Objective: To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and itsinteraction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province.Materials and
Methods: A case series study (case-case) was conducted. Questionnaire data were collected and3 ml-5ml venous blood was drawn for DNA extraction among the qualified research subjects. PLCE1 genepolymorphism was detected after PCR amplification of DNA. SPSS 13.0 software was used for statistical analysisof the data.
Results: The three genotypes A/A, A/G and G/G PLCE1 gene rs2274223 was 31, 16 and 4 cases,accounting for 60.8%, 31.4%, 0.08% respectively. The difference of three genotypes (AA/GA/GG) proportionbetween negative and positive family history of patients was statistically significant, χ2=6.213, p=0.045. There wasno statistically significant relationship between PLCE1 gene rs2274223 polymorphism and smoking, drinking,χ2=0.119, p=0.998, and χ2=1.727, p=0.786. There was no linkage of the three rs2274223 PLCE1 gene genotypes(AA/GA/GG) proportion with eating fried, pickled, hot, mildew, overnight, smoked, excitant food, eat speed, salttaste or not (p>0.05). or with living environment pollution and nine risk factors of occupational exposure (p>0.05).There was no statistically significant difference in TS scores between different genotype of rs2274223 PLCE1gene.
Conclusions: The PLCE1 rs2274223 polymorphism has a relationship with family history of esophagealcancer, but does not have any significant association with age, gender, smoking, alcohol drinking, food hygiene,eating habits, living around the environment and occupation in cases.