1Lebanese Atomic Energy Commission, Beirut, Lebanon; Department of Biochemistry, Lebanese University, Hadath, Lebanon; and Department of Chemistry, Utah Valley University, Orem, UT, USA.
2Department of Biology, Utah Valley University, Orem, UT, USA
3Lebanese Atomic Energy Commission, Beirut, Lebanon
4Department of Urology, Al-Salam Hospital, Tripoli, Lebanon
5Office of Academic Research Support, Utah Valley University, Orem, Utah, USA
6800W Univ Pkway
Aims: The goal of the study was to investigate possible associations of some single nucleotide polymorphisms (SNPs) in the VDR gene (the FokI, BsmI, ApaI and TaqαI loci), and the CYP17 gene (the MspA1I locus), and variable numbers of TA repeats in the SRD5A2 gene, with prostate cancer (PCa) among Lebanese men. Materials and Methods: Blood DNA samples of 50 subjects with confirmed PCa and 79 age-qualified controls were subjected to PCR or PCR-RFLP analyses, and risk-bearing and protective alleles were identified. The odds ratio (OR) of having a genotype and the relative risk (RR) of developing PCa were calculated. In addition, the distribution of homozygosis in the risk-bearing and protective alleles were compared between the control and the PCa groups. Results: The A and B alleles of the VDR ApaI and BsmI loci and the 0 TA repeat allele of the SRD5A2 gene were found to be associated with increased risk of PCa (p = 0.022, 0.029 and 0.013, respectively). A higher fraction of the subjects with PCa compared to the controls was homozygous for two or more of the risk-bearing alleles (46% for PCa, 27% for controls, p = 0.023). In contrast, a higher fraction of the controls compared to the PCa group was homozygous in two or more of the protective alleles (71% for controls, 38% for PCa group, p = 0.001). Conclusions: To the best of our knowledge, this is the first genetic study demonstrating any association of polymorphisms of the VDR and SDR5A2 genes with increased risk of PCa among Lebanese men. Our study also indicated that the overall polymorphism profile of all genes involved in prostrate physiology is likely to be a better indicator for PCa risk than polymorphisms in individual genes.