Lack of KRAS Gene Mutations in Chronic Myeloid Leukemia in Iran


Background: The single most common proto-oncogene change in human neoplasms is a point mutation in RASgenes. A wide range of variation in frequency of KRAS mutations has been seen in hematologic malignancies.Despite this, RAS roles in leukemogenesis remain unclear. The frequency of KRAS mutations in CML has beenreported to be between zero an 10%. Many attempts have been done to develop an anti-RAS drug as a therapeutictarget. . Materials and
Methods: This cross sectional study was performed in Mashhad University of MedicalSciences, Mashhad, Iran from 2010-2012. In 78 CML patients (diagnosed according to WHO 2008 criteria)in chronic or accelerated phases, KRAS mutations in codons 12 and 13 were analyzed using a modified PCRrestrictionfragment length polymorphism (RFLP) method.
Results: We did not detect any KRAS mutations inthis study.
Conclusions: KRAS mutations are overall rare in early phase CML and might be secondary eventshappening late in leukemogenesis cooperating with initial genetic lesions.