Momordica cochinchinensis Seed Extracts Suppress Migration and Invasion of Human Breast Cancer ZR-75-30 Cells Via Down-regulating MMP-2 and MMP-9

Objective: Metastases and invasion are the main reasons for oncotherapy failure. Momordica cochinchinensis(Mu Bie Zi in Chinese) had been used for a variety of purposes, and shown anti-cancer action. In this article, wefocused on effects on regulation of breast cancer cell ZR-75-30 metastases and invasion by extracts of Momordicacochinchinensis seeds (ESMCs).
Methods: Effect of ESMCs on ZR-75-30 human breast cancer cells proliferationwere evaluated by MTT assay and on invasion and migration by wound-healing and matrigel invasion chamberassays. Expression and protease activity of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, wereanalyzed by Western blotting and gelatin zymography, respectively.
Results: ESMC revealed strong growthinhibitory effects on ZR-75-30 cells, and effectively inhibited ZR-75-30 cell invasion in a dose-dependent manner.Western blot and gelatin zymography analysis showed that ESMC significantly inhibited the expression andsecretion of MMP-2 and MMP-9 in ZR-75-30 cells.
Conclusions: ESMC has the potential to suppress the migrationand invasion of ZR-75-30 cancer cells, and it might prove to of interest in the development of novel inhibitorsfor breast cancer.