Detection of BCR/ABL Fusion Gene by Hematological and Cytogenetical Analysis in Chronic Myeloid Leukemia Patients in Quetta, Pakistan


Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells,caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known asthe Philadelphia chromosome. Materials and
Methods: A total of 51 CML patients were recruited in this study.Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin andplatelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrowsamples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms.
Results: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4(8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 103/μl, hemoglobin 10.44 mg/dl, and platelets288.6 103/μl were observed in this study.
Conclusions: In this study, Ph positive translocation between chromosome(9:22)(q34;q11) were observed in 40 (88.9%) CML patients.