Infection of the uterine cervix by human papilloma viruses (HPV) may be associated with cervical pre-cancerand invasive cervical carcinoma if left untreated. With advance in molecular techniques, it has become easier todetect the resence of HPV DNA long before the appearance of any lesion. This study concerned cervical scrapesamples of 310 married non-pregnant women attending a gynecology outpatient department for both Pap andPCR testing to detect HPV DNA. Nested PCR using primers for L1 consensus gene with My9/My11 and GP6+/GP5+followed by multiplex PCR were carried out to detect HPV 16 and HPV18.
Result: HPV prevalence was11.9% out of which 3.67% cases of negative for intra-epithelial lesion or malignancy (NILM) and in 71.1%(27/38) of atypical cervical smears were HPV positive. There was increasing trend of high-risk-HPV positivity(HR HPV 16 and 18), from 20% in benign cytology (NILM) to 42.9 % in LSIL, 71.41% in HSIL and 100% inSCC. There was highly significant association of HPV infection with cervical lesion (x2=144.0, p<0.01) and alsowith type specific HPV prevalence (x2=7.761*(p<0.05).