MicroRNA-451 Inhibits Growth of Human Colorectal Carcinoma Cells via Downregulation of Pi3k/Akt Pathway

Abstract

MicroRNAs (MiRNAs) play important roles in coordinating a variety of cellular processes and abnormalexpression has been linked to the occurrence of several cancers. The miRNA miR-451 is downregulated incolorectal carcinoma (CRC) cells, suggested by several research groups including our own. In this study, syntheticmiR-451 mimics were transfected into the SW620 human CRC cell line using Lipofectamine 2000 and expressionof miR-451 was analyzed by real time PCR, while expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2was analyzed by Western blot, and cell growth was detected by MTT assay. In comparison to the controls, asignificant increase in the expression of miR-451 was associated with significantly decreased expression of CAB39,LKB1, AMPK, AKT, PI3K and Bcl2. The capacity of cell proliferation was significantly decreased by miR-451expression, which also inhibited cell growth. Our study confirmed that miR-451 has a repressive role in CRCcells by inhibiting cell growth through down-regulating the P13K/AKT pathway.

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