Mechanisms of Hela Cell Apoptosis Induced by Abnormal Savda Munziq Total Phenolics Combined with Chemotherapeutic Agents


Objective: To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined withcisplatin and docetaxel on the Hela cell growth.
Methods: In vivo cultured Hela cells were treated with cisplatin,docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assessinhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test forsurvivin and Bcl-2 expression.
Results: The total phenolics, cisplatin and docetaxel had significant inhibitory andapoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of 12.8±0.70%, 18.9±3.79%and 15.8±3.8)%; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 andsurvivin (all P<0.01), especially when used in combination.
Conclusion: ASMq total phenolics, combined withcisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression ofBcl-2 and survivin.