type distributionof HPV in Thai women who had abnormal cytology. HPV detection from FFPE confirmed abnormal of highgrade cervical intraepithelial lesions were for SPF-10-Innogenic Line Probe Assay. HPV-positivity was detectedin 320/355 cases (90.14%) and HPV-negativity in 35/355 (9.86%). HPV-positive was found 147/320 cases (41.4%)of single infection, whereas 173/320 cases (48.7%) showed the multiple HPV infection. The most common seventypes were HPV-16, -52, -18, -11, -51, -31 and -33, in that order. HPV 16 and 18, the important oncogenic HPVtype, were observed in 64.8% of HSIL cases. Interestingly, a high proportion of multiple infections was found inthis study and more than ten types could be detected in one case. Therefore, HPV infection screening programin women is essential, particularly in Thailand. Effective primary and secondary prevention campaigns thatreinforce HPV screening for HPV detection and typing may be decrease the incidence and mortality of cervicalcancer in the future and may lead to significantly improve the quality of life in Thai women.