Department of Heavy Ion Radiation Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Gansu Province, China
Abstract
The effects of carbon-ion irradiation on cancer cell telomere function have not been comprehensively studied. In our previous report cancer cells with telomere dysfunction were more sensitive to carbon-ion irradiation, but the underlying mechanisms remained unclear. Here we found that telomerase activity was suppressed by carbon-ion irradiation via hTERT down-regulation. Inhibition of telomere activity by MST-312 further increased cancer cell radiosensitivity to carbon-ion radiation. hTERT suppression caused by either carbon-ion irradiation or MST-312 impaired mitochondrial function, as indicated by decreased membrane potential, mtDNA copy number, mitochondrial mass, total ATP levels and elevated reactive oxygen species (ROS). PGC-1 expression was repressed after carbion-ion irradiation, and hTERT inhibition by MST-312 could further exacerbate this effect. Lowering the mitochondrial ROS level by MitoTEMPO could partially counteract the induction of cellular senescence induced by carbon-ion radiation and MST-312 incubation. Taken together, the current data suggest that telomere-mitochondrion links play a role in the induction of senescence in MCF-7 cells after carbon-ion irradiation.
Miao, G., Zhou, X., Zhang, X., Xie, Y., Sun, C., Liu, Y., Gan, L., & Zhang, H. (2016). Telomere-Mitochondrion Links Contribute to Induction of Senescence in MCF-7 Cells after Carbon-Ion Irradiation. Asian Pacific Journal of Cancer Prevention, 17(4), 1993-1998.
MLA
Guo-Ying Miao; Xin Zhou; Xin Zhang; Yi Xie; Chao Sun; Yang Liu; Lu Gan; Hong Zhang. "Telomere-Mitochondrion Links Contribute to Induction of Senescence in MCF-7 Cells after Carbon-Ion Irradiation". Asian Pacific Journal of Cancer Prevention, 17, 4, 2016, 1993-1998.
HARVARD
Miao, G., Zhou, X., Zhang, X., Xie, Y., Sun, C., Liu, Y., Gan, L., Zhang, H. (2016). 'Telomere-Mitochondrion Links Contribute to Induction of Senescence in MCF-7 Cells after Carbon-Ion Irradiation', Asian Pacific Journal of Cancer Prevention, 17(4), pp. 1993-1998.
VANCOUVER
Miao, G., Zhou, X., Zhang, X., Xie, Y., Sun, C., Liu, Y., Gan, L., Zhang, H. Telomere-Mitochondrion Links Contribute to Induction of Senescence in MCF-7 Cells after Carbon-Ion Irradiation. Asian Pacific Journal of Cancer Prevention, 2016; 17(4): 1993-1998.
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