Association of the -2518 A/G Polymorphism of MCP-1 with Breast Cancer in Punjab, North-West India


Background: Monocyte chemoattractant protein-1 (MCP-1) is a major chemokine thought to be responsiblefor monocyte and T-lymphocyte recruitment in acute inflammatory conditions and recruitment of macrophagesin tumors. It is also implicated in cardiovascular disease, rheumatoid arthritis and chronic obstructive pulmonarydisease. The aim of the present study was to investigate the correlation between MCP-1 -2518 A/G polymorphismand breast cancer risk in patients from Amritsar city of Punjab state in North-West India. Materials and
Methods:We screened DNA samples of 200 sporadic breast cancer patients and 200 age and gender matched unrelatedhealthy individuals for MCP-1 -2518 A/G polymorphism using the PCR-RFLP method.
Results: A significantlyincreased frequency of the GG genotype was observed in patients as compared to controls. Individuals carryingthe MCP1 -2518GG genotype had a two fold risk for breast cancer (OR=2.06, 95%CI, 1.06-3.98; p=0.03). Geneticmodels analysis revealed a significant association between MCP-1 -2518 A/G polymorphism and cancer riskin homozygous co-dominant (OR=2.06, 95%CI, 1.06-3.98; p=0.03) and recessive (OR=1.97, 95%CI, 1.05-3.70;p=0.03) models.
Conclusions: We conclude that the GG genotype of the MCP-1-2518 A/G polymorphism isassociated with increased risk to breast cancer in Punjab, North-West India.