Comparison of Two Methods to Extract DNA from Formalin- Fixed, Paraffin-Embedded Tissues and their Impact on EGFR Mutation Detection in Non-small Cell Lung Carcinoma


Objective: Molecular pathology tests are often carried for clinicopathological diagnosis and pathologists haveestablished large collections of formalin-fixed, paraffin-embedded tissue (FFPE) banks. However, extraction ofDNA from FFPE is a laborious and challenging for researchers in clinical laboratories. The aim of this studywas to compare two widely used DNA extraction methods: using a QIAamp DNA FFPE kit from Qiagen and aCobas Sample Preparation Kit from Roche, and evaluated the effect of the DNA quality on molecular diagnostics.
Methods: DNA from FFPE non-small cell lung carcinoma tissues including biopsy and surgical specimens wasextracted with both QIAamp DNA FFPE and Cobas Sample Preparation Kits and EGFR mutations of nonsmallcell lung carcinomas were detected by real-time quantitative PCR using the extracted DNA. Results and
Conclusion: Our results showed that DNA extracted by QIAamp and Cobas methods were both suitable to detectdownstream EGFR mutation in surgical specimens. Howover, Cobas method could yield more DNA from biopsyspecimens, and gain much better EGFR mutation results.