Neuroblastoma is the most common extracranial solid tumor in children. Approximately half of the affectedpatients are diagnosed with high-risk poor prognosis disease, and novel therapies are needed. Sanguinarine is abenzophenanthridine alkaloid which has anti-microbial, anti-oxidant and anti-inflammatory properties. The aimof this study is whether sanguinarine has in vitro apoptotic effects and which apoptotic genes might be affected inthe human neuroblastoma cell lines SH-SY5Y (N-myc negative), Kelly (N-myc positive, ALK positive), and SKN-BE(2). Cell viability was analysed with WST-1 and apoptotic cell death rates were determined using TUNEL.After RNA isolation and cDNA conversion, expression of 84 custom array genes of apoptosis was determined.Sanguinarine caused cell death in a dose dependent manner in all neuroblastoma cell lines except SK-N-BE(2)with rates of 18% in SH-SY5Y and 21% in Kelly human neuroblastoma cells. Cisplatin caused similar apoptoticcell death rates of 16% in SH-SY5Y and 23% in Kelly cells and sanguinarine-cisplatin combinations caused thesame rates (18% and 20%). Sanguinarine treatment did not affect apoptototic gene expression but decreasedlevels of anti-apoptotic genes NOL3 and BCL2L2 in SH-SY5Y cells. Caspase and TNF related gene expressionwas affected by the sanguinarine-cisplatin combination in SH-SY5Y cells. The expression of regulation ofapoptotic genes were increased with sanguinarine treatment in Kelly cells. From these results, we conclude thatsanguinarine is a candidate agent against neuroblastoma.