VEGFR-3 Expression in Oral Lichen Planus

Document Type: Research Articles

Authors

1 Oral and Maxillofacial Pathology Department,Dental Faculty, Babol University of Medical Sciences, Babol, Iran

2 Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran

3 pathology department, Medical Faculty, Babol University of Medical Sciences, Babol, Iran

4 Non-Communicable pediatrics Diseases Research Center, Babol University of Medical Sciences, Babol, Iran

5 Oral and Maxillofacial Pathology Department, Dental Faculty, Mazandaran University of Medical Sciences, Sari, Iran

6 Oral and Maxillofacial Pathology Department, Dental Faculty, Tehran University of Medical Sciences, Tehran, Iran

Abstract

 
Background and objective: Given the postulated the role of inflammation and possible contribution of lymphangiogenesis in oral lichen planus, this study aimed to assess any associated presence of VEGFR-3. Material and Methods: This cross-sectional study was performed on 52 formalin fixed and paraffin embedded blocks of oral lichen planus (pathological diagnosis based on Modified WHO criteria), comprising 25 of erosive and 27 of reticular type, along with 60 samples of normal mucosa (with minimal inflammation from clinical and histopathological aspects) obtained at crown lengthening surgery. Four micron sections were cut from paraffin blocks and stained with H and E for confirmation of diagnosis and by immunohistochemistry with primary antibodies against VEGFR-3. Negative controls were provided by omission of primary antibody and placenta was considered as a positive control. Data were analyzed by Chi-square, Mann-Whitney and Kruskal-wallis tests and P Findings: VEGFR-3 expression was apparent in 61.5% of lichen planus specimens and 5% of those from normal mucosa (p<0.001). Also, the average number of stained vessels was significantly higher in oral lichen planus than in normal mucosa (p<0.001). VEGFR-3 expression in oral lichen planus (p=0.262) and the average number of stained vessels (p=0.092) demonstrated no significant difference according to the type. Conclusion: It appears that VEGFR-3 expression might be involved in the pathogenesis of the oral lichen planus through increasing lymphatic vessels and lymphangiogenesis

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