Preparation, Characterization and Cytotoxicity of Silibinin- Containing Nanoniosomes in T47D Human Breast Carcinoma Cells


Chemistry faculty, Islamic Azad University of Shahrood, Semnan, Iran


Background Breast cancer is one of the most frequent cancer types within female populations. Silibinin is a chemotherapeutic agent ative against cancer. Niosomes are biodegradable, biocompatible, safe and effective carriers for drug delivery. ObjectiveTo prepare nanoniosomal silibinin and evaluate its cytotoxicity inthe T-47D breast cancer cell line. Materials and Methods Niosomes were prepared by reverse phase evaporation of a mixture of span 20, silibinin, PEG-2000 and cholesterol in chloroform and methanol solvent (12 v/v). The solvent phase was evaporated using a rotary evaporator and the remaining gel phase was hydrated in phosphate buffer saline. Mean size, size distribution and zeta potential of niosomes were measured with a Zetasizer instrument and then nanoparticles underwent scanning electron microscopy. The drug releasing pattern was evaluated by dialysis and the cytotoxicity of nanoniosomes in T-47D cells was assessed by MTT assay. Results Particle size, size variation and zeta potential of the niosomal nanoparticles were measured as 178.4 5.4 nm, 0.38 0.09 and -15.3 1.3 mV, respectively. The amount of encapsulated drug and the level of drug loading were determined 98.6 2.7% and 22.3 1.8%, respectively; released drug was estimated about 18.62.5% after 37 hours. The cytotoxic effects of nanoniosome were signi cantly increased when compared with the free drug. Conclusions This study nding suggests that silibinin nanoniosomes could serve as a new drug formulation for breast cancer therapy.