RASSF1A Suppresses Proliferation of Cervical Cancer Cells


Background: This study aimed to explore the effects of ras association domain family 1 A (RASSF1A) onproliferation and apoptosis of human cervical cancer cell line Hela cells. Materials and
Methods: RASSF1Awas cloned into the pcDNA3.1(+) vector to generate pcDNA3.1(+)-RASSF1A plasmid for transfection into Helacells. Changes in the proliferation and apoptosis of cultured Hela cells were examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium chloride assay and flow cytometry. A protein array was used to analyzethe expression of apoptotic factors.
Results: Plasmid pcDNA3.1(+)-RASSF1A was generated and transfectedinto Hela cells to stably express RASSF1A in Hela cells. RASSF1A transfection was effective in inhibiting theproliferation of Hela cells up to 52.4%, as compared to cells transfected with an empty plasmid. RASSF1Aexpression also successfully induced apoptosis in human cervical cells with an apoptosis rate of 20.5%. Moreimportantly, protein array results showed that RASSF1 A transfection induced overexpression of p21 and caspase8, while decreasing the expression of survivin in Hela cells.
Conclusions: RASSF1A expression was effective insuppressing the proliferation and increasing apoptosis of Hela cells, and may be a potential therapy for cervicalcancer in clinic.