The aim of this study was to evaluate the effects of grape juice on colon carcinogenesis induced by azoxymethane(AOM) and expression of NF-kB, iNOS and TNF- α.
Methods: Forty male Wistar rats were divided into 7 groups:G1, control; G2, 15 mg/kg AOM; G3, 1% grape juice 2 weeks before AOM; G4, 2% grape juice 2 weeks beforeAOM; G5, 1% grape juice 4 weeks after AOM; G6, 2% grape juice 4 weeks after AOM; G7, 2% grape juicewithout AOM. Histological changes and aberrant crypt foci (ACF) were studied, while RNA expression of NFkB,TNF- and iNOS was evaluated by qPCR.
Results: The number of ACF was higher in G2, and G4 presenteda smaller number of crypts per focus than G5 (p=0.009) and G6. Small ACF (1-3) were more frequent in G4compared to G2, G5 and G6 (p=0.009, p=0.009 and p=0.041, respectively). RNA expression of NF-kB was lowerin G3 and G4 compared to G2 (p=0.004 and p=0.002, respectively). A positive correlation was observed betweenTNF- α and NF-kB gene expression (p=0.002). In conclusion, the administration of 2% grape juice before AOMreduced the crypt multiplicity, attenuating carcinogenesis. Lower expression of NF-kB was observed in animalsexposed to grape juice for a longer period of time, regardless of concentration.