High-risk (HR) human papillomavirus (HPV) testing is important in cervical cancer screening for triagecolposcopy. The objective of the study was to evaluate the prevalence of HR HPV infection with different cervicalcytological features among women undergoing health examination. A total of 2,897 women were retrospectivelyevaluated between May 2011 to December 2011. DNA was extracted from residual specimens collected duringroutine liquid-based cytology tests at the National Cancer Institute. Overall, HR HPV prevalence was 9.3%including 1.6% of HPV-16 and 0.4% of HPV-18. Of all 270 HPV positive samples, 211 (78.1% were HR-HPVnon 16/18; 47 (17.4%) were HPV-16 and 12 (4.4%) were HPV-18. The prevalence of HPV infection was similarin all age groups, although a higher rate was observed in women age 31-40 years. Among women with normalcytology, HR HPV positive were found in 6.7%. In abnormal cytology, HR HPV were found 46.7% in atypicalsquamous cells (ASC), 54.8% in low-grade squamous intraepithelial lesions (LSIL) and 80.0% in high-gradesquamous intraepithelial lesions (HSIL). HPV-16 was detected in 8.6%, 6.4% and 12.0% of ASC, LSIL andHSIL, respectively. The results of this study provide baseline information on the HPV type distribution, whichmay be useful for clinicians to decide who should be monitored or treated more aggressively.