Preparation of 5-fluorouracil-loaded Nanoparticles and Study of Interaction with Gastric Cancer Cells


Aims: To prepare 5-fluorouracil (5-Fu) nanoparticles with higher encapsulation efficiency and drug loading,and then investigate interaction with the SGC-7901 gastric cancer cell line. Materials and
Methods: Prescriptionwas optimized by orthogonal experiments, the encapsulation efficiency and loading capacity were tested by highperformanceliquid chromatography, and inhibition of proliferation by 5-Fu nanoparticles and 5-Fu given tocells for 24, 48 and 72 hours was investigated by methyl thiazolyl tetrazolium assay (MTT). In addition, 5-Funanoparticles were labeled by fluorescein isothiocyanate (FITC), and absorption into cells was tested by flowcytometry.
Results: The optimal conditions for preparation were concentrations of 5-Fu of 5mg/ml, of CaCl2of 60 mg/ml and of chitosan of 2 mg/ml. With a stirring speed of 1200rpm, encapsulation efficiency of 5-Funanoparticles was 55.4±1.10% and loading capacity was 4.22±0.14%; gastric cancer cells were significantlyinhibited by 5-Fu nanoparticles in a time and concentration dependent manner, and compared to 5-Fu withslower drug release, in a certain concentration range, inhibition with 5-Fu nanoparticles was stronger. 5-Funanoparticles were absorbed by the cells in line with the concentration.
Conclusions: 5-Fu nanoparticles can inhibitgrowth of gastric cancer cells in vitro to a greater extent than with 5-Fu with good adsorption characteristics,supporting feasibility as a carrier.