Matrix Metalloproteinase-9 -1562T Allele and its Combination with MMP-2 -735 C Allele are Risk Factors for Breast Cancer

Abstract

Background: Expression of matrix metalloproteinases (MMPs) is up-regulated in human cancers. The aimof present study was to investigate the role of MMP-9 C-1562T polymorphism and its interaction with MMP-2 C-735T polymorphism in susceptibility to breast cancer in a population from Western Iran with Kurdishethnic background. Materials and
Methods: The study sample of 205 individuals consisted of 101 breast cancerpatients and 104 healthy subjects. MMP-9 C-1562T and MMP-2 C-735T variants were identified using thepolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Results: Among67.4% of studied patients the breast cancer developed in the third and forth decades of the life. The frequencyof MMP-9 T allele was 17.3% in patients and 10.1% in controls. The presence of T allele significantly increasedthe risk of breast cancer by 1.87-fold [OR=1.87 (95% CI 1.05-3.33, p=0.035)]. The frequency of MMP-9 CT+TTgenotype tended to be higher in those patients with a family history of cancer in first degree-relatives (36.8%)than those without a family history (28.3%, p=0.37). We observed an interaction between the MMP-9 -1562 Tallele with MMP-2 -735 C allele that significantly increased the risk of breast cancer [OR=1.42 (95% CI 1.02-1.98, p=0.036)].
Conclusions: The present study demonstrated that MMP-9 C-1562T polymorphism alone andin combination with MMP-2 C-735T polymorphism increased the risk of breast cancer that might be a usefulbiomarker in identifying women at risk of developing breast cancer. Also, this study revealed that in most womenfrom Western Iran breast cancer presents in third and fourth decades of life.

Keywords