Micronutrients in food have been found to have chemopreventive effects, supporting the conclusions fromepidemiologie studies that consumption of fresh fruits and vegetables reduces cancer risk. The present studywas carried out to evaluate the role of querctin (Q) and sodium gluconate (GNA) supplementation separatelyor in combination in ameliorating promotion of colon tumor development by dimethyl-hydrazine (DMH) inmice. Histopathological observation of colons in mice treated with DMH showed goblet cell dysplasia withinflammatory cell infiltration. This pathological finding was associated with significant alteration in oxidativestress markers in colon tissues and carcinoembryonic antigen (CEA) levels in plasma. Mice co-treated with GNAand Q showed mild changes of absorptive and goblet cells and inflammatory cell infiltration in lamina properia,with improvement in oxidative stress markers. In conclusion, findings of the present study indicate significantroles for reactive oxygen species (ROS) in pathogenesis of DMH-induced colon toxicity and initiation of coloncancer. Also, they suggest that Q, GNA or the combination of both have a positive beneficial effect against DMHinduced colonic cancer induction in mice.