MicroRNAs (miRNAs) are ubiquitously expressed small, non-coding RNAs that negatively regulate geneexpression at a post transcriptional/translational level. They have emerging as playing crucial roles in cancerat all stages ranging from initiation to metastasis. As a tumor suppressor miRNA, aberrant expression ofmicroRNA-29b (miR-29b) has been detected in various types of cancer, and its disturbance is related withtumor development and progression. In this review, we summarize the latest findings with regard to the tumorsuppressor signatureof miR-29b and its regulatory mechanisms. Our review highlights the diverse relationshipsbetween miR-29b and its target genes in malignant tumors.