Effects of TNF Secreting HEK Cells on B Lymphocytes’ Apoptosis in Human Chronic Lymphocytic Leukemias


Background: Tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL) is an antitumorcandidate in cancer therapy. This study focused on effects of TRAIL, as a proapototic ligand that causes apoptosis,in B-CELL chronic lymphocytic leukemia cells (B-CLL) . Materials and
Methods: A population of HEK 293cells was transducted by lentivirus that these achieved ability for producing the TRAIL protein and then HEK293 cells transducted were placed in the vicinity of CLL cells. After 24 hours of co-culture, apoptosis of CLLcells was assessed by annexin V staining.
Results: The amount of Apoptosis was examined separately in fourgroups: 293 HEK TRAIL (16.17±1.04%); 293 HEK GFP (2.7±0.57%); WT 293 HEK (2±2.6%); and CLL cells(0.01±0.01%). Among the groups studied, the maximum amount of apoptosis was in the group that the vectorencoding TRAIL was transducted. In this group, the mean level of soluble TRAIL in the culture medium was253pg/ml; also flow cytometry analyzes showed that proapotosis in this group was 32.8±1.6%, which was higherthan the other groups.
Conclusions: In this study, we have demonstrated that TNF secreted from HEK 293 cellsare effective in death of CLL cells.