Lack of Influence of TP53 Arg72Pro and 16bp Duplication Polymorphisms on Risk of Breast Cancer in Iran


TP53 is assumed to be a very important tumour suppressor gene, as illustrated by recent reports that haveshown effects of its polymorphisms on breast cancer risk. Arg72Pro and PIN3(16bp duplication) polymorphismsare proposed to have an effective role in structural changes of p53 and have therefore attracted interest as a riskfactor for breast cancer in different populations. The aim of this study was to examine and determine whetherp53 codon 72 and PIN3 Ins16 bp may be associated with an increased risk for breast cancer in female patientsfrom the northwest of Iran. Genotyping was performed by PCR-RFLP (polymerase chain reaction-restrictionfragment length polymorphism) method for a total of 100 women with breast cancer and 100 healthy womenwithout any background of cancer, focusing on the TP53 Arg72Pro-16Del/Ins haplotypes and the combinedgenotypes. The results in this study established no statistical significant distinctions between the genotypes anda llele frequency were found for Arg72Pro and PIN3 Ins 16 bp polymorphisms between patients and controls.