Anti-inflammatory and Anticancer Activities of Ethanol Extract of Pendulous Monkshood Root in vitro


Aim: Pendulous monkshood root is traditionally used for the treatment of several inflammatory pathologiessuch as rheumatisms, wounds, pain and tumors in China. In this study, the anti-inflammatory and anticanceractivities and the mechanism of crude ethanol extract of pendulous monkshood root (EPMR) were evaluatedand investigated in vitro. Materials and
Methods: The cytotoxic effects of EPMR on different tumor cell lineswere determined by the MTT method. Cell apoptosis and cell nucleus morphology were assessed by Hoechst33258 staining. Moreover, nitric oxide (NO) levels and intracellular oxidative stress in peritoneal macrophageswere determined to further elucidate mechanisms of action.
Results: The data showed that EPMR could producesignificant dose-dependent toxicity on three kinds of tumor cells. Furthermore, EPMR displayed obvious antiinflammatoryeffects on LPS-induced mouse peritoneal macrophages at the dosage of 4 - 200 μg/mL. The resultsdemonstrated the therapeutic potential of Pendulous Monkshood Root on cancer and inflammatory diseases.
Conclusion: Our results indicate that EPMR has anti-inflammatory and anticancer properties, suggesting thatpendulous monkshood root may be a useful anti-tumor and anti-inflammatory reagent in the clinic.