In the present study, investigations were carried out to screen the anticancer activities of fenugreek seedoil against cancer cell lines (HEp-2, MCF-7, WISH cells), and a normal cell line (Vero cells). Cytotoxicity wasassessed with MTT and NRU assays, and cellular morphological alterations were studied using phase contrastlight microscopy. All cells were exposed toi 10-1000 μg/ml of fenugreek seed oil for 24 h. The results show thatfenugreek seed oil significantly reduced the cell viability, and altered the cellular morphology in a dose dependentmanner. Among the cell lines, HEp-2 cells showed the highest decrease in cell viability, followed by MCF-7, WISH,and Vero cells by MTT and NRU assays. Cell viability at 1000 μg/ml was recorded as 55% in HEp-2 cells, 67%in MCF-7 cells, 75% in WISH cells, and 86% in Vero cells. The present study provides preliminary screeningdata for fenugreek seed oil pointing to potent cytotoxicity against cancer cells.