Association of Benign Prostate Hyperplasia with Polymorphisms in VDR, CYP17, and SRD5A2 Genes among Lebanese Men


Background: The aim of the study was to investigate any associations between benign prostate hyperplasia(BPH) and single nucleotide polymorphisms (SNPs) in the VDR gene (FokI, BsmI, ApaI and TaqαI loci) and theCYP17 gene (MspA1I locus), as well as TA repeat polymorphism in SRD5A2 gene among Lebanese men. Materialsand
Methods: DNA extracted from blood of 68 subjects with confirmed BPH and 79 age-matched controls wassubjected to PCR/PCR-restriction fragment length polymorphism analysis. The odds ra=tio (OR) of having agenotype and the relative risk (RR) of developing BPH for having the genotype were calculated and the alleleswere designated risk-bearing or protective.
Results: Our data indicated that the A and B alleles of the VDR ApaIand BsmI SNPs were highly associated with increased risk of BPH (p=0.0168 and 0.0002, respectively). Moreover,63% of the controls compared to 43% of the subjects with BPH were homozygous for none of the risk-bearingalleles (p=0.0123) whereas 60% of the controls and 28% of the subjects with BPH were homozygous for two ormore protective alleles (p<0.0001).
Conclusions: For the first time, our study demonstrated that ApaI and BsmIof the VDR gene are associated with risk of BPH among Lebanese men. Our study also indicated that overallpolymorphism profile of all the genes involved in prostate physiology could be a better predictor of BPH risk.