MiR-323-5p acts as a Tumor Suppressor by Targeting the Insulin-like Growth Factor 1 Receptor in Human Glioma Cells


Background: MicroRNAs, small noncoding RNA molecules, can regulate mammalian cell growth, apoptosisand differentiation by controlling the expression of target genes. The aim of this study was to investigate thefunction of miR-323-5p in the glioma cell line, U251. Materials and
Methods: After over-expression of miR-323-5p using miR-323-5p mimics, cell growth, apoptosis and migration were tested by MTT, flow cytometry and cellwound healing assay, respectively. We also assessed the influence of miR-323-5p on the mRNA expression of IGF-1R by quantitative real-time reverse transcriptase PCR (qRT-PCR), and on the protein levels by Western blotanalysi. In addition, dual-luciferase reporter assays were performed to determine the target site of miR-323-5pto IGF-1R 3’UTR.
Results: Our findings showed that over-expression of miR-323-5p could promote apoptosisof U251 and inhibit the proliferation and migration of the glioma cells.
Conclusions: This study demonstratedthat increased expression of miR-323-5p might be related to glioma progression, which indicates a potential roleof miR-323-5p for clinical therapy.