Helicobacter pylori (H. pylori) infection as a serious problem in both adults and children can induce chronicgastritis, peptic ulcer disease (PUD), and possibly gastric cancer. The aim of the current study was to surveyantibiotic resistance and also to determine influence of PPARγ polymorphism in patients with H. pylori infection.During an 11-month-period, 98 H. pylori isolates were collected from 104 biopsy specimens. In vitro susceptibilityof H. pylori isolates to 4 antimicrobial agents metronidazole, clarithromycin, amoxicillin and tetracycline wereassessed by quantitative method according to European Committee on Antimicrobial Susceptibility Testing(EUCAST) guideline. PPARγ polymorphism was determined using polymerase chain reaction-restrictionfragment length polymorphism assay. The frequency of H. pylori infection in our study was 94.2%. In vitrosusceptibility data showed that highest level of resistance was related to metronidazole (66.3%), and the majorityof H. pylori isolates were highly susceptible to amoxicillin and tetracycline (94.9% and 96.9%, respectively).Genotypic frequencies were 25.5% for CC (Pro12Pro), 40.8% for GC (Pro12Ala) and 33.7% for GG (Ala12Ala).In our study, CG genotype had highest distributions among infected patients with H. pylori. The study suggeststhat the PPAR-γ Pro12Ala polymorphism could be evaluated as a potential genetic marker for susceptibility togastric cancer in the presence of H. pylori infection.