Application of Pineapple Juice in the Fish Digestion Process for Carcinogenic Liver Fluke Metacercaria Collection

Document Type : Research Articles


1 Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand

2 Neglected, Zoonosis and Vector-Borne Disease Group, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand

3 Liver Fluke and Cholangiocarcinoma Research Center, Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, Khon Kaen 40002, Thailand

4 Department of Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, Thailand


Pepsin is common digestive enzyme used for fish digestion in the laboratory to collect trematode metacercariae. In a field study, to survey the infected fish is needed a huge yield of pepsin and it is very expensive. Therefore, our purpose of this study was to investigate the candidate enzyme from pineapple juice which has a digestive enzyme called bromelain, a mixture of proteolytic enzymes, to digest fish in order to harvest metacercariae. Fish were divided into 2 groups: one group in which metacercariae were harvested using acid pepsin as a control and other groups in which the fish was digested using fresh pineapple juices. The results showed that pineapple juice is able to digest fish similarly to pepsin. The Pattavia pineapple juice had the highest number of metacercariae similar to the control. For Trat Si Thong pineapple juice,we found the number of metacercariae was less than control. This result suggests that the Pattavia pineapple juice was optimal juice for fish digestion to metacercaria collection and can be used instread of pepsin acid.


Main Subjects

Volume 18, Issue 3
March 2017
Pages 779-782
  • Receive Date: 12 February 2017
  • Revise Date: 06 March 2017
  • Accept Date: 16 April 2017
  • First Publish Date: 16 April 2017