Gastric cancer (GC) is one of the most common malignancies in the world. It is the first cause of cancerdeaths in both sexes In Iranian population. Circulating insulin-like growth factor-one (IGF-1) levels have beenassociated for gastric cancer. IGF-1 protein has central roles involved in the regulation of epithelial cell growth,proliferation, transformation, apoptosis and metastasis. Single nucleotide polymorphism in IGF-1 regulatoryelements may lead to alter in IGF-1expression level and GC susceptibility. The aim of this study was to investigatethe influence of IGF-1 gene polymorphism (rs5742612) on risk of GC and clinicopathological features for thefirst time in Iranian population. In total, 241 subjects including 100 patients with GC and 141 healthy controlswere recruited in our study. Genotypes were analyzed using polymerase chain reaction-restriction fragmentlength polymorphism (PCR-RFLP) assay with DNA from peripheral blood. The polymorphism was statisticallyanalyzed to investigate the relationship with the risk of GC and clinicopathological properties. Logistic regressionanalysis revealed that there was no significant association between rs5742612 and the risk of GC. In addition,no significant association between genotypes and clinicopathological features was observed (p value>0.05). Thefrequencies of the CC, CT, and TT genotypes were 97%, 3%, and 0%, respectively, among the cases, and 97.9%,2.1%, and 0%, respectively, among the controls. CC genotype was more frequent in cases and controls. Thefrequencies of C and T alleles were 98.9% and 1.1% in controls and 98.5% and 1.5% in patient respectively.Our results provide the first evidence that this variant is rare in Iranian population and it may not be a powerfulgenetic predisposing biomarker for prediction GC clinicopathological features in an Iranian population.